4861864 Rotavirus antigens
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PATENT ABSTRACTS 365 A novel gene coding for thermostable beta- tion. A novel feline interferon is produced in cul- galactosidase, novel recombinant DNA in which ture with cells infected with the defective non- a DNA fragment containing the above gene is in- infectious retroviruses. serted, novel Bacillus subtilis in which the above recombinant DNA is introduced, and novel thermostable beta-galactosidase obtained by 4861722 cultivating the above Bacillus subtilis. CORYNEFORM BACTERIA CARRYING RECOMBINANT 4861719 PLASMIDS AND THEIR USE IN THE FERMENTATIVE DNA CONSTRUCTS FOR PRODUCTION OF L-LYSINE RETROVIRUS PACKAGING CELL LINES Konosuke Sano, Koichi Ito, Kiyoshi Miwa, Shigeru Nakamori, Tokyo, Japan assigned to A Dusty Miller assigned to Fred Hutchinson Ajinomoto Company ~nc Cancer Research Center A genetic sequence coding for the production of DNA constructs consisting essentially of the a protein having the activity of diaminopimelic promoter, gag, pol, and env sequences of a hel- acid decarboxylase and having two Pst I per virus useful for making retrovirus packaging cleavage sites in its DNA chain and a molecular cell lines that do not yield helper virus and do not weight of 2.9 +/-0.05 Md, is incorporated into a transfer the packaging function. Such DNA vehicle capable of replication in Coryneform molecules are constructed by deleting from the bacteria and used to produce L:lysine by fermen- genome of a replication-competent retrovirus all tation. cis-acting elements except for the tRNA binding site. Specifically, deletion is made of the pack- 4861864 aging signal, the site for initiation of second strand DNA synthesis, the site required for translation of reverse transcriptase during first ROTAVIRUS ANTIGENS strand DNA synthesis, and the provirus integra- tion signal. DNA construct pPAM3 (ATCC No. Paul H Atkinson, A Richard Bellamy, Gerald W 40234) is a representative embodiment. A cell Both, Marianne P0ruchynsky assigned to Albert line containing such an altered viral genome does Einstein College of Medicine of Yeshiva Univer- not transmit this virus or transfer the packaging sity (a Div of Yeshiva University) signal, but will transmit high titers of other viral RNAs containing the proper cis-acting elements, A soluble form of the neutralizing antigen or including retroviral vectors designed to carry rotavirus is provided. The antigen is formed by foreign genes. Cell line PA317 (ATCC No. CLR clonin*g DNA coding for VP7 protein into an ex- 9078) is a representative embodiment, pression vector and preparing deletion mutants coding for a soluble cell secreted neutralizing antigen. The preparation of the deletion mutants includes the removal of the DNA sequences 4861'7'20 coding for those amino acids of the VP7 protein responsible for its normal intracellular location ONCORNAVIRUS VACCINES AND and non-secretion characteristics. The deletion FELINE ALPHA-TYPE mutant may be used in the formation of stably INTERFERON transformed animal cell lines which contiuously secrete the antigen, or in the production of cer- Neils C Pedersen, Janet Yamamoto assigned to tain yeast strains which produce the soluble anti- Regents of the University of California gen with appropriate glycosylation. Retroviral vaccines are provided comprising in- 4861868 competent retroviruses containing defective RNA produced by growing viral transformed cells in the presence of interferon. The resulting PRODUCTION OF PROTEINS IN defective viruses by themselves or in combina- PROCARYOTES tion with interferon can be used as vaccines for immunizing viral sensitive hosts against infec- Gwen G Krivi assigned to Monsanto Company
Transcript of 4861864 Rotavirus antigens
PATENT ABSTRACTS 365
A novel gene coding for thermostable beta- tion. A novel feline interferon is produced in cul- galactosidase, novel recombinant DNA in which ture with cells infected with the defective non- a DNA fragment containing the above gene is in- infectious retroviruses. serted, novel Bacillus subtilis in which the above recombinant DNA is introduced, and novel thermostable beta-galactosidase obtained by 4861722 cultivating the above Bacillus subtilis.
C O R Y N E F O R M B A C T E R I A C A R R Y I N G R E C O M B I N A N T
4861719 P L A S M I D S A N D T H E I R U S E I N T H E F E R M E N T A T I V E
D N A C O N S T R U C T S F O R P R O D U C T I O N O F L - L Y S I N E R E T R O V I R U S P A C K A G I N G C E L L
L I N E S Konosuke Sano, Koichi Ito, Kiyoshi Miwa, Shigeru Nakamori, Tokyo, Japan assigned to
A Dusty Miller assigned to Fred Hutchinson Ajinomoto Company ~nc Cancer Research Center
A genetic sequence coding for the production of DNA constructs consisting essentially of the a protein having the activity of diaminopimelic promoter, gag, pol, and env sequences of a hel- acid decarboxylase and having two Pst I per virus useful for making retrovirus packaging cleavage sites in its DNA chain and a molecular cell lines that do not yield helper virus and do not weight of 2.9 +/-0.05 Md, is incorporated into a transfer the packaging function. Such DNA vehicle capable of replication in Coryneform molecules are constructed by deleting from the bacteria and used to produce L:lysine by fermen- genome of a replication-competent retrovirus all tation. cis-acting elements except for the tRNA binding site. Specifically, deletion is made of the pack- 4861864 aging signal, the site for initiation of second strand DNA synthesis, the site required for translation of reverse transcriptase during first R O T A V I R U S A N T I G E N S strand DNA synthesis, and the provirus integra- tion signal. DNA construct pPAM3 (ATCC No. Paul H Atkinson, A Richard Bellamy, Gerald W 40234) is a representative embodiment. A cell Both, Marianne P0ruchynsky assigned to Albert line containing such an altered viral genome does Einstein College of Medicine of Yeshiva Univer- not transmit this virus or transfer the packaging sity (a Div of Yeshiva University) signal, but will transmit high titers of other viral RNAs containing the proper cis-acting elements, A soluble form of the neutralizing antigen or including retroviral vectors designed to carry rotavirus is provided. The antigen is formed by foreign genes. Cell line PA317 (ATCC No. CLR clonin*g DNA coding for VP7 protein into an ex- 9078) is a representative embodiment, pression vector and preparing deletion mutants
coding for a soluble cell secreted neutralizing antigen. The preparation of the deletion mutants includes the removal of the DNA sequences
4861'7'20 coding for those amino acids of the VP7 protein responsible for its normal intracellular location
O N C O R N A V I R U S V A C C I N E S A N D and non-secretion characteristics. The deletion F E L I N E A L P H A - T Y P E mutant may be used in the formation of stably
I N T E R F E R O N transformed animal cell lines which contiuously secrete the antigen, or in the production of cer-
Neils C Pedersen, Janet Yamamoto assigned to tain yeast strains which produce the soluble anti- Regents of the University of California gen with appropriate glycosylation.
Retroviral vaccines are provided comprising in- 4861868 competent retroviruses containing defective RNA produced by growing viral transformed cells in the presence of interferon. The resulting P R O D U C T I O N O F P R O T E I N S I N defective viruses by themselves or in combina- P R O C A R Y O T E S tion with interferon can be used as vaccines for immunizing viral sensitive hosts against infec- Gwen G Krivi assigned to Monsanto Company
