4547280 Maltose sensor
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4546086 MICROBIAL CULTURE APPARATUS Melvin W Hounsell A microbial culture apparatus including an outer sealable enclosure for receiving a culture retaining container and a self-contained anaerobic gassing and indicator apparatus which can be selectively activated to provide a gaseous atmosphere around the culture retaining container. The gassing and indicator apparatus includes an open top receptacle having a first rupturable container for producing a gaseous at- mosphere, a second rupturable container con- taining a color indicator, and a catalyst, and a cover for the receptacle arranged to rupture both rupturable containers when the cover is moved to a closed position to activate the gassing and indicating apparatus. The outer enclosure com- prises an outer open top receptacle for receiving the culture retaining container and the gassing and indicator apparatus, and a cover which can be moved to a closed position sealing the outer receptacle and activating the gassing and in- dicating apparatus. PATENT ABSTRACTS 151 specific ligand in a fluid medium suspected of containing the ligand. The method of the present invention involves the formation of a liquid reac- tion mixture comprising an enzyme, a ligand- specific binding component, a conjugate comprising ligand coupled to a modulator label, and sample fluid medium suspected of con- taining ligand. Once the reaction mixture is for- med, enzyme activity is monitored and the presence and amount of ligand in the sample fluid medium is determined by changes in en- zyme activity. Novel compositions useful in practicing the disclosed methods are provided. These include a biotin binding protein enzyme modulator, novel ligand-enzyme modulator conjugates, and biotin containing enzymes. The methods and compositions of the present inven- tion are particularly useful in the qualitative and quanutatwe identification of pharniaceuticals and any such antigenic units or sites, including haptens and fragments of large molecules pre- sent in such biologic fluids as blood, urine, spinal fluid, amniotic fluid, lymphatic fluid and the like. 4547280 MALTOSE SENSOR Yoshiharu Karasawa, Yoshinori Takata, Tokyo, Japan assigned to Hitachi Ltd In a method for assaying maltose to quantita- tively determine amylase, which comprises an enzyme membrane having immobilized alpha- glucosidase and glucose oxidase, the present in- vention is an improvement of the enzyme electrode for assaying maltose, where a hydro- gen peroxide electrode provided with a pal- ladium cathode is used. 4550075 METHOD FOR LIGAND DETERMINATION UTILIZING AN IMMUNOASSAY MONITORABLE BY BIOTIN-CONTAINING ENZYMES, AND COMPOSITIONS THEREFOR Cathy A Bacquet, Daniel Twumasi assigned to Kallestad Laboratories Inc Methods and compositions are disclosed for the qualitative and Quantitative identification of 4550163 LIGAND ANALOG-IRREVERSIBLE ENZYME INHIBITOR CONJUGATES Houston F Voss, Jacob Platmer, Thomas Herrin assigned to Abbott Laboratories The present invention encompasses a method for determining ligands in test samples com- prising intermixing with the test sample a ligand analog-irreversible enzyme inhibitor conjugate and a binding protein bindable to the ligand and the ligand analog-irreversible enzyme inhibitor conjugate and wherein the amount of ligand analog-irreversible enzyme inhibitor conjugate bound by the binding protein is related to the amount ofligand in the test sample, said binding protein reactivating the irreversible enzyme in- hibitor when bound to the ligand analog portion of the conjugate; intermixing an enzyme which is irreversibly inhibited by the ligand analog- irreversible enzyme inhibitor conjugate un- bound by the binding protein: and intermixing substrate to the enzyme and monitoring the en- zyme substrate reaction. The invention also in- cludes ligand analog-irreversible enzyme inhibitor conjugates useful as reagents in prac- ticing the method. Methods and reagents of the present are particularly useful in determining drugs, hormones, and the like in biological fluids.
Transcript of 4547280 Maltose sensor
4546086
M I C R O B I A L C U L T U R E A P P A R A T U S
Melvin W Hounsell
A microbial culture apparatus including an outer sealable enclosure for receiving a culture retaining container and a self-contained anaerobic gassing and indicator apparatus which can be selectively activated to provide a gaseous atmosphere around the culture retaining container. The gassing and indicator apparatus includes an open top receptacle having a first rupturable container for producing a gaseous at- mosphere, a second rupturable container con- taining a color indicator, and a catalyst, and a cover for the receptacle arranged to rupture both rupturable containers when the cover is moved to a closed position to activate the gassing and indicating apparatus. The outer enclosure com- prises an outer open top receptacle for receiving the culture retaining container and the gassing and indicator apparatus, and a cover which can be moved to a closed position sealing the outer receptacle and activating the gassing and in- dicating apparatus.
PATENT ABSTRACTS 151
specific ligand in a fluid medium suspected of containing the ligand. The method of the present invention involves the formation of a liquid reac- tion mixture comprising an enzyme, a ligand- specific binding component, a conjugate comprising ligand coupled to a modulator label, and sample fluid medium suspected of con- taining ligand. Once the reaction mixture is for- med, enzyme activity is monitored and the presence and amount of ligand in the sample fluid medium is determined by changes in en- zyme activity. Novel compositions useful in practicing the disclosed methods are provided. These include a biotin binding protein enzyme modulator, novel ligand-enzyme modulator conjugates, and biotin containing enzymes. The methods and compositions of the present inven- tion are particularly useful in the qualitative and quanutatwe identification of pharniaceuticals and any such antigenic units or sites, including haptens and fragments of large molecules pre- sent in such biologic fluids as blood, urine, spinal fluid, amniotic fluid, lymphatic fluid and the like.
4547280
M A L T O S E S E N S O R
Yoshiharu Karasawa, Yoshinori Takata, Tokyo, Japan assigned to Hitachi Ltd
In a method for assaying maltose to quantita- tively determine amylase, which comprises an enzyme membrane having immobilized alpha- glucosidase and glucose oxidase, the present in- vention is an improvement of the enzyme electrode for assaying maltose, where a hydro- gen peroxide electrode provided with a pal- ladium cathode is used.
4550075
M E T H O D F O R L I G A N D D E T E R M I N A T I O N U T I L I Z I N G A N I M M U N O A S S A Y M O N I T O R A B L E
BY B I O T I N - C O N T A I N I N G E N Z Y M E S , A N D C O M P O S I T I O N S
T H E R E F O R
Cathy A Bacquet, Daniel Twumasi assigned to Kallestad Laboratories Inc
Methods and compositions are disclosed for the qualitative and Quantitative identification of
4550163
L I G A N D A N A L O G - I R R E V E R S I B L E E N Z Y M E I N H I B I T O R
C O N J U G A T E S
Houston F Voss, Jacob Platmer, Thomas Herrin assigned to Abbott Laboratories
The present invention encompasses a method for determining ligands in test samples com- prising intermixing with the test sample a ligand analog-irreversible enzyme inhibitor conjugate and a binding protein bindable to the ligand and the ligand analog-irreversible enzyme inhibitor conjugate and wherein the amount of ligand analog-irreversible enzyme inhibitor conjugate bound by the binding protein is related to the amount ofligand in the test sample, said binding protein reactivating the irreversible enzyme in- hibitor when bound to the ligand analog portion of the conjugate; intermixing an enzyme which is irreversibly inhibited by the ligand analog- irreversible enzyme inhibitor conjugate un- bound by the binding protein: and intermixing substrate to the enzyme and monitoring the en- zyme substrate reaction. The invention also in- cludes ligand analog-irreversible enzyme inhibitor conjugates useful as reagents in prac- ticing the method. Methods and reagents of the present are particularly useful in determining drugs, hormones, and the like in biological fluids.
