3. Nucleic Acids Electrophoresis - Euroclone group€¦ · Nucleic Acids Electrophoresis 3 17...

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3. Nucleic Acids Electrophoresis Agaroses Acrylamides Buffers & Reagents for DNA Electrophoresis DNA Ladders Horizontal Electrophoresis System

Transcript of 3. Nucleic Acids Electrophoresis - Euroclone group€¦ · Nucleic Acids Electrophoresis 3 17...

Page 1: 3. Nucleic Acids Electrophoresis - Euroclone group€¦ · Nucleic Acids Electrophoresis 3 17 GellyPhor® HR GellyPhor® HR is a molecular biology grade standard melting temperature

3. Nucleic Acids Electrophoresis••••

AgarosesAcrylamidesBuffers & Reagents for DNA ElectrophoresisDNA LaddersHorizontal Electrophoresis System

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AgarosesEuroClone offers a complete selection of high quality agaroses for standard and specific electrophoresis appications.

GellyPhor® LE GellyPhor® LM GellyPhor® ULTRA GellyPhor® HR GellyPhor® PFGE

DNA separation 1-50 kb - - - - ✓

DNA/RNA separation ≤ 1 kb - - ✓ ✓ -

DNA/RNA separation ≥1 kb ✓ ✓ - . ✓

DNA separation 20-800 bp - - ✓ - -

Blotting ✓ - - ✓ ✓

DNA finger printing ✓ - - - -

Nucleic Acid Recovery ✓ ✓ ✓ ✓ ✓

Cat. Description FormatEMR010100 GellyPhor® LE 100 gEMR010500 GellyPhor® LE 500 gEMR010001 GellyPhor® LE 1 kg

StorageandStabilityFive years at Room TemperatureShippingRoom Temperature

GellyPhor®LM

GellyPhor® LM is a low melting temperature agarose. This molecular biology grade agarose produces gels with greater sieving properties and higher clarity than standard melting temperature agarose. The low melting temperature of GellyPhor® LM makes it ideal for preparative nucleic acids electrophoresis, while its low geling temperature is perfect for cloning of tissue culture cells and viral plaque assays.

TechnicalSpecificationGelling Temperature (dynamic measurement in 1.5% solution)

24-28 °C

Gel strength (1,5%) ≥ 500 g/cm2

Electroendosmosis (-mr) ≤ 0.12Sulphate ≤ 0.12%Moisture ≤ 7%Melting Temperature (1.5%) ≤ 65.5 °CRNase/DNase Activity none detected

GellyPhor®LEGellyPhor® LEis ideal for general nucleic acids preparative and analytical gel electrophoresis as well as for blotting. This gel sharply resolves the different DNA fragments to be analyzed and provides consistent resolution from batch-to-batch. It forms high strength gels with low background upon ethidium bromide staining. Due to the low electroendosmosis value (EEO) of GellyPhor® LE the DNA has a high electrophoretic mobility allowing shorter running time.

TechnicalSpecificationGelling Temperature (dynamic measurement in 1.5% solution)

34-38 °C

Gel strength (1%) ≥1200 g/cm2

Electroendosmosis (-mr) ≤ 0.12

Sulphate ≤ 0.14%

Moisture ≤ 8.5%

Residue on ignition ≤ 0.60%

RNase/DNase Activitynone detected

Cat. Description FormatEMR911100 GellyPhor® LM 100 g

StorageandStabilityFive years at Room TemperatureShippingRoom Temperature

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GellyPhor®HR

GellyPhor® HR is a molecular biology grade standard melting temperature agarose, that yields strong gels for fine resolution of small nucleic acids fragments. Performance testing of GellyPhor® HR ensures fine resolution of DNA fragments up to 1000 bp, though this agarose is capable of finely resolving DNA fragments ranging from 10 bp up to 1200 bp. GellyPhor® HR is designed for analytical electrophoresis.

TechnicalSpecificationGelling Temperature (dynamic measurement in 3% solution)

35.5 °C

Gel strength

≥ 600 g/cm2 (for a 1.5% gel)≥ 1500 g/cm2 (for a 3% gel)

Electroendosmosis (-mr) ≤ 0.12Sulphate ≤ 0.11%Moisture ≤ 7%Melting Temperature (3%) ≤ 80 °CRNase/DNase Activity none detected

Ranges of separation:1.8% 400-1200 bp3% 150-800 bp4.5% 15-400 bp

These ranges are approximate and have been calculated in TAE Buffer. To achieve the best resolution of GellyPhor® HR gels, they should be stored at 4 °C- 8 °C for 30 minutes before use.

Cat. Description FormatEMR912100 GellyPhor® HR 100 g

StorageandStabilityFive years at Room TemperatureShippingRoom Temperature

GellyPhor®ULTRA

GellyPhor® ULTRA is an intermediate melting temperature agarose that provides twice the resolution capabilities of the finest sieving agarose products. You can resolve DNA fragments, PCR and RT-PCR products differing in size by 2%, in the range of 200 bp to 800 bp, by horizontal gel electrophoresis (for example, a 200 bp DNA fragment can be separated from a 204 bp fragment). Using fast run protocols, DNA fragments differing in size by 1% can be resolved in as little as 1.5 hours in a 20 cm long horizontal or vertical gel format. GellyPhor® ULTRA agarose gels (2% to 4%) approximate the resolution of polyacrylamide gels (4% to 8%).

TechnicalSpecificationGelling Temperature (dynamic measurement in 3% solution)

≤ 36 °C

Gel strength (3%) ≥ 400 g/cm2 Electroendosmosis (-mr) 0.02 – 0.05Moisture ≤ 10%Melting Temperature (3%) ≤ 75 °CRNase/DNase Activity none detected

Cat. Description FormatEMR915100 GellyPhor® ULTRA 125 g

StorageandStabilityFive years at Room TemperatureShippingRoom Temperature

GellyPhor®PFGE

GellyPhor® PFGE is a very high gel strength, low EEO, standard gelling temperature agarose. Due to its high gel strength, this agarose can be used for the preparation of low percentage gels for the analysis not only of high molecular weight nucleic acids (such as chromosomes), but also of large-sized particles such as viruses and ribosomes.

TechnicalSpecificationGelling Temperature (dynamic measurement in 1.5% solution)

36 ± 1.5 °C

Gel strength≥ 1600 g/cm2 (for a 1% gel)≥ 3000 g/cm2 (for a 1.5% gel)

Electroendosmosis (-mr) 0.13Moisture ≤ 7%Melting Temperature (1.5%) ≤ 1.5 °CRNase/DNase Activity none detected

Cat. Description FormatEMR916100 GellyPhor® PFGE 100 g

StorageandStabilityFive years at Room TemperatureShippingRoom Temperature

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AcrylamidesFor the separation of oligonucleotides with a small number of bases, acrylamide gels with a small pore size are used. The pore size is determined by the total concentration of acrylamide and the ratio of Acrylamide/Bisacrylamide. For the sequencing of nucleic acids a ratio of Acrylamide/Bisacrylamide 19:1 is used. EuroClone offers a ready-to-use 40% Acrylamide/Bisacrylamide 19:1 mix.

Cat. Description FormatEMR013500 Acrylamide/Bisacrylamide 19:1 (solution 40%) 500 mlEMR013001 Acrylamide/Bisacrylamide 19:1 (solution 40%) 1 L

EuroSafe–NucleicAcidStain

EuroSafe Nucleic Acid Staining Solution (20,000x) is a safe nucleic acid stain, an alternative to the traditional ethidium bromide (EtBr) stain for detecting nucleic acid in agarose gels. It emits green fluorescence when bound to DNA or RNA. This stain has two fluorescence excitation maxima when bound to nucleic acid, one centered at 309 nm and another at 419 nm. In addition, it has one visible excitation at 514 nm. The fluorescence emission of EuroSafe bound to DNA is centered at 537 nm. EuroSafe is as sensitive as EtBr. Compared to EtBr, known as a strong mutagen it causes much fewer mutations in the Ames test. In addition, EuroSafe Nucleic Acid Staining Solution (20,000x) has a negative result in mouse marrow chromophilous erythrocyte micronucleus test and mouse spermary spermatocyte chromosomal aberration test.

Buffers and reagents for DNA electrophoresis

FeaturesUsed for detecting DNA and RNA Alternative to the ethidium bromide staining As sensitive as EtBr or more sensitive than that Less mutagenic than EtBr

Cat. Description FormatEMR440001 EuroSafe 1 ml

StorageandStabilityTwo years at Room TemperatureShippingRoom Temperature

Cat. Description Format Cat. Description FormatEMR100001 Boric acid 1 kg EMR224001 TBE Buffer, 10X - MBG 1 LEMR100005 Boric acid 5 kg EMR224500 TBE Buffer, 10X - MBG 500 mlEMR130001 EDTA disodium salt dihydrate 1 kg EMR042001 TBE Buffer, 10X - powder 1 LEMR130500 EDTA disodium salt dihydrate 500 g EMR227001 TE Buffer pH 8.0, 1X 1 LEMR034001 EDTA - Solution pH 8.0 (0,5 M) 1 L EMR226001 TE Buffer pH8.0, 100X 1 LEMR034500 EDTA - Solution pH 8.0 (0,5 M) 500 ml EMR226500 TE Buffer pH8.0, 100X 500 mlEMR036015 Ethidium Bromide 0,07% 15 ml EMR045001 Tris 1 kgEMR035025 Ethidium Bromide 1% 25 ml EMR045500 Tris 500 gEMR063001 TAE Buffer 10X 1 L EMR270001 Tris-HCl 1 kgEMR064001 TAE Buffer 50X 1 L EMR270500 Tris-HCl 500 gEMR427005 TBE Buffer, 10X 5 L

For the complete list of EuroClone Biochemicals please refer to Chapter 7 or visit

www.euroclone.it

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DNA LaddersEuroClone has three different DNA Ladders for sizing and approximate quantification of double-stranded DNA fragments such us PCR products. The Ladders have extremely sharp bands and reference bands with higher DNA content for easy orientation.

SHARPMASS™50-DNALADDER

This 50 bp DNA-Ladder is composed of 13 chromatography-purified individual DNA ranging from 50 to 1000 bp.The 500 bp and 250 bp fragments have a higher DNA content and serve as size references.

SHARPMASS™100-DNALADDER

This Ladder includes many plasmids fully digested by restriction enzymes. This process leads to the creation of 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging from 100 to 1000 bp. The 500 bp fragment has enhanced brightness and can be used as reference point.

SHARPMASS™100PLUS-READY-TO-LOADDNALADDER

This Ladder consists of 12 DNA fragments ranging from 100 bp to 3 kb. It is designed to show virtually uniform spacing over a wide fragment range. The Ladder allows sizing and concentration estimate of DNA fragments on agarose gels generated by PCR or restriction digest. The fragment mix is supplied in ready-to-use format containing orange and blue tracking dyes.

SHARPMASS™1-DNALADDER

This Ladder includes many plasmids fully digested by restriction enzymes. This process leads to the creation of 13 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging from 250 to 10000 bp. For an easier reference, the fragment of 1000 bp generates a three times brighter band and the 3.000 bp generates a two times brighter band.

Cat. Description FormatEMR810050 SHARPMASS™ 50 50 μgEMR810250 SHARPMASS™ 50 250 μgEMR811050 SHARPMASS™ 100 50 μgEMR811250 SHARPMASS™ 100 250 μgEMR813100 SHARPMASS™ 100 PLUS 100 lanesEMR812050 SHARPMASS™ 1 50 μgEMR812250 SHARPMASS™ 1 250 μg

StorageandStabilityOne year at -20 °CShippingBlue Ice

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Horizontal Electrophoresis Systems

Minigel Systems

EuroClone minigel systems are ideal for use with low to medium sample numbers. Designed for a quick and reproducible separations, these minigel systems are perfectly suited for the daily needs of the research laboratory.

SystemComponents• UV-Transmissible (UVT) Gel Tray with two Comb position• Two Combs

ConvenientHandling• Easy-to-use casting systems - gel directlly in the chamber by 90 degree rotation of the rubbers-seal-equipped gel tray• Labeled Combs for easy identification of Comb type• Venting slot prevents water condensation for clear gel viewing• Gel tray and chamber lid are made of UV-Transmissible acrylic glass enabling the gel to be viewed under UV excitation

AccessoriesA wide range of Combs, UV-transmissible gel trays featuring various Comb positions, multi casting rails for pouring several gels simultaneously, adjustable universal casting rail to accomodate gel trays of various sizes are available to complement this range.

MiniS

FeaturesGel Size 7 x 8 cmFootprint 12 x 9.5 x 16 cmSample Flexibility max. 24Buffer Volume 400 mlGel Volume 30 ml

Cat. DescritpionEM40-0708 Mini S Horizontal

The system Mini S includes:

• Buffer Chamber with Platinum Electrodes• SuperSafe Lid with Attached Leads• Gasketed UV-Transmissible (UVT) Gel Tray• 2 Combs: 1.5 mm thickness (6,10 teeth)

AccessoriesCat. DescritpionEM40-0708-10C 10 tooth Comb, 1.0 mm thick, Sample volume: 18 µlEM40-0708-10D 10 tooth Comb, 1.2 mm thick, Sample volume: 26 µlEM40-0708-12C 12 tooth Comb, 1.0 mm thick, Sample volume: 14 µlEM40-0708-12D 12 tooth Comb, 1.5 mm thick, Sample volume: 21 µlEM40-0708-5C 5 tooth Combs, 1.0 mm thick, Sample volume: 42 µlEM40-0708-5D 5 tooth Combs, 1.5 mm thick, Sample volume: 64 µlEM40-0708-6C 6 tooth Comb, 1.0 mm thick, Sample volume: 34 µlEM40-0708-6D 6 tooth Comb, 1.5 mm thick, Sample volume: 51 µlEM40-0708-8C 8 tooth Comb, 1.0 mm thick, Sample volume: 24 µlEM40-0708-8D 8 tooth Comb, 1.5 mm thick, Sample volume: 36 µlEM40-0708-PD 2 tooth, preparative Comb, 1.5 mm thick

EM40-0708-UVTRapidCast UVT Gel Tray 7 x 8 cm with two Comb slots & gasketed ends

EM40-0708-CST RapidCast Gel Caster 7 x 8 cm for use with 80-0708-RCGT Allows for three gels to be cast simultaneously. CASTER ONLY

EM40-0708-GK Gasket, 80-0708-RCGT, pk 2

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MiniM

FeaturesGel Size 9 x 11 cmFootprint 15 x 9.5 x 22 cmSample Flexibility max. 36Buffer Volume 600 mlGel Volume 50 ml

Cat. DescriptionEM40-0911 Mini M Horizontal

System Components:• Buffer Chamber with Platinum Electrodes• SuperSafe Lid with Attached Leads• Gasketed UV-Transmissible (UVT) Gel Tray• 2 Combs: 1.5 mm thick (10, 14 teeth)

AccessoriesCat. DescriptionEM40-0911-10C 10 tooth Comb, 1.0 mm thick, Sample volume: 25 µlEM40-0911-10D 10 tooth Comb, 1.5 mm thick, Sample volume: 38 µlEM40-0911-12C 12 tooth Comb, 1.0 mm thick, Sample volume: 20 µlEM40-0911-12D 12 tooth Comb, 1.5 mm thick, Sample volume: 30 µlEM40-0911-14C 14 tooth Comb, 1.0 mm thick, Sample volume: 16 µlEM40-0911-14D 14 tooth Comb, 1.5 mm thick, Sample volume: 25 µlEM40-0911-5C 5 tooth Comb, 1.0 mm thick, Sample volume: 58 µlEM40-0911-5D 5 tooth Comb, 1.5 mm thick, Sample volume: 86 µlEM40-0911-8C 8 tooth Comb, 1.0 mm thick, Sample volume: 34 µlEM40-0911-8D 8 tooth Comb, 1.5 mm thick, Sample volume: 51 µl

EM40-0911-18C18 tooth Comb, 2 x (8+1), 2 x microtiter format, 1.0 mm thick, Sample volume: 11 µl

EM40-0911-18D18 tooth Comb, 2 x (8+1), 2 x microtiter format, 1.5 mm thick, Sample volume: 16 µl

EM40-0911-9C9 tooth Comb, (8+1), 1 x microtiter format, 1.0 mm thick

EM40-0911-9D9 tooth Comb, (8+1), 1 x microtiter format, 1.5 mm thick

EM40-0911-PD 2 tooth, preparative Comb, 1.5 mm thick EM40-0911-UVT RapidCast UVT Gel Tray with two Comb slots

EM40-0911-CST

RapidCast Gel Caster, 9 x 11 cm for use with 80-0911-RCGTAllows casting of three gels simultaneously. CASTER ONLY

EM40-0911-GK Gasket, for RapidCast Gel Tray 80-0911-RCGT, pk 2

MiniL

FeaturesGel Size 12 x 14 cmFootprint 17 x 9.5 x 25 cmSample Flexibility max. 50Buffer Volume 800 mlGel Volume 85 ml

Cat. DescriptionEM40-1214 Mini L Horizontal

System Components:• Buffer Chamber with Platinum Electrodes• SuperSafe Lid with Attached Leads• Gasketed UV-Transmissible (UVT) Gel Tray• 2 Combs: 1.5 mm thick (12, 20 teeth)

AccessoriesCat. DescriptionEM40-1214-16C 16 tooth Comb, 1.0 mm thick, Sample volume: 20 µlEM40-1214-16D 16 tooth Comb, 1.5 mm thick, Sample volume: 30 µlEM40-1214-20C 20 tooth Comb, 1.0 mm thick, Sample volume: 15 µlEM40-1214-20D 20 tooth Comb, 1.5 mm thick, Sample volume: 22 µlEM40-1214-24C 24 tooth Comb, 1.0 mm thick, Sample volume: 11 µlEM40-1214-24D 24 tooth Comb, 1.5 mm thick, Sample volume: 17 µlEM40-1214-8C 8 tooth Comb, 1.0 mm thick, Sample volume: 47 µlEM40-1214-8D 8 tooth Comb, 1.5 mm thick, Sample volume: 70 µl

EM40-1214-12C12 tooth Comb, 1 x microtiter format, 1.0 mm thick, Sample volume: 27 µl

EM40-1214-12D12 tooth Comb, 1 x microtiter format, 1.5 mm thick, Sample volume: 35 µl

EM40-1214-25C25 tooth (12x2)+1), 2x microtiter format, 1.5 mm thick, Sample volume: 11 µl

EM40-1214-25D25 tooth (12x2)+1), 2x microtiter format, 1.0 mm thick, Sample volume: 16 µl

EM40-1214-9C8+1 tooth Comb, 1 x microtiter format, 1.0 mm thick, Sample volume: 27 µl

EM40-1214-9D8+1 tooth Comb, 1 x microtiter format, 1.5 mm thick, Sample volume: 40 µl

EM40-1214-PD2 well, Preparative Comb, 1.5 mm thick, Sample volume: 596/28 µl

EM40-1214-WC Wall Comb, 1.5 mm thick EM40-1214-GK Gasket, for RapidCast UVT Tray 80-1214-RCGT, pk. 2

EM40-1214-UVT12RapidCast UVT Gel Tray, 12 x 14 for 80-1214 with twelve Comb slots

EM40-1214-UVT4RapidCast UVT Gel Tray, 12 x 14 for 80-1214 with four Comb slots

EM40-1214-CST

RapidCast Gel Caster 12 x 14 cm for use with 80-1214-RCGTAllows for three gels to be cast simultaneously. CASTER ONLY

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MiniExM

FeaturesGel Size 14.4 x 10.2 cmFootprint 19 x 9.5 x 20 cmSample Flexibility max. 80Buffer Volume 600 mlGel Volume 70 ml

Cat. DescriptionEM40-1410 Mini ExM Horizontal

System Components:• Casting Chamber• Buffer Chamber with Platinum Electrodes• SuperSafe Lid with Attached Leads• Gasketed UV-Transmissible (UVT) Gel Tray• 4 Combs: 1.5 mm thickness (2 x 30, 2 x 40 teeth)

Accessories:A wide range of Combs (compatible with multichannel pipettors) are available. Please contact EuroClone for the complete list of accessories.

MiniExW

FeaturesGel Size 23 x 14 cmFootprint 30 x 10.5 x 24 cmSample Flexibility max. 200Buffer Volume 800 mlGel Volume 160 ml

Cat. DescriptionEM40-2314 Mini ExW Horizontal

System Components:• Buffer Chamber with Platinum Electrodes• SuperSafe with Attached Leads, Casting Chamber• Gasketed UV-Transmissible (UVT) Gel Tray• 4 Combs: 1.5 mm thickness (50 teeth microtiter format)

Accessories:A wide range of Combs (compatible with multichannel pipettors) are available. Please contact EuroClone for the complete list of accessories. Maxi L system allows 40 cm long runs).

Maxigel Systems

Maxi Gel Systems are very high quality electrophoresis systems designed for medium to high sample throughput (up to 600 samples can be run simultaneously) and for high-resolution nucleic acid separations requiring long migration paths (the Maxi L system allows 40 cm long runs).

MaxiS

FeaturesGel Size 13 x 25 cmFootprint 10 x 21.5 x 37.5 cmSample Flexibility max. 112Buffer Volume 1600 mlGel Volume 160 ml

Cat. DescriptionEM41-1325 Maxi S Horizontal

System Components:• Buffer Chamber with Platinum Electrodes• SuperSafe Lid with Attached Leads• UV-Transmissible (UVT) Gel Tray with Endgates• Three Point Leveling Base• 3 Combs: 1.5 mm thickness (12, 16, 20 teeth)

Accessories:A wide range of Combs (compatible with multichannel pipettors) are available. Please contact EuroClone for the complete list of accessories.

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*A wide range of Combs (compatible with multichannel pipettors) are available. Please contact EuroClone for the complete list of accessories.

MaxiM

FeaturesGel Size 20 x 25 cmFootprint 29 x 10 x 37 cmSample Flexibility max. 168Buffer Volume max. 2300 mlGel Volume 250 ml

Cat. DescriptionEM41-2025 Maxi M Horizontal

System Components:• Buffer Chamber with Platinum Electrodes• SuperSafe Lid with Attached Leads• UV-Transmissible (UVT) Gel Tray with Endgates• Three Point Leveling Base• 3 Combs: 1.5 mm thickness (16, 24, 36 teeth)

Accessories:Cat. Description

EM41-2025-BPLong Horizontal Gel Box, 20 x 25 cm with integral leveling platform & Buffer Exchange Ports;ExpressCast Gel Tray with six Comb slots and three Combs

EM41-2025-EG End gates for ExpressCast UVT Gel Tray 81-2025-EXGT, pk 2 includes gasketsEM41-2025-GK Gasket, for ExpressCast UVT Gel Tray 81-2025-EXGT, pk 2

EM41-2025-UVTExpressCast UVT Gel Tray 20 x 25 cm with gasketed end gates and six Comb slots that allows for 1, 2, 3 or 4 gels of equal length to be run in the tray

EM41-2025-12C 12 tooth Comb 1.0 mm thick, Sample volume: 54 µlEM41-2025-12D 12 tooth Comb 1.5 mm thick, Sample volume: 82 µlEM41-2025-16C 16 tooth Comb 1.0 mm thick, Sample volume: 39 µlEM41-2025-16D 16 tooth Comb 1.5 mm thick, Sample volume: 59 µlEM41-2025-20C 20 tooth Comb, 1.0 mm thick, Sample volume: 30 µlEM41-2025-20D 20 tooth Comb, 1.5 mm thick, Sample volume: 45 µlEM41-2025-24C 24 tooth Comb, 1.0 mm thick, Sample volume: 24 µlEM41-2025-24D 24 tooth Comb, 1.5 mm thick, Sample volume: 37 µlEM41-2025-28C 28 tooth Comb, 1.0 mm thick, Sample volume: 19 µlEM41-2025-28D 28 tooth Comb, 1.5 mm thick, Sample volume: 28 µlEM41-2025-32C 32 tooth Comb, 1.0 mm thick, Sample volume: 15 µlEM41-2025-32D 32 tooth Comb, 1.5 mm thick, Sample volume: 22 µlEM41-2025-36C 36 tooth Comb, 1.0 mm thick, Sample volume: 13 µlEM41-2025-36D 36 tooth Comb, 1.5 mm thick, Sample volume: 20 µlEM41-2025-8C 8 tooth Comb, 1.0 mm thick, Sample volume: 85 µlEM41-2025-8D 8 tooth Comb, 1.5 mm thick, Sample volume: 127 µlEM41-2025-18C 18 tooth (16 sample lanes & 2 marker lanes) 1 x microtiter Comb, 1.0 mm thick, Sample volume: 27 µlEM41-2025-18D 18 tooth (16 sample lanes & 2 marker lanes) 1 x microtiter Comb 1.5 mm thick, Sample volume: 40 µlEM41-2025-MTC 21 tooth 1 x microtiter Comb, 1.0 mm thick, Sample volume: 27 µlEM41-2025-MTD 21 tooth 1 x microtiter Comb, 1.5 mm thick, Sample volume: 40 µlEM41-2025-MT2C 42 tooth 2 x microtiter Comb, 1.0 mm thick, Sample volume: 11 µlEM41-2025-MT2D 42 tooth 2 x microtiter Comb, 1.5 mm thick, Sample volume: 16 µlEM41-2025-PD Preparative Comb, 1.5 mm thick, Sample volume: 1052/28 µlEM41-2025-WC Wall Comb, 1.5 mm thick

MaxiLFeatures

Gel Size 23 x 40 cmFootprint 32 x 10 x 53 cmSample Flexibility max. 600Buffer Volume 4500 mlGel Volumne 460 ml

Cat. DescriptionEM41-2340 Maxi L Horizontal

System Components:• Buffer Chamber with Platinum Electrodes• SuperSafe with Attached Leads• UV-Transmissible (UVT) Gel Tray with Endgates• Three Point Leveling Base• 4 Combs: 1.5 mm thickness (2 x 25, 2 x 50 teeth)Accessories*

MaxiExW Features

Gel Size 23 x 25 cmFootprint (W x H x L) 30 x 11 x 37 cmSample Flexibility max. 500Buffer Capacity max. 3000 mlGel Volume 160 ml

Cat. DescriptionEM41-2325 Maxi ExW Horizontal

System Components:• Buffer Chamber• SuperSafe Lid with Attached Leads• UV-Transmissible (UVT) Gel Tray with Endgates• Three Point Leveling Base• 4 Combs: 1.5 mm thickness (2 x 25, 2 x 50 teeth)

Accessories*

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