140627 SMK Penang Free Talk
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Transcript of 140627 SMK Penang Free Talk
The extraction and separation of
chlorophyll (α+β) carotin and
xanthophyll in fresh green leaves
Eugene Ong | [email protected] Institute for Research in Molecular Medicine Universiti Sains Malaysia
27 June 2014 SMK Penang Free
Thin-Layer Chromatography
lutein (yellow) chlorophyll a (yellow – green)
chlorophyll b (dark green)
β-carotene (yellow)
pheophytin (brown – green)
xanthophylls
STATIONARY PHASE Plate coated with silica.
LEAF EXTRACT
MOBILE PHASE Solvents (acetone, alcohol, hexane, etc)
x y Retention value,
Rf = x y
Thin-Layer Chromatography
STATIONARY PHASE Plate coated with silica.
LEAF EXTRACT
MOBILE PHASE Solvents (acetone, alcohol, hexane, etc)
1938, Ukraine
Acetone, 1610, France
Chlorophyll, 1817, France
Thin-Layer Chromatography
SCIENCE & TECHNOLOGY
Anything useful that we make is technology.
– K e v i n K e l l y
news.discovery.com/animals/animal-tool-use-111205.htm
The Horse In Motion (1878) The First Motion Picture Ever Made
Transformers: Age of Extinction (2014)
commons.wikimedia.org/wiki/User:Waugsberg, giphy.com, sirverdoso.es/lo-mas-visto-del-ano-2013
Star Wars (1977)
1865 – Gregor Mendel introduced principles of basic genetics.
unlockinglifescode.org
1977 – Frederick Sanger developed rapid DNA sequencing technique.
1953 – James Watson and Francis Crick discovered the structure of DNA.
1990-2003 HUMAN GENOME SEQUENCING PROJECT $1 billion Multinational effort (US, Japan, Europe)
2014 Today: $1000
An analysis of the history of technology shows that technological change is exponential.
– R a y K u r z w e i l
T h e
13.8 billion years
438 years per second, 1.58 million years per hour, and 37.8 million years per day. Cosmos: A Spacetime Odyssey
Knowledge is of two kinds.
We know a subject ourselves,
or we know where we can find
information on it.
– S a m u e l J o h n s o n