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Humoral and Cellular Response Against Native and Synthetic Antigens.

Institute of Maritime and Tropical Medicine

Department of Immunology & National Salmonella Centre

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Department of Immunology & National Salmonella Centre

Human Resources:

- Full professor: Renata Głośnicka MD, Ph.D.

- Senior scientists: Danuta Kunikowska Ph.D.

Bożena Dera-Tomaszewska Ph.D.

Jan Wysocki MD, Ph.D.

- Scientists: Halina Dziadziuszko M.Sc.

Ewa Tokarska-Pietrzak M.Sc.

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OUR TEAM:

From centre to left (clockwise):

Prof. Renata Głośnicka, Dr. Bożena Dera-Tomaszewska, Jolanta Zabłocka, Dorota Kenig, M.Sc. Halina Dziadziuszko, Gabriela Nawrot, M.Sc. Ewa Tokarska -Pietrzak, Dr. Danuta Kunikowska

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PREVIOUS RESEARCH Immunochemistry research:

- Salmonella bacteria antigens

- Lipopolysaccharides

- Flagella

- Vi antigen

- Yersinia pestis envelope antigen

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Immunochemistry Research

Lipolysaccharide of Salmonella Haarlem (9.46) – detection the new OPS composition: 3,9,46 (according to Kauffmann-White scheme).

Epitope analysis of S. Enteritidis isolated flagella antigen and synthetic peptides of hypervariable part of this antigen.

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Immunological investigations of viruses synthetic peptides.

Hepatitis B Virus:

- X-Gene coded protein fragments;

- surface antigen (140-146) region fragment. Hepatitis C Virus:

- protein fragments

(evaluation of their immunogenicity and

diagnostic propertis in HCV infection)

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Immunological investigations of heat shock protein fragments.

Human hsp 70 fragment (29-42), and

their cyclic analogue – conformational studies and evaluation of its immunogenicity.

Human hsp 60 fragment (409-424) – is recognized by serum antibodies of patients with acute coronary syndroms.

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Immunological investigations of heat shock protein.

Characterisation of the anti-DnaJ monoclonal antibodies and their use to compare immunological properties of DnaJ and its human homologue HDJ-1 – epitope analysis of the similar domains of E. coli DnaJ and human HDJ-1 (in cooperation with Prof. B. Lipińska team, UG).

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CURRENT RESEARCH

National Salmonella Centre

- diagnostics of Salmonella strains isolated

by field laboratories

- molecular research of Salmonella strains

- epidemiological research within WHO „Salmonella Surveillance” project

1012.04.23

Salmonella infections among people in the years 1971-2000

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CULTURE COLLECTIONS

• Salmonella strains ( KOS Collection - about 2000 strains)

• Bacteriophage preparations

• Mouse myeloma cell lines

• Mouse macrophages cell line

• Mouse hybridoma cell lines producing monoclonal antibodies

anti Salmonella antigens and anti DnaJ from E.coli.

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POLYVALENT VACCINE „IMMUNOVAC” AGAINST SALMONELLOSIS IN ANIMALS

„Immunovac” vaccine is composed of several Salmonella serovars and polyclonal antibodies

Generates active and passive immunization after oral administration.

The vaccine is in the course of commercialization by BIOWET in Puławy.

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IMMUNOLOGICAL INVESTIGATIONS OF PORPHYRIN DERIVATIVES

Porphyrines and their amino acids derivatives • PP(FAla)2Arg2• PP(Ala)2Arg2• PP(Ser)2Arg2 and their mixture (Sensifirine)are intended to be used in PDT (photodynamic therapy).Our goal is to investigate their impact on the immune system. The researches are performed in cooperation with the teams of prof. Alfreda Graczyk (MUT, Warsaw) and prof. Anna Podhajska ( MBW AMG-UG, Gdańsk)

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YANTIGENS

ANTIBODIES &

HOW DO THEY WORK ?

MOLECULES MEDICINE

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THANK YOU

FOR YOUR ATTENTION