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Transcript of 1 PHM142 UNIT 9A Mitochondrial Diseases and the Brain Instructions and materials: Read ONLY sections...
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PHM142 UNIT 9A
Mitochondrial Diseases and the Brain
Instructions and materials:
Read ONLY sections in paper by Chaturvedi and Beal, 2013 (included in lecture material) highlighted in yellow, plus Figures 1 and 3 and Table 1 in this paper.
Be prepared to discuss these topics in class.
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PHM142 UNIT 9BMitochondrial Function and hepatic
detoxification of a) monoamines, alcohol, toluene
b) heme & bilirubin
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a) Detoxification of monoamines catalysed by mitochondrial outer membrane MAO
R-CHNH2
O2
H2O2
R-CH=NH
NH3
R-CHO
R-COOH-oxidation
CO2
ATP, HCO3
-
UREAurea cycle
MONOAMINE OXIDASEFlavin-containing amine oxidase
(imine)
inner membrane
outer membrane
e.g.,dopaminenorepinephrinetyraminephenethylamineoctylamineserotonin
aldehyde dehydrogenase
NAD+
NADH
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Amine specificity for the two isoforms (A & B) in humansMAO A preferentially metabolizes serotonin.MAO B preferentially metabolizes phenethylamine, dopamine.
MAO inhibitor + dietary amines or proprietary drugs
MAOB inhibitor deprenyl (selegiline) similar to phenethylamine and increases brain dopamine levels. This is used to treat Parkinson’s disease.
But “Hypertensive crisis” is a hyperadrenergic state induced by MAO inhibitors + pressor amines (e.g., tyramine in cheese,beer,wine or soya sauce) or proprietary drugs (e.g., L-DOPA, ephedrine, etc).
Cheese Tyramine (g/g)
English Stilton 1157
Blue 998
Mozzarella 158
Feta 76
Processed cheese slice nil
Cheese Tyramine (g/g)
Cheddar cheese, old 1530
Beer 2-11
Sherry Wine 3
Chianti Wine 25
Perry, 1996. http://www.vh.org/adult/provider/psychiatry/CPS/19.html
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b) Detoxification of alcohols by matrix ALDH2
ETHANOL acetaldehyde
cytosolic alcoholdehydrogenase
NAD+NADHMethylpyrazole
Covalently binds to protein-NH2
NAD+
NADH
aldehydedehydrogenase
acetate ATP
CoASHacetylCoA
CO2
TCAcycle
ANTABUSE (disulfiram) or cyanamide
ADH ALDH Suscept. to alcoholism Appearance social drinking
Chromosome # 4 9, 12, 17
Caucasians 99% normal 90% normal 10% (>) --
Japanese 90% atypicial 40% deficient < 10% flushing
(ADH)
(ALDH)
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CONHCH2COOH
Hippuric acid
URINE
glycine
CH3 CH2OH CHO
COOH
Toluene Benzyl alcohol Benzaldehyde
Benzoic acid
ADH
ALDH1/ALDH2
Cyt P-450
Teratogen Update: Toluene teratology. 55, 145-51, (1997)
Benzoyl-CoA synthetase
ATPCoA
Benzoyl-CoA
N-acyltransferase
c) Benzoic acidosis induced by toluene glue sniffing
ER CYTOSOL
MITOCHONDRIA
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Cytochrome Heme Synthesis - Overall picture
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Heme synthesis
• Heme required for synthesis of mitochondrial cytochromes and endoplasmic reticular P450s.
• Heme required for bone marrow synthesis of hemoglobin and muscle myoglobin
• STEP 1 for heme synthesis is the synthesis of aminolevulinic acid (ALA) from succinyl CoA of the citric acid cycle and glycine
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Heme biosynthesis in bone marrow (hemoglobin) and liver (cytochromes)
R.L.S. ALA synthetaseCOO
CH2
CH2
C
O
S CoA
Succinyl CoA
COO
CH2
COO
COO
CH2
CH2
C CH2
NH3
O
H+ CO2 + CoA
Feedback inhibition by heme(also induces heme oxygenase) Aminolevulinate
(ALA)
Glycine
HEME BIOSYNTHESISSTEP II
(CYTOSOL)
Fe, O2
COO
CH2
CH2
C CH
O
O
4,5-dioxovaleric acid (DOVA)
1) Brain Neuropsychiatric problems in acute porphyria, 2) Liver necrosis / cancer particularly if Fe overload
1) HEME arginate2) High carbohydrate
+
glucose-6-P NADPH
GSH reductaseGSH peroxidase
H2O2
-ketoglutaratesuccinate
citricacidcycle
CO2 + CoA
dehydrogenasecomplex
Therefore ANAPLEROTICreaction required to replacesuccinyl CoA in citric acidcycle.
ALA synthetase induced by:1) Heme deficiency due to excess P450 synthesis/induction e.g., by barbiturates Sulfonamides: Therefore an increase in urine porphyrins2) ERYTHROPOIETIN (formed by kidneys) improves quality of life of patients on kidney dialysis.3) Acute intermittent porphyria or ALA dehydratase deficiency.4) LEAD
Mitochondrial Matrix
pyridoxal
-ketoglutarateALA toxicity
Arch Biochem Biophys. 373, 368-74, (2000)
ALA SYNTHESIS AND TOXICITY
ALA accumulatesa) Acute intermittent porphyriab) Lead
NH4+, O2
ROS
DNA strand breaks
DNA ADDUCTSLIVER CANCER
Therapy
1
or leadNH2
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Erythropoietin synthesised by kidney induces hemoglobin synthesis in bone marrow (replaces blood transfusion!)
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Heme Biosynthesis - STEP 2 in the cytosol (4 enzymes 2-5)
NH HN
NH HN
COOH
COOH
COOH
COOH
HOOC
HOOC
HOOC
coproporphyrinogen I
CYP1A2
NH HN
N HN
PA
PA
A
P P
A
NH HN
NH HN
HO
COOH
COOH
COOH
COOH
HOOC
HOOC
HOOC
non-enzymatic
COOH
COOH
NH
HOOC
HOOC NH2
COOH
H2N
O
NH HN
NH HN
COOH
COOH
COOH
HOOC
HOOC
HOOC
COOH
COOHUroporphyrinogen III synthase
NH HN
NH HN
COOH
COOH
COOHCOOH
CYP1A2
LIVER/BONE MARROW cytosol
ALA
Uroporphyrin
Coproporphyrinogen III
Uroporphoryrinogen decarboxylase
-4CO2
Uroporphyrinogen I
Symmetrical
Porphobilinogen (PBG)
PBGSYNTHASE
r.l.s polymorphism
Hydroxymethylbilane
ALA DehydrataseDeficiencyPorphryia *
phosphobilinogendeaminase
Acute IntermittentPorphyria*
CongenitalErythropoieticPorphyria
PorphyriaCutaneaTarda
PorphyriaCutaneaTarda
(UROPORPHYRIA)
Cutaneous Porphyria
* Acute Porphyria
2
3
4
5
5
STEP 1
STEP 3
1
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Heme Biosynthesis - STEP 3 - mitochondrial final steps (3 enzymes)
NH N
N HN
COOHCOOH
NH HN
NH HN
COOHCOOH
N N
N N
COOHCOOH
Fe2+
MITOCHONDRIA
O2 CO2 mitochondrialintermembrane space
Protoporphyrinogen IX
Protoporphyrinogen oxidase
i.m.
O2
Protoporphyrin IX
Ferrochelatase
i.m.r.l.s
HEME
Coproporphyrinogen oxidase
NH HN
NH HN
COOH
COOH
COOHCOOH
Coproporphyrinogen III
HereditaryCoproporphyria *
6
78
VariegatePorphyria *
Protoporphyria
Acute Porphyria
* Cutaneous Porphyria
STEP 2
Fe
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OXIDATIVE DEGRADATION OF HEME TO BILIRUBIN
(a brain neurotoxin in babies)
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Detoxification function of the livera) heme toxin oxidation to bilirubinb) detoxification of bilirubin by glucuronidation or albumin
phagosomeHemeoxygenase
albumin LIVER
CO
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Degradation of heme STAGE 1Hemoglobin of old erythrocyte trappedin spleen
NH
NH
N NH
CH
CH
CH
OO
NH
NH
NH
NH
CH
CH2
CH
OO
M V M P P M M V
VMMPPMVM
BiliverdinNADPH + H+
NADP+
Biliverdin Reductase
Excreted by Reptilesand birds
HEMEO2 + NADPH
H2O + NADP+
Fe3+
BILIRUBIN(Neurotoxin to babies)
Bone marrow
transferrin
Plasma Serum Albumin Complex LIVER
Modified fromFig. 28-31 Stryer4th Ed.
NEONATAL JAUNDICEBilirubin accumulates in newborns.(destroy with sunlight).
Heme oxygenase (ER)
CO
H2O soluble
exhaled
ANTIOXIDANT
Fat soluble
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STAGE II - ligandin in hepatocyte surface membrane traps bilirubinfrom plasma and helps transport it into the liver where it is glucuronidated
Albumin- bilirubin
(a GSH transferase)
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*mutated gene in Gilbert’s disease(carried by 15% ofpopulation)
NH
NH
NH
NH
CH
CH2
CH
OO
VMMMVM
COOCOO
OH
OH
H
UDP
H
OHH
OH
COO
UDP-glucuronate
+
Bilirubin
Endoplasmic reticulumUDP-glucuronosyltransferase (UGT 1A1*)
NH
NH
NH
NH
CH
CH2
CH
OO
VMMMVM
COOC
OH
OH
H
O
H
OHH
OH
COO
O
BILIRUBIN DIGLUCURONIDE (Soluble bilirubin diglucuronide secreted
into the bile)
BILIRUBIN MONOGLUCURONIDE
UDP
UDP gluc. + UGT 1A1