1 July 2009 1 CoolLEDs pE LED excitation systems.
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Transcript of 1 July 2009 1 CoolLEDs pE LED excitation systems.
1 July 2009www.coolled.com/life-sciences-analytical/
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CoolLED’s pE LED excitation systems
1 July 2009www.coolled.com/life-sciences-analytical/
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CoolLED
CoolLED is a UK-based specialist LED technology manufacturer
It is owned by Custom Interconnect Ltd. (CIL), an established electronics manufacturing company
CoolLED’s Life Sciences & Analytical division supplies the pE family of LED excitation products
CoolLED builds its LED products using the bare semiconductor LED die to achieve the highest possible performance.
CoolLED is not restricted to commercially available packaged LED products which means it can offer a greater range of wavelengths, stability and intensity
www.CoolLED.com
1 July 2009www.coolled.com/life-sciences-analytical/
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CoolLED pE-series of modular LED fluorescence excitation products with a choice from (currently) 18 wavelengths
pE-1 is a 3-wavelength system
pE-2 is a 4-wavelength system
LEDs are carried on interchangeable LAMs (LED Array Modules)
Manual Control Pod or Automation (e.g. MetaMorph)
Instant On/Off and 0-100% control in 1% steps
Stable, repeatable and homogeneous excitation
Can be used in conjunction with other light sources
Makes high speed Shutters and ND Filters redundant
Product Overview
1 July 2009www.coolled.com/life-sciences-analytical/
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CoolLED has the largest range of LED wavelengths for exciting fluorophores
LED Wavelengths
1 July 2009www.coolled.com/life-sciences-analytical/
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1. Largest range of LED wavelengths
2. LEDs actively-cooled for maximum intensity and stability
3. Lifetime measured in tens of thousands of “on” hours
4. Homogeneous excitation over FOV
5. LED Array Modules (LAMs) are interchangeable for maximum flexibility and upgrade
6. Repeatable, constant excitation during lifetime
7. No moving parts, no need to re-align
8. Fully integrated under NIS Elements + MetaMorph, MicroManager, etc.
9. Rapid Switching and Pulsing in under 300 ms
10.Cost Effective option as Shutters and ND filters redundant
Key Benefits
1 July 2009www.coolled.com/life-sciences-analytical/
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LED excitation
Narrow waveband Stable
Better results from less damaged samples
Reliable comparisons of quantitative measurements
Active-cooling of LEDs using a Peltier gives
best intensity and stability
Repeatable, reliable, and comparable results
Not exposing samples to unwanted wavelengths
e.g. UV
Less photo-bleaching
LED Excitation
1 July 2009www.coolled.com/life-sciences-analytical/
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LEDs are Controllable
Instant “On/Off”0-100% Intensity
in 1% steps
No warm up or cool down times
Optomise intensity independently foreach fluorophore
Intensities can be pre-set for a given test
Repeatable and consistent results
Switching does not affect LED lifetime
Fast switching between wavelengths
~300 uSecs with TTL
Perfect for pre-aligning samples on a Confocal
LEDs are controllable
1 July 2009www.coolled.com/life-sciences-analytical/
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Costs & Safety
Long lifetime measured in tens of thousands
of “On” hours
LEDs do not contain Mercury (Hg) which
is hazardous
No replacement lamps to purchase
No risk of explosion or H&S risk from Mercury vapour
International regulations are removing Mercury
from most products
No Hg disposal issues. No H&S regulations to
follow for operator
Switching does not affect LED lifetime
No need to re-align when replacing lamps
Costs & Safety
1 July 2009www.coolled.com/life-sciences-analytical/
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Better Results
Quantitative Live Cell
Meaningful comparisons can be made between measurements made
at different times
No vibration, no delay, no transfer of heat or
electromagnetic radiationto sample
A growing area for Fluorescence microscopy
as results can be more meaningful on living cells
Optimised intensity for each fluorophore. Dual staining applications do not require
an excitation filter wheel
CoolLED excitation is stable and repeatable due to active-cooling
Perfect for Time Lapse
Better Results with LEDs
1 July 2009www.coolled.com/life-sciences-analytical/
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Mercury - Metal Halide (contains Mercury) - Xenon
All suffer from the same drawbacks:-
• Short Lifetime
• Warm-up/cool-down period
Decreasing Intensity
• Use hazardous materials
• H&S / Disposal issues
• On-going cost of lamps
• Lamps need re-aligned
• Unstable
Traditional Lamps
1 July 2009www.coolled.com/life-sciences-analytical/
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Mercury LampsStrength Counter Argument
Broadband excitation Most needs to be filtered out
More intense Only at some wavelengths
Unwanted intensity can damage samples
Mercury Lamps
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Weakness Awareness Proposal
Warm-up / Cool-down period LEDs are almost instant
Declining intensity through life LED intensity remains for tens of thousands of hours
Lamps need to be replaced regularly
Virtually no running costs with LED systems
Lamps need re-aligned regularly CoolLED is aligned once-ever at installation
Lamps can explode Although rare, it is a serious H&S risk
Mercury Lamps
1 July 2009www.coolled.com/life-sciences-analytical/
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Strength Counter Argument
Broadband excitation Most needs to be filtered out
More intense Only at some wavelengths
Unwanted intensity can damage samples
Longer life than Mercury Still short by LED standards
Still declines during life
Metal Halide Lamps
1 July 2009www.coolled.com/life-sciences-analytical/
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Weakness Awareness Proposal
Warm-up / Cool-down period LEDs are almost instant
Declining intensity through life LED intensity remains for tens of thousands of hours
Lamps need to be replaced regularly
Virtually no running costs with LED systems
Metal Halide Lamps
1 July 2009www.coolled.com/life-sciences-analytical/
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Strength Counter Argument
Integrated with Axiovision Adds to investment cost as user requires latest version
Simultaneous excitation Often requested but most cameras are monochrome so fast switching is actually what is required. CoolLED is developing simultaneous in the future
Zeiss Colibri
1 July 2009www.coolled.com/life-sciences-analytical/
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Zeiss Colibri
Weakness Awareness Proposal
Expensive CoolLED pE-2 is ~€6K* List with 4λ * Based on current exchange from £5375
Intensity in Green is an issues IMP Vienna (Zeiss lab) reported that CoolLED is 4-5x intense in this region. Purchased 12 units
Only integrated with Axiovision Excludes users using NIS Elements, MetaMorph, MicroManager, etc.
Latest version of Axiovision required and adds to cost
Only 10 wavelengths available CoolLED has 18 and will develop more
1 July 2009www.coolled.com/life-sciences-analytical/
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Product Comment
Thor Labs Single wavelength
Lower intensity
No integration
Cairn Research Less wavelength options
Less intense
Fraen Less intense
Transmitted light option
Other LED sources
All other LED product suppliers are limited to using commercially available LED packaged parts which are not actively-cooled to achieve
CoolLED’s extra intensity and stability
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Summary
Use of Mercury-based lamps will decline
CoolLED LEDs offer your users many benefits including:-
• Instant On/Off
• Control of intensity from 0-100% in 1% steps
• No alignment or lamp replacement
In many instances, there can be an overall cost saving with a CoolLED system when shutters and ND filters are removed from microscope
You can contact CoolLED for advice and recommendations to ensure users get best performance
[email protected] www.CoolLED.com +44 (0) 1264 320989