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1 Corbett Research CAS-1200 Dimensions 490 (W) x 580 (D) x 390 (H) Operates from PC or laptop Manufactured and supported in Brisbane Slide 2 2 Slide 3 3 Pipetting Single pipetting head Pipette volumes as low as 1 L Repeatable to within 0.1 L 50 L and 200 L tips Filtered Conductive tips Slide 4 4 Level Sensing Level sensing is standard Requires conductive tips (black, carbon impregnated) Limits tip immersion More accurate pipetting Slide 5 5 Intuitive layout Multiple options to suit user Prepare master mix from reagents or add pre-prepared master mix Dilute Standards each time or use pre- prepared dilutions Normalize sample concentrations Import/Export of sample names (from CSV or tab delineated file or barcode) Wide range of plates can be used (384-, 96-, 72-, Gene-Disc etc) Software interface Slide 6 6 Compatible with 1.5 ml tapered tubes sample format Compatible with Corbett X-tractor sample trays Compatible with 0.2ml tubes Slide 7 7 Gene-Disc rotor ready for cycling Rotor-Gene Gene-Disc Vertical tube orientation means a robot can set up all reactions directly into Gene-Disc tubes The heat sealer provides a permanent or removable film seal (user selectable switch) Slide 8 8 Position and height Before using the robot each block must have definitions for position then height Once you have specified the position to the middle of well 1 on a selected plate all other wells are automatically calibrated Height is defined either using either automatic or manual calibration Recommend checking height automatically first then repeat manually, especially if using small volumes If unsure do not change position and height as this will affect all users Slide 9 9 Plate position Select Options on the toolbar, then Robot Setup, Calibrate plate positions Choose plate then use X and y adjustments to select the middle of the first well Slide 10 10 Plate height Select Options, Robot Setup, Calibrate plate heights Warning comes up to make sure you know what you are doing Select plate, change 4 to 1, click autodetect Repeat, change 4 to 1, and use manual Setup, lower tip until it feels tighter Slide 11 11 Sample names and IDs can be imported from CSV or tab delineated files (also excel) Slide 12 12 Starting the run Click green arrow button on top toolbar Slide 13 13 During the run Slide 14 14 After the run Slide 15 15 Error Messages Slide 16 16 Tip usage options Robot can re-use tips when dispensing mastermix Tip can be re-used 96 times if required We recommend re-use 8-12 times Tip re-use is less precise than single use tips Tips cant be re-used for samples going into a wet well Robot programmed not to allow a tip that has been in a wet well (e.g. with MM) to be re-used If pipetting sample into a dry well the same tip can be used Slide 17 17 Jennifer McMahon 0414 278 369 Phone:02 9736 1320 FAX:02 9736 1364 Freecall:1800 803 915 www.corbettresearch.com Slide 18 18 Rotor-Gene 3000 results CAS-1200 robot C T std dev 0.05 20 L reaction volume, 18 replicates Hand pipetting C T std dev 0.07 Slide 19 19 Rotor-Gene 3000 results 15 L reaction volume, 18 replicates Hand pipetting C T std dev 0.11 CAS-1200 robot C T std dev 0.07 Slide 20 20 Rotor-Gene 3000 results 10 L reaction volume, 18 replicates Hand pipetting C T std dev 0.12 CAS-1200 robot C T std dev 0.10 Slide 21 21 Rotor-Gene 3000 results 5 L reaction volume, 18 replicates Hand pipetting C T std dev 0.64 CAS-1200 robot C T std dev 0.12