09th Apr 2016

The project has been approved on Jan 15, 2013, and was initiated the same day. From that time forward, we have received, all agreed funds (not all) towards purchase of the

project. The Progress report is due for the period of 31-36th month period. According to agreed objectives, we have met the required targets, and we are proceeding with great strength and

intended goal.

Objectives To Get To The Purpose

Period of

Study

(In Months)

Achievable Targets

(Physical and Technical)

Current

Status

0-6

1. Phenotypic identification of the diverse maize germplasm for carotenoid content

2. Donor plants carrying opaque 2 will be crossed with our NSL elite lines to generate F1

progeny

√

7-12

1. Identification of donors with superior levels of carotenoids, to use in the crossing program

and transfer superior carotenoid alleles into elite lines.

2. F1 progeny for QPM will be backcrossed with elite recurrent parent to generate BC1F1

√

13-18

1. Standard PCR based cloning techniques, genome walking, sequencing, and data analysis

using bio-informatics tools

2. BC1F1 will be self-pollinated to produce BC1F2 and the same will undergo for foreground

selection for opaque 2 using gene specific markers

3. Lines carrying opaque 2 will be screened for background selection using recurrent parent √

Stacking Traits….Along with Profit.

13-183. Lines carrying opaque 2 will be screened for background selection using recurrent parent

specific SSR markers.

4. Donor plants carrying superior carotenoid alleles will be crossed with elite lines to

generate F1 progeny

√

19-24

1. F1 progeny carrying superior carotenoid alleles will be backcrossed with elite recurrent

parent to generate BC1F1, while performing biochemical and molecular analysis.

2. BC1F2 carrying opaque2 will be self-pollinated to generate BC1F3 and will undergo for

foreground and background selection

√

25-30

1. BC1F1 carrying superior carotenoid alleles will be self-pollinated, to generate BC1F2, while

performing biochemical and molecular analysis.



√

31-36





In

Process

37-42

1. BC1F5 carrying opaque2 will be self-pollinated to generate BC1F6 (QPM Fixed elite line)

and will undergo for foreground and background selection



In

Process

43-481. Fixed QPM elite line will be crossed with BC1F4, carrying superior carotenoid alleles, to

generate F1 carrying both traits of interest.

49-54 1. F1 progeny will be self-pollinated to generate F2

55-60

1. F2 population seed will be subjected to both morphological, biochemical, and molecular

analysis for both traits of interest.

2. Selected lines will be further self-pollinated and continued to fix the traits.

and was initiated the same day. From that time forward, we have received, all agreed funds (not all) towards purchase of the intended equipment, consumables related to

month period. According to agreed objectives, we have met the required targets, and we are proceeding with great strength and full speed towards achieving the

Objectives To Get To The Purpose

Current

Status

√

√

√

Stacking Traits….Along with Profit.

Uniq

ue S

elli

ng P

oin

t (U

SP

)

and S

ocia

l

sib

ility

√

√

√

In

Process

In

Process

Uniq

ue S

elli

ng P

oin

t (U

SP

)

Com

merc

ial V

entu

reand S

ocia

l

Corp

ora

te r

esponsib

Back Cross scheme, using conventional Genetic component Genetic System Conventional breeding

o2 The recessive mutant allele of the o2 gene Phenotypic observation in F2

Hardness modifiers The endosperm hardness modifier genes/QTL Through light box screening

Amino acid modifiers The amino acid modifier genes/QTL influencing amino acid content in the endosperm

Through estimation of Lysine and Tryptophan

SELFING

PopulationPopulation

SELFING

X

Foreground selection and limited

background selection

Determination of optimum population size

for BC2




for BC2

Progeny

F1

Elite LineElite LineX

BC1F1

Population

Foreground selection

background selection of recurrent parent

Identification of individuals with highest

recurrent genome content





PopulationBC1F2

SELFING

al and MAS to convert normal to QPM lines Conventional breeding Molecular breeding Current Status

Phenotypic observation in F2 Using SSRs with in o2 in BC and F2

Standardized the method is currently in use

Through light box screening Markers??? Standardized the way and is currently in use

Through estimation of Lysine and Tryptophan

Markers??? In progress

SELFING

SELFING

PopulationBC1F3

Population

SELFING

Select agronomicallyand biochemically

superior BC2F3 line

BC1F4 Population

Select agronomicallyand biochemically

superior BC2F3 line

--







Line


Phenotypic selection for improved

agronomic characters

Phenotypic selection for kernel

modification and tryptophan conc.


Phenotypic selection for improved

agronomic characters

Phenotypic selection for kernel

modification and tryptophan conc.









PopulationQPM version

of elite line

Foreground Selection of BC1F2 progenies for

P1 P2 O O O O H H O H H H H H O N

N H N O H N N N

P1 P2 H H H H O O H H H H H O H N

H O H H O H N N

P1 P2 O H H H O H H H H H H O H H

H O H H H H H O

P P H H H N N N N H N N H O H H P1 P2 H H H N N N N H N N H O H H

N N H O N N O N

P1 P2 O N O N N N N H H H H H H H

O H O N N H H O

P1 P2 N H H O H H H H H H H O O N

H H O H O H O H

P1 P2 H H H O O H O H H H O H O H

H H N H O O O

Foreground Selection of BC1F2 progenies for opaque2 allele

O O O O H H O H H H H H O N

H H H H O O H H H H H O H N

H H H N N N N H N N H O H H H H H N N N N H N N H O H H

O N O N N N N H H H H H H H

N H H O H H H H H H H O O N

H H H O O H O H H H O H O H

Elite parents selected for the

work

3001 3003 M183

1 O2o2 [Hetero] + Elite type 46 40 22

2 o2o2only 24 35 11

3 O2o2 [Hetero] only 66 20 18

4 Normal 40 45 50

III III

Hardness of endosperm - Scoring of Endosper

IV V

A212 B114

Type (Modification Score) I: Not opaque

Type (Modification Score) II: 25% opaque

Type (Modification Score) III: 50% opaque

Type (Modification Score) IV: 75% opaque

erm modification through conventional light box

G270

Type (Modification Score) IV: 75% opaque

Type (Modification Score) V: 100% opaque

Estimation of Tryptophan in maize donor parent seed and recurrent parent seed

Donors Elite Cultures

BC1F3 (Bulk)

P

Estimation of Tryptophan in maize donor parent seed and recurrent parent seed

Elite Cultures

BC1F3 (Bulk)

P

A

A

A

B

B

1.01

1.02

1.03

1.04

1.05

1.06

1.0

2.01

2.02

2.03

2.04

2.05

2.0

bnlg1179

bnlg1007

bnlg1203

bnlg1811

umc2083

umc1035

umc1335

bnlg1092

bnlg1017

mmc0111

bnlg2248

mmc0231

bnlg1175

phi049

bnlg1144

bnlg1325

bnlg1447

bnlg1113

B

G

G

B

B1.07

1.09

1.08

1.10

1.11

1.12

2.05

2.06

2.07

2.08

2.09

2.10

bnlg1629

phi037

bnlg1502

umc1802

umc1737

bnlg1055

phi120

umc1605

bnlg1036

bnlg1887

bnlg2077

bnlg2144

bnlg1267

bnlg1746

mmc0381

phi1099

bnlg1904

bnlg2136

bnlg505

bnlg2242

dupssr23

bnlg1605

umc1148

bnlg1182

1

23

3.0

3.01

3.02

3.03

3.04

4.01

4.0

4.02

4.03

4.04

5.01

5.02

5.0

5.03

5.04

7.0

7.01

7.02

9.0

9.02

9.03

9.01

10.0

10.01

10.02

phi049

bnlg1144

bnlg1325

bnlg1447

bnlg1113

umc2148

bnlg1126

bnlg1162

bnlg1741

bnlg1265

mmc0321

bnlg1066

mmc0121

bnlg105

bnlg1879

bnlg1046

bnlg1902

bnlg2323

6.0

6.01

bnlg1043

bnlg1600

bnlg1432

dupssr18

bnlg1642

bnlg2132

bnlg1200

bnlg10708.01

8.0bnlg1194

bnlg2235

bnlg2289

bnlg1272

bnlg1288

bnlg1401

dupssr19

umc1191

bnlg1688

bnlg1451

mmc0501

bnlg1655

umc1863

3.05

3.06

3.07

3.08

3.09

4.05

4.06

4.07

4.08

4.09

4.11

4.10

5.05

5.06

5.07

5.08

5.09

7.03

7.04

7.05

7.06

9.04

9.06

9.07

9.08

9.05

10.03

10.04

10.05

10.06

10.07

bnlg1904

bnlg2136

bnlg505

bnlg2242

dupssr23

bnlg1605

umc1148

bnlg1182

bnlg1137

bnlg2291

dupssr34

bnlg1444

bnlg2244

dupssr28

umc1834

bnlg1237

mmc0282

mmc0481

bnlg1306

bnlg1711

6.02

6.03

6.04

6.05

6.06

6.07

6.08

dupssr18

umc1083

umc2316

bnlg1154

bnlg1617

bnlg2249

bnlg345

bnlg1740

umc2165

umc1001

bnlg1666

umc1799

phi057

phi116

8.01

8.02

8.03

8.04

8.05

8.06

8.07

8.08

8.09

umc1415

bnlg1176

bnlg1146

bnlg1031

bnlg1828

bnlg1056

dupssr14

bnlg1688

bnlg1270

umc2346

bnlg1129

bnlg1588

umc1077

bnlg1185

bnlg2190

bnlg1480

3 4

5

6

7

8

9

10

Total Markers screened:200Best Markers: 77

77 are being used to recover the

recurrent genome

Elite QPM Het. Elite QPM Het.

4 60 27 14 30 72 10 17

24 52 26 22 23 72 15 13

11 54 24 24 17 70 14 16

6 61 23 16 12 67 15.4 17.6

1 56.6 21.1 22.3 27 63 20 17

9 57 18 24 2 62.3 12 25.7

32 54 17 29 26 74 10 16

13 58.1 16.2 25.7 28 73 12 16

25 33.7 28.9 37.4 22 71 13 17

21 57 24 18 31 70 7.9 22

20 56 21 23 5 69 12 19

10 58 21 21 19 69 15 16

27 63 20 17 8 68 14 19

33 67 17 16 33 67 17 16

18 54.3 17.2 28.5 15 63 13 24

3 54.95 17.03 17.02 16 63 14 23

QP

M P

lan

ts i

n L

OT

-A

Plant

No.

%of genome

ELI

TE

Pla

nts

in

LO

T-A

Plant

No.

%of genome

Selection of Best Elite and QPM counterparts for [F1] Hybridization program (pre


2 37.7 23.4 38.9 1 70.0 9.4 20.6

3 54.2 16.7 29.2 5 61.0 15.7 23.3

4 57.7 20.8 21.4 12 58.6 16.0 25.3

7 47.7 20.1 32.2 13 58.4 13.7 28.0

8 42.3 23.3 34.4 9 57.6 18.8 23.6

9 57.6 18.8 23.6 14 43.6 13.3 43.0

12 58.6 16 25.3 17 53.0 16.7 30.4

15 45 25.4 29.6 10 75.4 15.0 9.6

16 50 18.7 31.3 19 74.6 10.1 15.4

17 53 16.7 30.4 18 64.3 18.5 17.3

18 64.3 18.5 17.3 4 57.7 20.8 21.4

20 50.6 16.3 33.1 3 54.2 16.7 29.2

21 53.6 12.7 33.7

Plant

No.

%of genome

QP

M P

lan

ts i

n L

OT

-G

Plant

No.

%of genome

ELI

TE

Pla

nts

in

LO

T-G


17 17 70 14 27 73 11 25

1 14 68 18 4 63 18 27

10 18 67 17 8 60 12 36

23 61 26 17 3 59 20 30

7 53 23 30 18 57 14 29

16 46 21 35 7 53 23 30

3 59 20 30 19 54 15 40

5 52 19 36 24 57 13 26

9 48 18 36 21 46 15 41

6 46 33 26 29 58 12 31

28 50 32 26 23 61 26 17

2 41 30 35 22 61 18 28

15 55 28 26 15 55 28 26

12 44 24 39 11 55 18 35

13 45 24 33 14 55 21 23

14 55 21 33 5 52 19 36

QP

M P

lan

ts i

n L

OT

-B

Plant

No.

%of genome

ELI

TE

Pla

nts

in

LO

T-B

Plant

No.

%of genome

Selection of Best Elite and QPM counterparts for [F1] Hybridization program (pre-commercial activity)


27 42.7 25.2 32.1 1 63.6 20.7 15.7

5 50.7 22.9 26.4 2 61.6 13.7 24.7

10 52.1 20.8 27.1 3 67.4 5.9 26.7

1 63.6 20.7 15.7 6 58.1 10.1 31.8

26 57.0 18.3 24.6 7 68.7 6.8 24.5

14 58.1 18.2 23.6 9 65.5 9.5 25.0

22 55.8 15.9 28.3 11 63.1 14.1 22.8

13 54.5 14.5 31.0 12 58.8 8.8 32.4

19 57.9 14.5 27.6 15 67.2 10.2 22.6

11 63.1 14.1 22.8 16 73.3 10.4 16.3

16 73.3 10.4 16.3 17 62.1 13.1 24.8

23 60.2 26.6 13.3 18 62.2 10.4 27.4

2 61.6 13.7 24.7 20 71.2 9.4 19.4

17 62.1 13.1 24.8 21 78.8 6.8 14.4

28 58.7 13.0 28.3 23 60.2 26.6 13.3

24 54.7 12.8 32.4 25 67.1 7.7 25.2

18 62.2 10.4 27.4 28 58.7 13.0 28.3

15 67.2 10.2 22.6 29 66.7 8.3 25.0

Plant

No.

%of genome

QP

M P

lan

ts i

n L

OT

-F

Plant

No.

%of genome

ELI

TE

Pla

nts

in

LO

T-F

1 3 6 10 14 18 32 5 7 8 11 12 17 22 23 26 31 33

3 X X X X

7

8

18 X X X

20

21 X X X X X X

23 X X

24 II

29

2 X X

5 X X

6 X

LOT A QPM LINES

LOT

B L

ine

s

LOT

F L

ine

s

Cross Hybridization program (pre-commercial activity) keeping quality and quantity in harmony

6 X

9 II X X X X

12 X X X X X II

13 X X

15 X II X X X X

16 X X X X X X X X

22 X

25

26 II X X X X X

28 X X X

Selected for Elite line based on genome recovery data

Selected for QPM line based on genomerecovery data

selected for both Elite & QPM nature based on genome recovery data

LOT

B L

ine

s

LOT

F L

ine

s

1 2 4 5 6 7 8 10 10 11 11 12 13 13 13 14 15 16 16 17 17 18 19 19 21 21

1 X X X X X X X X X

2 X X X X

3 II X X X X X X

4 X X X X X X

5 X X X X X X X X X

6 X X X X II X X X X

7 X X X X X X X X X X

8 X X X X X X X X X

9 X X X X X X X X X X X X

10 X X X X X X II X X X X

11 X X X II X

12 X II II II X II II X X X X

13 X X X

14 X X X X X X X X

15 X X

LOT G QPM LINES

LOT

F L

ine

s

commercial activity) keeping quality and quantity in harmony

16 X X X X

17 X X X

18 X

19 X X X X X X X X X

20 X X X X X X X


22 X X X X X X

23 X X X

24 X X X X X X X X

25 X X X X


27 X X X X X X

28 X X X X

29 X X X X X

Selected for Elite line based on genome recovery data

Selected for QPM line based on genomerecovery data

selected for Elite & QPM both natures based on genome recovery data

LOT

F L

ine

s

TSS

ATG

MGLSGATISAPLGCCVLRCGAVGGGKALKADAERWRRAGWSRRVGGPKVRCVATEKHDETA

AVGAAVGVDFADEEDYRKGGGGELLYVQMQSTKPMESQSKIASKLSPISDENTVLDLVIIGCGP

AGLSLASESAKKGLTVGLIGPDLPFTNNYGVWEDEFKDLGLESCIEHVWKDTIVYLDNNKPILIG

RSYGRVHRDLLHEELLKRCYEAGVTYLNSKVDKIIESPDGHRVVCCDKGREIICRLAIVASGAAS

GRLLEYEVGGPRVCVQTAYGVEVEVENNPYDPSLMVFMDYRDCFKEEFSHTEQENPTFLYAM

PMSPTRVFFEETCLASKDAMSFDLLKKRLMYRLNAMGIRILKVYEEEWSYIPVGGSLPNTDQK

NLAFGAAASMVHPATGYSVVRSLSEAPRYASVISDILGNRVPAEYMLGNSQNYSPSMLAWRTL

WPQERKRQRSFFLFGLALIIQLNNEGIQTFFEAFFRVPRWMWRGFLGSTLSSVDLILFSFYMFAI

APNQLRMNLVRHLLSDPTGSSMIKTYLTL

Zeamays

Complete

Introduction of carotenoid superior alleles controlling the flux towards

AU

0.000

0.010

0.020

Minutes

0.00 2.00 4.00 6.00 8.00 10.00 12.00 14.00 16.00 18.00 20.00 22.00

MAAAMTSFVAKNPLLAAAARRRAPPLAGRALPFSPLASTRAPRRTVTCFVPQDTAAPAAPVPALDEE

ARAAAARRVAEKEARKRSERRTYLVAAVMSSLGVTSMAVAAVYYRFSWQMEGGEVPVIETLGTFAL

SVGAAVGMEFWARWAHRALWHASLWHMHESHHRPREGPFELNDVFAIVNAAPAISLLAYGFFHRG

IVPGLCFGAGLGITLFGMAYMFVHDGLVHRRFPVGPIADVPYFRRVAASHKIHHMDKFGGVPYGLFL

GPKELEEVGGLDELVSSPVSEATDTEDAGEEKTRPVVCVVRTSVFMGQSVPNEF

ATG

Zeamays

Complete

High β-carotene accumulating donor maize line

E1 E3E2 E4 E5 E7E6 E8 E9 E10 E11 E12 E13

354 60 207 126 57 156

181 88 152 197 98TAG

MGLSGATISAPLGCCVLRCGAVGGGKALKADAERWRRAGWSRRVGGPKVRCVATEKHDETA

AVGAAVGVDFADEEDYRKGGGGELLYVQMQSTKPMESQSKIASKLSPISDENTVLDLVIIGCGP

AGLSLASESAKKGLTVGLIGPDLPFTNNYGVWEDEFKDLGLESCIEHVWKDTIVYLDNNKPILIG

RSYGRVHRDLLHEELLKRCYEAGVTYLNSKVDKIIESPDGHRVVCCDKGREIICRLAIVASGAAS

GRLLEYEVGGPRVCVQTAYGVEVEVENNPYDPSLMVFMDYRDCFKEEFSHTEQENPTFLYAM

PMSPTRVFFEETCLASKDAMSFDLLKKRLMYRLNAMGIRILKVYEEEWSYIPVGGSLPNTDQK

NLAFGAAASMVHPATGYSVVRSLSEAPRYASVISDILGNRVPAEYMLGNSQNYSPSMLAWRTL

WPQERKRQRSFFLFGLALIIQLNNEGIQTFFEAFFRVPRWMWRGFLGSTLSSVDLILFSFYMFAI

APNQLRMNLVRHLLSDPTGSSMIKTYLTL

LCYE Gene Structure 5393bp (1778bp)

Zeamays ε–Lycopene cyclase (lcyE) mRNA, lcyEW22 allele

Complete cds GenBank EU924262.1 (1778bp; 537aa)

Introduction of carotenoid superior alleles controlling the flux towards β-carotene

24.00 26.00 28.00 30.00 32.00 34.00 36.00 38.00 40.00 42.00 44.00 5732

MAAAMTSFVAKNPLLAAAARRRAPPLAGRALPFSPLASTRAPRRTVTCFVPQDTAAPAAPVPALDEE

ARAAAARRVAEKEARKRSERRTYLVAAVMSSLGVTSMAVAAVYYRFSWQMEGGEVPVIETLGTFAL

SVGAAVGMEFWARWAHRALWHASLWHMHESHHRPREGPFELNDVFAIVNAAPAISLLAYGFFHRG

IVPGLCFGAGLGITLFGMAYMFVHDGLVHRRFPVGPIADVPYFRRVAASHKIHHMDKFGGVPYGLFL

GPKELEEVGGLDELVSSPVSEATDTEDAGEEKTRPVVCVVRTSVFMGQSVPNEF

TAG

HYD3 gene structure 4990bp (1246)

Zeamays β-carotene hydroxylase (HYD3) mRNA , B73 allele

Complete cds; GenBank: AY844958 1246bp (319aa)

carotene accumulating donor maize line

AU

0.000

0.010

0.020

0.00 2.00 4.00 6.00 8.00 10.00 12.00 14.00 16.00 18.003001

AU

0.010

0.020

0.030

0.040

3003

To Transform NSL Corn Crop Commercial lines with Unique Selling Proposition (USP)

0.000

0.00 2.00 4.00 6.00 8.00 10.00 12.00 14.00 16.00 18.003003

AU

0.00

0.05

0.10

0.15

0.00 2.00 4.00 6.00 8.00 10.00 12.00 14.00 16.00 18.00M183

Lutein Zeaxanthin β-carotene

NSL Corn Crop Commercial lines used in its NMH Hybrid breeding program

Minutes

20.00 22.00 24.00 26.00 28.00 30.00 32.00 34.00 36.00 38.00 40.00 42.00 44.00

To Transform NSL Corn Crop Commercial lines with Unique Selling Proposition (USP)

Minutes

20.00 22.00 24.00 26.00 28.00 30.00 32.00 34.00 36.00 38.00 40.00 42.00 44.00

Minutes

20.00 22.00 24.00 26.00 28.00 30.00 32.00 34.00 36.00 38.00 40.00 42.00 44.00

carotene


D D D D O R R R R R R R R R

Details of ε–LCY gene introgression for Marker


NQMPL's 5730 5732 5723 5721 5734 3001 3003 5896

Polymorphism D D D D O R R R

A 5' indel 2 2 2 2 3 2 - 2

B 3' indel 8 8 8 0 0 0 0 0

C 5' indel 1 2 2 2 1 1 1 1

D 3' indel 1 1 1 1 3 2 4 1

E DEL4 0 12 12 12 0 0 0 0

Class

B: 8= 8bp insertion (140bp); 0=132bp

LCY

HYD

Gene

A: 2= 250bp; 3=250 + 380bp C: 1=800bp; 2=600bp

B

AA

LCY gene introgression for Marker-Assisted Breeding

F2 R2250bp(A)

LCYE 5’ indel (2)

F2 R2

LCYE 5’ indel (3)

F1


250bp + 380bp(A )

8

140bp(B)

LCYE 3’ indel (8)

R2F2

5891 5881 M199 M183 M111 M184

R R R R R R

- - 2 2 2 - 2 STS-Indel Agarose 2.5

0 0 0 8 0 0 8 STS-Indel Agarose 3

1 1 1 1 1 1 2 STS-Indel Agarose 2.5



D: 1= 543bp; 2 = 296bp; 3 = 296 + 1221bp; 4= 296 +875bp

Marker Type PCR-Assay Gel %Favourable

Alleles

E: 0= 117bp; 12=12bp insertion (129bp)

C


D


Details of β–HYD3 gene introgression for Marker


ENon Specific Bands


NQMPL's 5730 5732 5723 5721 5734 3001 3003 5896

Polymorphism D D D D O R R R

A 5' indel 2 2 2 2 3 2 - 2

B 3' indel 8 8 8 0 0 0 0 0

C 5' indel 1 2 2 2 1 1 1 1

D 3' indel 1 1 1 1 3 2 4 1

E DEL4 0 12 12 12 0 0 0 0

Class

B: 8= 8bp insertion (140bp); 0=132bp

LCY

HYD

Gene

A: 2= 250bp; 3=250 + 380bp C: 1=800bp; 2=600bp

C

D

HYD3 gene introgression for Marker-Assisted Breeding

H1 H2

HYD3 5’ indel(C)

(397bp insertion)

800bp(1)

(206bp insertion)

H1 H2

600bp(2)

HYD3 3’ indel (D)

L1 R2

190bp 350bp1

L1 R2

325bp4

1250bp

E

5891 5881 M199 M183 M111 M184

R R R R R R

- - 2 2 2 - 2 STS-Indel Agarose 2.5





D: 1= 543bp; 2 = 296bp; 3 = 296 + 1221bp; 4= 296 +875bp

Marker Type PCR-Assay Gel %Favourable

Alleles

E: 0= 117bp; 12=12bp insertion (129bp)

L1 R2

L1 R2R1

1250bp3

D4F D4R

12bp insertion

I

D4F D4R

D

HYD3 DEL 4 (E)

Is it possible to increase more

Introgress β-carotene accumulating lines into High AU

0.00

0.02

0.04

0.06

0.08

0.10

0.12

0.14

0.16

0.00 2.00 4.00 6.00 8.00 10.00 12.00 14.00 16.00 18.00

0.08

0.10

3876

3113

AU

0.00

0.02

0.04

0.06

0.08

0.00 2.00 4.00 6.00 8.00 10.00 12.00 14.00 16.00 18.00

AU

0.000

0.010

0.020

0.00 2.00 4.00 6.00 8.00 10.00 12.00 14.00 16.00 18.00

3113

5732

Line Development - NSL Corn Crop Commercial lines used in its NMH Hybrid breeding program

Is it possible to increase more β-carotene?

carotene accumulating lines into High Zeaxanthin maize lines

Minutes

20.00 22.00 24.00 26.00 28.00 30.00 32.00 34.00 36.00 38.00 40.00 42.00 44.00

Minutes

20.00 22.00 24.00 26.00 28.00 30.00 32.00 34.00 36.00 38.00 40.00 42.00 44.00

Minutes

20.00 22.00 24.00 26.00 28.00 30.00 32.00 34.00 36.00 38.00 40.00 42.00 44.00


Hyd5'TE Hyd3'TE Del4 Lcye3'TE

HOMO 47 34.0 0.0 34.0 31.9

HETERO 47 17.0 0.0 17.0 12.8

HOMO 44 0.0 22.7 0.0 31.8

HETERO 44 0.0 13.6 0.0 4.5

HOMO 41 29.3 14.6 26.8 31.7

HETERO 41 19.5 29.3 22.0 17.1

HOMO 46 0.0 0.0 0.0 41.3

HETERO 46 0.0 0.0 0.0 0.0

HOMO 40 0.0 2.5 0.0 32.5

HETERO 40 0.0 40.0 0.0 27.5

HOMO 40 0.0 37.5 0.0 32.5

HETERO 40 0.0 20.0 0.0 27.5

HOMO 49 20.4 29.4 12.2 24.5

HETERO 49 34.7 28.6 42.9 28.6

HOMO 38 28.9 23.7 31.6 23.7

HETERO 38 7.9 10.5 10.5 13.2

HOMO 45 31.1 0.0 31.1 22.2

1107X5732

1077X5730

1065X5723

1112X5716

1079X5730

1082X5730

S.NoRabi 2016

field code

1

2

3

4

5

6

7

8

Total%

nature

R1516-139

TO 141

R1516-172

TO 174

R1516-181

R1516-182

TO 185

cross

R1516-134

R1516-186

1120X5732

1064X5723

R1516-215

TO 218

R1516-203

R1516-135

Elite Line development with carotenoid superior

HOMO 45 31.1 0.0 31.1 22.2

HETERO 45 8.9 0.0 8.9 22.2

HOMO 43 0.0 7.0 0.0 20.9

HETERO 43 0.0 32.6 0.0 25.6

HOMO 42 0.0 23.8 0.0 16.7

HETERO 42 0.0 9.5 0.0 16.7

HOMO 48 16.7 4.2 25.0 22.9

HETERO 48 20.8 29.2 4.2 12.5

HOMO 40 32.5 25.0 25.0 25.0

HETERO 40 10.0 12.5 20.0 12.5

HOMO 50 18.0 22.2 16.0 24.0

HETERO 50 10.0 40.0 12.0 10.0

HOMO 42 0.0 14.3 0.0 23.8

HETERO 42 0.0 4.8 0.0 9.5

HOMO 24 0.0 20.8 0.0 29.2

HETERO 24 0.0 12.5 0.0 8.3

HOMO 49 0.0 0.0 22.4 24.5

HETERO 49 0.0 0.0 12.2 8.2

HOMO 49 16.3 16.3 16.3 28.6

HETERO 49 20.4 20.4 20.4 8.2

HOMO 47 0.0 6.4 0.0 21.3

HETERO 47 0.0 17.0 0.0 6.4

HOMO 50 0.0 14.0 0.0 26.0

HETERO 50 0.0 6.0 0.0 6.0

1075x5730

1111X5732

1094X5723

1090X5730

1076X5730

1091X5730

1080X5730

1071X5732

1066X5723

1162X5723

1081X5730

16

17

18

19

20

10

11

12

13

14

15

9

R1516-136

TO 137

R1516-152

TO 154

R1516-155

TO 156

R1516-170

TO 171

R1516-175

TO 178

R1516-138

R1516-187

TO 190

1106X5723

R1516-211

R1516-203

TO 205

R1516-192

TO 196

R1516-131

R1516-132

TO 133


HOMO 40 0.0 17.5 0.0 20.0

HETERO 40 0.0 12.5 0.0 5.0

HOMO 45 0.0 13.3 0.0 0.0

HETERO 45 0.0 24.4 0.0 0.0

HOMO 45 0.0 22.2 0.0 0.0

HETERO 45 0.0 0.0 0.0 0.0

HOMO 50 0.0 18.0 0.0 0.0

HETERO 50 0.0 14.0 0.0 0.0

HOMO 44 0.0 18.2 0.0 0.0

HETERO 44 0.0 0.0 0.0 0.0

HOMO 50 0.0 0.0 0.0 26.0

HETERO 50 0.0 0.0 0.0 0.0

HOMO 45 0.0 13.3 26.7 0.0

HETERO 45 0.0 46.7 37.8 0.0

HOMO 50 0.0 16.0 0.0 0.0

HETERO 50 0.0 44.0 0.0 0.0

HOMHO 48 0.0 18.8 16.7 0.0

%S.No

Rabi 2016

field cross nature Total

1161X5730

1084X5730

1086X5730

1087X619

1088X5730

1092X5730

1069X5732

1089X5730

28

22

23

24

25

26

27

21

R1516-

142

R1516-

143 TO

R1516-

145 TO

R1516-

148

R1516-

R1516-

157 TO

R1516-

163 TO

R1516-

165 TO

R1516-

149 TO

rior alleles for back crossing into QPM fixed lines

HOMHO 48 0.0 18.8 16.7 0.0

HETERO 48 0.0 27.1 25.0 0.0

HOMO 49 0.0 0.0 0.0 18.4

HETERO 49 0.0 0.0 0.0 0.0

HOMO 46 21.7 19.6 21.7 0.0

HETERO 46 2.2 2.2 2.2 0.0

HOMO 47 0.0 0.0 0.0 27.7

HETERO 47 0.0 0.0 0.0 0.0

HOMO 40 32.5 21.5 22.5 0.0

HETERO 40 35.0 42.9 47.5 0.0

HOMO 48 35.4 31.3 33.3 0.0

HETERO 48 0.0 2.1 2.1 0.0

HOMO 50 26.0 26.0 26.0 0.0

HETERO 50 0.0 0.0 0.0 0.0

HOMO 46 32.6 21.7 26.1 0.0

HETERO 46 0.0 8.7 6.5 0.0

HOMO 37 13.5 13.5 13.5 0.0

HETERO 37 0.0 0.0 0.0 0.0

HOMO 44 0.0 0.0 31.8 0.0

HETERO 44 0.0 0.0 0.0 0.0

HOMO 53 32.1 9.4 30.2 0.0

HETERO 53 1.9 7.5 3.8 0.0

HOMO 43 30.2 0.0 25.6 0.0

HETERO 43 2.3 0.0 2.3 0.01113X5721

1164X5723

1100X5721

1101X5721

1102X5721

1103X5721

1109X5721

1098X5723

1095X5723

1097X5723

1121X5723

1123X5723

40

34

35

36

37

38

39

29

30

31

32

33

R1516-

197 TO

R1516-

199

R1516-

200 TO

R1516-

222 TO

R1516-

224 TO

R1516-

227 TO

R1516-

206 TO

R1516-

208 TO

R1516-

212 TO

R1516-

230 TO

R1516-

234 TO

R1516-

219 TO

(Part -1)


homo 48 37.5 29.2 27.1 29.2

hetero 48 8.3 12.5 14.6 14.6

homo 48 0.0 22.9 0.0 29.2

hetero 48 0.0 10.4 0.0 12.5

homo 46 0.0 21.7 0.0 21.7

hetero 46 0.0 4.3 0.0 6.5

homo 48 0.0 0.0 0.0 29.2

hetero 48 0.0 0.0 0.0 0.0

homo 42 0.0 0.0 0.0 19.0

hetero 42 0.0 0.0 0.0 0.0

homo 48 0.0 0.0 0.0 18.8

hetero 48 0.0 0.0 0.0 0.0

homo 43 0.0 0.0 0.0 27.9

hetero 43 0.0 0.0 0.0 0.0

homo 38 34.2 34.2 34.2 26.3

hetero 38 7.9 7.9 7.9 15.8

homo 49 0.0 0.0 0.0 26.5

S.NoRabi 2016

field codecross nature Total

%

49R1516-300

M184X5730

47 R1516-299 5884X5730

48R1516-344

TO 34810602X5723

45R1516-267

TO 2685881X5730

46 R1516-266 5891X5730

43R1516-251

TO 2521017x5730

44R1516-248

TO 2505883X5716

41R1516-242

TO 2445883x5732

42R1516-240

TO 2415883x5730

Elite Line development with carotenoid superior

homo 49 0.0 0.0 0.0 26.5

hetero 49 0.0 0.0 0.0 0.0

homo 44 0.0 0.0 0.0 25.0

hetero 44 0.0 0.0 0.0 0.0

homo 21 9.5 0.0 9.5 14.3

hetero 21 9.5 0.0 4.8 9.5

homo 22 45.5 31.8 40.9 27.3

hetero 22 9.1 13.6 13.6 31.8

homo 19 31.6 31.6 31.6 31.6

hetero 19 5.3 5.3 5.3 15.8

homo 38 21.1 13.2 13.2 28.9

hetero 38 13.2 18.4 18.4 5.3

homo 47 0.0 0.0 12.8 0.0

hetero 47 0.0 0.0 0.0 0.0

homo 49 0.0 0.0 28.6 22.4

hetero 49 0.0 0.0 8.2 6.1

homo 47 0.0 0.0 23.4 0.0

hetero 47 0.0 0.0 0.0 0.0

homo 50 0.0 0.0 12.0 22.0

hetero 50 0.0 0.0 10.0 6.0

homo 49 0.0 0.0 26.5 30.6

hetero 49 0.0 0.0 10.2 10.2

homo 37 0.0 0.0 0.0 27.0

hetero 37 0.0 0.0 0.0 0.0

59R1516-269

TO 2735881X5732

60R1516-351

TO 35210590X5716

57R1516-311

TO 3135894X5732

58R1516-314

TO 31610589X5732

55 R1516-310 M114X5732

56R1516-285

TO 287M111X5732

53R1516-289

TO 291M111X5723

54 R1516-343 10603X5723

51R1516-274

TO 2755881X5723

52R1516-253

TO 2561017X5723

49R1516-300

TO 301M184X5730

50R1516-295

TO 298M114X5730


homo 45 0.0 0.0 0.0 22.2

hetero 45 0.0 0.0 0.0 0.0

homo 36 0.0 0.0 0.0 16.7

hetero 36 0.0 0.0 0.0 0.0

homo 31 0.0 0.0 0.0 16.1

hetero 31 0.0 0.0 0.0 0.0

homo 49 0.0 12.2 16.3 0.0

hetero 49 0.0 0.0 0.0 0.0

homo 40 0.0 0.0 0.0 17.5

hetero 40 0.0 0.0 0.0 0.0

homo 46 0.0 23.9 23.9 21.7

hetero 46 0.0 10.9 10.9 8.7

homo 45 17.8 22.2 20.0 0.0

hetero 45 0.0 0.0 2.2 0.0

homo 45 15.6 17.8 26.7 33.3

hetero 45 24.4 13.3 17.8 13.3

homo 43 0.0 14.0 20.9 20.9

S.NoRabi 2016

field cross

69R1516-

10590X5732

67R1516-

329 TO 10594X5732

68R1516-

325 TO 10603X5732

65R1516-

353 TO 10603X5716

66R1516-

337 TO 10608X5732

63R1516-

292 TO M111X5716

64R1516-

31710604X5732

61R1516-

2765881X5716

62R1516-

263 TO 5896X5716

nature Total%

rior alleles for back crossing into QPM fixed lines

homo 43 0.0 14.0 20.9 20.9

hetero 43 0.0 11.6 11.6 14.0

homo 43 0.0 11.6 0.0 34.9

hetero 43 0.0 2.3 0.0 7.0

homo 50 0.0 8.0 10.0 0.0

hetero 50 0.0 0.0 2.0 0.0

homo 43 0.0 20.9 0.0 27.9

hetero 43 0.0 16.3 0.0 11.6

homo 43 0.0 16.3 0.0 0.0

hetero 43 0.0 0.0 0.0 0.0

homo 41 0.0 12.2 17.1 0.0

hetero 41 0.0 0.0 0.0 0.0

homo 40 15.0 15.0 17.5 20.0

hetero 40 5.0 5.0 2.5 7.5

homo 48 18.8 14.6 18.8 31.3

hetero 48 10.4 0.0 10.4 8.3

homo 47 0.0 0.0 0.0 29.8

hetro 47 0.0 0.0 0.0 0.0

homo 46 0.0 0.0 0.0 19.6

hetero 46 0.0 0.0 0.0 0.0

homo 48 0.0 16.7 22.9 8.3

hetero 48 0.0 4.2 4.2 16.7

homo 48 0.0 16.7 0.0 0.0

hetero 48 0.0 0.0 0.0 0.0

79R1516-

3496192X5723

80R1516-

3506188 X 5723

77R1516-

357 TO 10601X5716

78R1516-

355 TO 10591X5716

75R1516-

321 TO 10591X5732

76R1516-

319 TO 10599X5732

73R1516-

3025894X5730

74R1516-

34210594X5723

71R1516-

33610597X5732

72R1516-

303 TO 10600X5730

69R1516-

31810590X5732

70R1516-

279 TO 5892X5730

(Part -2)

“Food security” is “

Thank You!

“National Security”

09th Apr 2016

Documents

Transcript of 09th Apr 2016