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01 - Phosphate Buffer
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0.2 M Ph hosp pate B Buffer (Stock k Solu utions s) to make 0.1 M Ph hosphate e buffer, pH 7 7.4 Use 11 parts of f Stock A A, 39 parts o of Stock B in a t total of 100 p parts CAUTION - CHEC CK COR RRECT CHE EMICAL FORM MULA AND M MOLECULAR R WEIGHT STOCK A 0.2 M N NaH2PO4 dd H2O 100 mL 250 mL 500 mL 1 L NaH2PO4 F.W. = 120.00 use x grams: 2.40 6.00 12.00 24.00 STOCK B 0.2 M N Na2HPO4 dd H2O 100 mL 250 mL 500 mL 1 L Na2HPO4 F.W. = 141.96 use x grams: 2.84 7.10 14.20 28.39 OR USE: STOCK A 0.2 M N NaH2PO4 dd H2O 100 mL 250 mL 500 mL 1 L NaH2PO4-H2O F.W. = 137.99 use x grams: 2.76 6.90 13.80 27.60 OR USE: STOCK B 0.2 M N Na2HPO4 dd H2O 100 mL 250 mL 500 mL 1 L Na2HPO4-7H2O F.W. = 267.96 use x grams: 5.36 13.40 26.80 53.59 EM-CORE PROTOCOL 01 Timo Protocol # 01 - Filename: 01 - Phosphate Buffer.numbers - updated November 1, 2007 TM - page 1 of 1
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Buffer
Transcript of 01 - Phosphate Buffer
0.2 M Phospate Buffer (Stock Solutions)0.2 M Phospate Buffer (Stock Solutions)0.2 M Phospate Buffer (Stock Solutions)0.2 M Phospate Buffer (Stock Solutions)0.2 M Phospate Buffer (Stock Solutions)0.2 M Phospate Buffer (Stock Solutions)0.2 M Phospate Buffer (Stock Solutions)0.2 M Phospate Buffer (Stock Solutions)to make 0.1 M Phosphate buffer, pH 7.4to make 0.1 M Phosphate buffer, pH 7.4to make 0.1 M Phosphate buffer, pH 7.4to make 0.1 M Phosphate buffer, pH 7.4
Use 11 parts of Stock A, 39 parts of Stock B in a total of 100 parts Use 11 parts of Stock A, 39 parts of Stock B in a total of 100 parts Use 11 parts of Stock A, 39 parts of Stock B in a total of 100 parts Use 11 parts of Stock A, 39 parts of Stock B in a total of 100 parts Use 11 parts of Stock A, 39 parts of Stock B in a total of 100 parts Use 11 parts of Stock A, 39 parts of Stock B in a total of 100 parts
CAUTION - CHECK CORRECT CHEMICAL FORMULA AND MOLECULAR WEIGHTCAUTION - CHECK CORRECT CHEMICAL FORMULA AND MOLECULAR WEIGHTCAUTION - CHECK CORRECT CHEMICAL FORMULA AND MOLECULAR WEIGHTCAUTION - CHECK CORRECT CHEMICAL FORMULA AND MOLECULAR WEIGHTCAUTION - CHECK CORRECT CHEMICAL FORMULA AND MOLECULAR WEIGHTCAUTION - CHECK CORRECT CHEMICAL FORMULA AND MOLECULAR WEIGHTCAUTION - CHECK CORRECT CHEMICAL FORMULA AND MOLECULAR WEIGHT
STOCK A 0.2 M NaH2PO40.2 M NaH2PO4
dd H2O 100 mL 250 mL 500 mL 1 L
NaH2PO4 F.W. = 120.00 use x grams: 2.40 6.00 12.00 24.00
STOCK B 0.2 M Na2HPO40.2 M Na2HPO4
dd H2O 100 mL 250 mL 500 mL 1 L
Na2HPO4 F.W. = 141.96 use x grams: 2.84 7.10 14.20 28.39
OR USE:
STOCK A 0.2 M NaH2PO40.2 M NaH2PO4dd H2O 100 mL 250 mL 500 mL 1 L
NaH2PO4-H2O F.W. = 137.99 use x grams: 2.76 6.90 13.80 27.60
OR USE:
STOCK B 0.2 M Na2HPO40.2 M Na2HPO4dd H2O 100 mL 250 mL 500 mL 1 L
Na2HPO4-7H2O F.W. = 267.96 use x grams: 5.36 13.40 26.80 53.59
EM-CORE PROTOCOL 01
Timo Protocol # 01 - Filename: 01 - Phosphate Buffer.numbers - updated November 1, 2007 TM - page 1 of 1
