WHATISFUNCTIONALGENOMICS?ALISTAIRMARTINCRUKBIOINFORMATICSSUMMERSCHOOLJULY23RD2018

Func%onalGenomicsislikeTeenageSex•  Everyonetalksaboutit.•  Nobodyreallyknowshowtodoit.•  Everyonethinkseveryoneelseisdoingit.•  Soeveryoneclaimstheyaredoingit.

StolenfromDanAriely(talkaboutbigdata)2

WHATISFUNCTIONALGENOMICS?

WHATISFUNCTIONALGENOMICS?

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Determina%onoftherela%onshipbetweenanorganism’sgenomeanditsphenotype

WHATISFUNCTIONALGENOMICS?

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Determina%onoftherela%onshipbetweenanorganism’sgenomeanditsphenotype

WHATISFUNCTIONALGENOMICS?

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Determina%onoftherela%onshipbetweenanorganism’sgenomeanditsphenotype

Slightvaria%oningenome

(~0.1%different)

WHATISFUNCTIONALGENOMICS?

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Determina%onoftherela%onshipbetweenanorganism’sgenomeanditsphenotype

Novaria%oningenome

WHATIS(NOT)FUNCTIONALGENOMICS?

Thursday,19July18View<HeadersandFooters>toalterthistext

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WHATISFUNCTIONALGENOMICS?

TypicalfuncHonalgenomicsquesHons:•  Whenandwherearegenesexpressed?•  Howdogeneexpressionlevelsdifferinvariouscelltypesandstates?•  WhatarethefuncHonalrolesofdifferentgenesandinwhatcellularprocessesdo

theyparHcipate?•  Howaregenesregulated?•  WherearetheacHvegenepromotersinaparHcularcelltype?•  Howdogenesandgeneproductsinteract?•  Howdoesgeneexpressionchangeinvariousdiseasesorfollowingatreatment?

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WHATISFUNCTIONALGENOMICS?

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•  Variantdetec%on•  aCGH•  FISH•  Sanger

•  Epigene%cs•  ChIP-seq•  Bisulfiteseq

WHATISFUNCTIONALGENOMICS?

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•  Transcriptomics•  NorthernBlot•  qPCR•  RNA-seq

WHATISFUNCTIONALGENOMICS?

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•  Transcriptomics•  Ribo-seq

WHATISFUNCTIONALGENOMICS?

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•  Proteomics•  MS•  CyTOF•  Crystallography

WHATISFUNCTIONALGENOMICS?

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Single-cellsequencing

scRNAseqonXenogradtumour

WHATISFUNCTIONALGENOMICS?

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ACKNOWLEDGEMENTS

ContribuHonstoslides:BeniltonSCarvalho

NunoBarbosa-MoraisStephenJEglanNatalieThorne

RoryStark