WHAT IS FUNCTIONAL GENOMICS? - GitHub Pages · WHAT IS FUNCTIONAL GENOMICS? Typical funconal...
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WHAT IS FUNCTIONAL GENOMICS? ALISTAIR MARTIN CRUK BIOINFORMATICS SUMMER SCHOOL JULY 23 RD 2018
Transcript of WHAT IS FUNCTIONAL GENOMICS? - GitHub Pages · WHAT IS FUNCTIONAL GENOMICS? Typical funconal...
WHATISFUNCTIONALGENOMICS?ALISTAIRMARTINCRUKBIOINFORMATICSSUMMERSCHOOLJULY23RD2018
Func%onalGenomicsislikeTeenageSex• Everyonetalksaboutit.• Nobodyreallyknowshowtodoit.• Everyonethinkseveryoneelseisdoingit.• Soeveryoneclaimstheyaredoingit.
StolenfromDanAriely(talkaboutbigdata)2
WHATISFUNCTIONALGENOMICS?
WHATISFUNCTIONALGENOMICS?
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Determina%onoftherela%onshipbetweenanorganism’sgenomeanditsphenotype
WHATISFUNCTIONALGENOMICS?
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Determina%onoftherela%onshipbetweenanorganism’sgenomeanditsphenotype
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Determina%onoftherela%onshipbetweenanorganism’sgenomeanditsphenotype
Slightvaria%oningenome
(~0.1%different)
WHATISFUNCTIONALGENOMICS?
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Determina%onoftherela%onshipbetweenanorganism’sgenomeanditsphenotype
Novaria%oningenome
WHATIS(NOT)FUNCTIONALGENOMICS?
Thursday,19July18View<HeadersandFooters>toalterthistext
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WHATISFUNCTIONALGENOMICS?
TypicalfuncHonalgenomicsquesHons:• Whenandwherearegenesexpressed?• Howdogeneexpressionlevelsdifferinvariouscelltypesandstates?• WhatarethefuncHonalrolesofdifferentgenesandinwhatcellularprocessesdo
theyparHcipate?• Howaregenesregulated?• WherearetheacHvegenepromotersinaparHcularcelltype?• Howdogenesandgeneproductsinteract?• Howdoesgeneexpressionchangeinvariousdiseasesorfollowingatreatment?
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WHATISFUNCTIONALGENOMICS?
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• Variantdetec%on• aCGH• FISH• Sanger
• Epigene%cs• ChIP-seq• Bisulfiteseq
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• Transcriptomics• NorthernBlot• qPCR• RNA-seq
WHATISFUNCTIONALGENOMICS?
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• Transcriptomics• Ribo-seq
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• Proteomics• MS• CyTOF• Crystallography
WHATISFUNCTIONALGENOMICS?
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Single-cellsequencing
scRNAseqonXenogradtumour
WHATISFUNCTIONALGENOMICS?
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ACKNOWLEDGEMENTS
ContribuHonstoslides:BeniltonSCarvalho
NunoBarbosa-MoraisStephenJEglanNatalieThorne
RoryStark
