USE OF PLATELET RICH PLASMA

IN AESTHETIC SURGERY

DR.R.KARTHIK,

Cosmetic Surgeon,

Chennai Plast ic Surgery

• Platelets are living but terminal cytoplasmic portions of

marrow megakaryocytes

• They have no nucleus but have alpha granules which

release growth factors

INTRODUCTION

CoagulationTissue

Healing

PLATELET RICH PLASMA BIOLOGY

PLATELET DERIVED GROWTH FACTORS

Initiates connective tissue healing.

Increases mitogenesis, angiogenesis and macrophage activation

Differentiation of fibroblasts

VASCULAR ENDOTHELIAL GROWTH FACTOR

Possess potent angiogenic properties

Enhances vascular permeability

EPIDERMAL GROWTH FACTOR

Induces epithelial development

Promotes angiogenesis

TRANSFORMING GROWTH FACTOR BETA

Increases chemotaxis and mitogenesis.

Stimulates deposition of collagen

INSULIN LIKE GROWTH FACTOR

EPIDERMAL GROWTH FACTORS

Induces epithelial development

Promotes angiogenesis

PLATELET RICH PLASMA

Normal count : 2 X 105 / µL PRP : 2 million / µL

To be considered PRP, must be 4X or greater above

baseline levels

Increasing the concentration of bioactive proteins acts as

a catalyst for accelerating the wound healing process

• Early technique of isolation was

difficult because of equipment size,

cost, need for perfusionist & large

volume of blood

• The new technique is easy with the

help of AUTOMATED DUAL SPIN

PROCESS MACHINE

HARVEST SmartPREP®2

ISOLATION OF PRP

ISOLATION OF PRP

HARVEST SmartPReP2 SYSTEM

Automated Multibiologic Platform System

(15 mins. processing)

Unsurpassed AutologousBiologic Concentrate

20 – 60 cc of blood withdrawn & placed in atube containing citrate phosphate dextrose asan anticoagulant

APPLICATIONS OF PRP

SKIN REJUVENATION

Fine wrinkles around the eyes

Nasolabial grooves

Wrinkles on the lips

Acne marks

Wrinkles on the forehead & neck

Bags and dark circles under the eyes

Loose, saggy skin

HAIR RESTORATION

FAT FILLS

PRP IN FAT FILLS

• Stimulates mesenchymal stem cells found in fat deposits

(PDGF)

• Increases the adhesion of cells (PDGF)

• Increases Revascularization (PDAF)

• Stimulates Active fibroblast & collagen matrix formation

(PFGF)

• Act as stable scaffolding

ACTION OF PRP

• Increases the graft uptake

• Increases survival rate & longevity of graft

• Decreases the liponecrosis

• Decreases Lipid cyst formation

• Decreases micro calcification especially in large volume

augmentation (breast & buttocks)

ADVANTAGES

Pre - op 1 week post - op