Transcript of Sigma xi test two
- 1. CCR5 32 AS APOTENTIAL GENE THERAPY FOR AIDS CONNOR ANDERSON,
ALEXANDRA FULTON, MEGAN SHELDON, VICTORIASOEWARNA & AMANDA
WORCESTER
- 2. MAKE SURE YOUR VOLUME ISTURNED UP.THERE ARE
VOICEOVERSTHROUGHOUT THEPRESENTATION.PLEASE AND THANK YOU!
- 3. HIV ENTRY
- 4. DELTA 32 Current deletion mutation that results in a frame
shift 32 base pairs are absent from the gene sequence THE CAT AND
DOG RAN FOR FUN A THE CTA NDD OGR ANF ORF UN
- 5. CCR5 GENE SEQUENCE
- 6. TIMOTHY RAY BROWN Had HIV and Leukemia Leukemia was
eradicated from the body HIV status was reversed Genetically
altered genes into a vector Vector to cell line Gene therapy and
treatment
- 7. THE BIG PICTURE Develop an easier gene therapy technique in
hopes ofcuring HIV
- 8. SAMPLE COLLECTION Informed consent form Blood sample
collected fromAF2 Purification of blood sample tocollect
lymphocytes (whiteblood cells) DNA purification
- 9. CCR5 SEQUENCE
- 10. DIGESTIONS Cut open vector to prepare forligation
- 11. PLNCX2 MAP Designed for retroviral gene delivery and
expression
- 12. LIGATION Ligated oligonucleotides onto AF2 Converted BamHI
into HINDIII site, and Xba into NOTI site. Made AF2 compatible for
ligation into pLNCX2sMultiple Cloning Site (MCS).
- 13. BACTERIAL TRANSFORMATION
- 14. TRANSDUCTION RNA is packaged intoretroviral particles
pLNCX2 does notcontain the structuralgenes needed forparticle
formationreplication Genes are stablyintegrated into PT67
Retroviral particles caninfect target cells
- 15. CULTURE CELLS U937 cells possess the CCR5 gene but do
notexpress receptor
- 16. FUTURE PLANS Test for presence of CCR5 in U937 cells