Sigma xi test two
16
CCR5 Δ32 AS A POTENTIAL GENE THERAPY FOR AIDS CONNOR ANDERSON, ALEXANDRA FULTON, MEGAN SHELDON, VICTORIA SOEWARNA & AMANDA WORCESTER
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Transcript of Sigma xi test two
- 1. CCR5 32 AS APOTENTIAL GENE THERAPY FOR AIDS CONNOR ANDERSON, ALEXANDRA FULTON, MEGAN SHELDON, VICTORIASOEWARNA & AMANDA WORCESTER
- 2. MAKE SURE YOUR VOLUME ISTURNED UP.THERE ARE VOICEOVERSTHROUGHOUT THEPRESENTATION.PLEASE AND THANK YOU!
- 3. HIV ENTRY
- 4. DELTA 32 Current deletion mutation that results in a frame shift 32 base pairs are absent from the gene sequence THE CAT AND DOG RAN FOR FUN A THE CTA NDD OGR ANF ORF UN
- 5. CCR5 GENE SEQUENCE
- 6. TIMOTHY RAY BROWN Had HIV and Leukemia Leukemia was eradicated from the body HIV status was reversed Genetically altered genes into a vector Vector to cell line Gene therapy and treatment
- 7. THE BIG PICTURE Develop an easier gene therapy technique in hopes ofcuring HIV
- 8. SAMPLE COLLECTION Informed consent form Blood sample collected fromAF2 Purification of blood sample tocollect lymphocytes (whiteblood cells) DNA purification
- 9. CCR5 SEQUENCE
- 10. DIGESTIONS Cut open vector to prepare forligation
- 11. PLNCX2 MAP Designed for retroviral gene delivery and expression
- 12. LIGATION Ligated oligonucleotides onto AF2 Converted BamHI into HINDIII site, and Xba into NOTI site. Made AF2 compatible for ligation into pLNCX2sMultiple Cloning Site (MCS).
- 13. BACTERIAL TRANSFORMATION
- 14. TRANSDUCTION RNA is packaged intoretroviral particles pLNCX2 does notcontain the structuralgenes needed forparticle formationreplication Genes are stablyintegrated into PT67 Retroviral particles caninfect target cells
- 15. CULTURE CELLS U937 cells possess the CCR5 gene but do notexpress receptor
- 16. FUTURE PLANS Test for presence of CCR5 in U937 cells