Renal Ultrastructure Atlas

Wm. J. Hunter M. D.

How to Use This Atlas

• These photos are meant to supplement your Jungueire text-atlas and Robbins by giving some different views.

• Generally I put an unlabeled photo on one page and the labeled view on the next. Try to identify the cells and structures first before looking at the labels.

• This atlas is posted in blackboard-- the resolution on screen is much better than in this handout.

• Please contact me if you have any questions.

Normal gomerulus

Epithelial cell(podocyte) parietal cell prox. tubule

Endothelial cell mesangial cell capillary lumen

Diagrammaticview of low power EM

Low power view ofNormal glomerulus

Low power view ofNormal glomerulus

Endothelial cell nucleus

Capillary lumen

Mesangial cell

Endothelial cell

Epithelial cell (podocyte)

Bowman’s space (urinarySpace)

Capillary lumen

RBC

Medium power EM ofa capillary loop

Medium power EM ofa capillary loop

Capillarylumen

Foot processes

Mesangial cell cytoplasm

Mesangial matrix

Parietal layer of bowman’scapsule

Urinary space

Endothelial cell nucleus

Endothelial cell fenestrations

Epithelial cell (podocyte)

Another capillary loop

Another capillary loop

Epithelial cell (podacyte)

Capillary lumen

RBC

Mesangial cell

Urinary space

The glomerular capillarybasement membrane

The glomerular capillarybasement membrane

Capillary lumen

Urinary space

Foot process

Epithelial cell

Endothelial cell

BM

Close up

of BM

Close up of Basement Membrane

Capillary lumen

Endothelial cell

fenestration

Lamina densa

Lamina raraexterna

Lamina rarainterna

Urinary space

Foot process

BM

Slit membrane

Epithelial cell

Proximal tubules- note hard to see cell borders

microvilli

Low power of proximaltubule

Low power of proximal tubule

Brush border

Tubular BM

InterdigitatedLateral process

Distal tubule

Distal tubule

lumenPlasma cell

Interstitial cell

Short villi

Collecting tubule/duct

Collecting tubule/duct

lumen

Classic Glomerular Disorders

• Please refer to these cases as we discuss them in class

• Think about the classic picture on light microscopy, immunofluorescence and electron microscopy and

• Explain the pathophysiology of the diseases

Acute proliferative

GN (post strep)

Acute proliferative

GN (post strep)

Hypercellular glomeruliIncreased # of cells

Normal glomerulus

IF showing “lumpy-bumpy” pattern

Minimal changedisease

Minimal changedisease

Minimal change disease “normal” glomerulus by light microscopy

Minimal changedisease

Minimal changedisease

Membranous nephropathy thickened BM

Membranous GN

Membranous GN

Membranous GN

Membranous GN

Membranous GN

Diffuse proliferative lupus GN (WHO class IV) all glomeruli involved

Endothelial, epithelial, & mesangial proliferation, thrombosis of Capillaries (note few capillary lumens seen)

IF in lupus nephritis anti-IgG

Proliferative lupus nephritis wire loop--subendothelial deposit

Lupus nephritis-Wire loops

Lupus nephritis-Wire loops

Lupus nephritis-Wire loops

Lupus nephritis-Wire loops

Lupus nephritis-Wire loops

Lupus nephritis-Wire loops

Lupus nephritis-Wire loops