Post on 31-Mar-2020
Microwave Mediated Green Synthesis Of Copper Nanoparticles
Using Aqueous Extract Of Piper Nigrum Seeds And Particles
Characterisation
N. Gandhi1 D. Sirisha2 * and Smita Asthana3 1. Research Scholar, Centre for Environment and Climate Change, School of Environmental Sciences,
Jawaharlal Nehru Institute of Advanced Studies, Hyderabad, India.
2. Head, Centre for Environment and Climate Change, School of Environmental Sciences,
Jawaharlal Nehru Institute of Advanced Studies, Hyderabad, India.
3. Reader in Chemistry, Department of Chemistry,
St. Ann’s College for Women-Mehedipatnam, Hyderabad, Telangana, India.
Corresponding Author: Dr. D. Sirisha, (Head, Centre for Environment and Climate Change, School of Environmental
Science, Jawaharlal Nehru Institute of Advanced Studies (JNIAS), Hyderabad, Telangana.) & Department of Chemistry,
St. Ann’s College for Women, Hyderabad, Telangan. Email: sirishadavid@gmail.com, gandhigfia2017@gmail.com
Abstract: Green synthesis of nanoparticles using biological molecules derived from the plant sources in the form of
extracts are exhibiting superiority over chemical and physical methods. Development of green nanotechnology is
generating interest of researchers towards eco-friendly green synthesis of nanoparticles. In present study, green synthesis
of stable copper nanoparticles were done using Piper nigrum seed extract. The seed extract prepared by using deionised
water was mixed with 0.01M of copper sulphate (CuSO4.5H2O) solution and an alternative energy source microwave
irradiation used to get nanoparticles in a short incubation period. There is a change in colour (Blue to Green) observed and
that is indicating the formation of copper nanoparticles. The plant based molecules present in the seed extract have highly
controlled assembly, which is suitable for synthesis of nanoparticles. These green synthesised copper nanoparticles were
characterised with the help of Uv-visible spectrophotometer, X-ray diffraction analysis (XRD). It was observed that the
Piper nigrum seed extract can reduce copper ions to copper nanoparticles within 2 to 5 minutes of reaction time. Thus this
method can be used for rapid and eco friendly green synthesis of suitable copper nanoparticles.
Key words: Copper nanoparticles, Green Synthesis, Uv-visible spectrophotometer, X-ray diffraction analysis (XRD),
Piper nigrum seed.
INTRODUCTION
Nanoparticles preparation and study about nanoparticles are importance in the research. The characters of metal
nanoparticles like optical, electrical, magnetic and catalytic are depending on their size, shape and morphology. Several
methods are available which have been extensively used to produce nano sized copper (Figure-1). The micro emulsion
method (Nasser & Husein, 2007), sub merged nanoparticles synthesis system (Kao et al, 2007), flame based aerosol
method (Chiang et al, 2012), sono chemical (Vijaya kumar et al, 2001) hydrothermal (Zhang et al, 2006) and solid state
techniques (Wang et al, 2004) the use of toxic chemicals are subjects of most important concern.
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Figure-1: Various methods of Synthesis of Copper nanoparticles
The use of toxic chemicals for the synthesis of nanoparticles limit their applications. Therefore, development of
clean, biocompatible non toxic and eco friendly method for nanoparticles synthesis deserves merit. The interest in this
field has shifted toward ‘green’ chemistry and bio-processor approach. These approaches focus on utilization of
environmental-friendly, cost-effective and biocompatible reducing agents for synthesis of copper nanoparticles. Here, in
this work we report microwave assisted green synthesis of Copper nanoparticles, reducing the copper ions by the aqueous
extract of Piper nigrum seed, characterized by Uv-Vis spectroscopy, XRD, TGA and DTA.
MATERIALS & METHOD
Materials
Piper nigrum seed (Figure- 2 A) for the biosynthesis of the copper nanoparticles was procured from the local
supermarket. Copper sulphate used in the study is of analytical grade.
Preparation of Sample Extract
5 gm of Piper nigrum seeds were accurately weighed thoroughly washed under running tap water followed by
washing it with double deionised water to remove surface impurities. They were crushed using a blender and finely
macerated. After homogenization 100ml of double deionised water was added and heated over a water bath maintained at
80°C for 15 minutes. The extract obtained was filtered through muslin cloth and then through Whatmann no: 1 Filter
paper (pore size 25µm) and used immediately for the biosynthesis of copper nanoparticles.
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Figure-2 (A): Classification and general information about Piper nigrum
Preparation of Copper Nanoparticles:
For the preparation of 100 ml copper nanoparticles 80 ml of 1mM CuSO4.5H2O solution mixed with 20 ml of
Piper nigrum seed extract and incubated for 3 hours at room temperature. Another set of same experiment conducted with
supply of microwave irradiation (900 waats for 2 minutes) as alternate energy sources. The change in colour indicating the
formation of copper nanoparticles (Figure-2 B).
Piper nigrum seed weighing 5 g were thoroughly washed and dried and crushed into 100 ml sterile distilled water.
Filtered through Whatt man No.1 filter paper (pore size 45 μm) followed by further filtered through 0.22μm sized filters
0.001M Copper Sulphate (CuSO4) was prepared in 1000 ml of distilled water
20 ml of Piper nigrum seed extract was added into 80 ml of aqueous solution of 0.001M Copper Sulphate (CuSO4)
Incubation in microwave oven at 750 watts for 5 minutes
Copper (Cu) nanoparticles formation
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Figure-2 (B): Green synthesis of Copper nanoparticles A) CuSO4 solution B) Piper nigrum seed extract and C)
CuNP solution
Characterisation of Copper Nanoparticles:
The microwave mediated and green synthesized copper nanoparticles were characterized by Uv-
spectrophotometer, XRD, TGA and DTA respectively.
Advantages of using Plant extracts:
1. The generation of nanoparticles utilizing the chemical techniques has been raising worry among the environmentalists as
they have an antagonistic effect on their biology, henceforth the utilization of plant extracts for the arrangement of
nanoparticles is being favored due its salubrious nature towards the earth. Indeed, even in the business it delivers
considerably less harmful waste.
2. The plants supplement both the lessening and also balancing out specialists for the nanoparticles which generally must
be remotely included different strategies.
3. The synthetic strategy is being demonstrated less financially gainful when contrasted with the plant technique as the
support cost is significantly less and the waste transfer requires less exertion among different variables.
4. This technique is shockingly better than utilizing the natural strategy as the support of entire plant framework is
substantially less than a culture of microscopic organisms which needs a horde of marvels to be dealt with.
5. Late investigations have demonstrated that the helpful impacts of plants, from which the nanoparticles are being
determined, can likewise be permeated upon the particles subsequently giving us idealize vehicles to the restorative
materials to follow up on the site of activity and also taking out the need to falsely build up a medication for that specific
affliction
RESULTS & DISCUSSION
Uv-Visible Spectrophotometer
UV-Visible spectroscopy refers to the absorption spectroscopy in the ultraviolet visible spectral region. It uses
light in the visible region and adjacent near infrared (NIR) ranges. In this region of electromagnetic spectrum, molecules
undergo electronic transitions nanoparticles have certain optical properties such as size, shape, concentration,
agglomeration state and refractive index which can be identified by UV-visible spectrometer. Nanoparticles made from
certain metals strongly interact with certain wavelength of light and their unique optical properties leads a phenomena
known as surface Plasmon resonance. In the present study the UV visible spectra for copper nanoparticles synthesized
from Piper nigrum seed extract at different time intervals and different temperatures were represented in Fig-3, Fig-4 and
Fig-5 respectively. The Plasmon resonance produced the peaks at about 300 to 310 nm for copper nanoparticles
synthesized from Piper nigrum seeds extract.
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X-Ray Diffraction Studies
Powder X-ray Diffraction (XRD) is one of the primary techniques used by mineralogists and solid state chemists
to examine the physico-chemical make-up of unknown materials. X-ray diffraction is one of the most important
characterization tools used in solid state chemistry and materials science. XRD is an easy tool to determine the size and the
shape of the unit cell for any compound. Powder Diffraction Methods is useful for Qualitative analysis (Phase
Identification), Quantitative analysis (Lattice parameter determination & Phase fraction analysis) etc. Diffraction pattern
gives information on translational symmetry - size and shape of the unit cell from Peak Positions and information on
electron density inside the unit cell, namely where the atoms are located from Peak Intensities. It also gives information on
deviations from a perfect particle, if size is less than roughly 100 – 200nm, extended defects and micro strain from Peak
Shapes & Widths.
Peak Indexing
Indexing is the process of determining the unit cell dimensions from the peak positions. It is the first step in
diffraction pattern analysis. To index a powder diffraction pattern it is necessary to assign Miller Indices (h k l) to each
peak. Unfortunately it is not just the simple reverse of calculating peak positions from the unit cell dimensions and
wavelength (Cullity 1978).
Figure-6: XRD showing peak indices and 2 θ positions
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XRD analysis of the prepared sample of Copper nanoparticles was done by a Goniometer. Data was taken for the 2θ
range of 20 to 80 degrees with a step of 0.02 degree. Indexing process of powder diffraction pattern was done and Miller
Indices (h k l) to each peak was assigned in first step. Diffractogram of the entire data is in Fig.6.
Table-1: Simple Peak Indexing
Peak position, 2θ 1000 X Sin2 θ 1000XSin2 θ/46 Remark
42.6 132 2.86 12 + 12 + 12 = 3
51.8 188 4.08 22 + 02 + 02 = 4
72.4 359 7.80 22 + 22 + 02 = 8
Indexing has been done in two different methods and data are in Table.1 & Table.2. In table.1, one need to find a
dividing constant and the values in the 3rd column becomes integers (approximately). Here, the constant is 56 (=
188−132). Moreover, the high intense peak for FCC materials is generally (1 1 1) reflection, which is observed in the
sample.
Table-2: Peak indexing from d-spacing
2θ d 1000/d2 (1000/d2)77.32
42.6 2.121 235 3.03
51.4 1.776 282 4.01
73.6 1.286 389 5.03
Table.3: Experimental and standard diffraction angles of Cu specimen
Experimental diffraction angle (2θ in degrees) Standard diffraction angle (2θ in degrees)
JCPDS Copper:04-0836
42.6 43.297
51.4 50.433
73.6 74.130
Three peaks at 2θ values of 42.640, 51.400, and 73.620 deg corresponding to (111), (200), and (220) planes of
copper were observed and compared with the standard powder diffraction card of JCPDS, copper file No. 04–0836.
Table.4 shows the experimentally obtained X-ray diffraction angle and the standard diffraction angle of Cu specimen. The
XRD study confirms / indicates that the resultant particles are (FCC) Copper Nanoparticles.
Particle Size Calculation
From this study, considering the peak at degrees, average particle size has been estimated by using Debye-Scherrer
formula (Nat et al, 2008; Das et al, 2009; Nath et al, 2007; Hall et al, 2000)
Where K is instrument constant (0.9), λ is wavelength of X ray diffraction (0.1541 nm) ‘β’ is FWHM (full width at half
maximum), ‘θ’ is the diffraction angle and ‘D’ is particle diameter size.
1). 2θ = 42.6
2). 2θ = 51.4
3). 2θ = 73.6
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The calculated results were concluding that the microwave mediated green synthesized copper nanoparticles by
using Piper nigrum seeds extract is less than 40 nm in size.
Calculation of d-Spacing
The value of d (the interplanar spacing between the atoms) is calculated using Bragg’s Law: 2dsinθ = nλ
Wavelength λ = 1.5418 Å for Cu
1). 2θ = 42.6
θ = 21.3
2). 2θ = 51.4
θ = 25.7
3). 2θ = 73.6
θ = 36.8
TGA/DTA Analysis
TGA measures the amount of weight change of material either as a function of temperature or isothermally as a
function of temperature in an inert atmosphere of nitrogen. TGA, measured weight loss curve gives information on
changes in sample composition, thermal stability, and kinetic parameters for chemical reactions in the sample. TGA gives
the following characteristics.
1) Thermal stability
2) Material purity
3) Determination of humidity. Is also examines corrosion studies, gasification process and kinetic process. The Figure-7,
TGA curve shows a loss of 50% indicating the loss of water and other volatile components which were used as stabilising
agents. Two exothermic peaks were attributed in this TGA curve. The first weight loss peak was related to the removal of
water from the surface at 250oc and it is due to removal of organic molecules. At higher temperatures no significant
weight loss was observed, there by supporting the crystallinty with high purity. These observations indicate that
phytochemicals present in the Piper nigrum seeds extract serve the dual purpose of reducing the copper salt to nano
dimensional copper particles and it is serving as capping agent.
DTA curve is shown in Figure-8, for green synthesized copper nanoparticle. In this curve two different peaks
were observed and those were representing the exothermic reactions. 1st peak is observed 260oc indicating loss of water
and 2nd peak is observed at 504oc. The initial weight loss from 13.0 mg to 6.5 mg is observed which exactly 50% is. The
area enclosed in the peaks will give the enthalpy of the reaction which is equal to.
Figure-7: TG (%)/DTA spectrum of Copper Nanoparticles
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Figure-8: TG (mg)/DTA spectrum of Copper Nanoparticles
Mechanism of formation CuNPs
The utilization of plant and plant extracts in nanoparticle amalgamation is viewed as profitable over microbial
based framework since it decreases the intricate procedure of keeping up cell cultures. The molecule estimate development
can likewise be controlled by changing combination conditions like pH, reductant focus, temperature, blending proportion
of the reactants and so forth. The plant based combination can be done either extracellularly or intracellularly. Intracellular
blend happens inside the plant though the extracellular union happens in vitro. The examinations uncover that
extracellular blend utilizing plant separates has been viewed as better when contrasted with intracellular amalgamation
(Makarov et al, 2014) on the grounds that it takes out the extraction and decontamination strategies. Biosynthesis of
CuNPs by plant extracts, for example, Eclipta prostrate (Chung et al, 2017), Plantago asiatica leaf extract (Nasrollahzadeh et al,
2017), Ocimum sanctum (Kulkarni & Kulkarni 2013; Bhasker et al, 2014), Camellia sinensis leaf extract (Mohindru & Garg
2017), Gloriosa superba leaf extract (Powar et al, 2016), Carica papaya (Suresh et al, 2014), Mangolia kobus (Lee et al, 2013),
Syzygium aromaticum (Subhankari et al, 2013), Artabotrys odoratissimus (Umesh et al, 2014), Capparis zeylanica (Renganthanan et
al, 2014), Vitis vinifera (Subbaiya et al, 2014), Nerium oleander (Gopinath et al, 2014), Datura metel (Makwana et al, 2014),
Pisidium guajava (Kote et al, 2014), Cinnamum (Koizhaiganova et al, 2014), Aloe vera (Javad et al, 2015), Citrus medica
(Mahendra et al, 2015), Eucalyptus (Pramod et al, 2015), Eupatorium glandulousm (Subbaiya et al, 2015), Cassia fistula (Valli et al,
2015), Phyllanthus embilica (Caroling et al, 2015), Guava (Caroling et al, 2015), Cassia Auriculata (Valli et al, 2016), Ocimum
tenuiflorum (Vemila et al, 2016) and so on have been accounted for. Till date, part of papers has been distributed around
there which portrays the system and part of dynamic biomolecules in blend (Parashav et al, 2011). These examinations
recommended that nearness of phytochemicals in plant separates are the key segment in diminishment and adjustment of
copper particles (Parashav et al, 2011). The phytochemicals which are in charge of lessening are terpenoids, flavonoids,
ketones, aldehydes, amides, and carboxylic acids. The water dissolvable metabolites, for example, flavones, natural acids,
and quinones are exclusively in charge of the bioreduction particles. A few analysts have revealed that a keto-enol progress
of anthraquinone is in charge of arrangement of CuNPs. It has been likewise watched that mesophytes contain three sorts
of benzoquinones: cyperoquinone, dietchequinone, and remirin which may be in charge of lessening of particles and
development of CuNPs. One of the biomolecule which significantly partake is terpenoids. Terpenoids are otherwise called
isoprene, a normally happening natural mixes in plants, they contain five-carbon isoprene units. It has been investigated by
a few specialists that Geranium leaf remove contain terpenoids, which go about as significant player in biosynthesis of
AgNPs (Shanker et al, 2003). Similar results were found with Cinnamomum zeylanicum (cinnamon) removes contains
eugenol which may be in charge of the decrease silver nitrate to AgNPs (Satish kumar et al, 2009).
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Based on previous investigations and information available in the literature, it have been recommended that the
deprotonation of the hydroxyl particle of eugenol prompt arrangement of reverberation balanced out structures which can
additionally oxidized, by reducting metal particles into its nano extend (Satish kumar et al, 2009). Another significant class
of plant metabolite is flavonoids. Falvonoids are gathering of polyphenolic mixes containing 15 carbon particles and are
water dissolvable. Flavonoids can be grouped into: isoflavonoids, bioflavonoids and neoflavonoids, which can go about as
chelating and diminishing operators for metal particles. The practical gathering present in flavonoids are exclusively in
charge of nanoparticle arrangement. The change of flavonoids from the enol to the keto may prompt decrease of metal
particles to frame nanoparticles (Makarov et al, 2014). Ahmad et al. reported that Ocimum basilicum(sweet basil) extract
contains of flavonoids, eugenol and polyphenols that assume enter part in the development of AgNPs from silver particles
by tautomerization of enol to keto shape (Ahmad et al, 2010). A few investigations have been demonstrates that flavonoids
can go about as chelating operators for instance quercetin is a flavonoid which can chelate at three positions including the
carbonyl and hydroxyls at theC3 and C5 positions and the catechol aggregate at the C3' and C4' site (Makarov et al, 2014).
These aides in understanding that flavonoids are associated with start of nanoparticle arrangement (nucleation) and further
accumulation, notwithstanding the bioreduction organize. Glucose a straight monosaccharides having free aldehydic
gathering can specifically go about as decreasing specialists while fructose which contains keto-gathering can go about as
cell reinforcements if tautomeric changes happens from ketone to an aldehyde (Makarov et al, 2014). It has been accounted
for that when glucose was utilized as a diminishing specialist the nanoparticles with various morphologies were watched
while with fructose just monodispersed nanoparticles were watched. It has been hypothesized that aldehydic gathering of
sugar get oxidized into a carboxylic gathering by means of the nucleophilic expansion of OH-, which eventually prompt
decrease of metal particles and blend of nanoparticles (Makarov et al, 2014).
There are three principle stages which incorporated into the plant interceded amalgamation. Beginning stage
otherwise called enactment stage amid which metal particles get lessened and the decreased metal iotas get nucleated;
another is the development stage in which unconstrained accumulation of little contiguous nanoparticles jumps out at
frame particles of a bigger measurement, which are thermodynamically more steady; last stage is the end stage which
decides the last state of the nanoparticles (Makarov et al, 2014; Si & Mandal 2007). Increment in the development stage,
prompt total of nanoparticles into nanotubes, nanorods and nanotriangles and so on (Makarov et al, 2014).. In the end
stage, nanoparticles experience conformational change which is thermodynamically steady, which affirms the part of plant
concentrate to settle metal nanoparticles.
CONCLUSION
The present examination speaks to a clean, non-dangerous and in addition eco-accommodating technique for
combining CuNPs. The topping around every molecule gives normal substance condition framed by the bio-natural
compound present in the Piper nigrum seed extract, which might be mainly in charge of the particles to wind up plainly
balanced out. This method gives us a straightforward and proficient route for the combination of nanoparticles with
tunable optical properties administered by molecule measure. From the of nanotechnology perspective, this is a critical
improvement for incorporating CuNPs financially. All in all, this green science approach toward the combination of
CuNPs has a few focal points viz, simple process by which this might be scaled up, financial suitability, and so forth.
ACKNOWLEDGEMENTS
The authors are grateful to Dr. Shilpa Chakra, Head of the Department, Centre for Nano Science and
Technology, Institute of Science and Technology, Jawaharlal Nehru Technological University Hyderabad, Kukatpally,
Telanagana for providing instruments for the characterisation of green synthesized Copper Nanoparticles.
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Reference
1. A. Bhaskar, A. RajaLakshmi, N. Krithiga, S. Gurupavithra, A. Jayachitra (2014) “biosynthesis of copper Nanoparticles using ocimum
sanctum leaf extract and its antimicrobial property”, international journal of biological and pharmaceutical research.5(6), pp.511-515.
2. B. D. Hall, D. Zanchet and D. Ugarte (2000) Estimating nanoparticle size from diffraction measurements , Journal of Applied
Crystallography, Volume 33, Part 6 pp. 456- 467.
3. B. Mussabayeva, K. Murzagulova, Z. Kasymova, Z. Aripzhanova, L. Orazzhanova, M. Koizhaiganova (2014). “Preparation of silver and
copper Nanoparticles in plant extracts”, Bothalia journal, vol 44, no.12 pp.558-568.
4. B.A. Makwana, P. Parikh, D. Zala, (2014) “biosynthesis of copper Nanoparticles and their antimicrobial activity” oALib preprints DOI:
10.4236/oalib.preprints.1200067.
5. B.D. Cullity, “Elements of X-ray Diffraction”, Addison-Wesley Pub.Co., (1978).
6. C.Y. Chiang, K. Aroh, S. Ehrman (2012), copper oxide nanoparticle made by flame spray pyrolysis for photoelectrochemical water splitting e
part 1. CuO nanoparticle preparation. Int J Hydrogen energy 37, pp.4871-4879.
7. G. Caroling, E. Vinodhini, A.M. Ranjitham, P. Shanthi (2015) “biosynthesis of copper Nanoparticles using aqueous phyllanthus embilica
(Gooseberry) extract-characterization and study of antimicrobial effects”, International Journal of Nanomaterials and Chemistry, 1, no.2,
pp.53-63.
8. G. Caroling, M. Nithya, M. Priyadharshini, E. Vinodhini, A.M. Ranjitham, P. Shanthi (2015) “biosynthesis of copper Nanoparticles
using aqueous guava extract-characterization and study of antibacterial effects”, Int. J. Pharm.Bio.Sic. vol 5 issue 2, pp25-43.
9. G. Valli, M. Suganya, (2015) “green synthesis of copper Nanoparticles using cassia fistula flower extract”, J. Bio. Innov 4 (5), pp: 162-170.
10. G. Valli, S. Geetha (2016) “green synthesis of copper Nanoparticles using cassia Auriculata leaves extract”, International journal of
technochem. Research, 2(1), pp 05-10.
11. H. Lee, S.K. Beom, Y.J. song (2013) “biological synthesis of copper Nanoparticles using Magnolia kobus leaf extract and their antibacterial
activity”, Journal chem.. Technol Biotechnol ; 88, pp.1971-1977.
12. I. Subhankari, P.L. Nayak (2013) “synthesis of copper Nanoparticles using Syzygium Aromaticum(cloves) aqueous extract by using green
chemistry” , world journal of nano science and technology 2(1), pp.14-17.
13. J. Karimi, S. Mohsenzadeh (2015) “Rapid, green and ecofriendly biosynthesis of copper Nanoparticles using flower extract of aloevera”.
Synthesis and Reactivity in Inorganic, Metal-Organic, and Nano-Metal Chemistry, 45, pp.895–898.
14. J. Wang, J. Yang, S. Jinquan, B. Ying (2004), Synthesis of copper oxide nanomaterials and the growth mechanism of copper oxide nanorods.
Mater Des 25 (7), pp.625-629.
15. J.K.V. Mahavinod angrasan, R. Subbaiya (2014)“biosynthesis of copper Nanoparticles by vitis vinifera leaf aqueous extract and its
antibacterial activity”, International journal of current microbiology and applied sciences 3(9), pp.768-774.
16. K. Saranyaadevi, V. Subha, E.R.S. Ravindran, S. Renganathan (2014) “synthesis and characterization of copper Nanoparticles using
Capparis Zeylanica leaf extract”, international Journal of chemtech research, 6(10), pp.4533-4541.
17. M. Gopinath, R. Subaiya, M. Masilamani selvam, D. Suresh (2014) “synthesis of copper Nanoparticles from Nerium oleander leaf aqueous
extract and its antibacterial activity”, International Journal of Current Microbiology and Applied sciences,3(9) pp.814-818.
18. M. Rai, S. Shende, P. Avinash Ingle, A. Gade (2015) “green synthesis of copper Nanoparticles by Citrus medical inn.(Idilimbu) juice and its
antimicrobial activity”, world j microbial biotechnol31:865-873.
19. M. Sathishkumar, K. Sneha, S.W. Won, C.W. Cho, S. Kim (2009) Cinnamon zeylanicum bark extract and powder mediated green
synthesis of nanocrystalline silver particles and its bactericidal activity. Colloids Surf B Biointerfaces 73, pp.332-338
20. M.J. Kao, C.H. Lo, T.T. Tsung, Y.Y. Wu, C.S. Jwo, H.M. Lin (2007), copper-oxide brake nanofluid manufactured using arc-submerged
nanoparticle synthesis system. J Alloy compd 434-436, pp. 672-674.
21. N.Ahmad, S. Sharma, M.K. Alam, V.N. Singh, S.F. Shamsi, (2010) Rapid synthesis of silver nanoparticles using dried medicinal plant of
basil. Colloids Surf B Biointerfaces 81, pp. 81-86.
22. N.N. Nasser, M.M. Husein (2007), Effect of microemulsion variables on copper oxide nanoparticle uptake by AOT microemulsions. J colloid
Interf sci 316, pp.442-450.
23. P. Kulkarni, V.D. Kulkarni, S. Suryawanshi (2015) “biosynthesis of copper nanoparticle using aqueous extract of Eucalyptus sp. Plant
leaves extract”, vol. 109, no. 2, pp.45-56 .
IAETSD JOURNAL FOR ADVANCED RESEARCH IN APPLIED SCIENCES
VOLUME 5, ISSUE 2, FEB/2018
ISSN NO: 2394-8442
http://iaetsdjaras.org/869
24. R. Das, S.S. Nath, D. Chakdar, G. Gope and R. Bhattacharjee (2009) Preparation of Silver Nanoparticles and Their Characterization ,
Journal of nanotechnology online, (DOI : 10.2240/azojono0129).
25. R. Subbaiya, M.M. selvam, (2015) “Synthesis and characterization of copper Nanoparticles using Eupatorium glandulosum extract and their
antimicrobial, antioxidant activities”, Research journal of pharmaceutical, biological and chemical sciences, 6 (2), pp.1117 – 1127.
26. R. Vijayakumar, R. Elgamiel, Y. Diamant, A. Gedanken (2001) Sonochemical preparation and characterization of nanocrystal-line copper
oxide embedded in ploy (polyvinyl) and its effect on crystal growth of copper oxide. Langmuir. 17, pp.1406-1410.
27. R.J. Kote, M.R. Mulani, S.A. Kadam, M.B. Solankar (2014) “Anti-mycobacterial activity of Nanoparticles from Psidium guajava L.”,
Journal of microbiology and Biotechnology Research. 4(5), pp.14-17.
28. S. Si, T.K. Mandal (2007) Tryptophan-based peptides to synthesize gold and silver nanoparticles: a mechanistic and kinetic study. Chem Eur
J 13, pp.3160- 3168.
29. S. Vennila, T. Nithya, (2016) “green synthesis of copper, silver Nanoparticles using ocimum Tenuiflorum leaf extract”, world journal of
pharmaceutical research, vol. 5,issue 1, pp.257-265.
30. S.S. Nath, D. Chakdar, G. Gope, (2007) Synthesis of CdS and ZnS quantum dots and their applications in electronics, Nanotrends- A
journal of nanotechnology and its application, 2(3), pp. 212-220.
31. S.S. Nath, D. Chakdar, G. Gope, D.K. Avasthi (2008) Effect of 100 Mev Nickel Ions on Silica Coated ZnS Quantum Dot, Journal of
Nanoelectronics and Optoelectronics, 3, pp.1-4.
32. S.S. Shankar, A. Ahmad, M. Sastry (2003) Geranium leaf assisted biosynthesis of silver nanoparticles. Biotechnol Prog 19, pp.1627-1631.
33. U. Kathad, H.P. Gajera (2014) “synthesis of copper Nanoparticles by two different methods and size comparision”, international Journal of
pharma and Bio Sciences, 5(3), pp.533-540.
34. U.K. Parashar, V. Kumar, T. Bera, P.S. Saxena, G. Nath (2011) Study of mechanism of enhanced antibacterial activity by green synthesis
of silver nanoparticles. Nanotechnol 22, pp. 1-13.
35. V.V. Makarov, A.J. Love, O.V. Sinitsyna, S.S. Makarova, I.V. Yaminsky, et al. (2014) "Green" nanotechnologies: Synthesis of metal
nanoparticles using plants. Acta naturae 6, pp.35-43.
36. Y. Zhang, S. Wang, X. Li, L. Chen, Y. Qian, Z. Zhang (2006) CuO shuttle like nanocrystals synthesized by oriented attachment. J Cryst
Growth 291 (1), pp.196-201.
IAETSD JOURNAL FOR ADVANCED RESEARCH IN APPLIED SCIENCES
VOLUME 5, ISSUE 2, FEB/2018
ISSN NO: 2394-8442
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