8/8/2019 Lactic Acid Bacteria Antimicrobial Activity Against MRSA

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Lactic Acid Bacteria as an Antibacterial Against Methicillin-ResistantStaphylococcus aureus

Ashley Morisako— Department of Biology, Santa Clara University, Santa Clara, California

MATERIALS & METHODS

MATERIALS & METHODS RESULTS

RESULTS

RESULTS

RESULTSINTRODUCTION

INTRODUCTION

CONCLUSIONS

CONCLUSIONSLITERATURE CITED

LITERATURE CITED

-Methicillin resistant  Staphylococcus aureus ( )MRSA- ,is a multi drug resistant opportunistic bacteria

.known worldwide MRSA cultures are prominent in

 hospitals where antibiotics are given to.patients for treatment The antibiotics disrupt. -the natural microbial flora in the body Non

( )pathogenic lactic acid bacteria LAB provide.some promising solutions LAB strains produce

(bacteriocins and fermenting products organic, )acids hydrogen peroxide to help control MRSA growth  in vitro . Bacteriocins are small peptides

.synthesized by the ribosomesthat act as toxins,For synthesis an induction factor needs to

( )bind to the histidine kinase receptor for.signal transduction as shown in the figure below

 Lactobacillus acidophilus   and  Lactobacillus casei  were used to test their antimicrobial

.activity against MRSA clinical isolates All tests were done by agar diffusion method on/ . -solid liquid medium Pre culture preparation was

 done to subculture the LAB for antimicrobial activity and grown in PeptonizedMilk Nutrient( ).PMN LAB colonies were grown as spots on MRS

.agar MRSA colonies were inoculated in BHI broth.and spread on the same MRS agar Plates were°incubated for 24 to 48 hours at 37 C

 Different commercial food products fermented b y L AB w er e t es te d f or a nt im ic ro bi al

.activity against MRSA

 Single and mixed LAB strains produce powerful antimicrobial products that

.inhibit the growth of MRSA In a natural,s et ti ng m ix ed c ul tu re s tu di es c an

 accurately depict the microbial population

.that lives within us Giving patients antibiotics increases the risk for the adaptation of antibacterial resistant

.species The bacteriocins and fermented products of lactic acid bacteria provide a

 way to inhibit the growth of antibacterial resistant .Staphylococcus aureus   Further r ese ar ch m us t b e d on e t o t es t t he

 effectiveness of food additives that can s ti mu la te t he g ro wt h o f l ac ti c a ci d bacteria to inhibit MRSA naturally  in vivo .

- , .Karska Wysocki B et al Antibacterial activity of Lactobacillus acidophilus   and  Lactobacillus casei 

-against methicillin resistant  Staphylococcus aureus  ( ). ; ( ),MRSA Microbiol Res 2006 2010

: . / . . . .doi 110 1016 j micres 2009 11 008

, , . .Nes I et al 1996 Biosynthesis of bacteriocins in.lactic acid bacteria  Antonie van Leeuwenhoek  ( ):70

- .113 128

, , . .Shnayerson Michael and Mark J Plotkin The Killers : - Within the Deadly Rise of Drug resistant Bacteria .: , , . .Boston Little Brown and 2002 Print

ACKNOWLEDGEMENTS

ACKNOWLEDGEMENTS

. , ,T Ruscetti Department of Biology Santa Clara,University California. -Figure 2 MRSA strains against LAB from food milk

 based food products had the greatest inhibition. .diameter Error bars represent standard deviation . ( )Figure 3 Who will win? MRSA left or .L 

 acidophilius  ( ).right:// . - . . / / / / - - - .http www musee afrappier qc ca images site large lactobacillus acidophilus02 milos kalab jpg