Label the parts on your microscope picture. Eyepiece/ocular lens

magnification = 10X

Label the parts on your microscope picture. Eyepiece/ocular lens

magnification = 10X

Arm-Support

Label the parts on your microscope picture. Eyepiece/ocular lens

magnification = 10X

Arm-Support

Stage - support

Label the parts on your microscope picture. Eyepiece/ocular lens

magnification = 10X

Arm-Support

Stage - support

Coarse adjust – general focus

Label the parts on your microscope picture. Eyepiece/ocular lens

magnification = 10X

Arm-Support

Stage - support

Coarse adjust – general focusFine Focus – high power focus

Label the parts on your microscope picture. Eyepiece/ocular lens

magnification = 10X

Arm-Support

Stage - support

Coarse adjust – general focusFine Focus – high power focus

Base - support

Label the parts on your microscope picture. Eyepiece/ocular lens

magnification = 10X

Arm-Support

Stage - support

Coarse adjust – general focusFine Focus – high power focus

Base - support

Light source

Label the parts on your microscope picture. Eyepiece/ocular lens

magnification = 10X

Arm-Support

Stage - support

Coarse adjust – general focusFine Focus – high power focus

Base - support

Light source

Diaphragm – adjusts amount of light

Label the parts on your microscope picture. Eyepiece/ocular lens

magnification = 10X

Arm-Support

Stage - support

Coarse adjust – general focusFine Focus – high power focus

Base - support

Light source

Diaphragm – adjusts amount of light

Stage clips – holds slide

Label the parts on your microscope picture. Eyepiece/ocular lens

magnification = 10X

Arm-Support

Stage - support

Coarse adjust – general focusFine Focus – high power focus

Base - support

Light source

Diaphragm – adjusts amount of light

Stage clips – holds slide

Objective Lenses Scanning = 4X

Low Power = 10X

High power = 40X

Label the parts on your microscope picture. Eyepiece/ocular lens

magnification = 10X

Arm-Support

Stage - support

Coarse adjust – general focusFine Focus – high power focus

Base - support

Light source

Diaphragm – adjusts amount of light

Stage clips – holds slide

Objective Lenses Scanning = 4X

Low Power = 10X

High power = 40X

Revolving nosepiece

Label the parts on your microscope picture. Eyepiece/ocular lens

magnification = 10X

Arm-Support

Stage - support

Coarse adjust – general focusFine Focus – high power focus

Base - support

Light source

Diaphragm – adjusts amount of light

Stage clips – holds slide

Objective Lenses Scanning = 4X

Low Power = 10X

High power = 40X

Revolving nosepiece

Magnification of a Compound Microscope•Because you are looking through multiple lenses the lenses have a “compounding” effect.

•The eyepiece always magnifies 10X

  Eyepiece Objective Lens Magnification

Scanning lens (red)

 10X

   

Low Power (yellow) 

10X   

High Power (blue)

 

10X    

Magnification of a Compound Microscope•Each of the objective lenses have their own magnification

  Eyepiece Objective Lens Magnification

Scanning lens (red)

 10X

 

4X 

Low Power (yellow) 

10X 

10X 

High Power (blue)

 

10X   40X  

Magnification of a Compound Microscope•You then multiply the eyepiece with the objective lens to determine the total magnification

  Eyepiece Objective Lens Magnification

Scanning lens (red)

 10X

 

4X 

Low Power (yellow) 

10X 

10X 

100XHigh Power (blue)

 

10X 

40X 

400X

40X

General Procedures

•·  Make sure all backpacks are out of the aisles before you get a microscope! Always carry the microscope with one hand on the Arm and one hand on the Base.

•·  Wear your glasses, the microscope will focus to your eyesight!

•·  Keep both eyes open, your brain will learn to ignore the other eye.

Focusing a Specimen

• Always start on low power or scanning.

Focusing a Specimen

• Always start on low power or scanning.• Focus the specimen using the course

adjust.

Focusing a Specimen

• Always start on low power or scanning.• Focus the specimen using the course

adjust.• Re-center. If high power is needed, turn

only after re-centering!

Focusing a Specimen

• Always start on low power or scanning.• Focus the specimen using the course adjust.• Re-center. If high power is needed, turn only

after re-centering!• Now use the FINE FOCUS ONLY for your final

adjustments.

Care of microscopes

• A. You must always keep the microscope clean and dry.

B. When a slide is focused under the high power objective, it should not be pulled or removed.

C. The microscope lenses must be cleaned by lens paper after each time using the microscope. Xylene can be used as a solvent for erasing the dusts of the lenses.

Care of microscopes• D. When you are going to view specimens first view under the

low power objective lens, then bring the specimen into the focus by coarse adjustment screw. After that if you want to get a more magnified view then rotate the nose piece to the high power objective lens and then bring the specimen into focus by using the fine adjustment screw.

E. Avoid lowering the objective lens into the slide, especially if there is not a coverslip. Even when using a coverslip, be careful and mindful of you raising the slide to far up and crushing the coverslip and damaging both the slide and microscope.

Care of microscopes

• F. Never remove the slide from the stage when it is focused under the high power objective.

G. When the use is over, clean all lenses with lens paper and carefully (with two hands) replace microscope on shelf.

Care of microscopes

• Always carry microscope with two hands.• Store microscope with scan strength (lowest

power) objective in place.