Post on 10-Feb-2021
Green synthesis and characterisation of
Anti-Cancer drug carrier, Fe3O4-Ag core-
shell magnetic nanoparticles, supported on
graphene oxide
School of Psychological and Clinical Sciences
Dr Vinuthaa Murthy, Dr Roshanak Khandanlou
& Dr Martin Boland
Nanomedicine• Great potential for early detection, accurate
diagnosis, and treatment of cancer• 10 to 100nm - 1000 to 10,000 times smaller
than human cells• smaller than large biological molecules such as
enzymes, receptors, and antibodies
• Promise for a “magic gold bullet” against cancer
Green synthesis and characterisation of Anti-Cancer drug carrier nanoparticles
Synthesis of Nano particles
Some Plant productsNatural Polyphenols - rich in flavonoids, phenolic acids
and tannins.
Inhibitory effect in carcinogenesis and tumor growth
Parameter optimisation pH
Reaction time
Leaf boiling time.
UV-visible spectra of NPs prepared at different
(a) pH (b) reaction time, and (c) leaf boiling time.
Characterization of Nanoparticles
XRD pattern of purified NPs synthesized
using leaf extract.
TEM micrograph at different magnifications
SAED pattern
particles size distribution Size:Ranging from 3.56 to 24.5 nm
Inhibitory Concentration (IC50)
In vitro anticancer activity MTT Cell Proliferation Assay
Cell lines Human breast cancer (MCF-7)liver cancer (HepG2)human dermal fibroblast (HDF-7)
In vitro anticancer activity MTT Cell Proliferation Assay
MCF-7 : at 500 µgNPs 97.4% Leaf extract 87.9%
HepG2 :at 500 µgNPs 97.4% Leaf extract 99.2%
at 250 µgNPs 91.7% Leaf extract 74.4%
at 250 µgNPs 91.7% Leaf extract 74.4%
Conclusion
• Proof-of-principle study
• A novel green method for the synthesis of stablecolloidal NPs using leaf extract
• The synthesised NPs have spherical or near sphericalshape with an average size of 8.40 ± 0.084 nm.
• In-vitro anticancer show NPs induced significantcytotoxicity effect against MCF-7 and HepG2 cancercells in a dose dependent manner and with nosignificant cytotoxicity on normal HDF-7 cells.
Thank you
AcknowledgementsNMIMS Mumbai IndiaProf Dhananjaya Saranath Ms Hetal DamaniCentral Analytical Research Facility (CARF) Institute for Future Environments, Queensland University of Technology (QUT)
Continuing work…