Green synthesis and characterisation of

Anti-Cancer drug carrier, Fe3O4-Ag core-

shell magnetic nanoparticles, supported on

graphene oxide

School of Psychological and Clinical Sciences

Dr Vinuthaa Murthy, Dr Roshanak Khandanlou

& Dr Martin Boland

Nanomedicine• Great potential for early detection, accurate

diagnosis, and treatment of cancer• 10 to 100nm - 1000 to 10,000 times smaller

than human cells• smaller than large biological molecules such as

enzymes, receptors, and antibodies

• Promise for a “magic gold bullet” against cancer

Green synthesis and characterisation of Anti-Cancer drug carrier nanoparticles

Synthesis of Nano particles

Some Plant productsNatural Polyphenols - rich in flavonoids, phenolic acids

and tannins.

Inhibitory effect in carcinogenesis and tumor growth

Parameter optimisation pH

Reaction time

Leaf boiling time.

UV-visible spectra of NPs prepared at different

(a) pH (b) reaction time, and (c) leaf boiling time.

Characterization of Nanoparticles

XRD pattern of purified NPs synthesized

using leaf extract.

TEM micrograph at different magnifications

SAED pattern

particles size distribution Size:Ranging from 3.56 to 24.5 nm

Inhibitory Concentration (IC50)

In vitro anticancer activity MTT Cell Proliferation Assay

Cell lines Human breast cancer (MCF-7)liver cancer (HepG2)human dermal fibroblast (HDF-7)

In vitro anticancer activity MTT Cell Proliferation Assay

MCF-7 : at 500 µgNPs 97.4% Leaf extract 87.9%

HepG2 :at 500 µgNPs 97.4% Leaf extract 99.2%

at 250 µgNPs 91.7% Leaf extract 74.4%

at 250 µgNPs 91.7% Leaf extract 74.4%

Conclusion

• Proof-of-principle study

• A novel green method for the synthesis of stablecolloidal NPs using leaf extract

• The synthesised NPs have spherical or near sphericalshape with an average size of 8.40 ± 0.084 nm.

• In-vitro anticancer show NPs induced significantcytotoxicity effect against MCF-7 and HepG2 cancercells in a dose dependent manner and with nosignificant cytotoxicity on normal HDF-7 cells.

Thank you

AcknowledgementsNMIMS Mumbai IndiaProf Dhananjaya Saranath Ms Hetal DamaniCentral Analytical Research Facility (CARF) Institute for Future Environments, Queensland University of Technology (QUT)

Continuing work…