GeneOntology(GO)EnrichmentInapreviousexerciseyou identifiedall gene inCryptosporidium species that containawiththeYXXΦreceptorsignalmotif:http://cryptodb.org/cryptodb/im.do?s=928309b4c1b9ef3f

LookattheresultsforC.muris.Areyouabletoidentifytypesofproteinsthatareenrichedinthelistjustbylooking?Howconfidentareyouaboutyourconclusion?Tohelp,youcanalsoclickonthegraphiconnexttotheproductdescriptioncolumntogenerateawordcloudofallthewordsintheproductdescriptionsofyourresults.Isthisstatistical?

Enrichmentanalysisallowsyoutolookforoverrepresentedfeaturesinyourresults.GOtermsprovideastructuredvocabularyforproductdescriptions.AtEuPathDBwegetGOtermsfromtheofficialannotationandbytransferringGOtermsassociatedwithInterProdomains(usingInterProscan).Herearesomeusefullinkswithadditionalinformation:http://geneontology.org/page/documentationhttp://geneontology.org/page/go-enrichment-analysis

TorunaGOenrichmentanalysis,clickonthe“AnalyzeResults”tabthenclickontheGOenrichmentoption:

Nextselect“thecellularcomponent”optionandsubmityouranalysis:

Examineyourresults.Dotheymakesense?

SomemoreinfoonFischer’sexacttest:http://udel.edu/~mcdonald/statfishers.htmlSomemoreinfoaboutOddsrations:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2938757/SomemoreinfoonfalsediscoveryratesandPvaluecorrection:http://brainder.org/2011/09/05/fdr-corrected-fdr-adjusted-p-values/