Gene Ontology (GO) Enrichment - EuPathDB · 2020. 10. 23. · Enrichment analysis allows you to...
Transcript of Gene Ontology (GO) Enrichment - EuPathDB · 2020. 10. 23. · Enrichment analysis allows you to...
GeneOntology(GO)EnrichmentInapreviousexerciseyou identifiedall gene inCryptosporidium species that containawiththeYXXΦreceptorsignalmotif:http://cryptodb.org/cryptodb/im.do?s=928309b4c1b9ef3f
LookattheresultsforC.muris.Areyouabletoidentifytypesofproteinsthatareenrichedinthelistjustbylooking?Howconfidentareyouaboutyourconclusion?Tohelp,youcanalsoclickonthegraphiconnexttotheproductdescriptioncolumntogenerateawordcloudofallthewordsintheproductdescriptionsofyourresults.Isthisstatistical?
Enrichmentanalysisallowsyoutolookforoverrepresentedfeaturesinyourresults.GOtermsprovideastructuredvocabularyforproductdescriptions.AtEuPathDBwegetGOtermsfromtheofficialannotationandbytransferringGOtermsassociatedwithInterProdomains(usingInterProscan).Herearesomeusefullinkswithadditionalinformation:http://geneontology.org/page/documentationhttp://geneontology.org/page/go-enrichment-analysis
TorunaGOenrichmentanalysis,clickonthe“AnalyzeResults”tabthenclickontheGOenrichmentoption:
Nextselect“thecellularcomponent”optionandsubmityouranalysis:
Examineyourresults.Dotheymakesense?
SomemoreinfoonFischer’sexacttest:http://udel.edu/~mcdonald/statfishers.htmlSomemoreinfoaboutOddsrations:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2938757/SomemoreinfoonfalsediscoveryratesandPvaluecorrection:http://brainder.org/2011/09/05/fdr-corrected-fdr-adjusted-p-values/