BMEN 423/621Laser Microscopy

Alvin T. Yeh

ayeh@tamu.edu

http://biomed.tamu.edu/tml

K. Konig J. Microsc. 200: 83-104 (2000).

Microscopic ImagingLight- vs. Dark-field imaging

Stained vs. unlabeledVital vs. fixed

Increasing use of Laser Microscopy

W.R. Zipfel, R.M. Williams, W.W. Webb Nat. Biotechnol. 21(11): 1369-1377 (2003).

•commercialization of laser microscopy systems•increased technical savvy of biologists

•benefit to biomedical studies

Technically Demanding

W.R. Zipfel, R.M. Williams, W.W. Webb Nat. Biotechnol. 21(11): 1369-1377 (2003).

Laser-Tissue Interaction

K. Konig J. Microsc. 200: 83-104 (2000).

Does this schematic capture photophysics correctly?

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