Biacore Molecular Interactions Shared Resource (BMISR)

Aykut Uren, MD, Directorau26@georgetown.edu

BMISR

• Have you ever wondered if two molecules directly interact with each other ?

• If they do, how strong is that interaction?

• Were you frustrated because you didn’t have the necessary technology or expertise to make it happen?

What can you use?

• Proteins

• DNA

• RNA

• Small molecules

What can you do?

DetectBinding Yes/No

IdentifySpecificity

Binding Partners

CharacterizeAffinityKinetics

ConcentrationThermodynamics

Biacore T-100

Integrated MicrofluidicsGold Sensor Chip

SPR Detection System

Biacore T-100

Real TimeSee binding as it happens

•Unique binding information (on- and off-rates) for any size of biomolecule•Study weak or fast interactions

Label Free•No labels required•Measure binding properties of unmodified substances •Reduce time and workload

Contract Free•Non-invasive•Measure opaque or colored samples

Sample Applications

(Chang)

Protein DNA interactions:• DNA repair enzymes binding to DNA• Transcription factors binding to DNA

Small Molecule Protein Interactions:• Small molecule inhibitors of enzymatic activities• Small molecule inhibitors of protein-protein interactions

Protein peptide interactions

Protein RNA interactions

Shared Resources are partially supported by the Georgetown Howard University Center for Clinical and Translational Studies (GHUCCTS) 1UL1RR031975-01

In your publications, remember to mention support provided from the GHUCCTS.