Post on 17-Oct-2019
1
2010 12 15
( )
, ( , PWRI)Andrew JOHNSON
(Centre for Ecology and Hydrology, CEH)
22
•
2005 2009
•
• Roach
EDC
2
0 per cent
1–20 per cent
21–40 per cent
41–60 per cent
61–80 per cent
81–100 per cent
Jobling et al., 2006)
3
17�-estradiol (E2)
Estrone (E1)
OH
OH
O
OH
Ethynylestradiol EE2)
OH
OH
ng/L4
0
2
4
6
8
10
12
14
16
18
England Japan
No
rma
lis
ed
ex
cre
tio
n E
2 e
qu
iv u
g/h
ea
d/d
2%( , 2001)
%
E2 (Johnson et al., 2006
0.6 %
?
? ? ?
6
1)2)3)
UK-J Phase 2 CORE 1 (2005 to 2009)
Estradiol (E2) Estriol (E3) Estrone(E1)
( or
Ethynyl estradiol (EE2)
O
C ONa
OH
HO
OH
O
OHSO
O
OH
OH
7
Sample
SPE
(GF/B)
Oasis HLB
STD
NH2 Cartridge
CH3CN/H2O(1:9),1ml
MeOH 0.5% NH4OH/MeOH
N2 Evap.
LC-MS/MS LC-MS/MS
Clean up
Clean-up with NH2
Change LC 50 mm 100 mm
: 500 ml
/ : 1000 ml
8
0
10
20
30
40
50
60
70
80
90
100
E1 E2 E3 EE2 E1-3S E2-3S E3-3S E1-3G E2-3G E3-3G
Con
cent
ratio
n (n
g/L)
Influent Effluent
UK (n = 3)
� E1, E2 EE2
�
�
=Range
9
� EE2
� E1� E1
n=29
0
10
20
30
40
50
60
70
80
90
100
E1 E2 E3 E1-3S E2-3S E3-3S E1-3G E2-3G
Con
cent
ratio
n (n
g/L)
0
10
20
30
40
50
60
70
80
90
100
Rem
oval
effi
cien
cy (
%)
Influent Effluent Removal
10
0
20
40
60
80
100
E1 E2 E3 EE2 E1-3S E2-3S E3-3S E1-3G E2-3G E3-3G
Rem
oval efficien
cy (%
)
Removal efficiency
-80
-60
-40
-20
0
20
40
60
80
100
E1 E2 E3 EE2 E1-3S E2-3S E3-3S E1-3G E2-3G E3-3G
Rem
oval
effi
cien
cy (%
)E2,EE2
E1
Jap
an
(n
= 9
)U
K (
n=
3)
=Range
11
12
� E1 EE2
E2
� EE2
0
10
20
30
40
50
Con
cent
ratio
n (n
g/L)
Influent
Effluent
Downstream
E1 E2 E3 EE2 E1-3S E2-3S E3-3S E2-3&17GE3-3&17G
Co
nc
en
tra
tio
n (n
g/L
)
13
Benson Sandhurst
R. Thames Camberley
Parking
Sandhurst
14
E2
?
PST Reactor FST
Qs
Qs Qs
PST Reactor FST
Qs
Qs
kQs
Qs
kQs
+
SRT
15
SRT=22E1 E2
�� ..
N=6
0
2
4
6
8
10
12
14
16
18
20
E1 E2 E3 E1-3S E2-3S E3-3S
Con
cent
ratio
n (n
g/L)
0
20
40
60
80
100
Rem
oval
effi
cien
cy (
%)
Secondary Effluent After Ozonation Removal
16
D7
D4
D5
D3
D2
D9
D8D6
D1
U2
U1
K1
U9 U6
U7
U9
U10
K7
K9K8
K6
K5
K10
K4
K2
K3
U3 U4
U9
17
�E2 EE2 , E1
�E1 ,
�E1
(segment)
(segment)
STP
STP
STP
60
, , ,
18
0
1
2
3
4
5
6
7
8
9
10
Fro
me
Dar
t
Tor
ridge
Cuc
kmer
e
Gre
at S
tour
Aru
n
Soa
r
Tem
e
Cro
uch
Cam
Wea
r
Tee
s
Ede
n
Cal
der
Wyr
e
Taf
f
Tyw
i
Eas
tern
Cle
ddau
Con
wy
Mer
sey
Irwel
l
Aire
Don
Bly
th
Ere
was
h
Tre
nt
Idle
Littl
e O
use
Wav
eney
Tha
mes
Med
way
Avo
n
0
1
2
3
4
5
6
7
8
9
1075% 25%
19
EEQ (ng/L) E
sti
mate
d E
EQ
E2-e
qu
ivale
nt,
ng
/L
ng/L
Risk Class
No risk
At risk
High risk
LF2000-WQX
(>1 ng/L EEQ)
(>10 ng/L EEQ)20
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5
5.0R
umoi
Sar
u
Tok
oro
Ishi
kari
Aka
Yon
esiro
Kita
kam
i
Tsu
rum
i
Kuj
i
Nak
a
Kak
ehas
hi
Kur
obe
Ara
Shi
nano
Kik
u
Abe
Syo
unai
Kis
o
Kita
Yam
ato
Yur
a
Ash
ida
Asa
hi
Gou
no
Dok
i
Mon
obe
Niy
odo
Yos
hino
Hom
myo
Kim
otsu
ki
Ooi
ta
Chi
kugo
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
4.5
5.0
1ng/L
30.6 18.0
98Summer
98Autumn
99Spring
99Summer
99Autumn
99Winter
00Autumn
01Autumn
NA NA NA NA NA
NA:No available data
21
Estr
og
en
ic A
cti
vit
ies, E
2-e
qu
ivale
nt,
ng
/LEEQ
(ng/L)
YES Assayw/ hER
22
•• k=0.067 /h
0
1
2
3
4
0 1 2 3 4
Estimated Concentration (ng/L)
Mea
sure
d C
once
ntra
tion
(ng/
L)
Theoretical line (x=y)
23
24
� SRT E1 , O3.
25
SRT + O3
E1
25
• EE2E2
• SRT
•
•
•
Summary
26
27
Acknowledgements
12th Annual Scientific Workshop UK-Japan Co-operation for Research on Endocrine Disrupters in the Aquatic Environment held at Otterburn HallNorthumberlandNational Park UK on 2-4 November 2010