BcBtB B¨BmB®6 B1 ê 4B+ G - env.go.jp · PDF file0 per cent 1 – 20 per cent...

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  • 1

    2010 12 15

    ( ) , ( , PWRI) Andrew JOHNSON

    (Centre for Ecology and Hydrology, CEH)

    22

  • 2005 2009 •

    • Roach

    EDC

    2

  • 0 per cent

    1–20 per cent

    21–40 per cent

    41–60 per cent

    61–80 per cent

    81–100 per cent

    Jobling et al., 2006)

    3

  • 17�-estradiol (E2)

    Estrone (E1)

    OH

    OH

    O

    OH

    Ethynylestradiol EE2)

    OH

    OH

    ng/L 4

  • 0

    2

    4

    6

    8

    10

    12

    14

    16

    18

    England Japan

    N or

    m al

    is ed

    e xc

    re tio

    n E

    2 eq

    ui v

    ug /h

    ea d/

    d

    2% ( , 2001)

    %

    E2 (Johnson et al., 2006

    0.6 %

    ?

    ? ? ?

  • 6

    1) 2) 3)

    UK-J Phase 2 CORE 1 (2005 to 2009)

  • Estradiol (E2) Estriol (E3) Estrone(E1)

    ( or

    Ethynyl estradiol (EE2)

    O

    C ONa

    OH

    HO OH

    O OH

    SO

    O

    OH

    OH

    7

  • Sample

    SPE

    (GF/B)

    Oasis HLB

    STD

    NH2 Cartridge

    CH3CN/H2O (1:9),1ml

    MeOH 0.5% NH4OH/MeOH

    N2 Evap.

    LC-MS/MS LC-MS/MS

    Clean up

    Clean-up with NH2

    Change LC 50 mm 100 mm

    : 500 ml / : 1000 ml

    8

  • 0

    10

    20

    30

    40

    50

    60

    70

    80

    90

    100

    E1 E2 E3 EE2 E1-3S E2-3S E3-3S E1-3G E2-3G E3-3G

    C on

    ce nt

    ra tio

    n (n

    g/ L)

    Influent Effluent

    UK (n = 3)

    � E1, E2 EE2

    =Range

    9

  • � EE2 � E1 � E1

    n=29

    0

    10

    20

    30

    40

    50

    60

    70

    80

    90

    100

    E1 E2 E3 E1-3S E2-3S E3-3S E1-3G E2-3G

    C on

    ce nt

    ra tio

    n (n

    g/ L)

    0

    10

    20

    30

    40

    50

    60

    70

    80

    90

    100

    R em

    ov al

    e ffi

    ci en

    cy (

    % )

    Influent Effluent Removal

    10

  • 0

    20

    40

    60

    80

    100

    E1 E2 E3 EE2 E1-3S E2-3S E3-3S E1-3G E2-3G E3-3G

    R em

    ov al e ffi ci en

    cy ( %

    )

    Removal efficiency

    -80

    -60

    -40

    -20

    0

    20

    40

    60

    80

    100

    E1 E2 E3 EE2 E1-3S E2-3S E3-3S E1-3G E2-3G E3-3G

    R em

    ov al

    e ffi

    ci en

    cy (%

    ) E2,EE2

    E1

    Ja p

    an (

    n =

    9) U

    K (

    n =

    3)

    =Range

    11

  • 12

    � E1 EE2 E2

    � EE2

    0

    10

    20

    30

    40

    50

    C on

    ce nt

    ra tio

    n (n

    g/ L)

    Influent

    Effluent

    Downstream

    E1 E2 E3 EE2 E1-3S E2-3S E3-3S E2-3&17GE3-3&17G

    C o

    n ce

    nt ra

    tio n

    (n g

    /L )

  • 13

    Benson Sandhurst

    R. Thames Camberley

    Parking

    Sandhurst

  • 14

    E2 ?

    PST Reactor FST

    Qs

    Qs Qs

    PST Reactor FST

    Qs

    Qs

    kQs

    Qs

    kQs

    +

  • SRT

    15

    SRT=22 E1 E2

  • �� ..

    N=6

    0

    2

    4

    6

    8

    10

    12

    14

    16

    18

    20

    E1 E2 E3 E1-3S E2-3S E3-3S

    C on

    ce nt

    ra tio

    n (n

    g/ L)

    0

    20

    40

    60

    80

    100

    R em

    ov al

    e ffi

    ci en

    cy (

    % )

    Secondary Effluent After Ozonation Removal

    16

  • D7 D4

    D5

    D3

    D2

    D9

    D8 D6

    D1

    U2

    U1

    K1

    U9 U6

    U7

    U9 U10

    K7

    K9K8

    K6 K5

    K10

    K4 K2

    K3

    U3 U4

    U9

    17

    �E2 EE2 , E1 �E1 , �E1

  • (segment)

    (segment)

    STP

    STP

    STP

    60

    , , ,

    18

  • 0

    1

    2

    3

    4

    5

    6

    7

    8

    9

    10

    F ro

    m e

    D ar

    t

    T or

    rid ge

    C uc

    km er

    e

    G re

    at S

    to ur

    A ru

    n

    S oa

    r

    T em

    e

    C ro

    uc h

    C am

    W ea

    r

    T ee

    s

    E de

    n

    C al

    de r

    W yr

    e

    T af

    f

    T yw

    i

    E as

    te rn

    C le

    dd au

    C on

    w y

    M er

    se y

    Irw el

    l

    A ire

    D on

    B ly

    th

    E re

    w as

    h

    T re

    nt

    Id le

    Li ttl

    e O

    us e

    W av

    en ey

    T ha

    m es

    M ed

    w ay

    A vo

    n

    0

    1

    2

    3

    4

    5

    6

    7

    8

    9

    10 75% 25%

    19

    EEQ (ng/L) E

    st im

    at ed

    E E

    Q E

    2- eq

    u iv

    al en

    t, n

    g /L

    ng/L

  • Risk Class

    No risk

    At risk

    High risk

    LF2000-WQX

    (>1 ng/L EEQ)

    (>10 ng/L EEQ) 20

  • 0.0

    0.5

    1.0

    1.5

    2.0

    2.5

    3.0

    3.5

    4.0

    4.5

    5.0 R

    um oi

    S ar

    u

    T ok

    or o

    Is hi

    ka ri

    A ka

    Y on

    es iro

    K ita

    ka m

    i

    T su

    ru m

    i

    K uj

    i

    N ak

    a

    K ak

    eh as

    hi

    K ur

    ob e

    A ra

    S hi

    na no

    K ik

    u

    A be

    S yo

    un ai

    K is

    o

    K ita

    Y am

    at o

    Y ur

    a

    A sh

    id a

    A sa

    hi

    G ou

    no

    D ok

    i

    M on

    ob e

    N iy

    od o

    Y os

    hi no

    H om

    m yo

    K im

    ot su

    ki

    O oi

    ta

    C hi

    ku go

    0.0

    0.5

    1.0

    1.5

    2.0

    2.5

    3.0

    3.5

    4.0

    4.5

    5.0

    1ng/L

    30.6 18.0

    98Summer

    98Autumn

    99Spring

    99Summer

    99Autumn

    99Winter

    00Autumn

    01Autumn

    NA NA NA NA NA

    NA:No available data

    21

    E st

    ro g

    en ic

    A ct

    iv it

    ie s,

    E 2-

    eq u

    iv al

    en t,

    n g

    /L EEQ

    (ng/L)

    YES Assay w/ hER

  • 22

  • • • k=0.067 /h

    0

    1

    2

    3

    4

    0 1 2 3 4

    Estimated Concentration (ng/L)

    M ea

    su re

    d C

    on ce

    nt ra

    tio n

    (n g/

    L)

    Theoretical line (x=y)

    23

  • 24

  • � SRT E1 , O3 .

    25

    SRT + O3

    E1

    25

  • • EE2 E2

    • SRT

    Summary

    26

  • 27

    Acknowledgements

    12th Annual Scientific Workshop UK-Japan Co-operation for Research on Endocrine Disrupters in the Aquatic Environment held at Otterburn Hall Northumberland National Park UK on 2- 4 November 2010