CIRCULATING NUCLEIC ACIDSCELL-FREE NUCLEIC ACIDS

BARBORA VLKOVÁINSTITUTE OF MOLECULAR BIOMEDICINE

www.imbm.skbarbora.vlkova@imbm.sk

DNA is present in:

a) nucleus

b) mitochondria

c) cytoplasma

d) plasma

e) ...

X

X

Nucleus

Mitochondria

GA

ER

Nucleus

Mitochondria

GA

ER

Mitochondrial DNA

Nuclear DNA

Mandel P, Metais P. Les acides nucleiques du plasma sanguin chez l'homme. C R Acad Sci Paris 1948; 142:241-243.

Extracelulárna DNA

Lo YM et al.: Presence of fetal DNA in maternal plasma and serum. Lancet 1997; 350(9076):485-7.

Research of CNA

Oncology Fetal medicine

Traumatology Blunt trauma increases CNA (gDNA & mtDNA)

Burn injuries

Correlation with clinical status & prognosis Inflammation & sepsis & transplantation Diabetes mellitus ...

Marathon & CNA

AMI & CNA

Stroke & CNA

CNA in oncology

Detection of wild-type (WT) and mutant DNA in CSF, plasma, tumor, and blood cell

Genometastasis