Post on 19-May-2015
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Project: AFM testtowards investigation
of neurons andneuronal microtubules
Presented in PosLab (C368) on May 30th, 2007, at 15:15
by Mikael O. Bonnier
for the course Experimental Biophysics, TNF030with advisor Lars Henrik Dæhli Skjolding
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Chip with interdigitated electrodes, but these are pictures of thechip connections in the micrometer range in optical and AFMheight, respectively.
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Three contact mode cantilevers, a single intermittent contactmode cantilever, and three intermittent contact mode can-tilevers, respectively on one chip each.
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Adjusting laser beam and detector.
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QuitAFM image using worn tip. Note the missing data.
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The advanced optical microscope (Nikon TE2000-U) with theAFM mounted.
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Active vibration damper (Halcyonics Micro 40).
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Images of the marker pen cross in water using opticalmicroscope and AFM, respectively. Note the missing data.
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BioCellTM.
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Stem cells using x40 microscope lens.
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Invading bacteria using x10 microscope lens.
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More differentiated stem cells in optical directly overlayed withAFM height images (by Dr Rachel Owen at JPK Intruments
demonstrating the AFM in our lab).
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Conclusions
• Study the manuals and control theory
• Better if prism were made so that it could only fit one wayinto the AFM
• Many interesting experiments, that may require stem cells ge-netically modified so that they have different microtubule hairlengths, can be made using our new AFM
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Acknowledgements
• Lars Henrik Dæhli Skjolding
• Jan Tønnesen
• Jonas Tegenfeldt
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QuitHappy Summer!