Post on 31-Dec-2015
Spermatozoa heart (cryosection)
ELECTRON MICROSCOPY IMAGES OF VARIOUS MIT0CHONDRIA.
The heart cryosection has been reacted with an anti-cytochrome c oxidase antibody that is labeled with gold particles.
The internal structure of mitochondria [Review]Terrence G. Frey and Carmen A. Mannella Trends in Biochemical Sciences, 2000, 25:7:319-324
outer membrane
inner boundary membrane
cristae
crista junction
3D reconstructed electrontomogram of mitochondriaDr.Terry Frey; San Diego State University
2) Different states (fragmented, intermediate, reticulum)
A B Cfragmented intermediate reticular
10 m
- MitoTracker Red staining- osteosarcoma cells (143B)
-Osteosarcoma cells were arrested in G0 by FCS starvation for 60-72 h- Released by FCS addition- Stained with MitoTracker Red
Fragmented Reticular G0 G1 S G2/M
EM images of mitochondria from fibroblast cell lines. ABCD, different segments of an image of normal fibroblasts. C. Mitochondria from rh0 fibroblastcell.
A B
C D10 m 2.5 m
GF
P-E
1
Fre
e -
RF
P
Mer
ged
imag
e
24 hours post transfection
Distribution of Pyruvate dehydrogenase complex that has been tagged with GFP in fibroblast cells that show the mitochondrialr eticulum
Localization of GFP-tagged PDH after various times of incubation of cells. The complexes are essentially fixed with respect to the reticulum.
Ultra thin cryo section - bovine heartCORE II labeled with 10 nm gold particles Rob Gilkerson
Green: anti COX I antibodyRed: anti E2 antibody
Relative distribution of Cytochrome oxidase and PDH in a mitochondrial reticulum.