An animal cell

Spermatozoa heart (cryosection)

ELECTRON MICROSCOPY IMAGES OF VARIOUS MIT0CHONDRIA.

The heart cryosection has been reacted with an anti-cytochrome c oxidase antibody that is labeled with gold particles.

The internal structure of mitochondria [Review]Terrence G. Frey and Carmen A. Mannella Trends in Biochemical Sciences, 2000, 25:7:319-324

outer membrane

inner boundary membrane

cristae

crista junction

3D reconstructed electrontomogram of mitochondriaDr.Terry Frey; San Diego State University

Thin section of heart tissue showing the reticulum structure of mitochondria visualized by EM.

3) 3D view of reticulum

143B osteosarcomaGFP-pH transfectedConfocal microscope3D projection

2) Different states (fragmented, intermediate, reticulum)

A B Cfragmented intermediate reticular

10 m

- MitoTracker Red staining- osteosarcoma cells (143B)

-Osteosarcoma cells were arrested in G0 by FCS starvation for 60-72 h- Released by FCS addition- Stained with MitoTracker Red

Fragmented Reticular G0 G1 S G2/M

glucose

galactose

glucose

galactose

hela

143B

EM images of mitochondria from fibroblast cell lines. ABCD, different segments of an image of normal fibroblasts. C. Mitochondria from rh0 fibroblastcell.

A B

C D10 m 2.5 m

GF

P-E

1

Fre

e -

RF

P

Mer

ged

imag

e

24 hours post transfection

Distribution of Pyruvate dehydrogenase complex that has been tagged with GFP in fibroblast cells that show the mitochondrialr eticulum

Localization of GFP-tagged PDH after various times of incubation of cells. The complexes are essentially fixed with respect to the reticulum.

Ultra thin cryo section - bovine heartCORE II labeled with 10 nm gold particles Rob Gilkerson

Green: anti COX I antibodyRed: anti E2 antibody

Relative distribution of Cytochrome oxidase and PDH in a mitochondrial reticulum.

MICOS complex isolated from human heart by antibody immunocapture