3' Race
Cell 101001
Realtime
Tpa 2013
A Practical Guide to Cellular and Molecular Research Methods in Immunology
End Repair of double-stranded cDNA Adenylation (A-Tailing) A A PCR Amplification using a Universal primer and index primer A U T U T A T U Barcode.
Fragmenting genomic DNA for cloning –Random methods are best Mechanical shearing: sonication, nebulizer Nuclease treatment (usually restriction digest):
Recombinant DNA Technology (Part II). Genomic VS cDNA Library Size of the DNA fragments can be prepared by different type of restriction enzymes. However,
Experimenttal part
