Biotechnolgy. Basic Molecular Biology Core of biotechnology.
Chapter 3 Recombinant DNA Technology (genetic engineering)
PCR Techniques. Basics of PCR Primers –15-60bp (60bp is limit synthesized by IDT) –Annealing temp ideally >55C (portion that anneals to your template)
MOLECULAR BIOLOGY – Molecular biology techniques MOLECULAR BIOLOGY TECHNIQUES I. DNA isolation and fragmentation Recombinant DNA Gel electrophoresis Hybridization.
Chapter 10 Genetic Engineering: A Revolution in Molecular Biology.
Troubleshooting DNA Sequences: Guidelines and Suggestions.
Plasmids Isolation of Plasmid DNA from Bacteria Properties of a Cloning/Expression Vector (Plasmid) Uses of Expression Vectors.
The genome of prokaryotes and eukaryotes- nuclear and extranuclear genetic organization.
Lecture 9: 1 10/11/2006 DNA structure: Non-helical secondary structures: non-helical, palindrome, hairpin, and cruciform stabilized by negative DNA supercoiling.
Objectives To understand the structure of bacteria –Compared to viruses Understand Eubacteria vs. Archaebacteria How bacteria classified –Kingdom, phyla,
Chapter 20 (Schaechter’s). SBM 2044 Lecture 7 Tetanus and Botulinum Neurotoxins Clostridium tetani Clostridium botulinum Similar neurotoxins – different.
Bacteriology Stack Slackers Facts by Mike Ori. Disclaimer The information represents my understanding only so errors and omissions are probably rampant.