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DNA Recombinant Technology What and Why? What?: A gene of interest is inserted into another organism, enabling it to be cloned, and thus studied more effectively Why?: Detailed…
1. Recombinant DNARecombinant DNA TechnologyTechnology Tapeshwar Yadav (Lecturer) BMLT, DNHE, M.Sc. Medical Biochemistry 2. A – A good student is liked by teacher G –…
Recombinant DNA Technology Restriction endonucleases - Blunt ends and Sticky ends Restriction endonuclease and DNA ligase yield Recombinant DNA DNA cloning Polylinker â…
Recombinant DNA Technology Recombinant DNA Technology A gene must be isolated and well characterized before it can be used in genetic manipulations. One method of isolating…
Recombinant DNA Technology Deqiao Sheng PhD Dept. Of Biochemistry and Molecular Biology Reference Books Hortons’ Principles of Biochemistry 4th edition Molecular Biology,…
Slide 1 Recombinant DNA technology DNA isolated from two sources and cut by the same enzyme Gene of interest Recombinant DNA Plasmid (vector) Transgenic organisms Useful…
Recombinant DNA Technologyâ¦â¦â¦.. BTEC3301 Polymerase Chain Reaction (PCR) Introduction The Polymerase Chain Reaction (PCR) provides an extremely sensitive means of…
Recombinant DNA Technology Dr. Hui LI Office : S408 Tel: 26538722 [email protected] Isolation of target gene Selection and construction of vectors Ligation of target DNA…
DNA Technology & Genomics Chpt. 20 The use of recombinant DNA technology has already impacted your life in ways that you might not expect. How has recombinant DNA affected…
Genetics & DNA Technology Genetics & DNA Technology 65. Gregor Mendel crossed a true-breeding tall plant (TT) with a true-breeding short plant (tt). What are the…
Recombinant DNA Technology Methods for Isolating, Amplifying and Studying Specific DNA Sequences Cutting and Joining DNA Fragments Restriction Enzyme: cleaves DNA at specific…
Recombinant DNA Technology Dr. Hui LI Office : S408 Tel: 26538722 Topic 3 Transfer of target gene into receptor cell Bacteria that are capable of uptaking the foreign DNA…
Recombinant DNA Technology Dr. Hui LI Office : S408 Tel: 26538722 Isolation of target gene Selection and construction of vectors Ligation of target DNA and vector Transformation…
Recombinant DNA Technology Needles in Haystacks How to find one gene in large genome? A gene might be 1/1,000,000 of the genome. Three basic approaches: 1. Polymerase chain…
Recombinant DNA Technology Recombinant DNA Technology Review What are enzymes? What function do they serve? What makes up the DNA sequence? How is the genetic code determined…
RECOMBINANT DNA TECHNOLOGY PART 2 Topics Genomic libraries Id a specific clone or sequence within a library Transgenic plants Transgenic animals Timely topics Genomic library…
RECOMBINANT DNA TECHNOLOGY DNA fragments from different sources can be joined together MEDICAL APPLICATIONS The discovery of the restriction-modification system of bacteria…
Recombinant DNA Technology Dr. Hui LI Office : S408 Tel: 26538722 Topic 5 Expression of recombinant gene (Prokaryotes) 遗传信息从DNA到蛋白质的传递过程——中心法则（central…
PowerPoint Presentation RECOMBINANT DNA TECHNOLOGY Presenter : saranya . S Moderator : dr.v.balasubramaniyan 1 1 DNA CLONING DNA cloning protocol Plasmid cloning Bacteriophage…
Recombinant DNA technology M.Prasad Naidu MSc Medical Biochemistry, Ph.D,. Introduction:Based on the concept of gene recombinations. Definition:Encompasses a number of experimental…