Collaborative E-Learning application for
electroporation-based biomedical applications
June 12th-14th, 2013
Selma CorovicSelma Corovic
Background and motivation
- Electroporation of cells and tissues – Electroporation of cells and tissues – human, animal or human, animal or plant cells for different biomedical plant cells for different biomedical andand biotechnological biotechnological applicationsapplications
- Electroporation based biomedical applications Electroporation based biomedical applications require multidisciplinary expertise: oncologists, require multidisciplinary expertise: oncologists, physicists, engineers…physicists, engineers…
Collaborative E-Learning application
clinical electrochemotherapyclinical electrochemotherapy - cancer - cancer treatment treatment
Oncology Oncology Biomedical engineering Biomedical engineering
E-Learning enviromentE-Learning enviroment(EBTT)(EBTT)
E-Learning enviromentE-Learning enviroment(EBTT)(EBTT)
E-learning application on cells and tissues
E-learning application on cells and tissues
ResearchResearch
Clinical trialsClinical trials
Improving the contentImproving the contentbased on resultsbased on results
ColColllecting ecting the knowledgethe knowledge
Designing Designing the contentthe content
E-Learning application
++
EvaluationEvaluation (pedagogical efficiency and usability) (pedagogical efficiency and usability)
E-Learning enviromentE-Learning enviromentE-Learning enviromentE-Learning enviroment
Electroporation based technologies and treatmentsElectroporation based technologies and treatmentsInternational SCIENTIFIC WORKSHOP and POSTGRADUATE COURSEInternational SCIENTIFIC WORKSHOP and POSTGRADUATE COURSE
November 18-24, 2012November 18-24, 2012, Ljubljana, Slovenia, Ljubljana, Slovenia
LecturesLectures Practical workPractical work
Lectures were recordedLectures were recorded
molecules cell membrane
ElectroporationElectroporation
Important parameter for effective electroporationImportant parameter for effective electroporation local electric field distribution E [V/cm]!local electric field distribution E [V/cm]!
Cell membrane Cell Tissue
Electroporated
Local electric field distribution E >Erev
(a)The electroporation of cell membrane first occurs within the cell area facing the electrodes
(b)Different electroporation regimes: reversible Erev < E < Eirrev and irreversible E >Eirrev
UTI = −1.5 r E cos (φ)
Electroporation is used in: - clinical electrochemotherapyclinical electrochemotherapy, non-viral gene electrotransfer for gene therapy and DNA vaccination, transdermal drug delivery…- liquid food sterilization, water treatment, electrofusion of cells…
Applications of Electroporation
biomedical
timeElectrodes
Electric pulse generator
Tumour
Chemotherapeutic surrounds the cells
Increased membrane permeability allows access to the cytosol
Pores reseal
Injecting chemotherapeutic
Electric pulse application
Electrochemotherapy =
= chemotherapy + electroporation
Local electric field distribution:Local electric field distribution:
drug injection
++
U/dU/d
ResultResult
Protocols used in clinical electrochemotherapy today include:
8 pulses of 100 µs duration, f=1 Hz or f=5 KHz, U/d =1300 V/cm
Protocols and local electric field distribution in tumorsProtocols and local electric field distribution in tumors
the entire the entire volume of the
volume of the tumortumorneeds to be
needs to be subjected to
subjected to the E ≥ Erevthe E ≥ Erev
Importance of an adequate local electric field for successful tissue electroporation (example: unsuccessful tumor treatment)
the entire the entire volume of the
volume of the tumortumorneeds to be
needs to be subjected to
subjected to the E ≥ Erevthe E ≥ Erev
Clinical use of electrochemotherapy
Reimbursed in:Germany, UK, Italy, Portugal, Denmark, Greece, Slovenia.
Poland – in progress for reimbursement. ECT approved by Minister of Health.
Number of patients
No. of clinical centres
2006 130 SOP
2007 300 37
2008 600 53
2009 800 55
2010 1000 83
2011 1500 92
2012 3000 108
Clinical use of electrochemotherapy
E-Learning enviromentE-Learning enviroment(EBTT)(EBTT)
E-Learning enviromentE-Learning enviroment(EBTT)(EBTT)
E-learning application on cells and tissues
E-learning application on cells and tissues
ResearchResearch
Clinical trialsClinical trials
Improving the contentImproving the contentbased on resultsbased on results
ColColllecting ecting the knowledgethe knowledge
Designing Designing the contentthe content
E-Learning application
++
EvaluationEvaluation (pedagogical efficiency and usability) (pedagogical efficiency and usability)
Laboratory of bio cybernetics
Lea Retelj, Ajda Bicek, Matevz Leskovsek, Jasna Krmelj, Tadej Kotnik, Dusa Hodzic, Marko Usaj, Gorazd Pucihar, Alenka Macek Lebar, Samo Mahnic, Barbara Mali, Tomaz Jarm, Jaka Cemazar, Selma Corovic, Denis Pavliha, Bor Kos, Masa Kanduser, Natasa Pavselj, Janez Zigon, Ivan Pavlovic, Stanislav Rebersek,
David Slavic, Melvin Essone, Jure Jelenc, Matej Rebersek, Tina Batista Napotnik, Mitja Gliha, Sasa Haberl, Peter Kramar, Igor Marjanovic, Barbara Zorec, Matej Kranjc, Damijan Miklavcic.
(Missing from the photo: Karel Flisar, Marija Marcan, Andraz Polak)
http://lbk.fe.uni-lj.si
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