Titration of Abundance for Purified GST-Nups

A GST-Nsp1h (1-172) B GST-Nup116h (165-715) C GST-Nup100h (1-640)

D GST-Nup145h (1-242) E GST-Nup1h (798-1076) F GST-Nsp1 (377-471) K>R

G GST-Nsp1 (377-471) wild type H GST-Nup42 (1-382)

Titration of Abundance for Purified GST-Nups

Group GST-Nup Amino Acids Molecular Weight (daltons)

A GST-Nsp1h (1-172) 1-172 46,653

B GST-Nup116h (165-715) 165-715 83,844

C GST-Nup100h (1-640) 1-640 93,835

D GST-Nup145h (1-242) 1-242 53,498

E GST-Nup1h (798-1076) 798-1076 57,092

F GST-Nsp1 (377-471) K>R 371-471 39,841

G GST-Nsp1 (377-471) wild type 377-471 39,365

H GST-Nup42 (1-382) 1-382 67,144