Abstracts / Journal of Biotechnology 185S (2014) S37–S125 S61

Analyses of enzymes involved in thedegradation of catechol and o-cresol byAspergillus fumigatus strain, isolated fromAntarctic soil

Maria Gerginova, Katya Litova, Jordan Manasiev,Nadejda Peneva, Zlatka Alexieva ∗

Institute of Microbiology, Bulgarian Academy ofSciences, Sofia, Bulgaria

E-mail address: zlatkama@yahoo.com (Z. Alexieva).

The examined strain of Aspergillus fumigatus AL8 was isolated fromsoil probes taken in the region of Antarctica. This study showedthe different efficiency of the investigated strain with respect tothe degradation of phenol derivatives, as catechol (hydroxylatedphenol) and o-cresol (methylated phenol). The strain was able toutilize both examined compounds. The complete utilization of cat-echol as a sole carbon source was performed for about 24 h whilethe o-cresol was degraded and utilized much slower (48 h). Thestrain was cultivated in a medium containing 0.3 g/l catechol or0.3 g/l o-cresol as single carbon substrates. The intracellular activ-ities of the both key enzymes, phenol hydroxylase and catechol1,2-dioxygenase in cells of A. fumigatus AL8 were determined.The catechol 1,2-dioxygenase showed the highest enzyme activ-ity (0.332 U/mg protein) during the logarithmic growth phase ina medium with catechol included. The activity of this enzyme incells, cultivated with o-cresol was much lower - 0.053 U/mg pro-tein. The enzyme phenol hydroxylase kept relatively low level ofactivity in both studied degradation processes. These results aredirectly linked to the different inhibitory effect exerted by differ-ent substitution groups in the structure of the utilized compoundson the strain’s cells.

http://dx.doi.org/10.1016/j.jbiotec.2014.07.205

The influence of plant colonization by bacterialstrains isolated from plants with oilcontaminated soil on plant growth

Anel Omirbekova 1,∗, Togzhan Mukasheva 1,Ramza Berzhanova 1, Gauhar Demeubaeva 2,Sandugash Ibragimova 2

1 Al-Farabi Kazakh National University Kazakhstan,Kazakhstan2 Scientific Research Institute of Ecological Problems,Kazakhstan

E-mail address: anel.83@mail.ru (A. Omirbekova).

Among the many pollutants of the environment, crude oil is acommon contaminant of water and soil. Phytoremediation of con-taminated soils involves the use of plants and their associatedmicroorganisms. Inoculation of plants with high bacteria havingthe degradation abilities is one of the possible ways to improvethe effectiveness of bioremediation. The aim of work was to eval-uate the growth characteristics of plants after inoculation withbacterial strains – destructors of oil hydrocarbon. For inoculation,the hydrocarbon degrading microorganisms isolated from rhizo-sphere and rhizoplane of plants were used. Gnotobiotic systemsincluded sterile sand, plants, oil and bacterial strains of Pseu-domonas, Rhodococcus genera. One of the evaluated parameters wasthe toxic effects of oil on plants, as well as the protective effect onplants of each of the microorganisms. It has been found that theoil has a significant phytotoxic effect which was expressed in sup-pressing the growth of shoots and roots. The measurement of thelength of shoots and roots showed that the introduction of microor-

ganisms increases the growth in two times than in the controlsamples without inoculation of plants with bacterial strains. Thebest protective effect was observed in model system containingmicroorganism strain of Rhodococcus genera.

http://dx.doi.org/10.1016/j.jbiotec.2014.07.206

Creation of consortium based on cyanobacteriafor agrobiotechnological use

Gul Baimakhanova 1,∗, Bolathan Zayadan 1, GulnarOraz 1, Amankeldy Sadanov 2, KenzhegulBolathan 1

1 Al-Farabi Kazakh National University, Kazakhstan2 Institute of Microbiology and Virology, Almaty,Kazakhstan

E-mail address: bgulb@mail.ru (G. Baimakhanova).

Cyanobacteria play an important role in soil fertility increas-ing by atmospheric nitrogen fixing. Prospects of nitrogen-fixingcyanobacteria application in agriculture is related to their par-ticipation in the nitrogen cycle which significantly affects theproductivity of higher plants. The aim of work is creating ofconsortium based on cyanobacteria, microalgae and azotobacter.For consortium creation, Anabaena variabilis and Nostoc salsicolacyanobacteria isolated from Kazakhstan rice fields were selected.The optimal culture conditions of cyanobacteria were selected. Toenhance the process of nitrogen fixation in the consortium, Azo-tobacter sp. strain was added. Chlorella vulgaris was added duringcultivation of consortium to neutralize pH of the medium. Theeffect of consortium on seed germination and seedling growth ofrice was tested. Results of experiments showed that treatment ofrice seeds by suspension of A. variabilis, Chlorella vulgaris, Azoto-bacter sp. consortium has little stimulating effect on germination,stem length and plant development. On the basis of isolated ricepaddies cyanobacteria two microbial consortium ZOB-1 (A. vari-abilis – Chlorella vulgaris – Azotobacter sp.) and ZBOB-2 (N. calsicola– Chlorella vulgaris – Azotobacter sp.) were created. That maybe proposed for rice crops agricultural use in biotechnology forimprovement of the germination of plants and soil enrichment bynitrogen.

http://dx.doi.org/10.1016/j.jbiotec.2014.07.207

Removal of ethidium bromide from aqueoussolution by using Photomogetom crispus

Mahir Demir ∗, Osman Gulnaz

Department of Biotechnology, Institute of NaturalApplied Sciences University of Cukurova, 01330Adana, Turkey

E-mail address: mahirdmr@hotmail.com (M. Demir).

Adsorption is a effective technology to remove dyes from aque-ous solutions. In this study removal of fluorescent dye ethidiumbromide by dried Photomogetom crispus was investigated in batchsystems. Effect of pH and initial dye concentrations on adsorp-tion were determined. The suitable pH was determined as 3.0 and20 ◦C. The theoretical max biosorption capacity of Photomogetomcrispus was 0.21 mM g−1 at 20 ◦C for 0.06 mm particle size. Theequilibrium data fitted very well to both Langmuir isotherm mod-els. The pseudo-first and second-order kinetic models were used todescribe the kinetic data. The experimental data fitted to second-order kinetic model. Increasing of the initial dye concentrationsincreased the adsorption of ethidium bromide. FT-IR results shows