Solutions for
Cancer Metabolism Research
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Assay Kits, Metabolites, and Reagents Specific to Your Pathway of Interest1. Aerobic Glycolysis
2. Fatty Acid Metabolism
3. Mevalonate Pathway
4. Glutamine Metabolism
and More
sigma.com/cancermetabolism
2 Solutions for Cancer Metabolism Research
Advantages:• Rapid and high throughput ready
• Reproducible and supported by industry-leading technical service
• Contain all reagents, probes, and standards required for assay
• Colorimetric and fluorometric formats
• Economical pricing
• Offered with associated metabolites and reagents
METABOLISM ASSAY KITS FROM SIGMA-ALDRICH®
Convenient results you can trust.
For further info visit sigma-aldrich.com/cancermetabolism
3For more information, visit sigma-aldrich.com
Cat. No. NameL2625 l-Lactic Dehydrogenase from bovine heart Type III
L1378 l-Lactic Dehydrogenase from bovine muscle Type X
Cat. No. NameG9545 Anti-GAPDH antibody produced in rabbit, ~1 mg/ml, affinity
isolated antibody, buffered aqueous solution
SAB1100050 Anti-LDHA (216-228) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
SAB4200095 Anti-PKM2 (isoform M2) antibody produced in rabbit, ~1.5 mg/ml, affinity isolated antibody
Cat. No. NameGAGO20 Glucose (GO) Assay Kit, sufficient for 20 tests
GAHK20 Glucose (HK) Assay Kit, sufficient for 20 tests
MAK064 Lactate Assay Kit, sufficient for 100 colorimetric or fluorometric tests
MAK066 Lactate Dehydrogenase Activity Assay Kit, sufficient for 500 colorimetric tests
MAK065 Lactate Assay Kit II, sufficient for 100 colorimetric tests
MAK037 NAD/NADH Quantitation Kit, sufficient for 100 colorimetric tests
MAK039 Acetyl-Coenzyme A Assay Kit, sufficient for 100 fluorometric tests
MAK083 Glucose Uptake Colorimetric Assay Kit, sufficient for 100 colorimetric tests
MAK091 Hexokinase Colorimetric Assay Kit, sufficient for 100 colorimetric tests
MAK071 Pyruvate Assay Kit, sufficient for 100 colorimetric or fluorometric tests
1. AEROBIC GLYCOLYSIS AND THE WARBURG EFFECT
Investigation of the metabolic basis of oncogenesis continues with fervor in the field of cancer research. Although the Warburg effect was discovered in 1924, aerobic glycolysis remains a central pathway of interest in cancer metabolism studies. 1,2
1. Boroughs, L. K. et al. Metabolic Pathways Promoting Cancer Cell Survival and Growth. Nature Cell Bio. 17, 351-359 (2015).
2. Seyfried, T. N. et al. Cancer as a Metaoblic Disease: Implications for Novel Therapeutics. Carcinogenesis, 35, 515-527 (2014)
Metabolites, Inhibitors, and Activators of Aerobic Glycolysis
Proteins Involved in Aerobic Glycolysis
Antibodies Against Proteins Involved in Aerobic Glycolysis
Cat. No. Name DescriptionG7528 d-(+)-Glucose BioXtra, ≥99.5% (GC) Metabolite
L7022 Sodium l-lactate ~98% Metabolite
P5280 Sodium pyruvate powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99%
Metabolite
G7879 d-Glucose 6-phosphate sodium salt Sigma Grade, crystalline
Metabolite
A9978 AICAR ≥98% (HPLC), powder Inhibitors and Activators of the AMPK pathway
D150959 1,1-Dimethylbiguanide hydrochloride 97%
Inhibitors and Activators of the AMPK pathway
P5499 Dorsomorphin ≥98% (HPLC) Inhibitors and Activators of the AMPK pathway
D9533 Deferoxamine mesylate salt powder, ≥92.5% (TLC)
HIF Prolyl-Hydroxylase Inhibitor
D3695 DMOG ≥98% (HPLC) HIF Prolyl-Hydroxylase Inhibitor
D8375 2-Deoxy-d-glucose ≥98% (GC), crystalline
Hexokinase Inhibitor
View our complete cancer metabolism research portfolio at sigma.com/cancermetabolism
Assay Kits for Studying Aerobic Glycolysis Metabolites
4 Solutions for Cancer Metabolism Research
2. FATTY ACID METABOLISM IN CANCEROncogenic progression is often associated with the increased activity and expression of enzymes involved in fatty acid synthesis, such as fatty acid synthase (FASN), acetyl-CoA carboxylase (ACC), and ATP citrate lyase (ACL).
Assay Kits for Studying Fatty Acid Metabolism
Inhibitors of Fatty Acid Biosynthesis Pathways
Metabolites of Fatty Acid Biosynthesis Pathways
Cat. No. NameMAK044 Free Fatty Acid Quantitation Kit, sufficient for 100 colorimetric or
fluorometric tests
MAK039 Acetyl-Coenzyme A Assay Kit, sufficient for 100 fluorometric tests
MAK085 Lipid Peroxidation (MDA) Assay Kit, sufficient for 100 colorimetric or fluorometric tests
MAK086 Acetate Colorimetric Assay Kit, sufficient for 100 colorimetric tests
MAK041 β-Hydroxybutyrate Assay Kit, sufficient for 100 colorimetric tests
Cat. No. NameE4143 (−)-Epigallocatechin gallate ≥95%
Q4951 Quercetin ≥95% (HPLC), solid
C2389 Cerulenin ≥98% (HPLC), from Cephalosporium caerulens
A3145 Apigenin ≥97% (TLC), from parsley, powder
Cat. No. NameA2056 Acetyl coenzyme A sodium salt ≥93% (HPLC), powder
N7505 β-Nicotinamide adenine dinucleotide 2'-phosphate reduced tetrasodium salt hydrate ≥97% (HPLC)
O4126 Oxaloacetic acid ≥97%
P9767 Sodium palmitate ≥98.5%
F1506 Sodium fumarate dibasic ≥99%
M4263 Malonyl coenzyme A lithium salt ≥90% (HPLC)
View our complete cancer metabolism research portfolio at sigma.com/cancermetabolism
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Proteins Involved in the Mevalonate PathwayCat. No. NameH7039 3-Hydroxy-3-methylglutaryl-CoA reductase human solution
M6908 Myeloperoxidase from human leukocytes
View our complete cancer metabolism research portfolio at sigma.com/cancermetabolism
Assay Kits for Studying the Mevalonate Pathway
Metabolites, Inhibitors, and Activators of the Mevalonate Pathway
Cat. No. NameMAK043 Cholesterol Quantitation Kit, sufficient for 100 colorimetric or
fluorometric tests
CS1090 HMG-CoA Reductase Assay Kit, sufficient for 30 assays
MAK069 MPO Fluorometric Activity Assay Kit, sufficient for 100 fluorometric tests
Cat. No. Name DescriptionG6025 Geranylgeranyl pyrophosphate
ammonium salt solution, ≥95% (TLC), ~1 mg/mL in methanol: NH4OH (7:3)
Metabolite
F6892 Farnesyl pyrophosphate ammonium salt methanol:ammonia solution, ≥95% (TLC)
Metabolite
S6196 Simvastatin ≥97% (HPLC), solid Inhibitor of HMG-CoA Reductase
M2537 Mevastatin ≥95% (HPLC), powder Inhibitor of HMG-CoA Reductase
SML0223 Zoledronic acid monohydrate ≥98% (HPLC)
Inhibitor of Farnesyl diphosphate synthase
3. MEVALONATE PATHWAY IN CANCERThe mevalonate pathway, associated with a diversity of cellular functions, also continues to be vigorously studied for its role in various types of cancer. 1,2
1. Jiang, P. In vitro and In Vivo Anticancer Effects of Mevalonate Pathway Modulation on Human Cancer Cells. Br. J. Cancer, 111, 1562-1571 (2014).
2. Yeganeh, B. Targeting the Mevalonate Cascade as a New Therapeutic Approach in Heart Disease, Cancer and Pulmonary Disease. Pharmacol. Ther. 143, 87-110 (2014).
NAVIGATE TOWARD METABOLOMIC DISCOVERY.Target your metabolism research with Sigma® Life Science’s comprehensive metabolomic resources.
Bionavigate.Sigma Life Science metabolites, enzymes, separation tools and technologies help you navigate the metabolic pathways to biomarker discovery. Our comprehensive offering of unique isolated and synthetic metabolites includes the amino acid, carbohydrate, lipid and TCA Cycle metabolite libraries.
Now featuring tandem mass spectrometry data from The Scripps Center for Metabolomics METLIN Metabolite Database for several metabolites.
Find metabolites for your research needs sigma.com/metabolomics
6 Solutions for Cancer Metabolism Research
Assay Kits for Studying Glutamine Metabolism
Metabolites, Inhibitors, and Activators of Glutamine Metabolism
Cat. No. NameMAK037 NAD/NADH Quantitation Kit, sufficient for 100 colorimetric tests
AA0100 Ammonia Assay Kit
MAK039 Acetyl-Coenzyme A Assay Kit, sufficient for 100 fluorometric tests
MAK004 Glutamate Assay Kit, sufficient for 100 colorimetric or fluorometric assays
GLN1 Glutamine and Glutamate Determination Kit
Cat. No. Name DescriptionG7513 L-Glutamine solution 200 mM,
solution, sterile-filtered, BioXtra, suitable for cell culture
Metabolite
A2056 Acetyl coenzyme A sodium salt ≥93% (HPLC), powder
Metabolite
G8415 L-Glutamic acid from non-animal source, meets EP testing specifications, suitable for cell culture, 98.5-100.5%
Metabolite
D2141 6-Diazo-5-oxo-L-norleucine crystalline
Inhibitor of various glutaminases
C13408 O-(Carboxymethyl)hydroxylamine hemihydrochloride 98%
Inhibitor of Aminobutyrate aminotransferase
View our complete cancer metabolism research portfolio at sigma.com/cancermetabolism
4. GLUTAMINE METABOLISM IN CANCERMany of the signaling pathways that promote oncogenesis also reprogram glutamine metabolism. In particular, cancer cells commonly rely on glutamate anaplerosis to refill the TCA cycle.
New Interactive Metabolomic Pathway ToolNow your metabolites, enzymes, and selected pathways are searchable and interactive!
sigma.com/pathways
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