1. Recombinational variability for combining ability among F4
barbadense lines, molecular marker and genetic transformation study
in cotton Chairperson Dr .S.S.PATIL Yanal Alkuddsi PGS 09AGR 4949
Department of Genetics & plant Breeding
2. Contents Introduction Objectives Review of Literature
Material and Methods Results and Discussion
3. Cotton improvement programmes primarily lay emphasis on
development of hybrids, which have contributed in improving the
productivity of cotton. Many population improvement schemes are
followed in cross pollinated crops to increase genetic diversity,
to create heterotic groups and exploit them. There is a steep rise
in production of long staple cotton, while production of extra long
staple, short staple and even medium staple cotton has come down
drastically. The success of Interspecific hybrids has lead to
overcome the acute shortage of ELS cotton which the country was
experiencing during 1970s. Realizing the need for developing
potential interspecific (HB) hybrids, a details study was initiated
at UAS Dharwad during 2007/08 to identify Hirsutum and Barbadense
genotypes capable of giving potential interspecific hybrids.
Molecular markers increasingly detect locus differences among
genotypes and represent a powerful tool for the assessement of
genetic diversity in plant species Interspecific hybrids are known
to be more susceptible to biotic stress. It is hence important to
develop Bt version for interspecific hybrid.
4. 0 50 100 150 200 250 300 350 400 03-04. 04-05. 05-06. 06-07.
07-08. 08-09. 09-10. 10-11. 11-12. Quantityinlakhbalesof170kgs Year
Staple-wise production of cotton in the Bt Cotton Era Short (Below
20.0 mm) Medium/Medium Long (20.5 to 27 mm) Long Staple (27.5 to
32.0 mm) Extra Long staple (32.5 mm & above) TOTAL The lopsided
Change in Composition of Fibre classes in Cotton At the time of
Independence India was mostly producing Short staple cotton
Hirsutum Cottons brought boost in Medium staple cotton production
Interspecific Hybrids brought self sufficiency and even export of
ELS cotton With the onset of Bt cotton era, there is an Increase in
total cotton production and Long Staple cotton production but a
relative reduction in Other classes Declaresing Diversity : from
Fibre quality angle What is the impact of this?
5. Development of Hirsutum vs Barbadense heterotic groups.
Exploiting heterotic groups by creation of recombinational
variability in G.barbadense F4 population for ability to combine
with selected diverse G. hirsutum testers. To determine combining
ability effects (gca and sca), variances (GCA and SCA), combining
ability patterns of these Barbadense lines. Identifying suitable
Barbadense lines for developing heterotic sub populations against
Hirsutum testers. Determining correlations, direct and indirect
effects of component characters on seed cotton yield. Evaluation of
genetic diversity in G. barbadense cotton lines and G. hirsutum
testers based on SSR molecular markers. Relationship between
molecular diversity and hybrid performance in interspecific hybrids
of cotton. Standarizing `in planta Agrobacterium tumefacience
mediated genetic transformation protocol to develop new events by
transforming G. hirsutum based on Cry1Ac-Cry1Ec genes .
6. 1. Estimation of heterosis 2. Combining ability effects and
variance 3. Development of heterotic groups and exploiting them 4.
Studies of correlation and path coefficient analysis 5. Molecular
marker study in cotton 6. In planta genetic transformation
7. 1. Development of heterotic groups 1.1 Line x Tester for
confirmation of interspecific heterotic groups In this experiment,
92 (YHB trial) interspecific hybrids (G.hirsutum x G.barbadense)
along with three checks (RAHB 87, MRC 6918 and DCH 32) subjected to
Line x Tester analysis (23 hirsutum testers and 4 barbadense
lines), was laid out in Randomized Block Design (RBD) with two
replications. Each entry was sown in 2 row plots spaced at 90 cm
with recommended dose of fertilizer and seeds were sown on
28-6-2010, 2-3 seeds were dibbled per spot in each row and thinning
was attended to retain one healthy plant per hill at 25 days after
sowing. All the recommended package of practices were followed to
rise healthy crop.
8. 2.1 Mean per se performance of best HB interspecific hybrids
For mean performance of 49 (best HB trial) hybrids, the hybrids
along with three checks (RAHB 87, MRC 6918 and DCH 32) was laid out
in Randomized Block Design with two replications. Each hybrid was
sown in 4 rows plots with spacing of 90 cm x 60 cm with recommended
dose of fertilizer and the trial was sown on 27/6/2010 .
9. 2. Evaluation of recombinational variability for combining
ability 2.1 Choice of the material Fig 1. Schematic representation
of research activities
10. Sl. No. Cotton / Entry 2.5% SL (mm) % Micronaire value
Maturity Ratio Tenacity (g/t) Elongation % 1 (DB 533 x DB 534 F3
IPS 62) 33.53 44.80 3.11 0.62 30.03 6.03 2 (DB 533 x DB 534 F3 IPS
49) 33.5 45 3.1 0.62 30.0 6.0 3 (DB 533 x DB 534 F3 IPS 23) 35.7 47
3.2 0.63 29.8 5.6 4 (DB 533 x DB 534 F3 IPS 36) 35.8 48 3.5 0.67
29.4 5.6 5 (DB 533 x DB 534 F3 IPS 15) 36.7 47 3.0 0.59 29.3 5.5 6
(DB 533 x DB 534 F3 IPS 1) 37.7 46 3.0 0.59 28.5 5.8 7 (DB 533 x DB
534 F3 IPS 33) 37.4 47 3.2 0.63 28.4 5.4 8 (DB 533 x DB 534 F3 IPS
24) 36.8 46 3.2 0.62 28.3 5.6 9 (DB 533 x DB 534 F3 IPS 16) 35.4 48
3.3 0.63 28.3 5.6 10 (DB 533 x DB 534 F3 IPS 52) 33.2 47 3.9 0.69
28.3 6.1 11 (DB 533 x DB 534 F3 IPS 12) 37.1 46 3.2 0.63 28.3 5.3
12 (DB 534 x DB 533 F3 IPS 22) 32.2 47 3.2 0.65 28.2 6.0 13 (DB 533
x DB 534 F3 IPS 14) 36.9 48 3.4 0.65 28.2 5.4 14 (DB 533 x DB 534
F3 IPS 34) 38.2 46 3.2 0.62 28.2 5.5 15 (DB 533 x DB 534 F3 IPS 55)
35.7 46 3.5 0.65 28.0 5.4 16 (DB 533 x DB 534 F3 IPS 17) 32.1 46
3.0 0.64 27.8 6.1 17 (DB 533 x DB 534 F3 IPS 32) 38.4 45 3.1 0.62
27.8 5.4 18 (DB 533 x DB 534 F3 IPS 38) 38.4 47 3.0 0.61 27.8 5.4
19 (DB 533 x DB 534 F3 IPS 13) 32.6 47 3.3 0.66 27.7 5.9 20 (DB 533
x DB 534 F3 IPS 48) 31.4 45 3.7 0.69 27.7 6.0 21 (DB 533 x DB 534
F3 IPS 6) 33.0 46 3.7 0.67 27.6 5.8 22 (DB 533 x DB 534 F3 IPS 8)
37.1 43 3.1 0.61 27.6 5.6 23 (DB 533 x DB 534 F3 IPS 44) 32.37
48.40 3.99 0.70 27.40 6.07 24 (DB 533 x DB 534 F3 IPS 71) 39.37
47.70 3.25 0.64 25.97 5.93 25 (DB 533 x DB 534 F3 IPS 105) 34.60
45.83 3.80 0.69 25.67 6.00 26 (DB 533 x DB 534 F3 IPS 26) 36.97
46.40 3.55 0.67 25.53 6.00 27 (DB 533 x DB 534 F3 IPS 30) 37.77
47.87 3.32 0.63 25.50 5.87 28 (DB 533 x DB 534 F3 IPS 25) 31.77
47.20 3.61 0.66 25.13 6.13 Table 1. List of F3 barbadense parents
involved in the study of crossing and recombinational
variability
11. 2.2 Crossing Programme 2.3Evaluation of Derived F1s and F5
barbadense lines 2.4 Collection of data and recording of
observations Plant height (cm) Number of monopodia per plant Number
of sympodia per plant Number of bolls per plant Mean boll weight
(g) Reproductive points on sympodia Sympodial length at 50 per cent
plant height (cm) Inter branch distance (cm) Seed index (g) Ginning
outturn (%) Lint index (g) Seed cotton yield (kg ha-1)
Photosynthetic rate (molCO2m-2s-1) Stomatal conductance (molm-2s-1)
Transpiration rate (mmol H2Om-2s-1) 2.5% span length (mm) Fiber
uniformity ratio (%) Fiber micronaire value (g/inch) Fiber maturity
ratio (%) Fiber strength (g/tex) Fiber elongation (%)
12. Plate 4. Recording the physiological parameters by using
IRGA instrument
13. 2.5 Statistical Analysis 2.5.1 Combining ability analysis
2.5.1.1 Analysis of variance for combining ability Kempthorne
(1957) 2.5.1.2 ANOVA table outlines by Panse and Sukhatme (1961)
2.5.1.3 Line Tester analysis 2.5.1.4 Estimation of heterosis Turner
(1953) and Hayes et al. (1955) 2.5.1.5 Test of significant for
heterosis 2.5.1.6 Efficiency of tester Patil (1995) 2.5.1.7
Combining ability analysis Kempthorne (1957) 2.5.1.8 Estimates of
variances 2.5.1.9 Estimate of combining ability effects 2.5.1.10
Overall gca status of parents, sca status and heterosis of crosses
Mohan Rao (2001) 2.5.1.11 Pooled score for gca effects Arunachalam
and Bandopadhyay (1979) 2.5.1.12 Standard errors of the effects
2.5.1.13 Characterization of combining ability pattern (status) of
F4 lines Patil (1995) 2.5.1.14 Test of significance for genotypic
differences 2.5.1.15 Variance 2.5.1.16 Coefficient of variance (CV)
2.5.1.17 Correlation coefficient analysis Weber and Moorthy (1952)
2.5.1.18 Path coefficient analysis Wright (1921) 2.5.1.19
Intergeneration (F3-F5) correlation and regression analysis
2.5.1.20 Narrow sense heritability estimates Cahaner and Hillet
(1980)
14. The experimental results obtained in the present study are
presented under the following heads. Development of heterotic
groups (hirsutum vs barbadense) based on combining ability and
hybrid performance Evaluation of recombinational variability for
combining ability Molecular marker study in cotton In planta
genetic transformation
15. Development of heterotic groups (hirsutum vs barbadense)
based on combining ability and hybrid performance 1. Line x Tester
analysis (YHB hybrids) for confirmation of interspecific heterotic
groups
16. Table 3. Mean sum of squares for eight characters (RBD) in
interspecific YHB crosses Source d.f. Mean sum of squares Seed
cotton yield (kg/ha) Plant height (cm) No. of sympodia No. of bolls
Mean boll weight (g) Seed index (g) Ginning outturn (%) Lint index
(g) 1 2 3 4 5 6 7 8 Replications 1 1464781.85** 1722.35 198.14**
94.58** 2.29** 37.90** 12.28 1.85* Crosses 91 365118.66** 722.32
13.58 43.03** 0.23** 2.56** 13.51** 0.95** Error 91 47434.74 831.52
12.26 1.94 0.04 0.74 3.45 0.36 S.E (d) - 159.37 20.26 2.45 1.93
0.45 0.62 1.30 0.42 CD at 5% - 447.51 57.10 6.93 5.42 0.89 1.74
3.65 1.18 CD at 1% - 592.58 75.84 9.21 7.18 1.18 2.31 4.83 1.57 *
Significant at P = 0.05 ** Significant at P = 0.01
17. Sl. No. Crosses Seed cotton yield (kg/ha) Plant height (cm)
No. of monopodi a No. of sympodia No. of bolls Mean boll weight (g)
Reprodu ction Points on Sympodia Length of sympodia at 50% height
Inter Branch Distant (cm) Seed index (g) Ginning outturn (%) Lint
index (g) 2.5% SL (mm) % Micronaire value (g/inch) Maturity Ratio
(%) Tenacity (g/t) Elonga tion % Value Rank BMC 2 3 4 5 6 7 8 9 10
11 12 13 14 15 16 17 18 1 DH 18-31 x DB 533 3128.7 2 1 BMC 263.84
2.00 28.84 24.20 4.60 6.33 64.92 32.83 12.19 27.66 4.73 37.30 45.20
3.11 0.61 24.83 6.57 2 DH 49-1 x DB 533 2615.4 0 9 242.34 2.00
27.84 32.92 4.20 6.09 62.09 28.84 11.33 24.10 3.62 33.63 44.47 2.83
0.59 25.83 6.67 3 DH 23-1 x DB 533 2283.36 24 239.50 2.50 32.84
29.84 3.98 8.67 69.42 31.17 11.12 29.59 4.69 35.10 44.90 3.24 0.63
26.39 6.53 4 DH 98-27 x DB 533 2525.4 2 11 BMC 251.34 2.50 32.50
39.75 3.45 4.83 64.17 28.67 13.00 29.72 5.48 38.53 45.00 3.31 0.65
23.45 6.50 5 178-24 x DB 533 2487.6 4 13 BMC 244.83 1.84 32.00
31.59 4.23 5.25 56.42 27.84 12.55 34.46 6.64 38.27 42.50 2.87 0.59
23.88 6.57 6 DH46-1 x DB 533 2808.0 3 4 241.83 2.50 28.17 35.00
3.95 5.17 58.50 30.17 11.95 22.87 3.60 36.17 43.67 2.88 0.59 25.80
6.73 7 ZCH 8 x DB 533 2868.2 3 3 BMC 275.17 1.84 28.50 30.67 4.08
6.67 66.67 35.50 10.01 31.30 4.54 35.43 44.43 3.44 0.65 23.91 6.73
8 DH 37-4 x DB 533 2641.4 2 7 250.17 1.84 26.17 27.00 3.44 6.00
65.34 36.00 11.23 32.30 5.34 34.47 44.83 3.08 0.61 25.99 6.77 9 DH
24-4 x DB 533 2226.85 29 251.84 2.17 27.17 28.25 3.63 6.42 67.25
31.84 11.01 28.74 4.47 33.50 44.60 3.15 0.61 24.18 6.70 10 DH 8-7 x
DB 533 1597.88 71 257.67 1.84 24.34 26.67 3.57 6.67 69.00 33.84
10.45 28.27 4.14 33.67 46.57 3.18 0.62 26.13 6.90 11 DH 11-8 x DB
533 2083.37 41 259.84 1.84 27.17 29.17 3.72 5.75 65.84 30.50 13.96
27.27 5.21 40.20 42.97 2.39 0.56 24.66 6.20 12 DH 29-1 x DB 533
1657.66 68 263.34 1.84 31.33 36.50 3.85 6.50 73.25 29.34 12.00
25.85 4.21 32.93 45.03 2.55 0.57 28.92 6.67 13 DH 53-2 x DB 533
2477.78 15 248.50 2.00 31.17 28.42 4.03 5.67 60.50 29.50 12.11
29.85 5.13 35.40 41.33 2.95 0.60 24.22 6.50 14 DH 35-17 x DB 533
2105.27 39 272.67 2.00 26.50 37.17 3.48 5.92 64.84 30.67 12.84
25.30 4.39 36.47 44.70 3.02 0.61 25.34 6.53 15 DH 23-4 x DB 533
2252.76 26 206.84 2.17 22.33 21.59 3.28 6.75 54.42 27.67 13.00
25.43 4.46 38.73 44.10 3.15 0.63 22.68 6.73 16 DH 31-2 x DB 533
1659.60 67 224.34 2.00 26.34 22.92 3.10 5.83 65.67 29.34 12.95
29.15 5.37 34.77 43.83 2.90 0.60 24.70 6.63 17 DH 13-7 x DB 533
3047.6 2 2 257.67 1.84 27.34 40.25 3.54 7.59 71.33 31.83 12.17
30.73 5.44 31.90 41.70 3.26 0.62 23.57 6.57 18 DH 75- 23 x DB 533
1561.45 76 233.84 2.17 26.67 27.42 3.33 6.17 60.59 30.50 11.52
26.54 4.19 37.60 40.93 2.87 0.60 23.15 6.43 19 DH 91-1 x DB 533
2162.55 34 246.00 2.00 26.34 29.34 3.30 6.09 61.09 31.00 13.12
26.60 4.74 38.90 41.13 2.97 0.60 23.38 6.27 20 DH 45-23 x DB 533
2305.34 23 243.00 3.00 31.17 30.25 3.63 5.50 61.08 29.67 12.37
27.11 4.63 33.93 43.57 2.99 0.61 24.58 6.80 21 DH 14-2 x DB 533
1584.00 73 257.17 1.84 31.00 35.17 3.78 6.00 65.92 30.67 13.19
24.07 4.21 37.67 40.07 3.11 0.62 24.04 6.73 22 DH 82-3 x DB 533
2392.87 19 245.00 1.84 28.33 34.34 3.45 5.83 66.34 32.00 12.00
31.34 5.46 35.93 43.53 2.96 0.59 24.97 6.70 23 DH 23-21 x DB 533
1757.80 65 235.50 1.84 28.17 37.92 3.45 8.25 68.00 27.84 12.50
27.09 4.61 40.17 42.73 2.86 0.58 23.20 6.37 24 DH 18-31 x DB 532
2282.92 25 304.33 1.50 30.17 29.34 3.60 8.59 81.92 30.67 14.73
23.47 4.52 37.80 41.97 2.95 0.58 24.11 6.43 25 DH 49-1 x DB 532
2246.47 27 238.67 2.00 31.84 35.84 4.25 6.25 66.34 30.33 11.97
25.96 4.17 35.87 39.27 2.86 0.59 23.67 6.17 Table 4. Mean per se
performance of crosses in L x T study (YHB) in respect of seed
cotton yield and its attributing characters
21. Source d.f. Mean sum of squares Seed cotton yield (kg/ha)
Plant height (cm) No. of sympodi a No. of bolls Mean boll weight
(g) Seed index (g) Ginning outturn (%) Lint index (g) 1 2 3 4 5 6 7
8 Replications 1 1464781.85** 1722.35 198.14** 94.58** 2.29**
37.90** 12.28 1.85* Crosses 91 365118.66** 722.32 13.58 43.03**
0.23** 2.56** 13.51** 0.95** Line Effect 3 1344213.43** 705.20 0.99
56.37 0.61* 1.94 7.76 0.63 Tester Effect 22 903079.19** 541.25
11.19 45.34 0.28 3.86* 20.30* 1.46* Line x Tester Effect 66
141294.17** 783.46 14.95 41.65** 0.20** 2.16** 11.51** 0.80** Error
91 47434.74 831.52 12.26 1.94 0.04 0.74 3.45 0.36 Table 5.
Combining ability analysis of variance of crosses in L x T study
(YHB) in respect of seed cotton yield and its attributing
characters * Significant at P = 0.05 ** Significant at P =
0.01
22. Sl. No. 1 Characters Variance Due to GCA Variance Due to
SCA GCA/SCA Plant height (cm) 11.49800 6463.54050 0.00275 2 No. of
sympodia 0.01625 123.35975 0.00000 3 No. of bolls 0.91925 343.62825
0.00275 4 Mean boll Weight (g) 0.01025 1.65775 0.00675 5 Seed index
(g) 0.03150 17.79225 0.00175 6 Ginning outturn (%) 0.12650 94.93500
0.00100 7 Lint index (g) 0.01050 6.56300 0.00150 8 Seed cotton
yield (kg/ha) 21916.52625 1165676.92500 0.01700 Table 6. Variance
due to general and specific combining ability for seed cotton yield
and its attributing characters
24. Parents Seed cotton yield (kg/ha) Plant height (cm) No. of
sympodia No. of bolls Mean boll weight (g) Seed index (g) Ginning
outturn (%) Lint index (g) Value Rank 2 3 4 5 6 7 8 DH 75- 23
-557.05** 21 -21.358* -1.556 -2.63** 0.17* -1.047** 0.873 -0.21 DH
91-1 67.27 14 7.102 1.486 -2.38** -0.15* -0.192 -0.937 -0.297 DH
45-23 25.64 17 9.601 2.069 -1.23* 0.15* 1.169** -0.313 0.396 DH
14-2 -538.49** 20 4.769 0.611 0.93 -0.09 0.019 0.136 0.07 DH 82-3
142.20 13 -2.814 -0.349 -0.05 -0.28** -0.323 1.226 0.138 DH 23-21
-458.05** 18 1.643 1.028 5.18** -0.3** 0.513 1.447* 0.547* SE (gi)
77.00 10.20 1.24 0.49 0.07 0.30 0.66 0.21 SEd (gi-gj) 108.90 14.42
1.75 0.70 0.10 0.43 0.93 0.30 CD at 5% 215.62 20.25 2.46 1.38 0.21
0.61 1.30 0.42 CD at 1% 286.40 26.82 3.26 1.83 0.27 0.80 1.73 0.56
Barbadense Lines DB 533 242.92** 1 BMC 1.748 -0.118 -1.51** 0.00
-0.236 0.24 -0.027 DB 532 -4.92 3 1.146 0.194 0.52* -0.08* -0.014
-0.613* -0.14 DB 531 -313.54** 4 2.879 0.034 -0.07 -0.09** -0.015
0.15 0.025 DB 534 75.54* 2 BMC -5.774 -0.111 1.06** 0.16** 0.265*
0.23 0.142 SE (gi) 32.11 4.25 0.52 0.21 0.03 0.13 0.27 0.09 SEd
(gi-gj) 45.41 6.01 0.73 0.29 0.04 0.18 0.39 0.13 CD at 5% 89.92
8.45 1.03 0.58 0.09 0.25 0.54 0.18 CD at 1% 119.44 11.19 1.36 0.76
1.83 0.33 0.72 0.23 * Significant at P = 0.05 ** Significant at P =
0.01 BMC: Bench Mark Crosses
25. Testers gca effects Lines gca effects Fig 5. General
combining ability effects for lines and testers in respect of seed
cotton yield (kg/ha) in YHB crosses
26. Crosses Seed cotton yield (kg/ha) Plant height (cm) No. of
sympodia No. of bolls Mean boll weight (g) Seed index(g) Ginning
outturn)%( Lint index (g) 1 2 3 4 5 6 7 8 DH 18-31 x DB 533
528.15** 11.504 2.12 -3.7** 0.65** -0.93 0.532 -0.15 DH 49-1 x DB
533 179.83 -2.121 -0.757 0.86 0.16 -0.712 -1.797 -0.603 DH 23-1 x
DB 533 -20.16 -6.165 3.245 -1.78 0.24 -1.067 1.501 -0.068 DH 98-27
x DB 533 -78.91 10.045 2.578 8.47** -0.2 0.685 -1.212 -0.042 178-24
x DB 533 -19.9 -7.291 3.868 3.78** 0.41** 0.566 6.697** 1.914**
DH46-1 x DB 533 345* -0.168 1.205 4.26** 0.26 -0.512 -1.196 -0.35
ZCH 8 x DB 533 380.94* 20.004 -0.382 -2.49* 0.55** -0.704 1.947
0.089 DH 37-4 x DB 533 226.05 -4.333 -0.259 -5.76** -0.16 0.073
3.384* 0.777 DH 24-4 x DB 533 -301.61 4.835 0.2 -0.51 -0.06 -0.62
1.167 0.033 DH 8-7 x DB 533 -93.5 2.667 -4.13 -6.33** -0.27
-1.708** -0.418 -0.801 DH 11-8 x DB 533 -336.54* 30.795 0.45 -0.95
0.09 1.175 -1.774 -0.005 DH 29-1 x DB 533 -35.57 14.085 2.531
4.03** 0.01 -0.333 1.437 0.188 DH 53-2 x DB 533 149.72 2.71 3.031
-2.18* 0.16 0.69 0.957 0.47 DH 35-17 x DB 533 -473.08** 15.083
-3.381 0.74 0.17 1.53* -2.791* -0.042 DH 23-4 x DB 533 -288.1
-44.038* -5.635* -6.37** -0.26 0.232 -2.171 -0.445 DH 31-2 x DB 533
-124.58 -33.33 -2.299 -7.41** -0.77** 0.858 0.573 0.502 DH 13-7 x
DB 533 505.24** 14.292 -0.63 7.61** -0.23 -1.123 1.193 -0.152 DH
75- 23 x DB 533 -152.41 6.838 -0.134 -1.1 -0.53** 0.458 -2.388
-0.355 DH 91-1 x DB 533 -175.63 -9.457 -3.506 0.57 -0.25 1.203
-0.523 0.287 Table 8. Specific combining ability effects of derived
F1 hybrids for various characters included in Line x Tester
analysis
27. Crosses Seed cotton yield (kg/ha) Plant height (cm) No. of
sympodia No. of bolls Mean boll weight (g) Seed index(g) Ginning
outturn)%( Lint index (g) 1 2 3 4 5 6 7 8 DH 45-23 x DB 533 8.79
-14.956 0.741 0.34 -0.22 -0.913 -0.637 -0.516 DH 14-2 x DB 533
-148.42 4.04 2.034 3.09** 0.17 1.057 -4.126** -0.61 DH 82-3 x DB
533 -20.24 -0.541 0.324 3.24** 0.03 0.215 2.059 0.572 DH 23-21 x DB
533 -55.07 -14.498 -1.217 1.59 0.05 -0.122 -2.417 -0.692 DH 18-31 x
DB 532 -69.82 52.601* 3.139 -0.59 -0.27 1.388* -2.804* -0.241 DH
49-1 x DB 532 58.74 -5.189 2.931 1.76 0.29 -0.299 0.917 0.065 DH
23-1 x DB 532 177.96 -8.233 0.764 8.61** -0.09 0.366 0.289 0.155 DH
98-27 x DB 532 352.59* -13.358 -1.569 -3.72** 0.13 -1.092 1.387
-0.163 178-24 x DB 532 -67.85 10.811 -3.444 -0.66 0.26 -0.401
-1.859 -0.657 DH46-1 x DB 532 4.3 -17.226 -1.446 -4.85** -0.32*
-2.074** 0.683 -0.511 ZCH 8 x DB 532 311.09* 2.101 -1.694 -3.35**
-0.15 0.604 0.166 0.298 DH 37-4 x DB 532 -6.46 9.269 -0.57 4.21**
0.08 0.076 -3.507** -0.725 DH 24-4 x DB 532 155.18 -2.733 -0.276
0.3 -0.07 -0.547 1.441 0.097 DH 8-7 x DB 532 10.32 -15.226 0.724
-0.27 -0.15 -0.31 -1.889 -0.572 DH 11-8 x DB 532 -214.96 -10.938
-0.361 -5.56** 0.15 -0.782 2.999* 0.429 DH 29-1 x DB 532 -15.83
-25.313 -2.44 -3.16** -0.06 -0.105 -3.459** -0.743 DH 53-2 x DB 532
321.29* -8.858 -3.61 -0.21 -0.15 -0.257 1.936 0.314 DH 35-17 x DB
532 35.66 7.855 1.972 -1.45 0.13 -0.467 3.708** 0.627 DH 23-4 x DB
532 -276.01 1.729 0.389 5.27** 0.31* 0.85 2.303 0.899* DH 31-2 x DB
532 -64.25 24.772 5.055* 1.32 0.23 -0.814 -1.028 -0.565 DH 13-7 x
DB 532 -328.12* -15.936 0.889 -2.5* -0.17 2.04** -0.813 0.557
28. Crosses Seed cotton yield (kg/ha) Plant height (cm) No. of
sympodia No. of bolls Mean boll weight (g) Seed index(g) Ginning
outturn)%( Lint index (g) 1 2 3 4 5 6 7 8 DH 75- 23 x DB 532 -15.6
5.77 2.055 3.3** -0.11 -0.564 1.836 0.164 DH 91-1 x DB 532 136.03
1.645 -2.983 -3.04** 0.13 0.131 -2.514 -0.49 DH 45-23 x DB 532
-67.75 -1.354 0.765 -1.1 -0.27 0.59 3.727** 1.188** DH 14-2 x DB
532 -258.54 8.312 -1.943 -5.6** 0.12 -1.285* -1.327 -0.791 DH 82-3
x DB 532 -96.81 -14.439 -1.153 4.29** 0.23 0.343 -2.757* -0.52 DH
23-21 x DB 532 -81.17 13.939 2.806 6.98** -0.25 2.606** 0.567
1.187** DH 18-31 x DB 531 -285.7 -67.461** -5.202 6.08** 0.25
-0.841 2.236 0.234 DH 49-1 x DB 531 14.05 8.914 -0.574 -2.91**
-0.45** 0.398 0.002 0.13 DH 23-1 x DB 531 143.83 11.705 -1.242 0.36
-0.17 0.603 -1.921 -0.185 DH 98-27 x DB 531 -73.59 -15.92 -1.744
-3.31** -0.21 -0.231 -0.603 -0.258 178-24 x DB 531 345.95* -4.921
-1.954 -4.91** -0.13 -0.5 -4.999** -1.347** DH46-1 x DB 531 39.28
9.206 -2.452 1.82 -0.16 0.153 2.658* 0.644 ZCH 8 x DB 531 -198.08
-24.626 -1.199 4.32** -0.01 0.56 -1.654 -0.127 DH 37-4 x DB 531
-25.34 -21.294 -0.576 -0.95 0.05 0.338 -1.017 -0.11 DH 24-4 x DB
531 293.36 -2.295 -0.787 2.05* 0.05 0.494 0.061 0.222 DH 8-7 x DB
531 -48.61 5.536 1.213 -0.26 0.35* 0.957 1.341 0.698 DH 11-8 x DB
531 -13.86 -1.34 -0.532 0.61 -0.21 -1.511* 0.604 -0.451 DH 29-1 x
DB 531 -113.9 5.29 -0.451 -6.24** -0.19 -0.558 0.311 -0.148 DH 53-2
x DB 531 -456.72** -5.755 -1.116 -1.78 0.2 -0.176 -3.174* -0.756 DH
35-17 x DB 531 320.86* -11.047 2.297 8.22** -0.34* -0.706 1.073
-0.058 DH 23-4 x DB 531 1.94 30.161 1.718 0.45 -0.38* -0.313 1.068
0.124
29. Crosses Seed cotton yield (kg/ha) Plant height (cm) No. of
sympodia No. of bolls Mean boll weight (g) Seed index(g) Ginning
outturn)%( Lint index (g) 1 2 3 4 5 6 7 8 DH 31-2 x DB 531 44.88
43.87* 3.214 4.32** 0.27 0.468 -0.708 0 DH 13-7 x DB 531 -103.04
11.661 3.383 -2.16* 0.4** 0.047 -0.193 -0.018 DH 75- 23 x DB 531
-48.33 10.703 -1.451 -0.12 0.22 0.168 3.516** 0.874* DH 91-1 x DB
531 -56.65 -4.587 2.007 -0.37 -0.04 -0.577 0.776 -0.04 DH 45-23 x
DB 531 44.73 7.414 -0.241 0.07 0.29 1.272* -0.668 0.288 DH 14-2 x
DB 531 202.16 -9.085 1.882 4.82** -0.12 0.257 2.263 0.619 DH 82-3 x
DB 531 -133.61 12.499 3.342 -3.45** -0.25 0.934 -0.452 0.28 DH
23-21 x DB 531 106.39 11.371 0.466 -6.68** 0.57** -1.237* -0.518
-0.613 DH 18-31 x DB 534 -172.64 3.356 -0.057 -1.8 -0.63** 0.383
0.037 0.157 DH 49-1 x DB 534 -252.62 -1.604 -1.599 0.29 0 0.612
0.878 0.408 DH 23-1 x DB 534 -301.62 2.692 -2.767 -7.19** 0.03
0.097 0.13 0.098 DH 98-27 x DB 534 -200.1 19.232 0.736 -1.44 0.29
0.638 0.428 0.464 178-24 x DB 534 -258.19 1.401 1.531 1.79 -0.54**
0.335 0.162 0.09 DH46-1 x DB 534 -388.58* 8.189 2.693 -1.23 0.22
2.432** -2.146 0.217 ZCH 8 x DB 534 -493.95** 2.521 3.276 1.52
-0.39** -0.46 -0.458 -0.26 DH 37-4 x DB 534 -194.25 16.359 1.404
2.5* 0.02 -0.488 1.139 0.058 DH 24-4 x DB 534 -146.93 0.192 0.863
-1.83 0.07 0.673 -2.668* -0.351 DH 8-7 x DB 534 131.79 7.024 2.193
6.85** 0.07 1.061 0.967 0.675 DH 11-8 x DB 534 565.35** -18.518
0.443 5.9** -0.04 1.118 -1.83 0.027 DH 29-1 x DB 534 165.29 5.937
0.359 5.37** 0.23 0.996 1.712 0.704 DH 53-2 x DB 534 -14.29 11.902
1.694 4.17** -0.21 -0.257 0.282 -0.028
30. Crosses Seed cotton yield (kg/ha) Plant height (cm) No. of
sympodi a No. of bolls Mean boll weight (g) Seed index(g) Ginning
outturn )%( Lint index (g) 1 2 3 4 5 6 7 8 DH 35-17 x DB 534 116.56
-11.89 -0.888 -7.5** 0.04 -0.357 -1.991 -0.526 DH 23-4 x DB 534
562.18 ** 12.149 3.528 0.64 0.34* -0.769 -1.201 -0.578 DH 31-2 x DB
534 143.96 -35.313 -5.971* 1.77 0.27 -0.513 1.163 0.063 DH 13-7 x
DB 534 -74.07 -10.016 -3.642 -2.96** 0 -0.964 -0.187 -0.386 DH 75-
23 x DB 534 216.34 -23.31 -0.471 -2.08* 0.42** -0.063 -2.963*
-0.683 DH 91-1 x DB 534 96.26 12.4 4.482 2.83** 0.16 -0.758 2.262
0.243 DH 45-23 x DB 534 14.23 8.896 -1.266 0.69 0.21 -0.949 -2.422
-0.96* DH 14-2 x DB 534 204.8 -3.268 -1.973 -2.31* -0.16 -0.029
3.189* 0.782 DH 82-3 x DB 534 250.65 2.481 -2.513 -4.08** -0.01
-1.492* 1.149 -0.332 DH 23-21 x DB 534 29.84 -10.811 -2.054 -1.9
-0.38* -1.248* 2.368 0.119 SEd 154.00 20.39 2.48 0.99 0.15 0.61
1.31 0.42 CD at 5% 431.23 40.50 4.92 2.76 0.41 1.21 2.61 0.84 CD at
1% 572.80 53.65 6.51 3.67 0.55 1.60 3.46 1.12 * Significant at P =
0.05 ** Significant at P = 0.01
31. 2 .Mean per se performance of best HB interspecific
hybrids
32. Sl. No. Hybrids Seed cotton yield (kg/ha) Plant height (cm)
No. of mono podia No. of sym podia No. of bolls Mean boll weight
(g) Repro duction Points on Sympodia Length of sympo dia at 50%
height Inter Branch Distant (cm) Seed index (g) Ginning outturn (%)
Lint index (g) 2.5% SL (mm) % Micronaire value (g/inch) Tenacity
(g/t) Elon gation % Value Rank BMC 2 3 4 5 6 7 8 9 10 11 12 13 14
15 16 17 1 DH 102-23 x DB 534 1956.88 26 301.00 1.33 30.67 21.00
4.90 7.00 89.50 37.00 13.00 34.33 4.46 36.60 44.00 2.70 24.90 6.10
2 DH 39-84 x DB 531 1781.82 38 231.33 1.67 33.67 27.00 5.20 5.00
57.50 29.33 13.00 27.76 3.61 35.50 44.00 3.40 24.20 6.20 3 DH 22-76
x DB 534 1687.00 42 234.00 2.33 29.67 47.33 4.30 7.00 71.17 29.67
11.00 38.25 4.21 35.20 47.00 3.10 26.40 6.70 4 DH 57-95 x DB 533
1787.03 37 234.67 1.00 33.33 32.00 4.70 7.00 70.33 29.33 11.00
37.66 4.14 35.10 45.00 3.20 25.60 6.40 5 DH 11-8 x DB 532 1797.45
34 209.67 3.67 29.00 27.00 5.20 5.17 70.67 27.33 13.00 31.07 4.04
36.50 46.00 3.30 25.20 6.40 6 DH 28-36 x DB 531 1963.13 25 252.33
2.33 31.00 33.33 4.60 5.33 72.67 30.00 15.00 34.38 5.16 38.90 43.00
2.90 25.70 6.10 7 DH 98-27 x DB 533 2130.89 10 BMC 201.33 2.00
27.00 31.00 4.60 4.83 57.50 27.67 13.00 35.29 4.59 35.30 47.00 3.30
25.40 6.30 8 DH 18-31 x DB 532 1859.97 32 178.00 2.00 23.00 27.00
4.50 4.83 55.50 28.67 12.00 37.46 4.50 34.20 46.00 3.20 25.40 6.20
9 DH 47-67 x DB 532 1640.11 45 229.67 3.67 28.00 29.33 4.40 4.83
58.83 29.33 11.00 34.73 3.82 38.20 44.00 3.00 24.20 5.90 10 DH
40-29 x DB 532 2052.74 19 257.67 2.67 39.33 38.00 3.80 5.50 66.83
26.67 12.00 35.10 4.21 34.00 46.00 3.30 25.50 6.30 11 DH 18-31 x DB
533 2216.33 4 BMC 204.00 0.67 31.00 31.67 3.60 4.67 52.00 27.67
12.00 38.11 4.57 33.90 45.00 3.10 25.40 6.20 12 178-24 x DB 533
2151.73 8 BMC 258.67 1.33 34.00 34.00 4.30 6.50 63.67 30.00 12.00
34.54 4.14 36.40 45.00 3.00 24.90 5.90 13 DH 98-27 x DB 534 2239.26
2 BMC 236.00 1.67 28.00 21.67 5.10 6.50 66.83 29.33 13.00 36.12
4.70 35.40 47.00 3.00 27.30 6.30 14 DH 11-20 x DB 534 1232.69 49
202.67 2.67 30.33 27.33 4.30 6.17 62.00 27.67 12.00 34.31 4.12
38.90 44.00 2.80 25.70 6.00 15 DH 55-2 x DB 531 1795.37 35 223.33
1.33 29.00 35.33 4.20 6.67 70.67 34.67 14.00 36.04 5.05 36.20 46.00
2.60 25.60 6.00 16 ZCH 8 x DB 533 2277.81 1 BMC 227.00 2.00 29.33
30.00 4.70 4.50 53.33 28.33 11.00 34.64 3.81 35.60 44.00 3.20 26.50
6.40 17 DH 95- 18 x DB 533 2005.85 20 201.33 2.33 24.00 29.67 5.10
4.67 59.00 30.00 14.00 31.93 4.47 40.00 44.00 2.90 23.50 5.90 18 DH
53-2 x DB 534 1874.56 31 234.33 2.00 33.00 33.00 5.20 5.33 67.67
27.00 14.00 31.25 4.38 40.20 43.00 2.80 24.00 5.90 19 DH 43-44 x DB
532 1648.44 44 227.67 4.00 31.67 32.00 3.70 6.33 67.17 30.67 13.00
31.69 4.12 38.10 44.00 2.60 25.50 6.00 20 DH 53-2 x DB 533 1564.04
46 239.67 3.00 27.00 30.67 3.80 4.83 58.17 32.33 14.00 32.81 4.59
36.90 46.00 2.90 25.50 6.00 21 DH 18-31 x DB 534 2101.71 11 BMC
239.67 2.33 25.33 32.67 4.90 6.00 68.17 28.33 13.00 31.25 4.06
37.50 45.00 3.20 23.70 5.90 22 DH 21-29 x DB 532 1775.57 39 230.00
2.33 26.67 31.00 4.60 4.33 60.50 34.00 12.00 32.54 3.90 38.10 45.00
3.30 23.20 5.80 23 DH 25-22 x DB 533 1718.26 41 276.00 3.00 27.33
23.33 4.30 7.33 87.17 32.00 12.00 31.52 3.78 40.30 43.00 2.50 24.30
5.80 24 DH 18-19 x DB 533 1333.76 48 253.33 3.33 31.67 35.67 4.60
5.00 60.00 32.00 14.00 29.45 4.12 39.20 43.00 2.80 25.10 5.90 25 DH
29-32 x DB 531 1990.22 23 263.33 2.67 30.67 22.67 4.80 6.17 68.83
33.00 13.00 33.43 4.35 37.90 44.00 3.30 23.10 5.90 26 ZCH 8 x DB
532 1886.02 30 229.67 1.67 32.67 27.33 4.30 5.33 78.83 27.67 12.00
33.50 4.02 36.40 46.00 3.20 25.50 6.30 27 DH 11-10 x DB 534 2136.10
9 234.00 3.00 37.33 31.67 4.70 6.17 61.67 29.33 13.00 34.17 4.44
37.30 46.00 3.30 24.20 6.10 28 DH 66 x DB 534 1793.28 36 233.00
3.00 28.00 32.00 3.90 6.00 68.17 31.00 13.00 30.20 3.93 40.20 45.00
2.80 24.30 5.80 Table 9. The mean performance of best HB hybrids in
respect of seed cotton yield and its attributing characters