STMN2 Polyclonal antibodyCatalog Number:10586-1-AP Featured Product 13 Publications

For Research Use Only

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Basic Information Catalog Number:10586-1-AP

Size:150UL , Concentration: 180 μg/ml byBradford method using BSA as thestandard;

Source:Rabbit

Isotype:IgG

Immunogen Catalog Number:AG0929

GenBank Accession Number:BC006302

GeneID (NCBI):11075

Full Name:stathmin-like 2

Calculated MW:21 kDa

Observed MW:18-21 kDa

Purification Method:Antigen affinity purification

Recommended Dilutions:WB 1:1000-1:6000 IP 0.5-4.0 ug for IP and 1:500-1:2000for WBIHC 1:50-1:500 IF 1:10-1:100

Applications Tested Applications:IF, IHC, IP, WB, ELISA

Cited Applications:IF, IHC, WB

Species Specificity:human, mouse, rat

Cited Species:human, mouse, zebrafish

Note-IHC: suggested antigen retrieval withTE buffer pH 9.0; (*) Alternatively, antigenretrieval may be performed with citratebuffer pH 6.0

Positive Controls:

WB : rat brain tissue, mouse cerebellum tissue

IP : mouse brain tissue,

IHC : human medulloblastoma tissue, mouse braintissue

IF : SH-SY5Y cells, mouse brain tissue

Background Information Protein interacting with C kinase 1 (PICK1) was first cloned as a PKC-binding partner through yeast two hybridsystem. PICK1 acts as a critical regulator of membrane receptors'subcellular trafficking to modulate neuralprocesses such as learning and memory, and is widely expressed in brain, testis, heart, lung, liver, kidney andmuscle. It probably binds to and organize the subcellular localization of a variety of membrane proteins containingsome PDZ recognition sequence, for instance, PICK1 is a critical mediator of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) trafficking in neural synapses. PICK1 expression on D-serine release andglutamate transport in astrocytes suggests a potential implication of PICK1 in the progression of amyotrophiclateral sclerosis (ALS). PICK1 may also participate in breast cancer development through inhibition of TGF-betasignaling.

Notable Publications Author Pubmed ID Journal Application

Konstantin Feinberg 28877995 J Cell Biol WB

Adam Tuttle 31476133 Elife IHC

Jun Mukai 25913858 Neuron WB

Storage Storage:Store at -20°C. Stable for one year after shipment.Storage Buffer:PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Aliquoting is unnecessary for -20ºC storage

T: 1 (888) 4PTGLAB (1-888-478-4522) (toll freein USA), or 1(312) 455-8498 (outside USA)

E: proteintech@ptglab.com W: ptglab.com

For technical support and original validation data for this product please contact: This product is exclusively available under ProteintechGroup brand and is not available to purchase from anyother manufacturer.

Selected Validation Data

rat brain tissue were subjected to SDS PAGEfollowed by western blot with 10586-1-AP (STMN2antibody) at dilution of 1:3000 incubated at roomtemperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded human medulloblastoma tissue slideusing 10586-1-AP (STMN2 antibody) at dilution of1:200 (under 10x lens. Heat mediated antigenretrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human medulloblastoma tissue slideusing 10586-1-AP (STMN2 antibody) at dilution of1:200 (under 40x lens. Heat mediated antigenretrieval with Tris-EDTA buffer (pH 9.0).

Immunofluorescent analysis of (4% PFA) fixedmouse brain tissue using 10586-1-AP (STMN2antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IP Result of anti-STMN2 (IP:10586-1-AP, 3ug;Detection:10586-1-AP 1:1000) with mouse braintissue lysate 9500ug.

Immunofluorescent analysis of SH-SY5Y cells using10586-1-AP (STMN2 antibody) at dilution of 1:25and Alexa Fluor 488-conjugated AffiniPure GoatAnti-Rabbit IgG(H+L).