SAFER ALTERNATIVE TO EXPERIMENTAL GENE THERAPY AGAINST HIV INFECTION AND TOXINS
AND CELL DEATHManuela Gómez Londoño
Molecular biologyAugust 13 2012
UPB-Medellín
INTRODUCTION
ZFN is an artificial protein that binds to a short sequence of DNA and a DNA nuclease which cuts at the site of binding of ZFN, so cells cannot be easily repaired.
Cell death is good in case of DNA damage, mutations, exposition to carcinogens or radiation and is so bad in neurodegenerative disease or in cells wich cant be regenerated.
When ZFN gene enter to the cell
starts producing ZFN proteins wich
destroy specific genes of cell DNA.
Safer alternative to experimental gene therapy against HIV infection
The bad thing is that ZFN may act on other genes causing injury and damage to DNA, for this they use a transport protein of ZFN
Safer alternative to experimental gene therapy against HIV infection
ZFN gene therapy is use to interrupt the expression of
receptor CCR5 of T cells,creating high resistance
to virus.
Safer alternative to experimental gene therapy against HIV infection
This new technique with ZFN is too effective because its actue on specific gene causing
minimal damage to DNA.
PERSONAL COMMENT
This research is a big
advance in human
genetics, because since
from many years ago
have sought a cure for
HIV-AIDS with no results
and this new gene
therapy may promise a
cure.
Toxins induce apoptosis its start in the
mitochondria, where caspases enzymes are
activated by accumulation of reactive oxygen species (ROS).
Toxins and cell death
Apoptosis may be reversible if toxins are deleted quickly
of de cell and depending on the
type of cell and the time it was exposed to the toxin and if they are activated or not caspases.
Toxins and cell death
This process they
called anastasis:
: cell death
reversal process.
If thats happens
the cell begin to
repair self its DNA.
Toxins and cell death
Despite of the cells can be left with damage or a mutation because the enzymes that
cleave the AND are activated even though cell repair and fixes the most breaks of DNA.
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. This process is too useful for cells that do not have the ability to regenerate as neurons because with their death
the number reduces and will manifest different pathologies, so in these cells is better have some damage than to
be dead because with damages would do at least some of its functions
Medical utility New 1
Gene therapy has become the future of
medicine and hope for many conditions such
as regulating gene expression. we can take
control of life to the point of being able to avoid most diseases before they show up.
improving the quality of life of people.
Medical utility New 1
Gene therapy to make a zinc finger nuclease solution is a really cost effective because
it has the ability to act on specific genes and thus
ensures that the damage, DNA mutations and
alterations is minimal. So this is a real solution for HIV it is very likely that you can start
using in all medical fields
Medical utility New 2If the cells survive this
short exposure to toxins, will be a medical advance because it may arrest the
progression of neurodegenerative
diseases, may also be used in therapies against cancer, the quimioterapia
not would have to be continuous because it is toxic and it would help
reverse apoptosis
Medical utility New 2Anastasis is a big step in medicine because we may find a cure for cancer, something that has been sought for decades, since we could recover so that cells do not regenerate and can still perform their functions or some of them, thus allowing a person to have a better quality of life if you have some disease possibly reaching the cure.
BIBLIOGRAPY
Safer alternative to experimental gene therapy against HIV infection Published on July 2, 2012 at 9:30 AM NEW MEDICALS
Toxins and cell death: Interview with Professor Denise Montell Published on July 6, 2012 at 7:09 AM NEWS MEDICALS
MARTINEZ SÁNCHEZ, Lina María. Biología molecular.2. ed. Medellín: UPB. Fac. de Medicina, 2006. 208 p.