เนื้อเยื่อพืชPLANT TISSUES
Dr. Boonsatien Boonsoong
Department of ZoologyFaculty of Science
Kasetsart University
Objectives:
ศึกษาเปรียบเทียบลกัษณะที่แตกตางกันของพืชใบเลี้ยงเดี่ยวและพืชใบเลี้ยงคู
ศึกษาตําแหนงและการจัดเรียงตัวของเนื้อเยื่อและเซลลแตละชนิดในพืช
ศึกษาเนื้อเยื่อชนิดตางๆ ของพืชPLANT TISSUES
ฝกการเตรียมสไลดสดโดยวิธี free hand section
Plant growth:
primary tissue
• epidermis• cortex• primary xylem• primary phloem
vascular cambium
• secondary xylem• secondary phloem
secondary growthprimary growth
apical meristem lateral meristem
differentiate
cork cambium
periderm(cork, phelloderm)
differentiate differentiate
Stem growth:
Plant growth: Plant tissues: cell division
• Meristematic tissue
• Permanent tissue
• Apical meristem
• Lateral meristem: vascular cambium, cork cambium
• Intercalary meristem
• Simple permanent tissue: epidermis, cork, parenchyma, collenchyma, sclerenchyma
• Complex permanent tissue: xylem, phloem
Meristematic tissue:
Apical meristem
shoot apex root apex
Meristematic tissue:
Apical meristem
• Vascular cambium - สราง secondary xylem และ secondary phloem - Dicot
Meristematic tissue:
Lateral meristem
Meristematic tissue:
Lateral meristem (cont.)
• Cork cambium - สราง cork และ phelloderm
Meristematic tissue:
Intercalary meristem
Permanent tissue:
Cork
Parenchyma
Collenchyma
Sclerenchyma
Simple
Epidermis
dermal tissue, single layer of cells
• secretes a waxy layer called the cuticle that inhibits water loss
Simple permanent tissue:
Epidermis
guard cell
Simple permanent tissue:
Epidermis
non-living, water-resistant• protective tissue that displaces the stem epidermis
Simple permanent tissue:
Cork• healing and tissue regeneration• photosyntesis
Simple permanent tissue:
Parenchyma
• food storage
ChlorenchymaParenchyma
intercellularair space
Simple permanent tissue:
Parenchyma
palisade cell
spongy cell
Transverse section of leaf of common barberry (Berberis vulgaris)
• unevenly thick primary cell wall• support in young stems, roots, and petrioles
Simple permanent tissue:
Collenchyma
Transverse section of stem of angelica
Simple permanent tissue:
Collenchyma cells
Celery (Apium)
C.S.
L.S.
• dead at maturity• support and protection
Simple permanent tissue:
Sclerenchyma
• secondary cell wall
X-section L-section
Simple permanent tissue:
Sclerenchyma
Fiber Sclereid
Simple permanent tissue:
Sclereid
Pear (Pyrus)
Permanent tissue:
Complex
Cork
Parenchyma
Collenchyma
Sclerenchyma
Xylem
Phloem
Simple
Epidermis
Complex permanent tissue:
Vessel member
Tracheid
Xylem fiber
XYLEMXYLEM• Conduct waterand dissolvedminerals
• Support
Xylem parenchyma
Xylem:
Vessel Tracheid
• dead at maturity• end wall - perforation
• end wall - imperforation
• angiosperm• gymnosperm
Complex permanent tissue:
Phloem fiber
Sieve tubemember
Companioncell
• conduct food and other organic substances
Phloemparenchyma
PHLOEMPHLOEM
Phloem:
Sieve tube Companion cell
• sieve plate
Monocot & Dicot:
Monocot & Dicot:
Monocot stem, c.s. Dicot stem, c.s.
Stem monocot:
X - section
Stem monocot:
Vascular tissue
X - section
Stem dicot:
X - section
Epidermis
Cortex
Vascular bundle
Pith
Stem dicot: Stem dicot:
Epidermis
Cortex
Phloem
Pith
Xylem
Stem dicot:
Vascular tissue
X - section
Lab. session:
Meristem tissue
• L-S), Allium sp. root
• X-S, Coleussp. stem
Permanent tissue
• Epidermis
• Periderm
• Collenchyma
• Sclerenchyma
• Parenchyma
• Fiber
• Chlorenchyma
Monocot & Dicot
• Compare
• X-S, corn stem
• X-S, Coleus sp. stem
• Vascular bundle
Permanent Slide
Free hand sectioning:
Wet mount
• X-S, หญาละออง
• X-S, วานฝกดาบ
• X-S, ฝรั่ง
• กะลามะพราว• Safranin dye
• Aniline sulfate
Lab. results:
Fiber ของวานฝกดาบ
Safranin stained Aniline sulfate stained
Lab. results:
X-S หญาละออง
Vascular tissue, safranin stained
Lab. results:
Sclerenchyma ของฝรั่ง
Sclereid, safranin stained
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