SDChikungunya IgM
Distinguish Chikungunya from Dengue virus infection
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Rapid, Qualitative detection of Chikungunya IgM antibody
Immunochromatographic assay (ICA) rapid test
Fully covered device to prevent drying
Specimen : Serum, Plasma or Whole blood
Fast results : 10 minutes.
http://www.cdc.gov/ncidod/dvbid/Chikungunya/CH_Globalmap.html
Africa
Benin, Burundi, Cameroon, C. Africa, Congo DRC, Gabon, Guinea , Kenya , Madagascar, Malawi , Nigeria, Senegal, Seychelles, S. Africa, Tanzania, Uganda, Zimbabwe
Asia
Cambodia, India, Indonesia, Malaysia, Myanmar, Philippines, Singapore, Sri Lanka, Taiwan, Thailand, Vietnam
Europe Italy
Chikungunya and Dengue are among the most difficult diseases to distinguish, especially because
simultaneous co-infection can occur.
Why is important to distinguish Chikungunya from Dengue
Overlap and confusion with dengue fever.
--> The differential diagnosis of these two infections is essential for clinical managent and epidemiological study in the tropics.
The disease shares some clinical signs with dengue, and can be misdiagnosed in areas where dengue is common. Hence the number of cases of Chikungunya fever is much higher than that reported.
Chikungunya fever has similar symptoms but requires different management than dengue.
The word 'Chikungunya' is derived from the Makonde language in southeastern
Tanzania and means "bent down or become contorted," which indicates the
classic posture the patient adopts because of severe joint pain.
SD BIOLINE Chikungunya IgM
General Information
Chikungunya feverChikungunya fever is a viral disease transmitted through
the bite of infected Aedes aegypti and Aedes albopictus
mosquitoes.
Signs and symptomsAn abrupt onset of fever frequently accompanied by joint
pain, muscle pain, headache, nausea, fatigue and rash.
Incubation periodTime from the infected bite to the onset of illness can be
3-12 days, but is usually 4-7 days.
TreatmentCurrently specific drug treatment and vaccines are not
available.
Distinguish Chikungunya from Dengue virus infection
Approximate Global Distribution of Chikungunya Virus, by Country, 2008
Excellent performance
Internal Evaluation
Sensitivity : 97.1% (vs. ELISA)�
Specificity : 98.9% (vs. ELISA)
Clinical study
Chikungunya Case Definition ( WHO Recommended)
IgM antibody levels are highest three to five weeks after the onset of illness and persist for about two months.
Day-4 to day-2 Day 0 Day 4 to day 7 Day 15
Clinical symptoms
MosquitoBite
Early detection of IgM
Inoculation viral RNA IgMIgG
Laboratory criteria: at least one of the following tests in the acute phaseVirus isolationPresence of viral RNA by RT-PCRPresence of virus specific IgM antibodies in single serum sample collected in acute or convalescent stage.Four-fold rising of IgG titers in samples collected at least three weeks apart
Early detection is important in order to stop further transmission and case
management is necessary to prevent severe development of the disease.
Detection of Chikungunya IgM in acute phase
CHIK: Chikungunya
According to the research performed in southern Thailand(Chikungunya outbreak, 2008), SD BIOLINE Chikungunya IgM shows equivalent performance when compared to RT-PCR for collected CHIKV positive samples after onset of fever. RT-PCR for CHIKV was positive in every serum obtained from patients within the first three days after the onset of fever whereas the IgM antibody may become detectable after 3 days of the onset of fever. In this research, author emphasizes that serological or molecular methods are required to arrive at a definite diagnosis of either infection. RT-PCR and serology to detect IgM antibodies or paired sera for IgG against CHIKV ought to be performed in all patients with presumed hemorrhagic fever to detect outbreaks of CHIKV early. This precaution would help control global epidemics of this virus.
Specification
Test Procedure
Reference
Ordering Information
46FK10
Cat. No
Chikungunya IgM
Product
Serum/Plasma/ Whole blood
Specimen
Device
Type
1T x 25/Kit
Pack size Storage
1 drop(50l)
2 drops(100l)
1 drop
Negative
Positive
Invalid
Specimen : Serum, Plasma (50l) , Whole blood (100l)Assay Time : 10 minutesDetection : Chikungunya IgM antibodySensitivity : 97.1%, Specificity : 98.9% (vs. ELISA)Component : Test device/ Assay diluent/ Disposable droppers
http://www.searo.who.int/LinkFiles/Chikungunya_FactSheet_Chikungunyafever.pdfhttp://www.who.int/mediacentre/factsheets/fs327/en/http://www.searo.who.int/LinkFiles/Chikungunya_Draft_Prevention&Control_CHIK_25_Nov.pdfhttp://ecdc.europa.eu/pdf/071030CHK_mission_ITA.pdfhttp://www.cdc.gov/ncidod/dvbid/Chikungunya/CH_GlobalMap.html
24 months1~30 C
ELISA available
Rapid Test
46EK10
Rev. 1310-00C
at. No. LEF46EN
Cat. No
Chikungunya IgMELISA
Product
Serum
Specimen
Well
Type
96wells/Kit
Pack size Storage
12 months2~8 C
Whole Blood
Serum or Plasma
10 min.
65, Borahagal-ro, Giheung-gu, Yongin-si, Gyeonggi-do, Republic of KoreaTel : +82-31-899-9700 Fax : +82-31-899-9740E-mail : [email protected]
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