1
JP C
rud
e D
rug
Test
Product Name Wako Cat. No. Chemical Structural Formula
Achyranthes Root 017-22731
UnidentifiedCrude Drug Pkg. Size
Achyranthes root 5g
Grade Storage
for the JP Crude Drugs Test (for TLC) Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Aconitum Diester Alkaloids Standard 018-23741
Crude Drug Pkg. Size
Aconiti Rhizoma 0.35 mg
Grade Storage
for the JP Crude Drugs Test (for Purity Determination)
Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Aconitum Monoester Alkaloids Standard 014-23721
Crude Drug Pkg. Size
Aconiti Rhizoma 0.25 mg
Grade Storage
for the JP Crude Drugs Test (for Assay) Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Albiflorin, 98.0+ % (HPLC) 016-22201
C23H28O11 = 480.46CAS No. 39011-90-0
Crude Drug Pkg. Size
Paeoniae Radix 20 mg
Grade Storage
for the JP General Tests Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Alisol A, 97.0+ % (HPLC) 018-20681
C30H50O5 = 490.72CAS No. 19885-10-0
Crude Drug Pkg. Size
Alismatis Rhizoma 20 mg
Grade Storage
for the JP Crude Drugs Test (for TLC) Keep at -20℃.
for other products, please visit the Wako Online Catalog www.e-reagent.com
2
JP C
rud
e Dru
g Test
Product Name Wako Cat. No. Chemical Structural Formula
Amygdalin 017-23571
C20H27NO11 = 457.43CAS No. 29883-15-6
Crude Drug Pkg. Size
・Armeniacae Semen・Persicae Semen
20 mg
Grade Storage
for the JP Crude Drugs Test (for Assay and TLC)
Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Asarinin, 98.0+ % (HPLC) 012-24121
C20H18O6 = 354.35CAS No. 133-04-0
Crude Drug Pkg. Size
asiasarum root 10 mg
Grade Storage
for the JP Crude Drugs Test (for Thin-Layer Chromatography)
Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
(E)-Asarone, 98.0+ % (HPLC) 013-22571
C12H16O3 = 208.25CAS No. 2883-98-9
Crude Drug Pkg. Size
Perilla Herb 10 mg
Grade Storage
for the JP General Tests Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Astragaloside IV, 98.0+ % (HPLC) 015-20691
C41H68O14 = 784.97CAS No. 84687-43-4
Crude Drug Pkg. Size
Astragali Radix 20 mg
Grade Storage
for the JP Crude Drugs Test (for TLC) Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Atractylenolide III 018-24461
C15H20O3 = 248.32CAS No. 73030-71-4
Crude Drug Pkg. Size
Atractylodes rhizome 20 mg
Grade Storage
for the JP Crude Drugs Test (for Assay and TLC)
Keep at 2-10℃.
3
JP C
rud
e D
rug
Test
Product Name Wako Cat. No. Chemical Structural Formula
Atractylodin TS (5.0 mg/L in methanol) 015-24471
C13H10O = 182.22CAS No. 55290-63-6
Crude Drug Pkg. Size
Atractylodes rhizome 1 mL x 5A
Grade Storage
for the JP Crude Drugs Test (for Assay) Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Atropine Sulfate Hydrate, 99.0+ % (HPLC) 011-23731
(C17H23NO3)2・H2SO4・H2O = 694.83CAS No. 5908-99-6
Crude Drug Pkg. Size
・Belladonnae Radix・Ephedra Herb・Scopolia Rhizome
50 mg
Grade Storage
for the JP Crude Drugs Test (for Assay and TLC)
Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Arbutin 019-23771
C12H16O7 = 272.25
Crude Drug Pkg. Size
Uvae Ursi Folium 20 mg
Grade Storage
for the JP Crude Drugs Test (for Assay and TLC)
Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Artemisia Argyi 018-24221
UnidentifiedCrude Drug Pkg. Size
Artemisiae Folium 10g
Grade Storage
for the JP Crude Drugs Test (for Purity Determination)
Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Baicalin, 98.0+ % (HPLC) 024-15691
C21H18O11・H2O = 464.38CAS No. 21967-41-9
Crude Drug Pkg. Size
Scutellariae Radix 20 mg
Grade Storage
for the JP Crude Drugs Test (for TLC) Keep at 2-10℃.
for other products, please visit the Wako Online Catalog www.e-reagent.com
4
JP C
rud
e Dru
g Test
Product Name Wako Cat. No. Chemical Structural Formula
Barbaloin 022-16971
C21H22O9 = 418.39CAS No. 1415-73-2
Crude Drug Pkg. Size
Aloe 50 mg
Grade Storage
for the JP Crude Drugs Test (for Assay and TLC)
Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Benz [a] anthracene 023-16761
C18H12 = 228.29CAS No. 56-55-3
Crude Drug Pkg. Size
Wood-tar creosote 20 mg
Grade Storage
for the JP General Tests Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Benzo [a] pyrene 026-16751
C20H12 = 252.31CAS No. 50-32-8
Crude Drug Pkg. Size
Wood-tar creosote 20 mg
Grade Storage
for the JP General Tests Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Benzoylmesaconine Hydrochloride 022-16611
C31H43NO10・HCl = 626.13
Crude Drug Pkg. Size
Aconiti Rhizoma 5 mg
Grade Storage
for the JP Crude Drugs Test (for Assay and TLC)
Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Berberine Chloride Hydrate, 95.0 - 102.0 % (calculated on the dehydrous basis)
025-17181
C20H18ClNO4 = 371.81CAS No. 141433-60-5
Crude Drug Pkg. Size
・Corydalis Tuber・Phellodendri Cortex・Coptidis Rhizoma
20 mg
Grade Storage
for the JP Crude Drugs Test (for TLC) Keep at 2-10℃.
5
JP C
rud
e D
rug
Test
Product Name Wako Cat. No. Chemical Structural Formula
Bergenin, 99.0+ % (HPLC) 027-16421
C14H16O9 = 328.27CAS No. 477-90-7
Crude Drug Pkg. Size
Mallotus japonicus (Thunb.) Muell. Arg. 20 mg
Grade Storage
for the JP Crude Drugs Test (for TLC) Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Bisdemethoxycurcumin 020-16411
C19H16O4 = 308.33CAS No. 33171-05-0
Crude Drug Pkg. Size
Curcuma longa L. 10 mg
Grade Storage
for the JP General Tests Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Bufalin 029-16981
C24H34O4 = 386.52CAS No. 465-21-4
Crude Drug Pkg. Size
Bufo bufo gargarizans Cantor 20 mg
Grade Storage
for the JP Crude Drugs Test (for Assay) Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
(E)-Capsaicin 034-21121
C18H27NO3 = 305.41CAS No. 404-86-4
Crude Drug Pkg. Size
Capsici Fructus 20 mg
Grade Storage
for the JP Crude Drugs Test (for Assay and TLC)
Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Chikusetsusaponin IV, 98.0+ % (HPLC) 035-23091
C47H74O18 = 927.08CAS No. 7518-22-1
Crude Drug Pkg. Size
Panax Rhizome 10 mg
Grade Storage
for the JP Crude Drugs Test (for TLC) Keep at 2-10℃.
for other products, please visit the Wako Online Catalog www.e-reagent.com
6
JP C
rud
e Dru
g Test
Product Name Wako Cat. No. Chemical Structural Formula
(E)-Chlorogenic Acid, 98.0+ % (HPLC) 039-20331
C16H18O9 = 354.31CAS No. 327-97-9
Crude Drug Pkg. Size
Lonicerae Folium Cum Caulis 10 mg
Grade Storage
for the JP Crude Drugs Test (for TLC) Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
(E)-Cinnamaldehyde, 98.0+ % (HPLC) 034-21981
This product may be deteriorated phototermally, so please use it in a cool dark place.
C9H8O = 132.16CAS No. 14371-10-9
Crude Drug Pkg. Size
Cinnamomi Cortex 1 mL x 5
Grade Storage
for the JP Crude Drugs Test (for TLC) Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
(E)-Cinnamic Acid 037-22211
This product may be deteriorated phototermally, so please use it in a cool dark place.
C9H8O2 = 148.16CAS No. 140-10-3
Crude Drug Pkg. Size
Cinnamomi Cortex 20 mg
Grade Storage
for the JP Crude Drugs Test (for Assay and TLC)
Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Cinobufagin, 98.0+ % (JP method) 037-21971
C26H34O6 = 442.54CAS No. 470-37-1
Crude Drug Pkg. Size
Bufo bufo gargarizans Cantor 20 mg
Grade Storage
for the JP Crude Drugs Test (for Assay) Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Coptisine Chloride, 98.0+ % (HPLC) 038-22001
C19H14NO4Cl = 355.77CAS No. 6020-18-4
Crude Drug Pkg. Size
Coptidis Rhizoma 10 mg
Grade Storage
for the JP Crude Drugs Test (for TLC) Keep at 2-10℃.
7
JP C
rud
e D
rug
Test
Product Name Wako Cat. No. Chemical Structural Formula
Curcumin 031-21991
C21H20O6 = 368.38CAS No. 458-37-7
Crude Drug Pkg. Size
Curcuma longa L. 10 mg
Grade Storage
for the JP Crude Drugs Test (for Assay) Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Cycloartenyl Ferulate, 98.0+ % (HPLC) 035-22011
C40H58O4 = 602.89CAS No. 21238-33-5
Crude Drug Pkg. Size
Oryzae Semen 10 mg
Grade Storage
for the JP Crude Drugs Test (for TLC) Keep at -20℃.
Product Name Wako Cat. No. Chemical Structural Formula
Dehydrocorydaline Nitrate 045-32941
C22H24N2O7 = 428.44CAS No. 13005-09-9
Crude Drug Pkg. Size
Corydalis Tuber 20 mg
Grade Storage
for the JP Crude Drugs Test (for Assay and TLC)
Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Demethoxycurcumin 049-31001
C20H18O5 = 338.35CAS No. 22608-11-3
Crude Drug Pkg. Size
Curcuma longa L. 10 mg
Grade Storage
for the JP General Tests Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Eleutheroside B 051-07551
C17H24O9 = 372.37CAS No. 118-34-3
Crude Drug Pkg. Size
Siberian Ginseng 20 mg
Grade Storage
for the JP Crude Drugs Test (for LC) Keep at 2-10℃.
for other products, please visit the Wako Online Catalog www.e-reagent.com
8
JP C
rud
e Dru
g Test
Product Name Wako Cat. No. Chemical Structural Formula
(E)-Ferulic Acid 067-06091
C10H10O4 = 194.18CAS No. 537-98-4
Crude Drug Pkg. Size
・Angelicae Radix・Cimicifugae Rhizoma
20 mg
Grade Storage
for the JP Crude Drugs Test (for Assay) Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Fructose, 99.0+ % (HPLC) 060-06081
C6H12O6 = 180.16CAS No. 57-48-7
Crude Drug Pkg. Size
Rehmanniae Radix 20 mg
Grade Storage
for the JP Crude Drugs Test (for TLC) Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Geniposide, 95.0+ % (qNMR) 073-05891
C17H24O10 = 388.37CAS No. 24512-63-8
Crude Drug Pkg. Size
Gardeniae Fructus 20 mg
Grade Storage
for the JP Crude Drugs Test (for Assay and TLC)
Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Gentiopicroside, 98.0+ % (HPLC) 073-05031
C16H20O9 = 356.32CAS No. 20831-76-9
Crude Drug Pkg. Size
・Gentianae Radix・Gentianae Scabrae Radix
10 mg
Grade Storage
for the JP Crude Drugs Test (for TLC) Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
[6]-Gingerol 076-05901
C17H26O4 = 294.39
Crude Drug Pkg. Size
Zingiberis Rhizoma 20 mg
Grade Storage
for the JP Crude Drugs Test (for Assay and TLC)
Keep at 2-10℃.
9
JP C
rud
e D
rug
Test
Product Name Wako Cat. No. Chemical Structural Formula
Ginsenoside Rc, 98.0+ % (HPLC) 075-05231
C53H90O22 = 1079.27CAS No. 11021-14-0
Crude Drug Pkg. Size
・Ginseng Radix Rubra・Panacis Ginseng Radix
20 mg
Grade Storage
for the JP General Tests Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Ginsenoside Re, 99.0+ % (HPLC) 072-05241
C48H82O18 = 947.15CAS No. 52286-59-6
Crude Drug Pkg. Size
・Ginseng Radix Rubra・Panacis Ginseng Radix
20 mg
Grade Storage
for the JP General Tests Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Ginsenoside-Rg1, 99.0+ % (HPLC) 076-05021
C42H72O14 = 801.01CAS No. 22427-39-0
Crude Drug Pkg. Size
・Ginseng Radix Rubra・Panacis Ginseng Radix
10 mg
Grade Storage
for the JP Crude Drugs Test (for TLC) Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
4'-O-Glucosyl-5-O-methylvisamminol, 98.0+ % (HPLC)
076-05761
C22H28O10 = 452.45CAS No. 84272-85-5
Crude Drug Pkg. Size
Glehniae Radix cum Rhizoma 5 mg
Grade Storage
for the JP Crude Drugs Test (for TLC) Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Glycyrrhizic Acid, 99.0+ % (HPLC) 070-05161
C42H62O16 = 822.93CAS No. 1405-86-3
Crude Drug Pkg. Size
Glycyrrhizae Radix 20 mg
Grade Storage
for the JP Crude Drugs Test (for TLC) Keep at 2-10℃.
for other products, please visit the Wako Online Catalog www.e-reagent.com
10
JP C
rud
e Dru
g Test
Product Name Wako Cat. No. Chemical Structural Formula
Guaiacol 073-05771
C7H8O2 = 124.14CAS No. 90-05-1
Crude Drug Pkg. Size
Wood-tar creosote 500 mg
Grade Storage
for the JP General Tests (for Determination) Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Hesperidin 085-09311
C28H34O15 = 610.56CAS No. 520-26-3
Crude Drug Pkg. Size
Aurantii Nobilis Pericarpium 10 mg
Grade Storage
for the JP Crude Drugs Test (for Assay and TLC)
Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Hirsutine 085-09291
C22H28N2O3 = 368.47CAS No. 7729-23-9
Crude Drug Pkg. Size
Uncariae Uncis Cum Ramulus 5 mg
Grade Storage
for the JP Crude Drugs Test (for Assay and TLC)
Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Honokiol, 99.0+ % (HPLC) 083-08511
C18H18O2 = 266.33CAS No. 35354-74-6
Crude Drug Pkg. Size
Magnoliae Cortex 20 mg
Grade Storage
for the JP General Tests Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
10-Hydroxy-2-(E)-decenoic Acid 081-09271
C10H18O3 = 186.25CAS No. 14113-05-4
Crude Drug Pkg. Size
Royal jelly 20 mg
Grade Storage
for the JP Crude Drugs Test (for Assay and TLC)
Keep at 2-10℃.
11
JP C
rud
e D
rug
Test
Product Name Wako Cat. No. Chemical Structural Formula
3-(3-Hydroxy-4-methoxyphenyl)-2-(E)-propenoic Acid, 98.0+ % (HPLC)
085-08691
C10H10O4 = 194.18CAS No. 537-73-5
Crude Drug Pkg. Size
Cimicifugae Rhizoma 20 mg
Grade Storage
for the JP General Tests Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Hyodeoxycholic Acid, 98.0+ % (HPLC) 089-09211
C24H40O4 = 392.57CAS No. 83-49-8
Crude Drug Pkg. Size
・Powdered Swine Bile・Fel Ursi
50 mg
Grade Storage
for the JP Crude Drugs Test (for TLC) Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Hyperoside 087-08651
C21H20O12 = 464.38CAS No. 482-36-0
Crude Drug Pkg. Size
Crataegi Fructus 10 mg
Grade Storage
for the JP Crude Drugs Test (for TLC) Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Icariin, 99.0+ % (HPLC) 094-05281
C33H40O15 = 676.66CAS No. 489-32-7
Crude Drug Pkg. Size
Epimedii Herba 20 mg
Grade Storage
for the JP Crude Drugs Test (for TLC) Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Limonin, 98.0+ % (HPLC) 123-06101
C26H30O8 = 470.51CAS No. 1180-71-8
Crude Drug Pkg. Size
Phellodendri Cortex 10 mg
Grade Storage
for the JP Crude Drugs Test (for TLC) Keep at 2-10℃.
for other products, please visit the Wako Online Catalog www.e-reagent.com
12
JP C
rud
e Dru
g Test
Product Name Wako Cat. No. Chemical Structural Formula
Liquiritin, 98.0+ % (HPLC) 129-05341
C21H22O9 = 418.39CAS No. 551-15-5 (anhy)
Crude Drug Pkg. Size
Glycyrrhizae Radix 20 mg
Grade Storage
for the JP Crude Drugs Test (for TLC) Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Loganin 128-05931
C17H26O10 = 390.38CAS No. 18524-94-2
Crude Drug Pkg. Size
・Corni Fructus・Lonicerae Folium Cum Caulis
20 mg
Grade Storage
for the JP Crude Drugs Test (for Assay and TLC)
Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Luteolin, 98.0+ % (HPLC) 125-05441
C15H10O6 = 286.24CAS No. 491-70-3
Crude Drug Pkg. Size
Chrysanthemi Flos 10 mg
Grade Storage
for the JP Crude Drugs Test (for TLC) Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Magnolol, 98.0+ % (qNMR) 130-16781
C18H18O2 = 266.33CAS No. 528-43-8
Crude Drug Pkg. Size
Magnoliae Cortex 20 mg
Grade Storage
for the JP Crude Drugs Test (for Assay and TLC)
Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Manninotriose, 98.0+ % (HPLC) 135-17711
C18H32O16 = 504.44CAS No. 13382-86-0
Crude Drug Pkg. Size
Rehmanniae Radix 10 mg
Grade Storage
for the JP Crude Drugs Test (for TLC) Keep at 2-10℃.
13
JP C
rud
e D
rug
Test
Product Name Wako Cat. No. Chemical Structural Formula
l-Menthol, 99.0+ % (JP method) 132-16562
C10H20O = 156.27CAS No. 2216-51-5
Crude Drug Pkg. Size
Menthae Herba 25g
Grade Storage
for the JP General Tests(for Determination) Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
(E)-2-Methoxycinnamaldehyde,98.0+ % (HPLC)
134-16181
C10H10O2 = 162.19CAS No. 1504-74-1
Crude Drug Pkg. Size
Cinnamomi Cortex 20 mg
Grade Storage
for the JP Crude Drugs Test (for TLC) Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Myristicin, 98.0+ % (HPLC) 137-16291
C11H12O3 = 192.21CAS No. 607-91-0
Crude Drug Pkg. Size
Myristica fragrans 20 mg
Grade Storage
for the JP Crude Drugs Test (for TLC) Keep at -20℃.
Product Name Wako Cat. No. Chemical Structural Formula
Naringin, 99.0+ % (HPLC) 149-07901
C27H32O14 = 616.57CAS No. 10236-47-2
Crude Drug Pkg. Size
・Rhei Rhizoma・Aurantii Nobilis Pericarpium・Aurantii Pericarpium
20 mg
Grade Storage
for the JP Crude Drugs Test (for TLC) Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Nodakenin, 98.0+ % (HPLC) 148-08571
C20H24O9 = 408.40CAS No. 495-31-8
Crude Drug Pkg. Size
Peucedani Radix 5 mg
Grade Storage
for the JP Crude Drugs Test (for TLC) Keep at 2-10℃.
for other products, please visit the Wako Online Catalog www.e-reagent.com
14
JP C
rud
e Dru
g Test
Product Name Wako Cat. No. Chemical Structural Formula
Osthole, 99.0+ % (HPLC) 151-02641
C15H16O3 = 244.29CAS No. 484-12-8
Crude Drug Pkg. Size
Cnidii Monnieri Fructus 20 mg
Grade Storage
for the JP Crude Drugs Test (for TLC) Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Paeoniflorin, 98.0+ % (HPLC) 164-21731
C23H28O11 = 480.46CAS No. 23180-57-6
Crude Drug Pkg. Size
Paeoniae Radix 10 mg
Grade Storage
for the JP Crude Drugs Test (for TLC) Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Paeonol, 98.0+ % (qNMR) 164-24891
C9H10O3 = 166.17CAS No. 552-41-0
Crude Drug Pkg. Size
Paeoniae Moutan Cortex 10 mg
Grade Storage
for the JP Crude Drugs Test (for Assay and TLC)
Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Palmatine Chloride, 99.0+ % (HPLC) 165-22481
C21H22ClNO4 = 387.86CAS No. 10605-02-4
Crude Drug Pkg. Size
Phellodendri Cortex 20 mg
Grade Storage
for the JP General Tests Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Perillaldehyde 161-24161
C10H14O = 150.22CAS No. 2111-75-3
Crude Drug Pkg. Size
Perillae Herba 30 mg× 5A
Grade Storage
for the JP Crude Drugs Test (for Assay and TLC)
Keep at 2-10℃.
15
JP C
rud
e D
rug
Test
Product Name Wako Cat. No. Chemical Structural Formula
Plantago Seed 168-24171
UnidentifiedCrude Drug Pkg. Size
Plantaginis Semen 10g
Grade Storage
for the JP Crude Drugs Test (for TLC) Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Powdered Swine Bile 168-24311
UnidentifiedCrude Drug Pkg. Size
Fel Ursi 1g
Grade Storage
for the JP Crude Drugs Test (for TLC) Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
(±)-Praeruptorin A, 98.0+ % (HPLC) 165-25281
C21H22O7 = 386.40CAS No. 73069-25-7
Crude Drug Pkg. Size
Peucedani Radix 10 mg
Grade Storage
for the JP Crude Drugs Test (for TLC) Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Puerarin, 98.0+ % (HPLC) 165-22001
C21H20O9 = 416.38CAS No. 3681-99-0
Crude Drug Pkg. Size
Puerariae Radix 20 mg
Grade Storage
for the JP Crude Drugs Test (for TLC) Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Resibufogenin, 98.0+ % (JP method) 185-02681
C24H32O4 = 384.51CAS No. 465-39-4
Crude Drug Pkg. Size
Bufo bufo gargarizans Cantor 20 mg
Grade Storage
for the JP Crude Drugs Test (for Assay and TLC)
Keep at 2-10℃.
for other products, please visit the Wako Online Catalog www.e-reagent.com
16
JP C
rud
e Dru
g Test
Product Name Wako Cat. No. Chemical Structural Formula
Rhein, 98.0+ % (HPLC) 180-02251
C15H8O6 = 284.22CAS No. 478-43-3
Crude Drug Pkg. Size
Rhei Rhizoma 10 mg
Grade Storage
for the JP Crude Drugs Test (for TLC) Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Rhynchophylline 188-02671
C22H28N2O4 = 384.47CAS No. 76-66-4
Crude Drug Pkg. Size
Uncariae Uncis Cum Ramulus 10 mg
Grade Storage
for the JP Crude Drugs Test (for Assay and TLC)
Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Rosmarinic Acid 181-02661
C18H16O8 = 360.31CAS No. 20283-92-5
Crude Drug Pkg. Size
Perillae Herba 20 mg
Grade Storage
for the JP Crude Drugs Test (for Assay and TLC)
Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Saikosaponin a 192-16281
C42H68O13 = 780.98CAS No. 20736-09-8
Crude Drug Pkg. Size
Bupleuri Radix 25 mg
Grade Storage
for the JP Crude Drugs Test (for Assay and TLC)
Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Saikosaponin b2 199-16171
C42H68O13 = 780.98CAS No. 58316-41-9
Crude Drug Pkg. Size
Bupleuri Radix 20 mg
Grade Storage
for the JP Crude Drugs Test (for Assay and TLC)
Keep at 2-10℃.
17
JP C
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Test
Product Name Wako Cat. No. Chemical Structural Formula
Saikosaponin d 199-16311
C42H68O13 = 780.98CAS No. 20874-52-6
Crude Drug Pkg. Size
Bupleuri Radix 20 mg
Grade Storage
for the JP Crude Drugs Test (for Assay) Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Schizandrin, 99.0+ % (HPLC) 197-13551
C24H32O7 = 432.51CAS No. 7432-28-2
Crude Drug Pkg. Size
Schisandrae Fructus 20 mg
Grade Storage
for the JP Crude Drugs Test (for TLC) Keep at RT.
Product Name Wako Cat. No. Chemical Structural Formula
Scopoletin, 98.0+ % (HPLC) 191-16751
C10H8O4 = 192.17CAS No. 92-61-5
Crude Drug Pkg. Size
Artemisiae Folium 20 mg
Grade Storage
for the JP Crude Drugs Test (for TLC) Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Sesamin, 98.0+ % (HPLC) 197-16231
C20H18O6 = 354.35CAS No. 607-80-7
Crude Drug Pkg. Size
Sesami Semen 20 mg
Grade Storage
for the JP Crude Drugs Test (for TLC) Keep at -20℃.
Product Name Wako Cat. No. Chemical Structural Formula
[6]-Shogaol 192-16161
C17H24O3 = 276.37CAS No. 555-66-8
Crude Drug Pkg. Size
Zingiberis Siccatum Rhizoma 10 mg
Grade Storage
for the JP Crude Drugs Test (for Assay and TLC)
Keep at 2-10℃.
for other products, please visit the Wako Online Catalog www.e-reagent.com
18
JP C
rud
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g Test
Product Name Wako Cat. No. Chemical Structural Formula
Sodium Glycocholate, 98.0+ % (HPLC) 197-15491
C26H42NNaO6 = 487.60CAS No. 863-57-0
Crude Drug Pkg. Size
Fel Ursi 20 mg
Grade Storage
for the JP Crude Drugs Test (for TLC) Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Sodium Tauroursodeoxycholate, 98.0+ % (HPLC)
190-15501
C26H44NNaO6S = 521.69CAS No. 35807-85-3
Crude Drug Pkg. Size
Fel Ursi 20 mg
Grade Storage
for the JP Crude Drugs Test (for TLC) Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Stachyose, 99.0+ % (HPLC) 195-16911
C24H42O21 = 666.58CAS No. 470-55-3
Crude Drug Pkg. Size
Rehmanniae Radix 20 mg
Grade Storage
for the JP Crude Drugs Test (for TLC) Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Swertiamarin, 99.0+ % (HPLC) 191-13951
C16H22O10 = 374.34CAS No. 17388-39-5
Crude Drug Pkg. Size
Swertia Herb 20 mg
Grade Storage
for the JP Crude Drugs Test (for TLC) Keep at 2-10℃.
Product Name Chemical Structural Formula
Theophylline
C7H8N4O2 = 180.16CAS No. 58-55-9
Crude Drug
Swertia Herb
Grade Storage
for the JP Crude Drugs Test (for Determination) Keep at 2-10℃.
Wako Cat. No.
203-18281 209-18283
Pkg. Size
100mg 1 g
19
JP C
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Test
Product Name Wako Cat. No. Chemical Structural Formula
Umbelliferone, 98.0+ % (HPLC) 212-01461
C9H6O3 = 162.14CAS No. 93-35-6
Crude Drug Pkg. Size
Artemisiae Folium 20 mg
Grade Storage
for the JP Crude Drugs Test (for TLC) Keep at 2-10℃.
Product Name Wako Cat. No. Chemical Structural Formula
Wogonin, 98.0+ % (HPLC) 236-02321
C16H12O5 = 284.26CAS No. 632-85-9
Crude Drug Pkg. Size
Scutellariae Radix 10 mg
Grade Storage
for the JP Crude Drugs Test (for TLC) Keep at 2-10℃.
Functional group Packing material name Column SizeWako Cat. No.
Purity of silica gelD (DuPont type) W (Waters type)
Separation mode: Partition
ODS (C30) Navi C30-5 4.6 φ x 150 mm 237-61191 233-61193
High purity
ODS (C18) Navi C18-5 4.6 φ x 150 mm 231-60511 237-60513
Wakosil-II 5C18 4.6 φ x 150 mm 230-51811 236-51813
Wakosil-II 5C18 AR 4.6 φ x 150 mm 238-50751 234-50753
Wakosil-II 5C18 HG 4.6 φ x 150 mm 238-51111 234-51113
Wakosil-II 5C18 RS 4.6 φ x 150 mm 238-51471 234-51473
Wakosil 5C184.6 φ x 150 mm 233-53981 239-53983
Normal4.6 φ x 250 mm 237-54001 233-54003
Wakosil 5C18-200 4.6 φ x 250 mm 235-55521 231-55523
Ph (phenyl) Wakosil 5Ph 4.6 φ x 250 mm 230-57411 236-57413
Product Name Package Size Wako Cat. No. Grade
Silicagel 70 Plate Wako 100 sheets ( 5 × 20 cm) 197-08384
for Thin-Layer Chromatography
Silicagel 70 F254 Plate Wako 100 sheets ( 5 × 20 cm) 197-08404
Silicagel 70 PF254 Plate Wako 10 sheets (20 × 20 cm) 195-12871
Silicagel 70 FM Plate Wako 100 sheets ( 5 × 20 cm) 194-08394
TLC plates used in analysis listed in this catalog
HPLC columns used in analysis listed in this catalog
for other products, please visit the Wako Online Catalog www.e-reagent.com
20
Cru
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TestProduct Name Wako Cat. No. Chemical Structural Formula
Achyranthes Root 015-22271
unidentified
Crude Drug Pkg. Size
Achyranthis Radix 5 g
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
TLC
Sample: Dissolve the 2 g in 10 mL of water. → Shake for 10 min. → Add 5 mL of 1-Butanol. → Shake for 10 min. → The supernatant after centrifugation.
Injection: 10 μLThin-layer plate: Silicagel plate for TLCDeveloping solvent: Ethyl Acetate 5 + Water 1 + Formic Acid 1 (volume ratio) or
1-Propanol 4 + Ethyl Acetate 4 + Water 3 (volume ratio)Distance spot travels: abt. 10 cmDetection method: Dry at RT. → Spray diluted sulfuric acid.→Dry at 105℃ for 5 min.
→ Visual judgment.
Product Name Wako Cat. No. Chemical Structural Formula
Aconitine Standard, 99.0+ % (HPLC) 019-14621
C34H47NO11 = 645.74CAS No. 302-27-2
Crude Drug Pkg. Size
Aconiti Rhizoma 20 mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
TLC HPLC
Sample: 2 mg/mL (Methanol)Sample spotted: 10 μLThin-layer plate: Silicagel 70FM Plate-Wako, 20 × 5 cm [Wako Cat. No.194-08394]Developing solvent: The upper layer of [Butyl Acetate 8 + 1-Propanol1 + Ammonia
Solution 1 (volume ratio)]Distance spot travels: abt. 10 cmDetection method: ① Spray Dragendorff's Reagent 1) →Visual judgment.
②UV exposure
1) Bismuth (III) Nitrate Basic 0.17 g + Acetic Acid 2 mL + Water 8 mL → Shake → Add Potassium Iodide Solution (400 g/L) 10 mL → Shake
Sample: 1 mg/mL (Acetonitrile)Injection: 10 μLColumn: Wakosil-II 5C18 AR, 4.6 mm φ × 15 cm
[Wako Cat. No.: 234-50753 (W), 238-50751 (D)] *Column Temperature: Room temperatureEluent: 0.05 mol/L Phosphate buffer 1) 68 + Acetonitrile 32 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 254 nm
1) [Disodium Hydrogen Phosphate 7.1 g + Water (→1 L)] + [Potassium Dihydrogen Phosphate 6.8 g + Water (→1 L)] (→ pH 7.4).
Product Name Wako Cat. No. Chemical Structural Formula
Acteoside Standard 【Verbascoside】, 98.0+ % (HPLC)
013-24531
C29H36O15 = 624.59CAS No. 61276-17-3
Crude Drug Pkg. Size
Cistanche Herb 10 mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
TLC HPLC
Sample: 10 mg/2 mL (Methanol) Sample spotted: 20 μLThin-layer plate: Silicagel 70 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08384]Developing solvent: Ethyl Acetate 20 + Methanol 3 + Water 2 (volume ratio)Distance spot travels: abt. 7 cmDetection method: Dry at RT. → Spray 2,6-Dibromo-N-Chloro-p-benzoquinone
Monoimine Solution 1). →Leave it in ammonia gas.→ Visual judgment.
1) 2,6-Dibromo-N-Chloro-p-benzoquinone Monoimine 0.5 g + Methanol→100 mL
Sample: 2 mg/10 mL (Eluent)Injection: 10 μLColumn: Wakosil-II 5C18HG,4.6 mm φ × 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 40℃Eluent: 0.1 vol% Phosphoric Acid 85 + Acetonitrile 15 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 330 nm
* (W) and (D) show joint type: (W): Waters type; (D): DuPont type)
21
Cru
de
Dru
g Te
stProduct Name Wako Cat. No. Chemical Structural Formula
Alisol B Acetate, 98.0+ % (HPLC) 018-13231
C32H50O5 = 514.74CAS No. 26575-95-1
Crude Drug Pkg. Size
Alismatis Rhizoma 20 mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
TLC HPLC
Sample: 1 mg/mL (Methanol)Sample spotted: 10 μLThin-layer plate: Silicagel 70 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08384]Developing solvent: Toluene 55 + Acetone 35 + Hexane 10 (volume ratio)Distance spot travels: abt. 10 cmDetection method: Dry at RT. → Dry at 100℃ for 10 min. →Allow to cool
→Spray Sulfuric Acid‐Ethanol (99.5) solution (1+20) → Dry at RT → Heat at 200℃ for 10 min. → Visual judgment.
Sample: 0.2 mg/mL (Eluent)Injection: 10 μLColumn: Wakosil 5C18,4.6 mm φ × 15 cm
[Wako Cat. No.: 239-53983 (W), 233-53981 (D)] *Column Temperature: Room temperatureEluent: Acetonitrile 80 + Water 20 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 203 nm
Product Name Wako Cat. No. Chemical Structural Formula
Alisol B Standard, 99.0+ % (HPLC) 010-13431
C30H48O4 = 472.70CAS No. 18649-93-9
Crude Drug Pkg. Size
Alismatis Rhizoma 20 mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
TLC HPLC
Sample: 1 mg/mL (Methanol) Sample spotted: 10 μLThin-layer plate: Silicagel 70 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08384]Developing solvent: Toluene 55 + Acetone 35 + Hexane 10 (volume ratio)Distance spot travels: abt. 10 cmDetection method: Spray sulfuric acid (10 vol%)→Heat at 120℃ for 5 min.
Sample: 0.2 mg/mL (Eluent)Injection: 10 μLColumn: Wakosil 5C18,4.6 mmφ x 15 cm
[Wako Cat. No.: 239-53983 (W), 233-53981 (D)] *Column temperature: Room TemperatureEluent: Acetonitrile 75 + Water 25 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 203nm
Product Name Wako Cat. No. Chemical Structural Formula
Alkannin Standard, 99.0+ % (HPLC) 013-19901
C16H16O5 = 288.30CAS No. 517-88-4
Crude Drug Pkg. Size
Lithospermum erythrorhizon 10 mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
TLC HPLC
Sample: 5 mg/mL (Acetone) Sample spotted: 5 μLThin-layer plate: Silicagel 70 F254 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08404]Developing solvent: Ethyl Acetate 3 + Ethanol (95) 1 (volume ratio)Distance spot travels: abt.10 cmDetection method: UV exposure and Visual judgment
Sample: 1 mg/mL (Methanol)Injection: 5 μLColumn: Wakosil-II 5C18 HG, 4.6 mmφ × 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 40℃Eluent: 2 vol% Phosphoric Acid solution 25 + Methanol 75(volume ratio)Flow rate: 1.0 mL/minDetector: UV 273nm
for other products, please visit the Wako Online Catalog www.e-reagent.com
22
Cru
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TestProduct Name Wako Cat. No. Chemical Structural Formula
Arctiin Standard, 98.0+% (HPLC) 019-24631
C27H34O11 = 534.55CAS No. 20362-31-6
Crude Drug Pkg. Size
Arctium lappa L. 20 mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
HPLC
Sample: 5 mg/5 mL (Eluent)Injection: 10μlColumn: Wakosil-II 5C18HG, 4.6 mmφ×15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 40℃Eluent: Water 75 + Acetonitrile 25 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 278nm
Product Name Wako Cat. No. Chemical Structural Formula
Artemisia Argyi 018-24221
Unidentified
Crude Drug Pkg. Size
Artemisiae Folium 10 g
Grade Storage
for the JP Crude Drugs Test (for Purity Determination)
Keep at 2-10℃.
Example analysis
TLC
Sample: Add the 0.5 g in 5 mL of [Methanol 3 + Water 2 (volume ratio)]. → Shake for 10 min. → Centrifuged supernatant.
Injection: 10 μLThin-layer plate: Octadecylsilanized silica gel for TLCDeveloping solvent: Methanol 4 + Water 1 (volume ratio) Distance spot travels: abt. 7 cmDetection method: Dry at RT. →Spray diluted sulfuric acid.→Dry at 105℃ for 5
min. →UV exposure (dominant wavelength: 365 nm). → Green fluorescing spots at around 0.3 and 0.4 of Rf value are confirmed as identification of eupatilin and jaceosidin.
Product Name Wako Cat. No. Chemical Structural Formula
Atractylodin Standard, 98.0+ % (HPLC) 011-21531
C13H10O = 182.22CAS No. 55290-63-6
Crude Drug Pkg. Size
Atractylodis Lanceae 20 mg
Grade Storage
for Crude Drugs Determination Keep at -80℃.
Example analysis
TLC HPLC
Sample: 20 μg/mL (Hexane)Sample spotted: 5 μLThin-layer plate: Silicagel 70 F254 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08404]Developing solvent: Hexane 7 + Acetone 1 (volume ratio) Distance spot travels: abt. 10 cmDetection method: Dry at RT. →Spray p-dimethylaminobenzaldehyde solution.
→ Heat at 105℃ for 5 min. →Visual judgment
Sample: 20 μg/mL (Methanol)Injection: 20 μLColumn: Wakosil-II 5C18 RS, 4.6 mmφ × 15 cm
[Wako Cat. No.: 234-51473 (W), 238-51471 (D)] *Column Temperature: 40℃Eluent: [Water 55 + Phosphoric Acid 1 (volume ratio)] 33 + Acetonitrile 67 (volume ratio)Flow rate: 0.8 mL/min Detector: UV 336 nm
* (W) and (D) show joint type: (W): Waters type; (D): DuPont type)
23
Cru
de
Dru
g Te
stProduct Name Wako Cat. No. Chemical Structural Formula
Aucubin Standard, 97.0+ % (HPLC) 016-10351
C15H22O9 = 346.33CAS No. 479-98-1
Crude Drug Pkg. Size
Plantaginis Semen 20 mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
TLC HPLC
Sample: 5 mg/mL (Methanol)Sample spotted: 5 μLThin-layer plate: Silicagel 70 FM Plate-Wako, 20 × 5 cm [Wako Cat. No.194-08394]Developing solvent: The lower layer of [Chloroform 26 + Methanol 14
+ Water 5 (volume ratio)]Distance spot travels: abt. 10 cmDetection method: Spray [p‐Anisaldehyde 1g + Ethanol (99.5) 50 mL
+ Sulfuric Acid (1+5) 50 mL] → Heat → Visual judgment
Sample: 1 mg/mL (Eluent)Injection: 10 μLColumn: Wakosil 5C18, 4.6 mm φ × 15 cm
[Wako Cat. No.: 239-53983 (W), 233-53981 (D)] *Column Temperature: Room TemperatureEluent: Acetonitrile 3 + Water 97 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 206 nm
Product Name Wako Cat. No. Chemical Structural Formula
Baicalein Standard, 98.0+ % (HPLC) 027-07751
C15H10O5 = 270.24CAS No. 491-67-8
Crude Drug Pkg. Size
Scutellariae Radix 20 mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
TLC HPLC
Sample: 1 mg/mL (Methanol)Sample spotted: 2 μLThin-layer plate: Silicagel 70 FM Plate-Wako, 20 × 5 cm [Wako Cat. No.194-08394]Developing solvent: Acetic Acid 10 + Ethyl Acetate 70 + Methanol 30
+ Water 5 (volume ratio) Distance spot travels: abt. 10 cmDetection method: UV exposure (dominant wavelength: 254 nm)
Sample: 0.1 mg/mL (Methanol)Injection: 10 μLColumn: Wakosil-II 5C18, 4.6 mmφ × 15 cm
[Wako Cat. No.: 236-51813 (W), 230-51811 (D)] *Column temperature: a constant temperature aroung room temperature Eluent: 0.1 mol/L Phosphate buffer (pH2.1) 1) 40 + Methanol 60 (volume ratio)Flow rate: 1.0 mL/min Detector: UV 280 nm1) Potassium Dihydrogen Phosphate 2.72 g + Water 180 mL → Adjust the pH to 2.1 with Phosphoric Acid. + Water (→ 200 mL).
Product Name Wako Cat. No. Chemical Structural Formula
Bufotalin Standard, 99.0+ % (HPLC) 024-10691
C26H36O6 = 444.57CAS No. 471-95-4
Crude Drug Pkg. Size
Bufo bufo gargarizans Cantor 20 mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
TLC HPLC
Sample: 1 mg/mL (Methanol)Sample spotted: 10 μLThin-layer plate: Silicagel 70 FM Plate-Wako, 20 × 5 cm [Wako Cat. No.194-08394]Developing solvent: Acetone 3 + Chloroform 3 + Cyclohexane 4 (volume ratio)Distance spot travels: abt. 10 cmDetection method: Spray Cerium Sulfate (IV)・Sulfuric Acid (1+5) Solution (10 g/L)
→ Heat at 105℃ for 5 min.
Sample: 0.5 mg/mL (Eluent)Injection: 10 μLColumn: Wakosil 5C18, 4.6 mmφ × 15 cm
[Wako Cat. No.: 239-53983 (W), 233-53981 (D)] *Column Temperature: Room temperatureEluent: Acetonitrile 7 + Water 13 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 280 nm
for other products, please visit the Wako Online Catalog www.e-reagent.com
24
Cru
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TestProduct Name Wako Cat. No. Chemical Structural Formula
Calycosin 7-O-Glucoside Standard, 98.0+ % (HPLC)
039-22271
C22H22O10 = 446.4CAS No. 20633-67-4
Crude Drug Pkg. Size
Astragali Radix 10 mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
HPLC
Sample: 0.2 mg/mL (Methanol)Injection: 10 μLColumn: Wakosil-II 5C18 RS, 4.6 mmφ × 15 cm
[Wako Cat. No.: 234-51473 (W), 238-51471 (D)] *Column Temperature: 40℃ Eluent: Water 850 + Acetonitrile 150 + Acetic Acid 1 (volume ratio) Flow rate: 1.0 mL/min Detector: UV 254 nm
Product Name Wako Cat. No. Chemical Structural Formula
Carbazochrome Sodium Sulfonate Trihydrate, 99.0+ % (Calculated on the dehydrous basis)
039-21551
C10H11N4NaO5S・3H2O = 376.32CAS No. 51460-26-5
Crude Drug Pkg. Size
Gardeniae Fructus 500 mg
Grade Storage
for Crude Drugs Determination Keep at RT.
Example analysis
HPLC
Sample: 0.5 mg/mL (Water)Injection: 10 μLColumn: ODS (particle size: 7 μm), 4.6 mmφ × 25 cm Column Temperature: 40℃ Eluent: 10 mmol/L Phosphate buffer 1) 925 + Ethanol (95) 75 (volume ratio)
+ Phosphoric Acid (→pH 3.0)Detector: UV 360 nm
1) Ammonium dihydrogen phosphate 1.2 g + Water 1,000 mL
Product Name Wako Cat. No. Chemical Structural Formula
Catalpol Standard, 98.0+ % (HPLC) 032-10551
C15H22O10 = 362.33CAS No. 2415-24-9
Crude Drug Pkg. Size
Rehmanniae Radix 20 mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
TLC HPLC
Sample: 5 mg/mL (Methanol)Sample spotted: 10 μLThin-layer plate: Silicagel 70 FM Plate-Wako, 20 x 5 cm [Wako Cat. No.194-08394]Developing solvent: The lower layer of [Chloroform 65 + Methanol 35
+ Water 10 (volume ratio)]Distance spot travels: abt. 10 cmDetection method: Spray Sulfuric Acid (1+10). →Heat →Visual judgment
Sample: 0.5 mg/mL (Eluent)Injection:10 μLColumn: Wakosil 5C18, 4.6 mmφ x 15 cm
[Wako Cat. No.: 239-53983 (W), 233-53981 (D)] *Column Temperature: 35℃Eluent: Acetonitrile 1 + Water 99 (volume ratio) Flow rate: 1.0 mL/minDetector: UV 203 nm
* (W) and (D) show joint type: (W): Waters type; (D): DuPont type)
25
Cru
de
Dru
g Te
stProduct Name Wako Cat. No. Chemical Structural Formula
Cinobufotalin Standard, 98.0+ % (HPLC) 035-13721
C26H34O7 = 458.55CAS No. 1108-68-5
Crude Drug Pkg. Size
Bufo bufo gargarizans Cantor 20 mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
TLC HPLC
Sample: 1 mg/mL (Methanol)Sample spotted: 10 μLThin-layer plate: Silicagel 70 FM Plate-Wako, 20 × 5 cm [Wako Cat. No.194-08394]Developing solvent: Acetone 3 +Chloroform 3 +Cyclohexane 4 (volume ratio)Distance spot travels: abt. 10 cmDetection method: Spray Cerium Sulfate (IV)・Sulfric Acid (1+5) Solution (1 w/v%)
→ Heat at 105℃ for 5 min.→Visual judgment.
Sample: 1 mg/mL (Eluent)Injection: 10 μLColumn: Wakosil 5C18, 4.6 mmφ × 15 cm
[Wako Cat. No.: 239-53983 (W), 233-53981 (D)] *Column Temperature: Room TemperatureEluent: Acetonitrile 40 + Water 60 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 270 nm
Product Name Wako Cat. No. Chemical Structural Formula
Corosolic Acid Standard, 98.0+ % (HPLC) 030-20741
C30H48O4 = 472.70CAS No. 4547-24-4
Crude Drug Pkg. Size
Lagerstroemia speciosa 10 mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
TLC HPLC
Sample: 1 mg/mL (Methanol)Sample spotted: 5 μLThin-layer plate: Silicagel 70 Plate-Wako, 20 × 5 cm [Wako Cat. No.197-08384]Developing solvent: Chloroform 10 + Methanol 1 (volume ratio)Distance spot travels: abt. 10 cmDetection method: Dry at RT. → Spray Sulfuric Acid-Ethanol (99.5) solution (1+20).
→Dry at RT.→Heat at 130℃ for 10 min.→Visual judgment
Sample: 1 mg/mL (Methanol)Injection: 20 μLColumn: Wakosil-II 5C18 HG, 4.6 mmφ × 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 40℃Eluent: 0.1 vol% Trifluoroacetic Acid 35 + Acetonitrile 65 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 210nm
Product Name Wako Cat. No. Chemical Structural Formula
Corydaline Standard, 99.0+ % (HPLC) 036-14971
C22H27NO4 = 369.45CAS No. 518-69-4
Crude Drug Pkg. Size
Corydalis Tuber 20 mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
TLC HPLC
Sample: 1 mg/mL (Methanol)Sample spotted: 10 μLThin-layer plate: Silicagel 70 FM Plate-Wako, 20 x 5 cm [Wako Cat. No.194-08394]Developing solvent:Acetone 3 +Chloroform 3 +Cyclohexane 4(volume ratio)Distance spot travels: abt. 10 cmDetection method: Spray Cerium Sulfate (IV)・Sulfuric Acid (1+5) Solution (1 w/v%)
→ Heat at 105℃ for 5 min. →Visual judgment
Sample: 1 mg/mL (Eluent)Injection: 10 μLColumn: Wakosil 5C18, 4.6 mmφ × 15 cm
[Wako Cat. No.: 239-53983 (W), 233-53981 (D)] *Column Temperature: Room TemperatureEluent: Acetonitrile 40 + Water 60 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 270 nm
for other products, please visit the Wako Online Catalog www.e-reagent.com
26
Cru
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rug
TestProduct Name Wako Cat. No. Chemical Structural Formula
Dehydrocostus Lactone Standard, 98.0+ % (HPLC)
042-32711
C15H18O2 = 230.3CAS No. 477-43-0
Crude Drug Pkg. Size
Saussureae Radix 20mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
TLC HPLC
Sample: 2 mg/ 2 mL (Methanol) Sample spotted: 10 μLThin-layer plate: Silicagel 70 FM Plate-Wako, 20 x 5 cm [Wako Cat. No.194-08394] Developing solvent: Acetone 1 + Ethyl Acetate 2 + Hexane 7 (volume ratio) Distance spot travels: abt. 10 cmDetection method: Spray Cerium Sulfate (IV)・Sulfuric Acid (1+5) Solution (10 g/L)
→Heat at 105℃ for 5 min.→Visual judgment
Sample: 0.2 mg/mL (Eluent) Injection:10 μL Column: Wakosil-Ⅱ 5C18HG, 4.6 mmφ×15 cm,
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 30℃Eluent: Acetonitrile 60 + Water 40 (volume ratio)Flow rate: 1.0 mL/min Detector: UV 215nm
Product Name Wako Cat. No. Chemical Structural Formula
Dihydrocapsaicin Standard, 99.0+ % (HPLC)
045-28911
C18H29NO3 = 307.43CAS No. 19408-84-5
Crude Drug Pkg. Size
Capsici Fructus 20 mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
TLC HPLC
Sample: 10 mg/mL (Methanol) Sample spotted: 10 μLThin-layer plate: Silicagel 70 Plate-Wako, 20 × 5 cm [Wako Cat. No.197-08384]Developing solvent: Diethyl Ether 19 + Methanol 1 (volume ratio) Distance spot travels: abt. 12 cmDetection method: Spray 2,6-Dibromo-N-Chloro-p-benzoquinone Monoimine
Solution 1). →Leave it in ammonia gas.→ Visual judgment.
1) 2,6-Dibromo-N-Chloro-p-benzoquinone Monoimine 0.1 g + Methanol 20 mL.
Sample: 0.2 mg/mL (Methanol)Injection: 20 μL Column: Wakosil 5Ph,4.6 mmφ × 25 cm
[Wako Cat. No.: 236-57413 (W), 230-57411 (D)] *Column Temperature: 40℃ Eluent: 0.1 vol% Phosphoric Acid 50 + Acetonitrile 50 (volume ratio)Flow rate: 1.0 mL/min Detector: UV 281 nm
Product Name Wako Cat. No. Chemical Structural Formula
5,7-Dimethoxyflavone Standard, 98.0+ % (HPLC)
040-32991
C17H14O4 = 282.29CAS No. 21392-57-4
Crude Drug Pkg. Size
Kaempferia parviflora, 20 mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
HPLC
Sample: 0.1 mg/mL (Eluent)Injection: 10 μLColumn: Wakosil-II 5C18 HG, 4.6 mmφ × 25 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 40℃ Eluent: Acetonitrile 55 + Water 45 (volume ratio) Flow rate: 1.0 mL/min Detector: UV 260 nm
* (W) and (D) show joint type: (W): Waters type; (D): DuPont type)
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stProduct Name Wako Cat. No. Chemical Structural Formula
2,6-Dimethoxyphenol Standard 047-30681
(CH3O)2C6H3OH = 154.16CAS No. 91-10-1
Crude Drug Pkg. Size
- 5 g
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
Product Name Wako Cat. No. Chemical Structural Formula
Dimethylesculetin Standard 042-32331
C11H10O4 = 206.19CAS No. 120-08-1
Crude Drug Pkg. Size
Artemisiae Capillari Flos 20 mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
TLC HPLC
Sample: 1 mg/mL (Methanol)Sample spotted: 5 μLThin-layer plate: Silicagel 70 FM Plate-Wako, 20 x 5 cm [Wako Cat. No.194-08394] Developing solvent: Hexane 50 + Acetone 50 (volume ratio) Distance spot travels: abt. 10 cm Detection method: UV exposure (Dominant wavelength: 365 nm)
Sample: 0.1 mg/mL (Acetonitrile)Injection: 10 μLColumn: Wakosil-II 5C18 HG, 4.6 mmφ × 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 50℃ Eluent: Water 80 + Acetonitrile 20 (volume ratio) Flow rate: 1.0 mL/min Detector: UV 230 nm
Product Name Wako Cat. No. Chemical Structural Formula
Echinacoside Standard, 98.0+ % (HPLC) 056-08601
C35H46O20 = 786.73CAS No. 82854-37-3
Crude Drug Pkg. Size
Cistanche Herb 10 mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
HPLC
Sample: 5 mg/ 20 mL (Eluent)Injection: 10 μLColumn: Wakosil-II 5C18 HG,4.6 mm φ × 25 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 40℃Eluent: 0.1 vol% Phosphoric acid 85 + Acetonitrile 15 (volume ratio) Flow rate: 1.0 mL/minDetector: UV 330 nm
for other products, please visit the Wako Online Catalog www.e-reagent.com
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TestProduct Name Wako Cat. No. Chemical Structural Formula
Emetine Dihydrochloride Standard, 98.0+ % (HPLC)
059-08211
C29H40N2O4・2HCl = 553.56CAS No. 316-42-7
Crude Drug Pkg. Size
Carapichea ipecacuanha 20mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
HPLC
Sample: 10mg/10ml (Eluent) Injection: 10 μLColumn: Wakosil-II 5C18HG, 4.6 mmφ×15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 50℃Eluent: Sodium 1-heptanesulfonate Solution 1) 50 + Methanol 50(volume ratio) Flow rate: 1.0 mL/minDetector: UV 283nm
1) Sodium 1-heptanesulfonate 2.0g+Water 500mL+Acetic Acid (→pH4.0).
Product Name Wako Cat. No. Chemical Structural Formula
Epihesperidin Standard, 99.0+ % (HPLC) 085-08211
C28H34O15 = 610.56CAS No. 369593-42-0
Crude Drug Pkg. Size
Aurantii Nobilis Pericarpium 10 mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
TLC HPLC
Sample: 125 μg/mL (Methanol) Sample spotted: 10 μLThin-layer plate: Silicagel 70 F254 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08404]Developing solvent: Ethyl Acetate 8 + Ethanol (99.5) 2 + Water 1 (volume ratio)Distance spot travels: abt. 10 cmDetection method: UV exposure (254 nm)
Sample: 125 μg/mL (Methanol)Injection: 10 μLColumn: Wakosil-II 5C18,4.6 mm φ × 15 cm
[Wako Cat. No.: 236-51813 (W), 230-51811 (D)] *Column Temperature: 50℃Eluent: Water 82 + Acetonitrile 18 + Acetic Acid 1 (volume ratio) Flow rate: 0.7 mL/minDetector: UV 285 nm
Product Name Wako Cat. No. Chemical Structural Formula
Ergosterol Standard, 98.0+ % (HPLC) 052-04921
C28H44O = 396.65CAS No. 57-87-4
Crude Drug Pkg. Size
Polyporus 20 mg
Grade Storage
for Crude Drugs Determination Keep at -20℃.
Example analysis
TLC HPLC
Sample: 5 mg/mL (Chloroform)Sample spotted: 5 μLThin-layer plate: Silicagel 70FM Plate-Wako, 20 × 5 cm [Wako Cat. No.194-08394]Developing solvent: Acetone 1 + Chloroform 1 + Toluene 10 (volume ratio)Distance spot travels: abt. 10 cmDetection method: UV exposure
Sample: 0.4 mg/mL (Chloroform 1 + Methanol 1 (volume ratio))Injection: 10 μLColumn: Wakosil 5C18, 4.6 mm φ × 15 cm
[Wako Cat. No.: 239-53983 (W), 233-53981 (D)] *Column Temperature: RTEluent: MethanolFlow rate: 1.0 mL/minDetector: UV 254 nm
* (W) and (D) show joint type: (W): Waters type; (D): DuPont type)
29
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stProduct Name Wako Cat. No. Chemical Structural Formula
β-Eudesmol Standard, 98.0+ % (HPLC) 056-05161
C15H26O = 222.37CAS No. 473-15-4
Crude Drug Pkg. Size
Atractylodis Lanceae 20 mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
TLC HPLC
Sample: 1 mg/mL (Methanol)Sample spotted: 10 μLThin-layer plate: Silicagel 70 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08384]Developing solvent: Acetone 1 + Ethyl Acetate 2 + Hexane 7 (volume ratio)Distance spot travels: abt. 10 cmDetection method: Spray Cerium Sulfate (IV)-Sulfuric Acid (1+5) Solution (10 g/L).
→Heat at 105℃ for 5 min.
Sample: 0.5 mg/mL (Eluent)Injection: 10 μL Column: Navi C30-5, 4.6 mm φ × 15 cm
[Wako Cat. No.: 233-61193 (W), 237-61191 (D)] *Column Temperature: 40℃Eluent: Acetonitrile 60 + Water 40 (volume ratio)Detector: UV 203 nm
Product Name Wako Cat. No. Chemical Structural Formula
Evodiamine Standard, 98.0+ % (HPLC) 051-08411
C19H17N3O = 303.36
Crude Drug Pkg. Size
Evodiae Fructus 20 mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
TLC HPLC
Sample: 1 mg/mL (Chloroform)Injection: 5 μLThin-layer plate: Silicagel 70 F254 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08404]Developing solvent: Hexane 50 + Ethyl Acetate 50 (volume ratio) Distance spot travels: abt. 10 cm Detection method: UV exposure (dominant wavelength: 254 nm)
Sample: 0.2 mg/mL (Acetonitrile) Injection: 10 μLColumn: Wakosil-II 5C18 HG, 4.6 mmφ × 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 40℃ Eluent: Acetonitrile 50 + 0.01 vol% Phosphoric Acid 50 (volume ratio)Flow rate: 1.0 mL/min Detector: UV 270 nm
Product Name Wako Cat. No. Chemical Structural Formula
Geniposidic Acid Standard, 98.0+ % (HPLC)
078-05841
C16H22O10 = 374.34CAS No. 27741-01-1
Crude Drug Pkg. Size
・Gardeniae Fructus・Plantaginis Semen
20 mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
TLC HPLC
Sample: 5 mg/mL (Methanol) Sample spotted: 5 μLThin-layer plate: Silicagel 70 FM Plate-Wako, 20 x 5 cm [Wako Cat. No.194-08394]Developing solvent: Chloroform 13 + Methanol 7 + Water 1 (volume ratio)Distance spot travels: abt. 10 cm Detection method: ①UV exposure at 254 nm
②Spray [Vanillin 1 g + Ethanol (99.5) 50 mL + Sulfuric Acid(1+5) 50 mL]→ Heat at 105℃) →Visual judgment.
Sample: 0.2 mg/mL (Eluent)Sample spotted: 10 μLColumn: Wakosil 5C18, 4.6 mmφ × 15 cm
[Wako Cat. No.: 239-53983 (W), 233-53981 (D)] *Column Temperature: 35℃ Eluent: 2 vol% Acetic Acid 95 + Acetonitrile 5 (volume ratio) Flow rate: 1.0 mL/min Detector: UV 240 nm
for other products, please visit the Wako Online Catalog www.e-reagent.com
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TestProduct Name Wako Cat. No. Chemical Structural Formula
[6]-Gingerol Standard, 98.0+ % (HPLC) 071-06051
C17H26O4 = 294.39
Crude Drug Pkg. Size
Zingiberis Rhizoma 20 mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
TLC HPLC
Sample: 0.5 mg/mL (Methanol) Sample spotted: 10 μLThin-layer plate: Silicagel 70 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08384]Developing solvent: Ethyl Acetate 1 + Hexane 1 (volume ratio) Distance spot travels: abt. 10 cm Detection method: Dry at RT.→ Spray 4-dimethylaminobenzaldehyde Solution 1).
→Heat at 105℃ for 5 min. →Allow to cool. → Visual judgment.
1) 4-dimethylaminobenzaldehyde 10g + chilled [Sulfuric Acid 90 mL + Water 10 mL]
Sample: 1 mg/mL (Methanol)Injection: 10 μLColumn: Wakosil-II 5C18 HG, 4.6 mmφ × 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 30℃ Eluent: Water 620 + Acetonitrile 380 + Phosphoric Acid 1 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 282 nm
Product Name Wako Cat. No. Chemical Structural Formula
[8]-Gingerol Standard, 98.0+% (HPLC) 079-06231
C19H30O4 = 322.44CAS No. 23513-08-8
Crude Drug Pkg. Size
Zingiberis Rhizoma 20 mg
Grade Storage
for Crude Drugs Determination Keep at -20℃.
Example analysis
HPLC
Sample: 5 mg/5 mL (Eluent) Injection: 10 μLColumn: Wakosil-II5C18HG, 4.6 mmφ×15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 30℃Eluent: Water 50 + Acetonitrile 50 + Acetonitrile 0.1 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 280nm
Product Name Wako Cat. No. Chemical Structural Formula
[10]-Gingerol Standard, 98.0+% (HPLC) 072-06221
C21H34O4 = 350.49CAS No. 23513-15-7
Crude Drug Pkg. Size
Zingiberis Rhizoma 20 mg
Grade Storage
for Crude Drugs Determination Keep at -20℃.
Example analysis
HPLC
Sample: 5 mg/5 mL (Eluent) Injection: 10 μLColumn: Wakosil-II 5C18HG, 4.6 mmφ×15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 30℃Eluent: Water 40 + Acetonitrile 60 + Acetonitrile 0.1 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 280nm
* (W) and (D) show joint type: (W): Waters type; (D): DuPont type)
31
Cru
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stProduct Name Wako Cat. No. Chemical Structural Formula
Ginsenoside-Rb1 Standard, 98.0+ % (HPLC)
079-02191
C54H92O23 = 1109.29CAS No. 41753-43-9
Crude Drug Pkg. Size
・Ginseng Radix Rubra・Panacis Ginseng Radix
10 mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
TLC HPLC
Sample: 5 mg/mL (Methanol)Sample spotted: 10 μLThin-layer plate: Silicagel 70 Plate-Wako, 20 × 5 cm [Wako Cat. No.197-08384]Developing solvent: The lower layer of [Chloroform 13 + Methanol 7
+ Water 2 (volume ratio)]Distance spot travels: abt. 10 cmDetection method: Spray [p-Anisaldehyde 1 g + Ethanol (99.5) 50 mL + Sulfuric Acid
(1+5) 50 mL] → Heat →Visual judgment.
Sample: 0.5 mg/mLInjection: 20 μLColumn: Wakosil-II 5C18 HG, 4.6 mmφ × 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 40℃Eluent: Acetonitrile 35 + Water 65 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 203 nm
Product Name Wako Cat. No. Chemical Structural Formula
Ginsenoside-Rd Standard, 99.0+ % (HPLC)
072-03301
C48H82O18 = 947.15CAS No. 52705-93-8
Crude Drug Pkg. Size
・Ginseng Radix Rubra・Panacis Ginseng Radix
20 mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
TLC HPLC
Sample: 1 mg/mL (Methanol)Sample spotted: 10 μL Thin-layer plate: Silicagel 70 Plate-Wako, 20 × 5 cm [Wako Cat. No.197-08384]Developing solvent: The lower layer of [Chloroform 13 + Methanol 7
+ Water 2 (volume ratio)]Distance spot travels: abt. 10 cmDetection method: Spray [p-methoxybenzaldehyde 1 g + Ethanol (99.5) 50 mL
+ Sulfuric Acid (1+5) 50 mL] → Heat → Visual judgment.
Sample: 0.5 mg/mL (Eluent) Injection: 20 μLColumn: Wakosil-II 5C18 HG, 4.6 mmφ × 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 40℃Eluent: Acetonitrile 35 + Water 65 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 203 nm
Product Name Wako Cat. No. Chemical Structural Formula
Glabridin, 97.0+ % (HPLC) 070-04821
C20H20O4 = 324.37CAS No. 59870-68-7
Crude Drug Pkg. Size
Glycyrrhizae Radix 20 mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
TLC HPLC
Sample: 1 mg/mL (Ethanol)Injection: 5 μLThin-layer plate: Silicagel 70 F254 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08404] Developing solvent:Hexane 5 + Diethyl Ether 2 + Ethanol (99.5) 2 (volume ratio) Distance spot travels: abt. 10 cm Detection method: UV exposure (254nm)
Sample: 0.2 mg/mL (Eluent)Injection: 20 μLColumn: Wakosil 5C18, 4.6 mmφ × 25 cm
[Wako Cat. No.: 239-53983 (W), 233-53981 (D)] *Column Temperature: 40℃ Eluent: 2 vol% Acetic Acid 40 + Acetonitrile 60 (volume ratio) Flow rate: 1.0 mL/min Detector: UV 282 nm
for other products, please visit the Wako Online Catalog www.e-reagent.com
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TestProduct Name Wako Cat. No. Chemical Structural Formula
Glycocholic Acid Standard, 98.0+ % (HPLC)
072-05361
C26H43NO6 = 465.62CAS No. 475-31-0
Crude Drug Pkg. Size
Fel Ursi 20 mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
TLC HPLC
Sample: 5 mg/mL (Methanol)Sample spotted: 5 μLThin-layer plate: Silicagel 70 Plate-Wako, 20 × 5 cm [Wako Cat. No.197-08384]Developing solvent: Toluene 10 + Acetic Acid 10 + Water 1 (volume ratio)Distance spot travels: abt. 10 cmDetection method: Dry at RT. → Spray Sulfuric Acid (1+18) → Heat at 105℃ for 10 min.
→ Visual judgment
Sample: 0.45 mg/mL (Methanol) Injection: 10 μLColumn: Wakosil-II 5C18 HG, 4.6 mmφ × 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 40℃Eluent: 20 mmol/L Phosphate buffer (pH 3.0) 1) 67 + Acetonitrile 33 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 200 nm
1) Potassium Dihydrogen Phosphate1.36g + Water 450 mL + Phosphoric Acid (→pH 3.0) + Water (→500 mL)
Product Name Wako Cat. No. Chemical Structural Formula
Glycohyodeoxycholic Acid Standard, 98.0+ % (HPLC)
073-06251
C26H43NO5 = 449.62CAS No. 13042-33-6
Crude Drug Pkg. Size
・Powdered Swine Bile・Fel Ursi
50mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
TLC HPLC
Sample: 10 mg/5 mL (Methanol)Sample spotted: 2 μLThin-layer plate: Silicagel 60 Plate, 20 x 5 cm Developing solvent: Ethyl Acetate 12 + Methanol 2 + Formic Acid 1 (volume ratio) Distance spot travels: abt. 7 cm Detection method: Dry at RT. →Spray [Vanillin 3 g + Ethanol (99.5) 100 mL + Sulfuric
Acid 0.5 mL]→ Heat at 105℃ for 10 min.→Visual judgment.
Sample: 5 mg/ 5 mL (Methanol) Injection: 10 μLColumn: Wakosil-II 5C18 HG, 4.6 mmφ × 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 40℃Eluent: 20 mmol/L Phosphate buffer (pH 3.0) 1) 64 + Acetonitrile 36 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 200 nm
1) Potassium Dihydrogen Phosphate 1.42g + Water 400 mL + Phosphoric Acid (→pH 3.0) + Water (→500 mL)
Product Name Wako Cat. No. Chemical Structural Formula
Glycyrrhizic Acid Standard, 99.0+ % (HPLC)
071-02271
C42H62O16 = 822.93CAS No. 1405-86-3
Crude Drug Pkg. Size
Glycyrrhizae Radix 20 mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
TLC HPLC
Sample: 2 mg/mL (Ethanol (50 vol%))Sample spotted: 10 μLThin-layer plate: Silicagel 70 FM Plate-Wako, 20 x 5 cm [Wako Cat. No.194-08394] Developing solvent: 1-Butanol 7 + Acetic Acid 1 + Water 2 (volume ratio) Distance spot travels: abt. 10 cm Detection method: UV exposure (254 nm)
Sample: 0.25 mg/mL (Ethanol (50 vol%))Injection: 20 μLColumn: Wakosil 5C18-200, 4.6 mmφ × 25 cm
[Wako Cat. No.: 237-55503 (W), 231-55501 (D)] *Column Temperature: 40℃ Eluent: Acetic Acid (6.7 vol%) 1) 3 + Acetonitrile 2 (volume ratio) Flow rate: 0.8 mL/min Detector: UV 254 nm
1) Acetic Acid 20 mL + Water (→300 mL).
* (W) and (D) show joint type: (W): Waters type; (D): DuPont type)
33
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stProduct Name Wako Cat. No. Chemical Structural Formula
Gomisin A Standard, 98.0+ % (HPLC) 077-04951
C23H28O7 = 416.46CAS No. 58546-54-6
Crude Drug Pkg. Size
Schisandrae Fructus 10mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
TLC HPLC
Sample: 2.5 mg/mL (Acetonitrile)Sample spotted: 1 μLThin-layer plate: Silicagel 70 F254 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08404]Developing solvent: Ethyl Acetate 10 + Hexane 10 + Acetic Acid 1 (volume ratio)Distance spot travels: abt. 10 cmDetection method: UV 254 nm
Sample: 1 mg/mL (Acetonitrile)Injection: 5 μLColumn: Wakosil-II 5C18 HG,4.6 mmφ × 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 40℃Eluent: Methanol 60 + Water 40 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 254 nm
Product Name Wako Cat. No. Chemical Structural Formula
Gomisin N Standard, 98.0+ % (HPLC) 074-04961
C23H28O6 = 400.46CAS No. 69176-52-9
Crude Drug Pkg. Size
Schisandrae Fructus 10 mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
TLC HPLC
Sample: 2.5 mg/mL (Acetonitrile)Sample spotted: 1 μLThin-layer plate: Silicagel 70 F254 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08404]Developing solvent: Ethyl Acetate 10 + Hexane 10 + Acetic Acid 1 (volume ratio)Distance spot travels: abt. 10 cmDetection method: UV 254 nm
Sample: 1 mg/mL (Acetonitrile)Injection: 5 μLColumn: Wakosil-II 5C18 HG,4.6 mmφ × 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 40℃Eluent: Methanol 75 + Water 25 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 254 nm
Product Name Wako Cat. No. Chemical Structural Formula
Hypaconitine, 98.0+ % (HPLC) 084-07061
C33H45NO10 = 615.72CAS No. 6900-87-4
Crude Drug Pkg. Size
Aconiti Rhizoma 20mg
Grade Storage
for Crude Drugs Determination Keep below 25℃.
Example analysis
TLC HPLC
Sample: 5 mg/ 5mL (Methanol) Injection: 20 μLThin-layer plate: Silicagel 70FM Plate-Wako, 20 × 5 cm [Wako Cat. No.194-08394]Developing solvent: 1-Propanol 7 + Ammonia Solution 3 (volume ratio)Distance spot travels: abt. 10 cmDetection method: UV exposure
Sample: 1 mg/mL (Methanol) Injection: 10 μLColumn: Wakosil-II 5C18 HG, 4.6 mmφ × 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 40℃Eluent: 0.05 mol/L Phosphate buffer (pH 2.4) 1) 55 + Tetrahydrofuran 7
+ Acetonitrile 2 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 254 nm
1) Potassium Dihydrogen Phosphate1.36 g + Water 180 mL →Adjust the pH to 2.4 with Phosphoric Acid + Water (→200 mL).
for other products, please visit the Wako Online Catalog www.e-reagent.com
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TestProduct Name Wako Cat. No. Chemical Structural Formula
Isoacteoside Standard, 98.0+ %(HPLC) 095-06671
C29H36O15 = 624.59CAS No. 61303-13-7
Crude Drug Pkg. Size
Cistanche Herb 10mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
TLC HPLC
Sample: 10 mg/2 mL (Methanol)Sample spotted: 20 μLThin-layer plate: Silicagel 70 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08384]Developing solvent: Ethyl Acetate 20 + Methanol 3 + Water 2 (volume ratio)Distance spot travels: abt. 7 cmDetection method: Dry at RT. → Spray 2,6-Dibromo-N-Chloro-p-benzoquinone
Monoimine Solution 1). →Leave it in ammonia gas. → Visual judgment.
1) 2,6-Dibromo-N-Chloro-p-benzoquinone Monoimine 0.5 g + Methanol (→100 mL)
Sample: 2 mg/10 mL (Eluent)Injection: 10 μLColumn: Wakosil-II 5C18HG,4.6 mm φ × 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 40℃Eluent: 0.1 vol% Phosphoric Acid 85 + Acetonitrile 15 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 330 nm
Product Name Wako Cat. No. Chemical Structural Formula
Isofraxidine Standard, 99.0+ % (HPLC) 099-03651
C11H10O5 = 222.19CAS No. 486-21-5
Crude Drug Pkg. Size
Acanthopanax Root Bark 20 mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
TLC HPLC
Sample: 1 mg/mL (Methanol)Sample spotted: 2 μLThin-layer plate: Silicagel 70 FM Plate-Wako, 20 x 5 cm [Wako Cat. No.194-08394]Developing solvent: Acetic Acid 1 + 1-Butanol 5 + Water 2 (volume ratio) Distance spot travels: abt. 10 cm Detection: UV exposure
Sample: 0.2 mg/mL (Methanol)Injection: 5 μLColumn: Wakosil 5C18, 4.6 mm φ × 15 cm
[Wako Cat. No.: 239-53983 (W), 233-53981 (D)] *Column Temperature: 40℃ Eluent: 0.1% Acetic Acid 80 + Acetonitrile 20 (volume ratio) Flow rate: 1.0 mL/min Detector: UV 354 nm
Product Name Wako Cat. No. Chemical Structural Formula
(Z)-Ligustilide Standard Solution (0.1 mg/mL Methanol Solution)
121-05801
C12H14O2 = 190.24CAS No. 4431-01-0
Crude Drug Pkg. Size
Angelicae Radix 500 μL x 5A
Grade Storage
for Crude Drugs Determination Keep at -20℃.
Example analysis
TLC HPLC
Sample spotted: 1 μLThin-layer plate: Silicagel 70 F254 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08404]Developing solvent: Ethyl Acetate 1 + Hexane 1 (volume ratio)Distance spot travels: abt. 10 cm Detection: UV exposure
Injection: 20 μLColumn: Wakosil-II 5C18 RS, 4.6 mm φ × 15 cm
[Wako Cat. No.: 234-51473 (W), 238-51471 (D)] *Column Temperature: 40℃Eluent: Phosphoric Acid (1+55) 40 + Acetonitrile 60 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 326 nm
* (W) and (D) show joint type: (W): Waters type; (D): DuPont type)
35
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stProduct Name Wako Cat. No. Chemical Structural Formula
Magnoflorine Standard, 95.0+% (HPLC) 138-17201
C20H24NO4 = 342.41CAS No. 2141-09-5
Crude Drug Pkg. Size
Magnoliae Flos 5 mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
TLC HPLC
Sample: 1 mg/mL (Methanol)Sample spotted: 5 μLThin-layer plate: Silicagel 70 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08384]Developing solvent: Ethyl Acetate 5 + Acetone 3 + Water 1 + Formic Acid 1 (volume ratio)Distance spot travels: abt. 10 cmDetection method: Air drying →Spray Dragendorff's Reagent 1) →Visual judgment.
1) Bismuth Nitrate Basic 0.85 g + Acetic Acid 10 mL + Water 40 mL (Dissolve by shaking vigorously)(= Soln. A). Potassium Iodide 8 g + Water 20 mL (= Soln. B). Just before use, mix equal part of Soln. A and Soln. B. Take the 4 mL of the mixture and add 20 mL of 1 mol/L Acetic Acid.
Sample: 0.2 mg/mL (Methanol) Injection: 20 μLColumn: Wakosil-II 5C18 RS, 4.6 mm φ × 15 cm
[Wako Cat. No.: 234-51473 (W), 238-51471 (D)] *Column Temperature: 40℃Eluent: SDS Solution 1) 65 + Acetonitrile 35 + Phosphoric Acid 0.1 (volume ratio) Flow rate: 1.0 mL/minDetector: UV 270 nm 1) Sodium Dodecyl Sulfate 1 g + Water (→130 mL).
Product Name Wako Cat. No. Chemical Structural Formula
Magnoflorine Iodide Standard, 95.0+% (qNMR)
138-17941
C20H24INO4 = 469.31CAS No. 4277-43-4
Crude Drug Pkg. Size
Magnoliae Flos 10mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
TLC HPLCSample: 5 mg + 1 mL of [Methanol 1 + Water 1] → ultrasonic irradiation + 2 mL of
[Methanol 1 + Water 1]Sample spotted: 10 μLThin-layer plate: Silicagel 60, 20 x 5 cm Developing solvent: Ethyl Acetate 5 + Acetone 3 + Water 1 + Formic Acid 1 (volume ratio)Distance spot travels: abt. 7 cmDetection method: Dry at RT.→Spray Dragendorff Solution 1). → Dry at RT.→Spray
Sodium Nitrite Solution 2)→ Visual judgment.
1) Bismuth Subnitrate 0.85g + Acetic Acid 10 mL +Water 40 mL (Beat Up) → [Soln. C]. Potassium Iodide 8 g + Water 20 mL → [Soln. D]. Soln. C 2 mL + Soln. D 2 mL + 1 mol/L Acetic Acid 20 mL (Prepare these solutions just before use.)
2) Sodium Nitrite 10 g + Water (→ 100 mL)
Sample: 5 mg + 5 mL of [Methanol 1 + Water 1] → Ultrasonic Irradiation → Add [Methanol 1 + Water 1] to prepare the 10 mL soln.
Injection: 10 μLColumn: Wakosil-II 5C18 RS, 4.6 mmφ × 15 cm
[Wako Cat. No.: 234-51473 (W), 238-51471 (D)] *Column Temperature: 40℃Eluent: Sodium Lauryl Sulfate 5 g + Acetonitrile 350 mL + Water 650 mL
+ Phosphoric Acid 1 mL (volume ratio) Flow rate: 1.0 mL/minDetector: UV 303 nm
Product Name Wako Cat. No. Chemical Structural Formula
Mesaconitine, 98.0+% (HPLC) 130-12261
C33H45NO11 = 631.71CAS No. 2752-64-9
Crude Drug Pkg. Size
Aconiti Rhizoma 20 mg
Grade Storage
for Crude Drugs Determination Keep below 25℃.
Example analysis
TLC HPLC
Sample: 2 mg/mL (Methanol)Sample spotted: 10 μLThin-layer plate: Silicagel 70FM Plate-Wako, 20 x 5 cm [Wako Cat. No.194-08394]Developing solvent: 1-Propanol 7 + Ammonia Solution 3 (volume ratio)Distance spot travels: abt. 10 cmDetection method : UV exposure
Sample: 1 mg/mL (Methanol)Injection: 10 μLColumn: Wakosil-II 5C18 HG, 4.6 mm φ × 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 40℃Eluent: 0.05 mol/L Phosphate buffer (pH 2.4) 1) 90 + Tetrahydrofuran 10 (volume ratio)Flow rate: 1.2 mL/minDetector: UV 236 nm
1) Potassium Dihydrogen Phosphate1.36 g + Water 180 mL→ Adjust the pH to 2.4 with Phosphoric Acid + Water (→ 200 mL).
for other products, please visit the Wako Online Catalog www.e-reagent.com
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TestProduct Name Wako Cat. No. Chemical Structural Formula
Neohesperidin Standard, 98.0+% (HPLC) 142-09191
C28H34O15 = 610.56CAS No. 13241-33-3
Crude Drug Pkg. Size
immature orange 10mg
Grade Storage
for Crude Drugs Determination Keep at -20℃.
Example analysis
TLC HPLC
Sample: 2 mg / 2 mL (Methanol) Sample spotted: 20 μLThin-layer plate: Silicagel 70 Plate-Wako, 20 × 5 cm [Wako Cat. No.197-08384] Developing solvent: Ethyl Acetate 7 + 1-Propanol 5 + Water 4 + Acetic Acid 1 (volume
ratio)Distance spot travels: abt. 10 cmDetection method: Dry at RT.→Spray 2,6-Dibromo-N-Chloro-1,4-benzoquinone
Monoimine Solution 1). →Leave it in ammonia gas.→ Visual judgment.
1) 2,6-Dibromo-N-Chloro-1,4-benzoquinone Monoimine 0.5g + Methanol (→100 mL).
Sample: 2 mg / 10mL (Methanol)Injection: 10 μLColumn: Wakopak Navi C18-5,4.6 mm φ × 15 cm
[Wako Cat. No.: 237-60513 (W),231-60511 (D)] *Column Temperature: 40℃Eluent: Water 82 + Acetonitrile 18 + Acetic Acid 1 (volume ratio) Flow rate: 1.0 mL/minDetector: UV 285 nm
Product Name Wako Cat. No. Chemical Structural Formula
γ-Oryzanol Standard, 98.0+% (molecular absorptiometric analysis)
156-02831
Mixture of steryl ferulates
CAS No. 11042-64-1
Crude Drug Pkg. Size
Oryzae Semen 20 mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
TLC Molecular absorptiometric analysis
Sample: 2.5 mg/mL (Ethyl Acetate)Sample spotted: 10 μLThin-layer plate: Silicagel 60 F254, 20 × 5 cmDeveloping solvent: Hexane 5 + Acetone 2 (volume ratio)Distance spot travels: abt. 10 cmDetection method: UV 365 nm
Sample: 10μg/mL (Heptane 1))Detection method: Measure the absorption maximum wavelength around 315 nm.
1) for HPLC
Product Name Wako Cat. No. Chemical Structural Formula
Oxymatrine Standard, 98.0+% (HPLC) 150-01511
C15H24N2O2 = 264.37CAS No. 16837-52-8
Crude Drug Pkg. Size
Sophorae Radix 20 mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
TLC HPLC
Sample: 5 mg/mL (Methanol)Sample spotted: 10 μLThin-layer plate: Silicagel 70 F254 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08404]Developing solvent: Chloroform1 + Methanol 1 (volume ratio)Distance spot travels: abt. 10 cmDetection method: Dry at RT.→Dry at 80℃ for 10 min.→Allow to cool.→Spray
Dragendorff's Reagent 1)→Visual judgment.
1) Bismuth Nitrate Basic 0.17 g + Acetic Acid 2 mL + Water 8 mL→ Shake + Potassium Iodide Solution (400 g/L) 10 mL→ Shake.
Sample: 0.2 mg/mL (Eluent)Injection: 10 μLColumn: Wakosil 5C18, 4.6 mmφ × 15 cm
[Wako Cat. No.: 239-53983 (W), 233-53981 (D)] *Column Temperature: 35℃Eluent: [0.02 mol/L Phosphate buffer 1) 65 + Acetonitrile 35 (volume ratio)]400 mL + Sodium Dodecyl Sulfate 0.2 gFlow rate: 1.0 mL/minDetector: UV 220 nm
1) Potassium Dihydrogen Phosphate 0.81g + Water (→300 mL) → Adjust the pH to 2.5 with Phosphoric Acid.
* (W) and (D) show joint type: (W): Waters type; (D): DuPont type)
37
Cru
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Dru
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stProduct Name Wako Cat. No. Chemical Structural Formula
Plantago Seed 166-23751
Unidentified
Crude Drug Pkg. Size
Plantaginis Semen 10 g
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
TLC
Sample: Add the 2 g in 3 mL of Methanol and heat for 3 min. in a water bath. After cooling, centrifuge followed by taking the supernatant.
Sample spotted: 10 μL Thin-layer plate: Silicagel plate for TLCDeveloping solvent: Acetone 10 + Ethyl Acetate 10 + Water 3 + Acetic Acid (100)
1 (volume ratio) or Ethyl Acetate 6 + Water 1 + Formic acid 1 (volume ratio)
Distance spot travels: abt. 10 cmDetection method: After air drying, spray 4-methoxybenzaldehyde ・ Sulfuric Acid
reagent. → Heat at 105℃ for 10 min. → Visual judgment
Product Name Wako Cat. No. Chemical Structural Formula
Praeruptorin A Standard, 98.0+% (HPLC) 168-22231
C21H22O7 = 386.4CAS No. 73069-25-7
Crude Drug Pkg. Size
Peucedani Radix 10 mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
TLC HPLC
Sample: 1 mg/mL (Methanol) Sample spotted: 5 μLThin-layer plate: Silicagel 70 F254 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08404]Developing solvent: Diethyl Ether 3 + Hexane 1 (volume ratio)Distance spot travels : abt. 10 cmDetection method: UV 365 nm
Sample: 1 mg/mL (Methanol) Injection: 5 μLColumn: Wakopak Navi C18-5, 4.6 mmφ × 15 cm
[Wako Cat. No.: 237-60513 (W),231-60511 (D)] *Column Temperature: 40℃Eluent: Water 45 + Acetonitrile 55 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 322 nm
Product Name Wako Cat. No. Chemical Structural Formula
Rhaponticin Standard, 98.0+% (HPLC) 189-02721
C21H24O9 = 420.41CAS No. 155-58-8
Crude Drug Pkg. Size
Rhei Rhizoma 5 mg
Grade Storage
for Crude Drugs Determination Keep at -20℃.
Example analysis
TLC HPLC
Sample: 2 mg/mL (Methanol)Sample spotted: 10 μLThin-layer plate: Silicagel 70 F254 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08404] Devedloping Solvent: Ethyl Formate 10 + 2-Butanone 7 + Formic Acid 1
+ Water 1 (volume ratio) Distance spot travels: abt. 10 cmDetection method: UV exposure (dominant wavelength: 365 nm)
Sample: 0.2 mg/mL (Methanol)Sample spotted: 5 μLColumn: Wakosil-II 5C18 HG, 4.6 mmφ × 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 40℃ Eluent: 0.1 vol% Phosphoric Acid 65 + Methanol 35 (volume ratio) Flow rate: 1.0 mL/min Detector: UV 324 nm
for other products, please visit the Wako Online Catalog www.e-reagent.com
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TestProduct Name Wako Cat. No. Chemical Structural Formula
Rutin Standard, 95.0+% (HPLC) 188-02791
C27H30O16 = 610.52CAS No. 153-18-4
Crude Drug Pkg. Size
Crataegi Fructus 20 mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
TLC HPLC
Sample: 5 mg/mL (Methanol) Sample spotted: 2 μLThin-layer plate: Silicagel 70 FM Plate-Wako, 20 x 5 cm [Wako Cat. No.194-08394]Developing solvent: Ethyl Acetate 5 + 2-Butanone 3 + Water 1 + Formic Acid 1 (volume
ratio)Distance spot travels: abt. 10 cmDetection method: After air drying, spray diluted sulfuric acid.→After heating at 105℃
for 5 min, expose to UV (dominant wavelength: 365 nm)
Sample: 0.4 mg/mL (Methanol) Injection: 5 μLColumn: Wakosil 5C18,4.6 mm φ × 15 cm
[Wako Cat. No.: 239-53983 (W), 233-53981 (D)] *Column Temperature: 40℃Eluent: Water 70 + Methanol 30 + Acetic Acid 5 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 254nm
Product Name Wako Cat. No. Chemical Structural Formula
Saikosaponin c Standard, 99.0+% (HPLC) 197-08421
C48H78O17 = 927.12CAS No. 20736-08-7
Crude Drug Pkg. Size
Bupleuri Radix 10 mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
TLC HPLC
Sample: 1 mg/mL (Methanol) Sample spotted: 10 μLThin-layer plate: Silicagel 70 FM Plate-Wako, 20 x 5 cm [Wako Cat. No.194-08394]Developing solvent: The lower layer of [Chloroform 7 + Methanol 3
+ Water 1 (volume ratio)]Distance spot travels: abt. 10 cmDetection method: Spray [p-Anisaldehyde 1 g + Ethanol (99.5) 50 mL
+ Sulfuric Acid (1+5) 50 mL].→ Heat →Visual judgment.
Sample: 0.5 mg/mL (Eluent)Injection: 10 μLColumn: Wakosil-II 5C18 HG,4.6 mmφ × 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 35℃Eluent: Acetonitrile 35 + Water 65 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 203 nm
Product Name Wako Cat. No. Chemical Structural Formula
Scopolamine Hydrobromide n-Hydrate Standard, 99.0+% (HPLC)
190-16341
C17H21NO4・HBr = 384.26CAS No. 114-49-8
Crude Drug Pkg. Size
Scopoliae Rhizoma 20 mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
TLC HPLC
Sample: 0.6 mg/mL (Ethanol (99.5))Sample spotted: 10 μLThin-layer plate: Silicagel 70 Plate-Wako, 20 × 5 cm [Wako Cat. No.197-08384]Developing solvent: Methanol 200 + 28% Ammonia Solution 3 (volume ratio)Distance spot travels : abt. 10 cmDetection method: Dry at RT.→Spray Dragendorff's Reagent 1) → Visual judgment.
1) Bismuth (III) Nitrate Basic 0.17g + Acetic Acid 2 mL+ Water 8 mL → Shake + Potassium Iodide Soln. (400 g/L) 10 mL→ Shake.
Sample: 1.1 mg/mL (Eluent)Injection: 10 μLColumn: Wakosil-II 5C18 HG, 4.6 mmφ × 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 40℃Eluent: Phosphoric Acid・Triethylamine buffer 1) 9 + Acetonitrile 1 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 210 nm
1) Potassium Dihydrogen Phosphate 6.8 g + Water 900 mL + Triethylamine 10 mL + Phosphoric Acid (→pH 3.5) + Water (→ 1 L).
* (W) and (D) show joint type: (W): Waters type; (D): DuPont type)
39
Cru
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stProduct Name Wako Cat. No. Chemical Structural Formula
Sennoside A Standard, 98.0+% (HPLC) 194-16241
C42H38O20 = 862.74CAS No. 81-27-6
Crude Drug Pkg. Size
・Sennae Folium・Rhei Rhizoma
10 mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
TLC HPLC
Sample: 1 mg/mL (Tetrahydrofuran 7 + Water 3 (volume ratio))Sample spotted: 20 μLThin-layer plate: Silicagel 70 FM Plate-Wako, 20 x 5 cm [Wako Cat. No.194-08394]Developing solvent: Ethyl Acetate 40 + 1-Propanol 40 + Water 30
+ Acetic Acid 1 (volume ratio)Distance spot travels:abt. 15 cmDetection method: UV 365 nm
Sample: 0.1 mg/mL (Eluent)Injection: 10 μLColumn: Wakosil-II 5C18 HG, 4.6 mmφ × 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 50℃Eluent: [Acetic Acid・Sodium Acetate buffer (pH 5.0) 1) 17 + Acetonitrile 8 (volume ratio)] 1 L
+ Tetra-n-heptylammonium Bromide 2.45 gFlow rate: 0.7 mL/minDetector: UV 340 nm
1) [Sodium Acetate Trihydrate 13.6 g + Water ( → 100 mL)] + [Acetic Acid 6 g + Water (→100 mL)](→pH 5.0) →Take the 100 mL + Water (→1 L).
Product Name Wako Cat. No. Chemical Structural Formula
Sennoside B Standard, 98.0+% (HPLC) 197-16091
C42H38O20 = 862.74CAS No. 128-57-4
Crude Drug Pkg. Size
・Sennae Folium・Rhei Rhizoma
10 mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
TLC HPLC
Sample: 1 mg/mL (Tetrahydrofuran7 + Water 3 (volume ratio))Sample spotted: 20 μLThin-layer plate: Silicagel 70 FM Plate-Wako, 20 x 5 cm [Wako Cat. No.194-08394]Developing solvent: Ethyl Acetate 7 + 1-Propanol 7 + Water 4
+ Formic Acid 2 (volume ratio)Distance spot travels: abt. 15 cmDetection method: UV 365 nm
Sample: 0.1 mg/mL (Eluent)Injection: 10 μLColumn: Wakosil-II 5C18 HG, 4.6 mmφ × 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 50℃Eluent: [Acetic Acid・Sodium Acetate buffer (pH 5.0) 1) 17 + Acetonitrile 8 (volume ratio)] 1 L
+ Tetra-n-heptylammonium Bromide 2.45 gFlow rate: 0.7 mL/minDetector: UV 340 nm
1) [Sodium Acetate Trihydrate 13.6 g + Water ( → 100 mL)] + [Acetic Acid 6 g + Water ( → 100 mL)](→ pH 5.0) →Take the 100 mL + Water (→ 1 L).
Product Name Wako Cat. No. Chemical Structural Formula
Shikonin (mixture of optical isomers, abt. 6 : 1), 98.0+% (HPLC)
198-13581
C16H16O5 = 288.30CAS No. 517-89-5
Crude Drug Pkg. Size
Lithospermum erythrorhizon 10 mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
TLC HPLC
Sample: 5 mg/mL (Acetone) Sample spotted: 5 μLThin-layer plate: Silicagel 70 F254 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08404]Developing solvent: Ethyl Acetate 3 + Ethanol (95) 1 (volume ratio)Distance spot travels: abt. 10 cmDetection method: Visual judgment and UV exposure
Sample: 1 mg/mL (Methanol) Injection: 5 μLColumn: CHIRALCEL OJ-H, 4.6 mmφ × 25 cmColumn Temperature: 40℃Eluent: Hexane 95 + 2-Propanol 5 + Acetic Acid 0.3 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 273 nm
for other products, please visit the Wako Online Catalog www.e-reagent.com
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TestProduct Name Wako Cat. No. Chemical Structural Formula
Shikonin (mixture of optical isomers, abt. 1 : 1), 98.0+% (HPLC)
198-13601
C16H16O5 = 288.30CAS No. 54952-43-1
Crude Drug Pkg. Size
Lithospermum erythrorhizon 10 mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
TLC HPLC (1)
Sample: 5 mg/mL (Acetone)Sample spotted: 5 μLThin-layer plate: Silicagel 70 F254 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08404]Developing solvent: Ethyl Acetate 3 + Ethanol (95) 1 (volume ratio)Distance spot travels: abt. 10 cmDetection method: ①Visual judgment ②UV exposure
Sample: 1 mg/mL (Methanol) Injection: 5 μLColumn: CHIRALCEL OJ-H, 4.6 mmφ × 25 cmColumn Temperature: 40℃Eluent: Hexane 95 + 2-Propanol 5 + Acetic Acid 0.3 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 273 nm
HPLC (2)
Sample: 1mg/mL (Methanol)Injection: 5 μLColumn: Wakosil-II 5C18 HG,4.6 mmφ × 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 40℃Eluent: 2 vol% Phosphoric Acid 25 + Methanol 75 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 273 nmQuantitation: Percentage by area
Product Name Wako Cat. No. Chemical Structural Formula
Shikonin Standard, 99.0+% (HPLC) 191-13331
C16H16O5 = 288.30CAS No. 517-89-5
Crude Drug Pkg. Size
Lithospermum erythrorhizon 10 mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
TLC HPLC
Sample: 5 mg/mL (Acetone)Sample spotted: 5 μLThin-layer plate: Silicagel 70 F254 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08404]Developing solvent: Ethyl Acetate 3 + Ethanol (95) 1 (volume ratio)Distance spot travels: abt. 10 cmDetection method: UV exposure and visual judgment
Sample: 1 mg/mL (Methanol)Injection: 5 μLColumn: Wakosil-II 5C18 HG,4.6 mmφ × 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 40℃Eluent: 2 vol% Phosphoric Acid solution 25 +Methanol 75 (volume ratio)Flow rate: 1.0 mL/min.Detector: UV 273nm
Product Name Wako Cat. No. Chemical Structural Formula
Sinomenine Standard, 98.0+% (HPLC) 193-09361
C19H23NO4 = 329.39CAS No. 115-53-7
Crude Drug Pkg. Size
Sinomeni Caulis et Rhizoma 20 mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
TLC HPLC
Sample: 10 mg/mL (Chloroform)Sample spotted: 2 μLThin-layer plate: Silicagel 70 F254 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08404]Developing solvent: Chloroform 20 + Methanol 20
+ Ammonia Solution 1 (volume ratio)Distance spot travels: abt. 10 cmDetection method: UV exposure
Sample: 0.2 mg/mL (Eluent)Injection: 10 μLColumn: Wakosil 5C18,4.6 mmφ × 15 cm
[Wako Cat. No.: 239-53983 (W), 233-53981 (D)] *Column Temperature: Room temperatureEluent: Sodium Dodecyl Sulfate Solution (5 g/L) 11 + Acetonitrile 9 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 260 nm
* (W) and (D) show joint type: (W): Waters type; (D): DuPont type)
41
Cru
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stProduct Name Wako Cat. No. Chemical Structural Formula
Swertiamarin Standard, 98.0+% (HPLC) 198-16901
C16H22O10 = 374.34CAS No. 17388-39-5
Crude Drug Pkg. Size
Swertia Herb 20mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
HPLC
Sample: 2 mg/ 10 mL (Eluent)Injection: 10μLColumn: Wakosil-II 5C18HG, 4.6 mmφ×15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 40℃Eluent: Acetonitrile 10 + Water 90 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 238nm
Product Name Wako Cat. No. Chemical Structural Formula
Tauroursodeoxycholic Acid Standard, 98.0+% (HPLC)
203-17061
C26H45NO6S = 499.70CAS No. 14605-22-2
Crude Drug Pkg. Size
Fel Ursi 20mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
TLC HPLC
Sample: 10 mg/mL (Methanol)Sample spotted: 5 μLThin-layer plate: Silicagel 70 Plate-Wako, 20 × 5 cm [Wako Cat. No.197-08384]Developing solvent: Toluene 10 + Acetic Acid 10 + Water 1 (volume ratio)Distance spot travels: abt. 10 cmDetection method: Dry at RT.→Spray Sulfuric Acid (1+18) →Heat at 105℃ for 10 min.
→ Visual judgment.
Sample: 0.5 mg/mL (Methanol)Injection: 10 μL Column: Wakosil-II 5C18 HG, 4.6 mmφ × 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 40℃Eluent: 20 mmol/L Phosphate buffer (pH 3.0) 1) 70 + Acetonitrile 30 (volume ratio)Flow rate: 1.0 mL/minDetector: UV 200 nm
1) Potassium Dihydrogen Phosphate1.36 g + Water 450 mL + Phosphoric Acid (→pH 3.0) + Water (→500 mL).
Product Name Wako Cat. No. Chemical Structural Formula
Timosaponin A-III Standard, 98.0+% (HPLC)
207-16861
C39H64O13 = 740.92CAS No. 41059-79-4
Crude Drug Pkg. Size
Anemarrhenae Rhizoma 20 mg
Grade Storage
for Crude Drugs Determination Keep at 2-10℃.
Example analysis
TLC HPLC
Sample: 1 mg/mL (Methanol)Sample spotted: 5 μLThin-layer plate: Silicagel 70 Plate-Wako, 20 x 5 cm [Wako Cat. No.197-08384]Developing solvent: 1-Propanol 7 + Ammonia Solution 3 (volume ratio)Distance spot travels: abt. 10 cmDetection method : Dry at RT.→ Spray [Sulfuric Acid 1 + Ethanol (99.5) 20 (volume
ratio)]→ Dry at RT. → Heat at 105℃ for 5 min.→Visual judgment.
Sample: 10 mg/mL (DMSO 3 + Methanol 7)Sample spotted: 20 μLColumn: Wakosil-II 5C18 HG, 4.6 mmφ × 15 cm
[Wako Cat. No.: 234-51113 (W), 238-51111 (D)] *Column Temperature: 40℃Eluent: Water 50 + Acetonitrile 50 (volume ratio)Flow rate: 1.0 mL/minDetector: Differential refractometer detector
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42
Product Name PAGE
Index
AAchyranthes Root ···························· 1, 20Aconitine Standard ······························ 20Aconitum Diester Alkaloids Standard ······· 1Aconitum Monoester Alkaloids Standard ····· 1Acteoside Standard ····························· 20Albiflorin ················································· 1Alisol A ··················································· 1Alisol B Acetate ··································· 21Alisol B Standard ································· 21Alkannin Standard ······························· 21Amygdalin ·············································· 2Arbutin ··················································· 3Arctiin Standard ··································· 22Artemisia Argyi ································ 3, 22Asarinin ·················································· 2(E )-Asarone ··········································· 2Astragaloside IV ···································· 2Atractylenolide III ··································· 2Atractylodin Standard ·························· 22Atractylodin TS ······································ 3Atropine Sulfate Hydrate ······················· 3Aucubin Standard ································ 23
BBaicalein Standard ······························ 23Baicalin ·················································· 3Barbaloin ··············································· 4Benz [a] anthracene ······························· 4Benzo [a] pyrene ···································· 4Benzoylmesaconine Hydrochloride ······· 4Berberine Chloride Hydrate ··················· 4Bergenin ················································ 5Bisdemethoxycurcumin ························· 5Bufalin ··················································· 5Bufotalin Standard ······························· 23
CCalycosin 7-O-Glucoside Standard ······· 24(E )-Capsaicin ········································· 5Carbazochrome Sodium Sulfonate Trihydrate ············································· 24Catalpol Standard ······························· 24Chikusetsusaponin IV ···························· 5(E )-Chlorogenic Acid ····························· 6(E )-Cinnamaldehyde ······························ 6(E )-Cinnamic Acid ································· 6Cinobufagin ··········································· 6Cinobufotalin Standard ······················· 25Coptisine Chloride ································· 6Corosolic Acid Standard ····················· 25Corydaline Standard ···························· 25Curcumin ··············································· 7Cycloartenyl Ferulate ····························· 7
DDehydrocorydaline Nitrate ····················· 7Dehydrocostus Lactone Standard ······· 26Demethoxycurcumin ····························· 7Dihydrocapsaicin Standard ················· 265,7-Dimethoxyflavone Standard ·········· 262,6-Dimethoxyphenol Standard ·········· 27Dimethylesculetin Standard ················ 27
EEchinacoside Standard ······················· 27
Eleutheroside B ····································· 7Emetine Dihydrochloride Standard ····· 28Epihesperidin Standard ······················· 28Ergosterol Standard ····························· 28β-Eudesmol Standard ························· 29Evodiamine Standard ·························· 29
F(E )-Ferulic Acid ······································ 8Fructose ················································ 8
GGeniposide ············································ 8Geniposidic Acid Standard ·················· 29Gentiopicroside ····································· 8[6]-Gingerol ············································ 8[6]-Gingerol Standard ·························· 30[8]-Gingerol Standard ·························· 30[10]-Gingerol Standard ························ 30Ginsenoside Re ····································· 9Ginsenoside-Rb1 Standard ················· 31Ginsenoside Rc ····································· 9Ginsenoside-Rd Standard ··················· 31Ginsenoside-Rg1 ··································· 9Glabridin ·············································· 314'-O-Glucosyl-5-O-methylvisamminol ····· 9Glycocholic Acid Standard ·················· 32Glycohyodeoxycholic Acid Standard ····· 32Glycyrrhizic Acid ···································· 9Glycyrrhizic Acid Standard ·················· 32Gomisin A Standard ···························· 33Gomisin N Standard ···························· 33Guaiacol ·············································· 10
HHesperidin ··········································· 10Hirsutine ·············································· 10Honokiol ·············································· 1010-Hydroxy-2-(E )-decenoic Acid ········ 103-(3-Hydroxy-4-methoxyphenyl)-2-(E )-propenoic Acid ···································· 11Hyodeoxycholic Acid ··························· 11Hypaconitine ······································· 33Hyperoside ·········································· 11
IIcariin ··················································· 11Isoacteoside Standard ························ 34Isofraxidine Standard ·························· 34
L(Z)-Ligustilide Standard Solution ········· 34Limonin ················································ 11Liquiritin ··············································· 12Loganin ················································ 12Luteolin ················································ 12
MMagnoflorine Iodide Standard ············· 35Magnoflorine Standard ························ 35Magnolol ·············································· 12Manninotriose ······································ 12l-Menthol ············································· 13Mesaconitine ······································· 35(E )-2-Methoxycinnamaldehyde ··········· 13Myristicin ············································· 13
NNaringin ··············································· 13Neohesperidin Standard ····················· 36Nodakenin ··········································· 13
Oγ-Oryzanol Standard ··························· 36Osthole ················································ 14Oxymatrine Standard ·························· 36
PPaeoniflorin ·········································· 14Paeonol ··············································· 14Palmatine Chloride ······························ 14Perillaldehyde ······································ 14Plantago Seed ······························· 15, 37Powdered Swine Bile ·························· 15(±)-Praeruptorin A ······························· 15Praeruptorin A Standard ······················ 37Puerarin ··············································· 15
RResibufogenin ····································· 15Rhaponticin Standard ·························· 37Rhein ··················································· 16Rhynchophylline ·································· 16Rosmarinic Acid ·································· 16Rutin Standard ···································· 38
SSaikosaponin a ···································· 16Saikosaponin b2 ··································· 16Saikosaponin c Standard ···················· 38Saikosaponin d ···································· 17Schizandrin ·········································· 17Scopolamine Hydrobromide n-Hydrate Standard ·············································· 38Scopoletin ··········································· 17Sennoside A Standard ························ 39Sennoside B Standard ························ 39Sesamin ··············································· 17Shikonin ········································· 39, 40Shikonin Standard ······························· 40[6]-Shogaol ·········································· 17Sinomenine Standard ·························· 40Sodium Glycocholate ·························· 18Sodium Tauroursodeoxycholate ·········· 18Stachyose ············································ 18Swertiamarin ······································· 18Swertiamarin Standard ························ 41
TTauroursodeoxycholic Acid Standard ····· 41Theophylline ········································ 18Timosaponin A-III Standard ················· 41
UUmbelliferone ······································ 19
VVerbascoside ······································· 20
WWogonin ·············································· 19
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