Download - Genome Annotation with Companion (Part 2)...Genome Annotation with Companion (Part 2) You should have gotten an email indicating the status of your annotation (ie. job started and

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Page 1: Genome Annotation with Companion (Part 2)...Genome Annotation with Companion (Part 2) You should have gotten an email indicating the status of your annotation (ie. job started and

GenomeAnnotationwithCompanion(Part2)Youshouldhavegottenanemailindicatingthestatusofyourannotation(ie.jobstartedandjobcomplete).Theemailcontainsalinktotheannotationoutput.

• Exploreyourresultswithyourgroupanddiscusstheannotationfindings:

o Whatdoesthegenomestaticstabtellyouaboutyourannotation?Aretheresultssurprising?YoucanexplorethereferencegenomeyouusedinEuPathDBtohelpyouassesstheresults.(Forexample,areyougettingareasonablenumberofgenes?WhatabouttheGCcontent?Numberofnon-codinggenes?)

Page 2: Genome Annotation with Companion (Part 2)...Genome Annotation with Companion (Part 2) You should have gotten an email indicating the status of your annotation (ie. job started and

o Whatdoesthe“Resultfiles”tabcontain?WhatisanAGPfile?WhatisaGFF3file?

o Whatdoesthe“orthology”tabdisplay?Howmanypredictedproteins

fromyournewgenomeareincommonwithonesfromthereferencegenome?Howmanyareuniquetoyours?Whatdosingletonsrepresent(clickonthesingletonnumbertoseewhatthesegenesare?

Page 3: Genome Annotation with Companion (Part 2)...Genome Annotation with Companion (Part 2) You should have gotten an email indicating the status of your annotation (ie. job started and

o Whatdoesthephylogenytabrepresent?Doesitmakesense?

o ExaminetheSyntenytab–arethesegenomessyntenic?