EXS Experientia Supplementum Vol. 52
Springer Basel AG
Metallothionein II Proceedings of the «Second International Meeting on Metallothionein and Other Low Molecular Weight Metal-binding Proteins», Ziirich, August 21-24, 1985
1987
Edited by
Jeremias H.R. Kagi Yutaka Kojima
Springer Basel AG
IUB Symposium No. 148
CIP-Kurztitelaufnahme der Deutschen Bibliothek
Metallothionein : proceedings of the ... Internat. Meeting on Metallothionein and Other Low Molecular Weight Metal-binding Proteins.
1 mit d. Erscheinungsorten Basel, Boston, Stuttgart NE: International Meeting on Metallothionein and Other Low Molecular Weight Metal Binding Proteins 2. Ziirich, August 21-24, 1985.- 1987.- ca. 702 S. (Experientia : Supplementum ; Voi. 52) (IUB symposium ; No. 148) ISBN 978-3-0348-6786-3
NE: Experientia 1 Supplementum; International Union of Biochemistry: IUB symposium
Ali rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical, photocopying, recording or otherwise, without the prior permission of the copyright owner.
© 1987 Springer Basel AG Originally published by Birkhiiuser Verlag Basel in 1987 Softcover reprint of the hardcover lst edition 1987
ISBN 978-3-0348-6786-3 ISBN 978-3-0348-6784-9 (eBook)
DOI 10.1007/978-3-0348-6784-9
v
PREFACE
Metallothioneins, ubiquitous low molecular weight proteins of extremely high metal and sulfur content, are of great interest to various branches of the life sciences ranging from bioinorganic chemistry to biochemistry, molecular biology, physiology, toxicology environmental science and medicine. They are believed to play a pivotal role in reg- ' ulating the flow of the essential trace elements zinc and copper through the cell and in modulating the harmful environmental influences of toxic metals and of various stress conditions. Since the first international meeting held on this subject in 1978 the knowledge of the structure and the function of these proteins has been deepened and extended to molecular dimensions. Thus, the spatial structure of the protein and the mode of metal binding have now been established by x-ray diffraction and advanced NMR and optical techniques. The discovery and identification of unique genetic switching mechanisms opened the way for an understanding of the characteristic inducibility of this protein by metals and hormones and led to the utilization of the metallothionein genes as regulatory devices in genetic engineering and in transgenic experiments.
The Second International Meeting on Metallothionein and Other Low Molecular Weight Metal-binding Proteins held in Zurich, Switzerland, from August 21 - 24, 1985, was arranged as an official Satellite Meeting - IUB Symposium If 148 - of the 13th International Congress of Biochemistry, Amsterdam, The Netherlands, and hosted by the Institute of Biochemistry of the University of Zurich. It was sponsored jointly by the International Union of Biochemistry (IUB), the International Union for Pure and Applied Biophysics (IUPAB), the World Health Organization (WHO), the Scientific C.ommittee on the Toxicology of Metals under the Permanent Commission and International Association of Occupational Health, the Schweizerischer Nationalfonds, the Schweizerische Naturforschende Gesellschaft, the National Academy of Sciences (USA), the Biological Division of the American Chemical Society, the Society of Toxicology (USA), and the biochemical societies of various countries (American Society of Biological Chemists, Australian Biochemical Society, Gesellschaft fUr Biologische Chemie (Germany), the Japanese Biochemical Society, and the Schweizerische Gesellschaft fUr Biochemie.
The meeting was attended by about 200 scientists from 21 countries including representatives from the International Programme on Chemical Safety of WHO, the Metals Specialty Section of the Society of Toxicology (USA), and the Scientific Committee on the Toxicology of Metals under the Permanent Commission and International Association of Occupational Health. The Introductory Lecture was given by the Honorary President, Professor Bert L. Vallee, on the topic "Implications of Metallothionein Chemistry". The scientific contributions were organized in six symposia, six colloquia and poster presentations and discussions.
This book comprises the proceedings of the meeting. Part I contains the introductory lecture, recommendations on the nomenclature on metallothionein and solicited review articles on the biochemical, molecular -biological, physiological, and toxicological aspects of metallothionein intended to facilitate access to this interdisciplinary field. Part II consists of contributions in symposia, colloquia and poster discussions. Part III contains the program of the meeting and the poster abstracts.
On behalf of the organizer and the participants, we acknowledge the generous financial support received from the Schweizerischer Nationalfonds, the Schweizerische Naturforschende Gesellschaft, the Union Schweizerischer Gesellschaften fUr Experimentelle Biologie, the Erziehungsdirektion des Kantons Zurich, the Krebsliga des Kantons
VI
Zurich, the National Institute of Environmental Health Sciences (USA), the United States Department of Energy, the International Union of Biochemistry (IUS), the International Union for Pure and Applied Biophysics (IUPAB), and the Society of Toxicology Metals Specialty Section (USA). Our sincere thankfulness also goes to the University of Zurich for providing the facilities for the meeting.
We also acknowledge the assistance of the editorial staff of Birkhauser Verlag, Basel/ Boston/Stuttgart. Special thanks also to Ms. Genia de Vallier for her devoted effort in the preparation and organization of the meeting and in the production of this volume.
April, 1987
Jeremias H.R. Kagi
Yutaka Kojima
TABLE OF CONTENTS
Preface
Part I
Lecture by the Honorary President
Implications and Inferences of Metallothionein Structure B.L. Vallee
Recommendations on Nomenclature of Metallothionein
Nomenclature of Metallothionein B.A. Fowler, C.E. Hildebrand, Y. Kojima, and M. Webb
Reviews on Metallothionein
Chemistry and Biochemistry of Metallothionein J.H.R. Kagi and Y. Kojima
Molecular Biology of Metallothionein Gene Expression R.D. Palmiter
Nutritional and Physiological Significance of Metallothionein I. Bremner
Toxicological Significance of Metallothionein M. Webb
Part II
3
5
17
19
23
25
63
81
109
Chemical Structure of Metallothionein 137
Crystal Structure of Cd,Zn Metallothionein 139 W.F. Furey, A.H. Robbins, L.L. Clancy, D.R. Winge, B.C. Wang, and C.D. Stout
Spatial Structure of Rabbit Liver Metallothionein-2 149 in Solution by NMR
G. Wagner, M.H. Frey, D. Neuhaus, E. Wi:irgi:itter, w. Braun, M. vasak, J.H.R. Kagi, and K. Wuthrich
NMR Analysis of the Structure and Metal Sequestering 159 Properties of Metallothioneins
I.M. Armitage, D.C. Dalgarno, and B.A. Johnson
Interprotein Metal Exchange Reactions of Metallothionein 171 J.D. Otvos, H.R. Engeseth, D.G. Nettesheim, and C.R. Hilt
Spectroscopic and Chemical Approaches to the Study of 179 Metal-Thiolate Clusters in Metallothionein (MT)
M. Vasak, J. Overnell and M. Good
VII
VIII
Probing the Reactivity of the Zinc and Cadmium Ions 191 Bound to Rabbit Liver Metallothloneins with EDT A
J.K. Nicholson, P.J. Sadler and M. Vasak
Information on Metal Binding Properties of Metallothioneins 203 from Optical Spectroscopy
M.J. Stillman, A.Y.C. Law, W. Cai, and A.J. Zelazowski
Copper Coordination in Metallothionein 213 D.R. Winge
Structural and Functional Aspects of Metal-Thiolate 219 Centres in Metallothionein
U. Weser
EXAFS Studies of Metallothionein 227 S.S. Hasnain, G.P. Diakun, I. Abrahams, I. Ross, C.D. Garner, I. Bremner, and M. Vasak
Luminescence Emission from the Cu(I)-Thiolate Complex 237 in Metallothioneins
M. Beltramini, K. Munger, U.A. Germann, and K. Lerch
Cadmium Binding and Metal Cluster Formation in 243 Metallothionein: A Differential Modification Study
w .R. Bernhard, M. Va~ak and J.H.R. Kagi
Metallothionein: A Bifunctional Chelator for the 247 Radiolabeling of Biologically Active Molecules
M.M. Morelock and G.L. Tolman
Biochemistry of Metallothionein 255
Human Hepatic Metallothioneins: Resolution of Six Isoforms 257 P.E. Hunziker and J.H.R. Kagi
Quantification and Characterization of Metallothioneins 265 in Tissues of Lower Vertebrates and Invertebrates
K.T. Suzuki
Cadmium-Binding Proteins in Rat Testes: Characterization 273 and Apparent Source of the Low-Molecular-Weight Protein
C.D. Klaassen and M.P. Waalkes
Low Molecular Weight Cadmium and Selenium Containing 281 Proteins Unlike Metallothionein in Animals
P.O. Whanger, J.T. Deagen and M.A. Beilstein
Characterization of Metallothionein-Like Protein in Rat Brain 289 M. Ebadi and S. Swanson
Cadmium Resistance in Drosophila: A Small Cadmium Binding 293 Substance
K.B. Jacobson, M.W. Williams, L.J. Richter, S.E. Holt, G.J. Hook, S.M. Knoop, F.V. Sloop, and J.B. Faust
The Cd-Binding Protein from Tomato Compared to those 301 of Other Vascular Plants
W.E. Rauser
Purification and Characterization of Atypical 309 Cadmium-Binding Polypeptides from Zea Mays
W .R. Bernhard and J.H.R. Kagi
Phytochelatins, the Heavy Metal Binding Peptides of Plants: 317 Characterization and Sequence Determination
E. Grill
Characterization of Metal Binding Peptides from 323 Cadmium Resistant Plant Cells
N.J. Robinson, K. Barton, C.M. Naranjo, L.O. Sillerud, J. Trewhella, K. Watt, and P.J. Jackson
Electrochemical Quantification and Characterization 329 of Metallothioneins
R.W. Olafson
Enzyme-Linked Immunosorbent Assay (ELISA) for 335 Metallothionein
J.S. Garvey, D.G. Thomas and H.J. Linton
Comparison of Cadmium-Saturation Assay and 343 Radioimmunoassay for the Determination of Metallothionein Concentration in Tissues
Z.A. Shaikh and C.V. Nolan
Metallothionein-Determination in Biological Materials: 351 Interlaboratory Comparison of 5 Current Methods
H.H. Dieter, L. MUller, J. Abel, and K.H. Summer
Biochemical Genetics of Metallothionein 359
Structure and Expression of the Human Metallothionein Genes 361 L. Gedamu, U. Varshney, N. Jahroudi, R. Foster, and N. W. Shworak
Rat Metallothionein Multigene Family 373 R.D. Andersen, S.J. Taplitz, B.W. Birren, G. Bristol, and H.R. Herschman
The Metallothionein Gene of Drosophila 385 G. Maroni, E. Otto, D. Lastowski-Perry, and D.H. Price
Isolation and Regulation of Expression of the Neurospora 393 crassa Copper Metallothionein Gene
K. MUnger, U.A. Germann and K. Lerch
Transcriptional Control Mechanisms which Regulate the 401 Expression of Human Metallothionein Genes
M. Karin, A. Haslinger, A. Heguy, T. Dietlin, and R. Imbra
Metal Regulatory Elements of the Mouse Metallothionein-I 407 Gene
P.F. Searle, G.W. Stuart and R.D. Palmiter
Inducible and Constitutive Sequence Elements in the 415 Enhancer of the Mouse Metallothionein-I Gene
E. Serfling and W. Schaffner
IX
X
Function and Regulation of Yeast Copperthionein 423 D.J. Thiele, C.F. Wright, M.J. Walling, and D.H. Hamer
Synaptic Relations in Meiotic Gene Conversion at the 431 Iterated CUP1r Locus of S. cerevisiae
J.W. Welch, D.H. Maloney and S. Fogel
Role of Metallothionein in Detoxification and Tolerance 439 to Transition Metals
P.C. Huang, S. Morris, J. Dinman, R. Pine, and B. Smith
Molecular and Cellular Mechanisms of Cadmium 447 Resistance in Cultured Cells
D.L. Grady, R.K. Moyzis and C.E. Hildebrand
Analysis of the Detoxification of Heavy Metal Ions by 457 Mouse Metallothionein
D.M. Durnam and R.D. Palmiter
Heavy Metal Induced Protein Synthesis in Fish Cell Lines 465 J. Price-Haughey and L. Gedamu
Injection of the Mouse MT -1 Gene into Rainbow Trout Eggs 471 and Assay of Trout Fry for Resistance to Cadmium and Zinc Toxicity
N. Maclean, C. Woodall and F. Crossley
Abnormal Copper Metabolism and Regulation of 477 Metallothionein Gene Expression in Menkes' Disease
A. Leone and D.H. Hamer
Role of Metallothionein in Metal Pathways and Cell Metabolism 481
Metallothionein in Regeneration, Reproduction and 483 Development
M. Webb
Biosynthesis and Metabolism of Metallothionein in 499 Rat during Perinatal Development
M.G. Cherian, D.M. Templeton, K.R. Gallant, and D. Banerjee
Metallothionein in Blood, Bile and Urine 507 I. Bremner, R.K. Mehra and M. Sato
Metallothioneins of Monocytes and Lymphocytes 519 S. Koizumi, T. Sane, M. Kimura, F. Otsuka, and M. Ohsawa
Synthesis and Degradation of Erythrocyte Metallothionein 525 in Cadmium-Administered Mice
K. Tanaka, K. Min, S. Onosaka, ar1d C. Fukuhara
Histological Demonstration of Immunoreactive Metallothionein 533 in Rat and Human Tissues
M.E. Elmes, J.P. Clarkson and B. Jasani
The Function of Metallothionein in Cell Metabolism 539 D.G. Thomas, A.D. Dingman and J.S. Garvey
Regulation of Liver Zinc Metabolism and Metallothionein 545 by cAMP, Glucagon and Glucocorticoids and Suppression of Free Radicals by Zinc
R.J. Cousins and D.E. Coppen
The Involvement of Catecholamines and Polypeptide 555 Hormones in the Multihormonal Modulation of Rat Hepatic Zinc Thionein Levels
F.D. Brady, B.S. Helvig, A.E. Funk, and S.H. Garrett
Induction of Synthesis and Degradation of Metallothionein-I 565 in the Tissues of Rats Injected with Zinc
R.K. Mehra and I. Bremner
Cadmium-Zinc Interactions in the Ehrlich Cell: 573 Metallothionein and other Sites
D.H. Petering, S. Krezoski, J. Villalobos, C.F. Shaw III, and J.D. Otvos
Effects of Type I Interferon-Inducing Agents on Hepatic 581 Metallothionein
J.U. Bell, M.J.P. Lawman, J.M. Lopez, L.E. DesJardin, and L.A.D. Applewhite
Metallothionein and Zinc Metabolism in Endotoxin Shock Rats 587 S. Abe, M. Matsumi, M. Tsukioki, S. Mizukawa, T. Takahashi, Y. Iijima, Y. Itano, and F. Kosaka
Protective Function of Metallothionein against Certain 595 Anticancer Agents
L. Endresen and H.E. Rugstad
Alteration of Radiosensitivity in Metallothionein Induced Mice 603 and a Possible Role of Zn-Cu-Thionein in GSH-Peroxidase System
J. Matsubara
Activation of o-Aminolevulinic Acid Dehydratase Following 613 Donation of Zinc from Kidney Metallothionein
P.L. Goering and B.A. Fowler
Role of Metallothionein in Metal Detoxification, Metal Tolerance 617 and Metal Toxicity
Role of Metallothionein in Induced Resistance to Cadmium 619 Toxicity in Isolated Rat Hepatocytes
J.M. Frazier and W .S. Din
Metallothionein Gene Expression and Cadmium Toxicity in 627 Freshwater Fish
J. Kay, M.W. Brown, A. Cryer, J.F. del.G.Solbe, D. Shurben, J.S. Garvey, and D.G. Thomas
Role of Metallothionein in Metal Detoxification and Metal 631 Tolerance in Protein Calorie Malnutrition and Calcium Deficient Monkeys (Macaca mulatta)
R. Nath, V.K. Paliwal, R. Prasad, and R. Kambadur
XI
XII
Intestinal Absorption of Cadmium and Inorganic Mercury in 639 the Rat: No Major Involvement of Metallothionein
J.K. Piotrowski, J.A. Szymar'iska, B.W. Kaszper, M. Skrzypir'iska-Gawrysiak, and S. Brzeznicki
Hepatic Lysosomal Copper-Thionein 647 I. Sternlieb
Induction of Renal Metallothionein Allows Increasing Dose of 655 an Extensively Used Antitumor Drug, cis-Diamminedichloro-platinum - ·
N. Imura, A. Naganuma, M. Satoh, and Y. Koyama
Mechanism of Cadmium-Metallothionein-Induced Nephro- 661 toxicity: Relationship to Altered Renal Calcium Metabolism
B.A. Fowler, P.L. Goering and K.S. Squibb
On the Role of Metallothionein in Cadmium Induced Renal 669 Toxicity
M. Nordberg and G.F. Nordberg
Cadmium, Zinc and Copper in Rabbit Kidney Metallothionein - 677 Relation to Kidney Toxicity
Part III
C.G. Elinder, M. Nordberg, B. Palm, L. Bjorck, and L. Jonsson
Program of the Second International Meeting on Metallothionein and Other Low Molecular Weight Metal-binding Proteins
Poster Abstracts
List of Participants
Index
Author Index
Subject Index
683
695
731
743
747
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