Effects of UV-B on the growth of Vigna radiata ( 綠豆 ), Vigna
angularis ( 紅豆 ) and Glycine max ( 大豆 )
ST. PAUL’S CO-EDUCATIONAL COLLEGECHUI HO YIN PAUL
CHAN YUN YIN CYRUSLEE WAI LAM BRYAN
Hey! Dear, I have a fabulous yield this year!!!
Dad! We may get a poor yield next
ten years!!!
WHY!?!?!
光 禿禿?
Observations and facts
Ozone concentration decreases
– Higher amount of ultraviolet-B (UVB) radiation reaching the earth
– cause harmful effects to the biosphere
Literature ReviewThe effect of UVB on plants
Negative impacts of UVB on Glycine max:– Decrease the areas and the number of leaves
– Decrease height of the stems
– Decrease the photosynthetic rates and the evaporation rate
– Delay the growth process
Problem??
Will the yield of the crops be reduced???
Objectives
1. To find out the effect of UVB radiation on 3 types of plants (Vigna radiata 綠豆 , Vigna angularis 紅豆 and Glycine max 大豆 )
2. To find out which of these three plants has stronger resistance to the damaging effect of UVB radiation
Hypothesis
Under extra UVB:
The growth processes would be delayed
Fewer leaves would be developed
Shorter stems would be developed
Set-up 1BVigna angularis
Set-up 1CGlycine max
Set-up 1AVigna radiata
Set-up 2BVigna angularis
Set-up 2CGlycine max
Set-up 2AVigna radiata
Methodology
Set-up Type of seed Volume ofwater added
daily (cm³)
Number of seeds
Set-up 1 (control set-
up)
1 A Vigna radiata
綠豆84 40
1 B Vigna angularis
紅豆84 40
1 C Glycine max
大豆84 40
Set-up 2 2 A Vigna radiata
綠豆84 40
2 B Vigna angularis
紅豆84 40
2 C Glycine max
大豆84 40
Variable TableControlled variables Independent variables Dependent variables
Number of seeds used
Number of cotton layers used
Volume of water poured everyday
Types of plants
Illumination treatment
Number of leaves
Length of stems
Colour of leaves
Illumination treatment
Setup 1
7:00 18:00Sunlight
Setup 2
7:00 18:0013:00 16:00Sunlight
Sunlight + UV (300nm-400nm)
Sunlight
Result
Setup 1B Setup 1C Setup 1A
Setup 2B Setup 2C Setup 2A
The average number of leaves of Vigna radiata 綠豆with or without extra UVB
0
0.2
0.4
0.6
0.8
1
1.2
1.4
1.6
1.8
0 5 10 15 20 25
Day
Ave
rage
num
ber o
f lea
ves
withoutextra UVB
with extraUVB
The growth of Vigna radiata 綠豆with or without extraUVB
0
5
10
15
20
25
0 5 10 15 20 25 Day
The
leng
th o
f Vig
na r
adia
ta (
cm)
withoutextra UVB
with extraUVB
The average number of leaves of Vigna angularis 紅豆with and without extra UVB
0
0.2
0.4
0.6
0.8
1
1.2
1.4
1.6
0 5 10 15 20 25 Day
Ave
rage
num
ber
of le
aves
withoutextraUVB
with extraUVB
The growth of Vigna angularis 紅豆 with or withoutextra UVB
0
5
10
15
20
25
30
35
0 5 10 15 20 25 Day
The
leng
th o
f Vig
na a
ngul
aris
(cm
)
withoutextra UVB
with extraUVB
The average number of leaves of Glycine max 大豆with or without extra UVB
0
0.2
0.4
0.6
0.8
1
1.2
1.4
0 5 10 15 20 25 Day
Ave
rage
num
ber o
f lea
ves without
extraUVB
withextraUVB
The growth of Glycine max 大豆 with or withoutextra UVB
0
5
10
15
20
25
30
0 5 10 15 20 25 Day
The
leng
th o
f Gly
cine
max
(cm
)
withoutextraUVB
withextraUVB
Colour of leaves
• Without extra UVB (Set-up 1):– green
• With extra UVB (Set-up 2):– green → yellow → brown →black (died)
CONCLUSION 1• Under extra UVB:
– Delayed germination
– Delayed foliation
– Fewer leaves developed
– Shorter stems developed
– Colour of the leaves changed from green to black
WHY??
Analysis
• Set-up 2– Green → Yellow → Brown →Black
• Maybe UVB blocks some nutrients from being absorbed by plants
– Slow down chlorophyll formation
– Cause the deficiency and poor growth
Further investigation• Measure the amounts of photodimer and photolyase
before and after UVB illumination
• Investigate the relationship between the UVB dosage and the amounts of photodimer formed
• Investigate the effect of UVB on the photosynthetic rate of plants
Which plant has the best resistance to
UVB???
The highest average number of leaves developed in theset-ups with and without extra UVB
0
0.2
0.4
0.6
0.8
1
1.2
1.4
1.6
1.8
Vigna radiata Vigna angularis Glycine max
Hig
hes
t av
erag
e n
um
ber
leav
es r
ecor
ded
without extraUVB
with extraUVB
10.7
0.6
Both Glycine max (大豆 ) & Vigna angularis(紅豆 ) had shown small
difference
The day of first foliation with and without extra UVB
0
2
4
6
8
10
12
Vigna radiata Vigna angularis Glycine max
Day
of
firs
t fo
liat
ion
without extra UVBwith extra UVB1
5
4
Vigna radiata綠豆 had the smallest difference
The day of germination with and without extra UVB
0
2
4
6
8
10
12
Vigna radiata Vigna angularis Glycine max
Day
of
germ
inat
ion
without extraUVBwith extra UVB1
53
Vigna radiata綠豆 had the smallest difference
The highest average height recorded in the set-ups with andwithout extra UVB
0
5
10
15
20
25
30
35
Vigna radiata Vignaangularis
Glycine max
Hig
hest
ave
rage
hei
ght r
ecor
ded
(cm
)
withoutextraUVBwith extraUVB
10.3
1311.6
Vigna radiata綠豆 had the smallest difference
The day at which the plants grown under extraUVB died
0
5
10
15
20
25
Vigna radiata Vigna angularis Glycine max
Day
that
the
seel
ding
die
d
with extra UVB
Vigna radiata ( 綠豆 ) in the set-up with UVB died latest among three set-ups.
CONCLUSION 2
• The growth of Vigna radiata(綠豆 )is least affected under extra UVB condition among the three plants
• Vigna radiata (綠豆 )has the best resistance to UVB among the three plants (Vigna radiata, Vigna angularis and Glycine max) studied
Further investigation• Investigate the effect of UVB dosage on the
growth of Vigna radiata (綠豆 )
• Investigate whether Vigna radiata (綠豆 )from different climatic regions show different resistance to UVB
In order to have a good harvest, is it more
beneficial for me to grow Vigna radiata?
I think…
THANK YOU!!!Please protect our
earth!!!!
REFERENCE1. 二氧化碳 , 臭氧 , 紫外輻射與農作物生產─氣象出版社
2. Elevated Ultraviolet (UV)-B Radiation and Agriculture. Springer Press.
3. Plants and UV-B─Responses to Environmental Change. Cambridge University Press.
4. Ozone crisis: the 15-year evolution of a sudden global emergency. John Wiley and Sons Press.
Websites5. http://earthobservatory.nasa.gov/Library/UVB/6.
http://www.bioon.com/biology/Class45/molecular/200406/53376.html
• 7. http://www.ciesin.org/docs/011-467/011-467.html8. http://www.mountcarmelhealth.com/healthinfo/Adult/skin/ultravio.shtml
9. http://uvb.nrel.colostate.edu/UVB/plant_impact/intro.html10. http://www.epa.gov/safewater/mdbp/pdf/alter/chapt_8.pdf11. http://www.people.com.cn/BIG5/huanbao/56/20010913/559617.html
12. http://www.npcc.gov.tw/nm-research/doctors/Concise_NM/%E7%AC%AC%E5%8D%81%E4%BA%8C%E7%AB%A0%20%20%20%E9%9B%BB%E9%9B%A2%E8%BC%BB%E5%B0%84%E7%9A%84%E7%94%9F%E7%89%A9%E5%AD%B8%E6%95%88%E6%87%89.htm
13. http://www.stut.edu.tw/epc/epc5/06(final).ppt
Comparison of the highest average number of leaves between the set-ups with (2A, 2B, 2C) and without UVB (1A, 1B, 1C)
Plant used Set-ups The highest average number of
leaves recorded
Difference between two
set-ups
Vigna radiata 1A (without UVB)
1.7 1
2A (with UVB) 0.7
Vigna angularis
1B (without UVB)
1.5 0.7
2B (with UVB) 0.8
Glycine max 1C (without UVB)
1.3 0.6
2C (with UVB) 0.7
Difference of the day of first foliation between the set-up with (2A, 2B, 2C) and without UVB (1A, 1B, 1C)
Plant used Set-ups Day of the first
foliation recorded
Difference between two set-ups
Vigna radiata - UVB 10 1+ UVB 11
Vigna angularis
- UVB 4 5
+ UVB 9
Glycine max - UVB 6 4
+ UVB 10
Difference of day of germination between the set-ups with (2A, 2B, 2C) and without UVB (1A, 1B, 1C)
Plant used Set-ups Day of germination
Difference between two
set-ups
Vigna radiata 1A (without UVB)
10 12A (with UVB) 11
Vigna angularis
1B (without UVB)
4 5
2B (with UVB) 9
Glycine max 1C (without UVB)
6 3
2C (with UVB) 9
Comparison of the highest average height recorded between the set-ups with (2A, 2B, 2C) and without UVB (1A, 1B, 1C)
Plant used Set-ups Highest average height
recorded
Difference between the two set-ups
Vigna radiata 1A (without UVB)
21 10.32A (with UVB) 9.7
Vigna angularis
1B (without UVB)
29 13
2B (with UVB) 16
Glycine max 1C (without UVB)
25 11.6
2C (with UVB) 13.4
The day of in which the plants grown under UVB died
Plant used Set-ups Day of death
Vigna radiata 2A (with UVB) 23
Vigna angularis 2B (with UVB) 22
Glycine max 2C (with UVB) 19
“Heating effect” of UVB is negligible……..
Initial temperature inside the acrylic box with switched-on UV lamp ( )℃
Temperature inside the acrylic box with switched on UV lamp after 3 hours ( )℃
26 26.5
The acrylic box
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