CryptomonadalesCryptomonadales®®: a unique species of Chlorella s: a unique species of Chlorella sorokiniana developed by MR. Shun-Te Wangorokiniana developed by MR. Shun-Te Wang
Nutritional Value of the Chlorella-Nutritional Value of the Chlorella-CryptomonadCryptomonad
ale ale ®® Product Product::Perfect health food for 21Perfect health food for 21stst century century
The first certificate of algae health food in Taiwan The first certificate of algae health food in Taiwan
Lack cell wall, easily and almost fully ( 90% ) absorbed
Perfect nutritional composition:Protein : CHO : lipid =60:14:8
Optimal combination of fatty acid: saturated FA: mono-unsaturated: polyunsaturated =
0.8:1.5:1.0
Chlorophyll: 4%Vitamin A, B, C, E
MineralsTrace elements: Zinc ( 70 mg% ), Selenium: 80mg%
Reports on the effects of CryptomonadalesReports on the effects of Cryptomonadales on medicines: on medicines: 引藻的醫療研究報告引藻的醫療研究報告
Crypto- can reduce the levels of cholesterol and low-densit
y lipoprotein ( LDL ) for up to 15-20% in one month
Regulation of blood glucose and glycosylated Hgb to nearl
y normal in 3-6 months
Crypto- can modulate immune function ( contact dermatiti
s, psoriasis, rheumatoid arthritis, and wound healing ) 。
Crypto- contains phycocyanin and has anti-viral effects
Potential prophylactic effects for cancers 。
引藻可降低自願受測者血中膽固醇引藻可降低自願受測者血中膽固醇A. Cholesterol
165
170
175
180
185
190
195
200
205
210
215
before 2 weeks 4 weeks after
chol
este
rol (
mg/
ml)
** 資料來自國衛院 蘇益仁主任
PPARs ( peroxisome Proliferator Activated Receptor ): the most promising molecules for drug development in 21th century
Peroxisome proliferator-activated rPeroxisome proliferator-activated receptor pathway diagrameceptor pathway diagram
From Wikipedia, the free encyclopedia
Biomedical effects of PPAR agonists: FattBiomedical effects of PPAR agonists: Fatty acid is the natural ligand for PPARsy acid is the natural ligand for PPARs
Effects of PPAR agonist on metabolic syndrome ( Effects of PPAR agonist on metabolic syndrome ( diabetes, obesity, hyperlipidemia, and hypertensiondiabetes, obesity, hyperlipidemia, and hypertension ) )
** 資料改編自 Science 2003;302:406
PPAR Agonist Activities in ChlorellPPAR Agonist Activities in Chlorella-a-CryptomonadalesCryptomonadales®® and Its Biomedi and Its Biomedi
cal Applicationcal Application
Ih-Jen Su, MD, PhDIh-Jen Su, MD, PhDDivision of Clinical Research, Division of Clinical Research,
National Health Research Institutes, National Health Research Institutes, TaiwanTaiwan
Extraction of PPARs-containing Fraction from Cryptomonades
** 資料來自國衛院 生技製藥組
Effects of Crypto- onPPARs expression
Tubulin
PPARα
Control Feno- GLA EPA cryp. fibrate tablet
* Fenofibrate : 50 μg/ml
(as PPARα control)
* GLA : γ-Linolenic Acid
* EPA : Icosapentaenoic acid
* cryp. tablet :引藻片*引藻處理濃度: 2 mg/ml
0
0.5
1
1.5
2
2.5
3
3.5
1數列 1 1.25 1.21 1.19 2.87
Control Fenofibrate GLA EPA cryp. tablet
Pilot trial on psoriatic arthritis
Improvement of arthritis and skin lesions
** 資料來自成大醫學院皮膚科 李玉雲醫師 ,林旻憲醫師
曾接受數種治療方式但未獲改善之患者 , 服用引藻 10 周以上評估其關節炎狀況
0 2 4 6 8 10 12 14 16 18 20 22 240
10
20
30
40
Time (week)
PA
SI s
core
°®Å~©ÊÃö¸`ª¢¯kµh«ü¼Æ
Before 1 month 2 month 3 month0.0
2.5
5.0
7.5
10.0Before1 month2 month3 month
Time after treatment
Pai
n s
core
Crypto-PPAR induce suppression of cytCrypto-PPAR induce suppression of cytokine tumor necrosis factor-alphaokine tumor necrosis factor-alpha
Immune mdulation and anti-inflammatory effectsImmune mdulation and anti-inflammatory effects
0 10 20 30 40 50 600
100
200
300 Control
TPA 2.5ng/ml
TPA+Troglitazone
TPA+Cryp 0.25
TPA+Cryp 1
TPA ³B²z24¤p®É«á¦A¥[¤JTroglitazone,Cryp.
Time (hour)
TN
F (
pg
/ml)
Crypto-PPARs can effectively reduce viral loCrypto-PPARs can effectively reduce viral load in animal modelad in animal model
0
10000
20000
30000
40000
50000
60000
d0 d6 d12 d17 d20 d24 d27 d31 d34 d38
Time (day)
Vira
l loa
d (c
opie
s/m
l)
PBS 64403
PBS 54409
HVP 59603
HVP 66007
Cryp 8d + HVP 59901
Cryp 8d + HVP 60503
Cryp 8d + HVP 55301
HVP + Cryp 7d 59501
HVP + Cryp 7d 66503
HVP + Cryp 7d 60501
Crypto-PPAR can reduce the Crypto-PPAR can reduce the oxidative stress and genomic oxidative stress and genomic
instability: instability: anti-aging and cosmeticsanti-aging and cosmetics
Contro
lBFA
Trog
litaz
one
Feno
fibra
te
BFA +
Cryp
Cryp
BFA +
Trog
litaz
one
BFA +
Feno
fibra
te
PPAR α53KD
GRP9494KD
PPAR γ54.5KD57.6KD
eIF240KD
IRE1110KD
GADD15330KD
PERK125KD
P-JNK54KD46KD
JNK
PPARs agonists: the most promising drugs; PPARs agonists: the most promising drugs; Chlorella-Cryptomonadales the best natural Chlorella-Cryptomonadales the best natural
product in the 21product in the 21stst century century
Metabolic syndrome:Metabolic syndrome: hyperlipidemia hyperlipidemia 、 、 diabetesdiabetes 、、 hypertensionhypertension 、、 obesityobesity
Immune diseases: arhtritis, eczema, allergy Immune diseases: arhtritis, eczema, allergy viral infection: influenza, enterovirus, viral infection: influenza, enterovirus,
? HIV/AIDS , ? hepatitis? HIV/AIDS , ? hepatitis
Aging: oxidative stress, cosmeticsAging: oxidative stress, cosmetics
Cancer prevention and therapyCancer prevention and therapy
Adipocyte Differentiation AssayFor PPAR-gamma ligand
** 資料來自國衛院生技製藥組
CE 1 CE 2 CE 3 CE 4 CE 5 CE 6 CE 7 CE 8
%
0
50
100
150
200
CE 3-1 CE 3-2 CE 3-3 CE 3-4 CE 3-5 CE 3-6
%
0
20
40
60
80
100
g/ml
100 101 102
% o
f m
axim
um
0
20
40
60
80CE 1CE 2CE 3CE 4CE 5CE 6CE 7CE 8
g/ml
100 101 102
% o
f m
ax
imu
m
0
20
40
60
80CE 3-1CE 3-2CE 3-3CE 3-4CE 3-5CE 3-6
** 資料來自國衛院生技製藥組
Extracts of Cryptomonadales contain high concentration of allophycocyanin: effective fo
r anti-viral
Nature Rev Immunol. 2002; 2:748-759
Molecular mechanism of immune modulation Molecular mechanism of immune modulation and anti-inflammation by PPARsand anti-inflammation by PPARs
Index molecule in Crypto-and quality assurance
引藻片的品管檢驗 次亞麻油酸 (Linolenic Acid): 18mg/gm Free of bacteria and safety levels of heavy metals Stability in 3 months at room temperature
** 資料來自工研院
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