For pathway key and background please visit:
cellsignal.co/EMT2
Contribution of Soluble Factors to EMT
UbUb
GS
K-3
β
Smad2
Smad3SARA
Fyn
PKC
Cri
pto
GRP78BP
Smad6/7
TGF-
βR1
TGF-
βR2
Smad1
Smad5
Smad8/9
Smad1
Smad5
Smad8/9
Sm
ad4
Sm
ad4
Wnt
Wnt
Wnt5B Wnt5A
CaMKI
CaMKII
MMP7
MMP3
AP
C
CK
1
Axin
β-C
aten
in
WTXPP2A
NIC
DT
M
NIC
DT
M
NIC
D
TMγ-Secretase
Smurf2
Smurf1
β-Catenin
β-Catenin
SlugSnail ZEB1/2Twist1
GSK-3FoxC2
Stat3
Ras Rac1
CDC42
p38
Raf JNK
MEK1/2
Erk1/2
β-Catenin
Transcriptional Induction of EMT Transcriptional Induction of EMT
NoncanonicalWnt Signaling
Canonical TGF-βSignaling
CanonicalWnt
Signaling
Proteolytic Cleavage
Jagged 1/2Delta 1/4
Proteasomal Degradation
TGF-β RTK Wnt BMP Notch
ClaudinsOcculidinsE-Cadherin
FibronectinVitronectinN-Cadherin
SPARCIntegrin αV
ClaudinsOccludinsE-CadherinCytokeratins
FibronectinVitronectinN-CadherinCollagen
MMPsTwistZEB1ZEB2
Cri
pto
TGF-
βR1
TGF-
βR2
Smad2
Smad3
ExpressionRepressionZO-1Cadherin
VitronectinN-CadherinsMMPs
ExpressionRepressionExpressionRepression
TGF-β RTK Wnt BMP
Cytoplasm
Nucleus
Frizzled 2 Frizzled 2 Frizzled Frizzled
β-Catenin
PI3K PLC
GSK-3β
Akt
BMP
RTK
RTK
β-Catenin
Sox10NICD N
ICD
KDM5A
CSL
KDM5A
CSL SKIP
MAML
HAT
Sox9NF-κB
NF-κB
TGF-β1,2,3 ADAM/TACE
β-TrCP
Lig
and
E-Cadherin
Ca2+
Actin RemodelingStress Fiber Formation
Increased Motility
β-Catenin Destruction
Complex
TCF
β-Catenin
Groucho
Sox9Lef1/Tcf1
Bcl9
CBP
Lef1/Tcf1
Groucho
HDAC
β-Catenin
No
tch
TGF-β RTK Wnt ECM/Itg
Contribution of Soluble Factors to EMT
rev. 08/16/18
For pathway key and background please visit:
APPLICATIONS & REACTIVITY KEY: WB Western Blotting / IP Immunoprecipitation / IHC Immunohistochemistry / IF Immunofluorescence / F Flow Cytometry / ChIP Chromatin Immunoprecipitation / -IC Immunocytochemistry / -P Paraffin / -F Frozen / E-P Peptide ELISA / H human / M mouse / R rat / Hm hamster / Mk monkey / C chicken / Mi mink / Dm D. melanogaster / X Xenopus / Z zebrafish / B bovine / Dg dog / Pg pig / Sc S. cerevisiae / All all species expected / ( ) 100% sequence homology
18-C
EL-1
2815
-FLY
2-E1
For R
esea
rch
Use
Only
. Not
For
Use
In D
iagn
ostic
Pro
cedu
res.
cellsignal.co/EMT2
© 2018 Cell Signaling Technology, Inc. Cell Signaling Technology, CST, and XP are trademarks of Cell Signaling Technology, Inc. U.S. Patent No. 5,675,063. All other trademarks are the property of their respective owners.
Key Products for the Contribution of Soluble Factors to EMTVisit www.cellsignal.com for a full listing of antibodies, related kits, and reagents.
ANTIBODIES & KITS APPLICATIONS REACTIVITY
#4147 Cleaved Notch1 (Val1744) (D3B8) Rabbit mAb WB, IP, ChIP H, M, R
#8658 Notch Receptor Interaction Antibody Sampler Kit
#2041 PP2A A Subunit (81G5) Rabbit mAb WB, IHC-P, IF-IC H, M, R, Mk
#4953 PP2A B Subunit Antibody WB, IP, IHC-P, IF-IC, F H,M, R, Mk, (C)
#2038 PP2A C Subunit Antibody WB, IP, IHC-P, IF-IC, F H, M, R, Mk, Dm, (C, Pg)
#5313 RBPSUH (D10A4) XP® Rabbit mAb WB, IHC-P, ChIP H, M, R, Mk
#9585 Slug (C19G7) Rabbit mAb WB, IP, IF-IC, F H, M
#6944 Smad1 (D59D7) XP® Rabbit mAb WB, IP, ChIP H, M, Mk, (X, B)
#13820 Phospho-Smad1 (Ser463/465)/Smad5 (Ser463/465)/Smad9 (Ser465/467) (D5B10) Rabbit mAb
WB, IP, IF-IC, F H, M, R, (Mk)
#12656 Smad 1/5/9 Antibody Sampler Kit
#5339 Smad2 (D43B4) XP® Rabbit mAb WB, IP, IF-IC, F, ChIP H, M, R, Mk
#18338 Phospho-SMAD2 (Ser465/Ser467) (E8F3R) Rabbit mAb
WB, IP, IF-IC, F, ChIP H, M, R
#8685 Smad2/3 (D7G7) XP® Rabbit mAb WB, IP, IF-IC, F, ChIP, ChIP-seq
H, M, R, Mk
#12747 Smad2/3 Antibody Sampler Kit
#9523 Smad3 (C67H9) Rabbit mAb WB, IP, IF-IC, F, ChIP,ChIP-seq
H, M, R, Mk, (X, Z, B)
#46535 SMAD4 (D3R4N) XP® Rabbit mAb WB, IP, IHC-P, IF-IC, F, ChIP, ChIP-seq
H, M, R, Mk
#12534 Smad5 (D4G2) Rabbit mAb WB, IP, ChIP H, M, R, Mk
#3879 Snail (C15D3) Rabbit mAb WB, IP H, M, R, Mk
#82630 Sox9 (D8G8H) Rabbit mAb WB, IHC-P, IF-IC, F H, (M, R)
#89356 Sox10 (D5V9L) Rabbit mAb WB, IP, IF-IC H
#3976 TACE Antibody WB, IP H, Mk
#3396 TCF8/ZEB1 (D80D3) Rabbit mAb WB, IP H, (M, R)
#3711 TGF-ß Antibody WB H, M, R
#79424 TGF-ß Receptor II Antibody WB H, M, R
#46702 TWIST1 Antibody WB, IP H
#2721 Wnt3a (C64F2) Rabbit mAb WB, IP M, (H, R)
#2530 Wnt5a/b (C27E8) Rabbit mAb WB H
Jagged1 (D4Y1R) XP® Rabbit mAb #70109 Flow cytometric analysis of Jurkat cells (blue) and MKN45 cells (green) using #70109. Anti-rabbit IgG (H+L), F(ab’)2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Jagged1
Even
ts
SMAD4 (D3R4N) XP® Rabbit mAb #46535 IHC analysis of paraffin-embedded human prostate carcinoma using #46535.
ANTIBODIES & KITS APPLICATIONS REACTIVITY
#4151 ADAM9 (D64B5) Rabbit mAb WB H, M, R, Mk
#2504 APC Antibody WB, IP H, (M, R,Mk)
#2087 Axin1 (C76H11) Rabbit mAb WB, IP, IHC-P H, M
#2151 Axin2 (76G6) Rabbit mAb WB, IP H
#13116 N-Cadherin (D4R1H) XP® Rabbit mAb WB, IP, IHC-P, IF-IC H, M
#4436 CaMKII (pan) (D11A10) Rabbit mAb WB H, M, R
#9561 Phospho-ß-Catenin (Ser33/37/Thr41) Antibody
WB H, M, R, Mk, (C, X, Z, B, Dg, Pg)
#4176 Phospho-ß-Catenin (Ser675) (D2F1) XP® Rabbit mAb
WB, IP, IF-F, IF-IC H, M, R, (M, R, C X, Z)
#7389 CBP (D6C5) Rabbit mAb WB, IP, IF-IC, ChIP, ChIP-seq
H, M, R, Mk
#2466 Cdc42 (11A11) Rabbit mAb WB H, M, R, B, (X)
#2655 CK1 Antibody WB H, M, R, (C, X, Z, B)
#96406 DLL4 (D7N3H) Rabbit mAb WB, IP, IF-IC H
#4023 Fyn Antibody WB H, M
#70109 Jagged1 (D4Y1R) XP® Rabbit mAb WB, IP, IHC-P, F H, M, Mk, (R, Hm)
#2210 Jagged2 (C23D2) Rabbit mAb WB, IP H, R
#3876 JARID1A (D28B10) Rabbit mAb WB, IP H, M, (R, B)
#25807 Keratin 5 (D4U8Q) Rabbit mAb WB, IF-IC, F H
#4465 Keratin 7 (D1E4) XP® Rabbit mAb WB, IF-IC, F H
#4546 Keratin 8/18 (C51) Mouse mAb WB, IHC-P, IF-IC F H, Mk
#12434 Keratin 17/19 (D4G2) XP® Rabbit mAb WB, IHC-P, IF-IC H, M, R
#4548 Keratin 18 (DC10) Mouse mAb WB, IHC-P, IF-IC,F H
#4558 Keratin 19 (BA17) Mouse mAb WB, IHC-P H
#13063 Keratin 20 (D9Z1Z) XP® Rabbit mAb WB, IHC-P, IF-IC, F H
#4545 Pan-Keratin (C11) Mouse mAb WB, IHC-P, IF-P, IF-IC, F H, M, R, Mk
#12166 MAML1 (D3K7B) Rabbit mAb WB IP, ChIP, ChIP-seq H, M, R
#6988 MAML2 (D41E6) Rabbit mAb WB, IP H, M, R
#8242 NF-κB p65 (D14E12) XP® Rabbit mAb WB, IP, IHC-P, IF-IC, F, ChIP, ChIP-seq
H, M, R, Hm, Mk, Dg
#3608 Notch1 (D1E11) XP® Rabbit mAb WB, IP, IHC-P, ChIP H, M, R
#5732 Notch2 (D76A6) XP® Rabbit mAb WB, IP, IF-IC, F H, M, R
Slug (C19G7) Rabbit mAb #9585 Confocal IF analysis of A204 cells (left) and PANC-1 cells (right) using #9585. Actin filaments have been labeled with DY554 phalloidin (red).
Snail (C15D3) Rabbit mAb #3879 WB analysis of extracts from various cell lines using #3879.
kDa
Snail
200
140
100
80
6050
40
30
20
HC T116
HeLa
NIH/3T
3
Rat2 COS-7
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