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MIKROBIOLOGI
Mekanisme Dasar
Penyakit
Bagian Mikrobiologi
Fakultas Kedokteran Universitas Hasanuddin
2014
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KEL ASISTEN NIM ( C 111 ....)
1 MUHAMMAD FUDAIL 09 136, 10 110, 13 00113 0!6
! "RI #AH$U%I R& 10 13', 10 !63, 13 0!(13 0)3
3 *OR+I% KUR%IA#A% & 10 !!(, 10 !'0, 13 0)-13 0((
- A& MUH& FADLILLAH F& 10 !99, 10 30', 13 0('
13 10!) MAR$AM BURHA%UDDI% 10 31-, 10 33!, 13 103
13 30)
6 MUIAH MUFIHAURRAHMA% 10 3!-, 10 3!6, 13 30(13 33-
( MUH& RAHMA RIDHA 10 3!', 10 33!, 13 33)13 36(
' #A% A"H.KAL %A/UA% BI% #A%A/MI
10 33-, 13 36'13 )!(
9 HAFI/ HA/#A% MOHAMMAD %ORI/A% 10 339, 13 )!'13 ))-
10 "II AI"HAH KHAIRUDI% 10 3-6, 13 ))(13 (0-
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TATA-TERTIB LABORATORIUMMIKROBIOLOGI
Laki laki : Memakai eana 2anan4 kain, n5 255 sirt,ram78t tiak 75e kena kera 7a8
Perem!a" :Memakai r5k 2anan4 kain, n5 255 sirt,
ram78t iikat ke atas& Ba4i y4 7eri7a7, i7a7 imas8kkani aam as a7 Ala# $a"% &ar!' ia*a 'aa# rak#ik!m :
*as a7
ID :ar
Pen8nt8n 2raktik8m ; F5t5 -
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BAK.RI
Bakteri aaa ma8k i82,7erse t8n44a kei en4an
imeter kirakira 1 m, yan4mas8k aam 455n4an 2r5tistarena, Pr5kari5tik, Inti anya
7r2 m7aran kr5m5s5m tan2amem7ran inti
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"e Bakteri
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"RUKUR BAK.RI
Dinin4 "e Mem7ran "e "it52asma %8e5si Ka2s8
Fa4e Pii>Fim7riae "25ra
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Fa4e
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M5r?554i Bakteri
58s
7aii
i255i
stre2t55i
.
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sarina
Lan8tan
sta2y55i tettraen
557aii
.
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Lan8tan
Fiament58s?5rm
i7ri5
F8si?5rm 7aii
s2ira
.
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+ia%",'i"% i" Mir,i,l,%$
"2eimen @ De2en 5n sym2t5ms an timin4 Urine, 755, 28s, s2ina C8i, s28t8m, 5r st55
P.#AR%AA% :8t8res EB55 A4ar,%8trient a4ar, BHIA,
Ma:5nkey, "" A4ar, :B" Bi5emia ests E"I, "IM, +P, Mety Re,
:itrat, Urea, KH test %8ei Ai Met5s EP:R, y7riiati5n,
75ts Anti75y Meiate E.LI"A, sie>ate+OG." PRO"KAU.R EMR>+P
Mety Re
E; @Mera E @
+54es Pr5ska8er
E; @ Mera E @
Tujuan : Untuk mengetahui apakah bakteri memfermentasikanglukosa atau tidak.
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:IRA. UR.A H$DROL$"I"
:itrate
E; @ Bir8 E @ Hia8
Urea Hyr5ysis
E; @Pink E @ K8nin4
"UGAR F.RM.%AIO%
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"UGAR F.RM.%AIO%."
Lat5seFermentati5n
"8r5se
Fermentati5n G85se
Fermentati5n Mannit5
E; @ K8nin4 E @ Mera
Tube 1: Negative acid /Negative gas
Tube 2A: Must incubate longer (ambiguous result)
Tube 2B: Positive acid /Negative gas
Tube 3A: Positive acid/ Positive gas
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Special selective and diferential agar medium:
1.
M C,"ke$ a%arla#e0. SS a%ar la#e
5. TCBS
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Mc Conkey Agar
MaC,"ke$ a%ar is a 8t8re mei8mesi4ne t5 4r5= Gramne4atie7ateria an stain tem ?5r at5se?ermentati5n&
In4reients@ Bile Sal# Et5 ini7it m5st Gram25sitie
7ateria, e
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Ma5nkey a4ar =itat5seEe?t an n5n
at5seEri4t ?ermenters
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PRI%:IPL.
La#,'e By 8tiiin4 te at5se aaia7e in te
mei8m, La; 7ateria s8 asEscherichia coli, Enterobacter anKlebsiella =i 2r58e ai, =i5=ers te 2H 5? te a4ar 7e5= 6&' anres8ts in te a22earane 5? re>2ink
55nies& e 7ie sats 2rei2itate in teimmeiate nei475r55 5? te 55ny,a8sin4 te mei8m s8rr58nin4 te55ny t5 7e5me ay&
http://en.wikipedia.org/wiki/Escherichia_colihttp://en.wikipedia.org/wiki/Enterobacterhttp://en.wikipedia.org/wiki/Klebsiellahttp://en.wikipedia.org/wiki/Acidhttp://en.wikipedia.org/wiki/Bacterial_colonyhttp://en.wikipedia.org/wiki/Bacterial_colonyhttp://en.wikipedia.org/wiki/Acidhttp://en.wikipedia.org/wiki/Klebsiellahttp://en.wikipedia.org/wiki/Enterobacterhttp://en.wikipedia.org/wiki/Escherichia_coli7/25/2019 BMD Mikro.pptx
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La#,'e- %5nLat5se ?ermentin4 7ateria s8 as
Salmonella, Proteus species,
Pseudomonas aeruginosa an Shigella ann5t8tiie at5se, an =i 8se 2e2t5ne instea&
is ?5rms amm5nia, =i raises te 2H 5?te a4ar, an eas t5 te ?5rmati5n 5?
=ite>55ress 55nies ?5rme in te 2ate&B8t tey an as5 55k 45en t5 7r5=n =itark enters& ey are ir8ar 55nies anarran4e ran5my&
http://en.wikipedia.org/wiki/Salmonellahttp://en.wikipedia.org/wiki/Proteus_(bacterium)http://en.wikipedia.org/wiki/Pseudomonas_aeruginosahttp://en.wikipedia.org/wiki/Shigellahttp://en.wikipedia.org/wiki/Peptonehttp://en.wikipedia.org/wiki/Ammoniahttp://en.wikipedia.org/wiki/Agarhttp://en.wikipedia.org/wiki/Agarhttp://en.wikipedia.org/wiki/Ammoniahttp://en.wikipedia.org/wiki/Peptonehttp://en.wikipedia.org/wiki/Shigellahttp://en.wikipedia.org/wiki/Pseudomonas_aeruginosahttp://en.wikipedia.org/wiki/Proteus_(bacterium)http://en.wikipedia.org/wiki/Salmonella7/25/2019 BMD Mikro.pptx
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SS agar plate:
In4reients@ %8trient a4ar 100m Lat5se 1&004 Bie sats 0&')4
ini7it5r
"5i8m itrate 0&')4ini7it5r
"5i8m ti5s8?ate 0&')4
10 ?erri itrate 1m 1 %e8tra re 0&!)m
iniat5r
1 7riiant 4reen 0&033mini7it5r
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(
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(T&i,'!l/a#e-Ci#ra#e-Bile-S!r,'e)
Use ?5r8t8re Vibriocholerae
$e5= 55nies?5r i7ri5s 8et5 5
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TCBS A%ar: $east .
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e 7ie sats in te meia ini7it te4r5=t 5? 4ram25sitie mir57este 2resene 5? s8r5se a5=s ?5r te
ierentiati5n 5? t5se i7ri5s, =ian 8tiie s8r5se =it te ai 5?7r5mtym5 78e, an tym5 78e
iniat5rs& e i4 '&6 2H 5? :B"A4ar s822resses 5ter intestina C5ra=ie a5=in4 8nini7ite 4r5=t 5?
i7ri5s&
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M.DIUM LO#.%".I% *.%".%
K55ni 2aa Meia LQ=enstein*ensen akan tam2ak2ert8m78an aam =akt83- min448 7er=arna 28tikek8nin4k8nin4an, 2erm8kaankerin4, kasar Etiak iin an
ra28 en4an te2i yan4 tiak7erat8ran Ese2erti 78n4a k5&e2i @ 7er4eri4i
60
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K5m25sisi Meia LQ=enstein*ensen as2ara4in, KH!PO-,M4
sitrat, M4 s8?at, 4iserin 28ti te8r ayam>7e7ek
maakit ia8 steri !
61
M.DIUM LO#.%".I% *.%".%
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M.DIUM RA%"POR
S#!ar# mei!m@ i2akai 8nt8k trans25rtasis2eimen yan4 men4an8n4 7akteri 25siti?
Gram an ne4ati? Gram& Carr$ a" Blair mei!m@ i2akai 8nt8k
trans25rtasi tina ata8 a28san ret8m yan4i8ri4ai men4an8n4 Vibrioata8
Enterobacteriaceaeyan4 2at54en&
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Mei8m enriment 8nt8k 7akterienterik@ Air e"#," alkali': 8nt8k Vibrio s2eies
aam tina Sele"i#e: Salmonella anShigella s2eies
aam tina&
Mei!m ile e#,": 8nt8k Salmonella
typhiaam ara
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Air 2ent5n akais
:arry an Bair Mei8m
"t8art Mei8m O< Bie"eenite
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KEPEKAAN ANTIBIOTIK
Lak8kan2en48k8ran
iameter 5naerni i sekitaris anti7i5tikE5na ini7isi an
7anin4kan ta7er88kan 8nt8kmenent8kaninter2retasinya&
80
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88
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.RIMA KA"IH
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"IAPR."PO% SSSSSSS
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R."PO% A
ULI" %AMA, %IM ,
K.LOMPOK DA%A"I".%
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1
A& A2a it8 7akteriTB& "e78tkan str8kt8r ari 7akteri T
Emin )
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!
A
B
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3
A& "e78tkan rea4enrea4en yan4i48nakan 2aa 2e=arnaan taanasamT
B& Inter2retasi ariBA ; @BA @
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-
A& A2a ?8n4si ari M5rantTB& A2a 2er7eaan met5e /ie J
%eesen an ani 2aa 2e=arnaan
taan asamTT
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)
A& A2a nama tes7i5kimia
isam2in4TB& A2a J a2a saa
yan4 a2at iiat
2aa tes 7i5kimiaterse78tT
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KUMPUL SSSSSSS
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R."PO% B
ULI" %AMA, %IM ,
K.LOMPOK DA%A"I".%
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1
".BUKA% 3 P.RB.DAA% BAK.RIDA% *AMUR T
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!
A& Pe=arnaan
a2a yan4isam2in4T
B& A2a J a2a at
=arna yan4i48nakan T
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3
A& "e78tkan rea4enrea4en yan4i48nakan 2aa 2e=arnaan 4ram T
B& Inter2retasi ari
Gram ; @Gram @
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-
"e78tkan ! minima 7akteri yan4taan
asamT
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)
a. Apa nama tes
biokimia
disamping ?
b. Apa apa saja
yang dapat dilihat
pada tes biokimiatersebut?
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KUMPUL SSSSSSS